Yıl: 2021 Cilt: 37 Sayı: 2 Sayfa Aralığı: 348 - 355 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Effects of Bendiocarb Insecticide on Lipid Peroxidation, Antioxidant Enzymes and DNA Damage in Human Leucocytes

Öz:
In this study, we aimed to determine the toxic effect of increasing doses ofBendiocarb, an insecticide from the carbamate group, on human blood cells in vitro viaby Comet test, determination of total antioxidant capacity, antioxidant enzyme activityand malondialdehyde (MDA) level. Leukocyte isolation was performed from bloodsamples taken from six healthy male individuals who did not smoke and use alcohol andwere not exposed to any chemicals in the working environment, and then theirleukocytes exposed to increasing doses of Bendiocarb (20, 40, 80, 160 µg / mL). Comettest, iron reduction antioxidant capacity (FRAP), 2-2'azinobis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS/TEAC) analysis with superoxide dismutase (SOD), catalase (CAT),glutathione peroxidase (GPx) and MDA were evaluated. At the end of the study, it wasdetermined that antioxidant enzyme levels decreased significantly due to dose increase,whereas MDA levels increased significantly in all doses (p<0.05). According to FRAP andTEAC methods, the antioxidant capacity of blood cells decreased significantly inincreasing doses of Bendiocarb compared to the control group (p<0.05). In the Comettest, DNA tail percentage, length and moment were increased significantly in increasingdoses of Bendiocarb and DNA damage was detected (p<0.05). This study is a preliminarystudy on this subject, and it has been shown in our study that the uncontrolled use ofBendiocarb pesticide in nature will have harmful effects on the environment and livingthings. We believe that the research will contribute to the literature and shed light onfuture comprehensive studies.
Anahtar Kelime: Bendiocarb Genotoxicity Comet Assay Antioxidant Enzymes Pesticides

Bendiokarb İnsektisitinin İnsan Lökositlerinde Lipid Peroksidasyonu, Antioksidan Enzimler ve DNA Hasarı Üzerindeki Etkileri

Öz:
Bu çalışma da karbamat grubundan bir insektisit olan Bendiokarb’ın artan dozlarının insan kan hücreleri üzerine in vitro koşullarda toksik etkisini komet testi, total antioksidan kapasite tayini, antioksidan enzim aktivitesi ve malondialdehit (MDA) seviyesini değerlendirmek suretiyle belirlemeyi amaçladık. Sigara ve alkol kullanmayan, çalıştığı ortamda herhangi bir kimyasal maddeye maruz kalmayan sağlıklı altı erkek bireyden heparinli tüplere alınan kan örneklerinden lökosit izolasyonu yapılmış ve lökositleri artan dozlarda Bendiokarb’a (20, 40, 80, 160 µg/mL) maruz bırakılmıştır. Komet testi, demir indirgeme antioksidan kapasitesi (FRAP), 2-2'azinobis 3- ethilbenzotiazolin-6-sülfonik asit (ABTS/TEAC) analizleri ile antioksidan enzimler olan süperoksit dismutaz (SOD), katalaz (CAT), glutatyon peroksidaz (GPx) ve lipid peroksidasyon göstergesi olan MDA düzeyleri üzerine etkileri değerlendirilmiştir. Çalışma sonunda antioksidan enzim düzeylerinin doz artışına bağlı olarak anlamlı azaldığı, tüm dozlarda ise MDA düzeylerinin anlamlı olarak arttığı belirlenmiştir (p<0.05). FRAP ve TEAC yöntemlerine göre, kan hücrelerinin antioksidan kapasitesi, kontrol grubuna göre artan Bendiokarb dozlarında anlamlı olarak azalmıştır (p<0.05). Comet testinde artan Bendiokarb dozlarında DNA kuyruk yüzdesi, uzunluğu ve momenti anlamlı olarak artmış ve DNA hasarı tespit edilmiştir (p <0.05). Bu çalışma bu konuda yapılmış bir ön çalışma niteliği taşımakta olup, çalışmamızda Bendiokarb pestisitinin doğada kontrolsüz kullanımının çevreye ve canlılara zararlı etkileri olacağı gösterilmiştir. Araştırmanın literatüre katkı sağlayacağı gibi bundan sonraki kapsamlı çalışmalara da ışık tutacağı düşünülmektedir.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA Doğanyiğit İ, OZTÜRK KÜP F (2021). Effects of Bendiocarb Insecticide on Lipid Peroxidation, Antioxidant Enzymes and DNA Damage in Human Leucocytes. , 348 - 355.
Chicago Doğanyiğit İsrafil,OZTÜRK KÜP FATMA Effects of Bendiocarb Insecticide on Lipid Peroxidation, Antioxidant Enzymes and DNA Damage in Human Leucocytes. (2021): 348 - 355.
MLA Doğanyiğit İsrafil,OZTÜRK KÜP FATMA Effects of Bendiocarb Insecticide on Lipid Peroxidation, Antioxidant Enzymes and DNA Damage in Human Leucocytes. , 2021, ss.348 - 355.
AMA Doğanyiğit İ,OZTÜRK KÜP F Effects of Bendiocarb Insecticide on Lipid Peroxidation, Antioxidant Enzymes and DNA Damage in Human Leucocytes. . 2021; 348 - 355.
Vancouver Doğanyiğit İ,OZTÜRK KÜP F Effects of Bendiocarb Insecticide on Lipid Peroxidation, Antioxidant Enzymes and DNA Damage in Human Leucocytes. . 2021; 348 - 355.
IEEE Doğanyiğit İ,OZTÜRK KÜP F "Effects of Bendiocarb Insecticide on Lipid Peroxidation, Antioxidant Enzymes and DNA Damage in Human Leucocytes." , ss.348 - 355, 2021.
ISNAD Doğanyiğit, İsrafil - OZTÜRK KÜP, FATMA. "Effects of Bendiocarb Insecticide on Lipid Peroxidation, Antioxidant Enzymes and DNA Damage in Human Leucocytes". (2021), 348-355.
APA Doğanyiğit İ, OZTÜRK KÜP F (2021). Effects of Bendiocarb Insecticide on Lipid Peroxidation, Antioxidant Enzymes and DNA Damage in Human Leucocytes. Erciyes Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 37(2), 348 - 355.
Chicago Doğanyiğit İsrafil,OZTÜRK KÜP FATMA Effects of Bendiocarb Insecticide on Lipid Peroxidation, Antioxidant Enzymes and DNA Damage in Human Leucocytes. Erciyes Üniversitesi Fen Bilimleri Enstitüsü Dergisi 37, no.2 (2021): 348 - 355.
MLA Doğanyiğit İsrafil,OZTÜRK KÜP FATMA Effects of Bendiocarb Insecticide on Lipid Peroxidation, Antioxidant Enzymes and DNA Damage in Human Leucocytes. Erciyes Üniversitesi Fen Bilimleri Enstitüsü Dergisi, vol.37, no.2, 2021, ss.348 - 355.
AMA Doğanyiğit İ,OZTÜRK KÜP F Effects of Bendiocarb Insecticide on Lipid Peroxidation, Antioxidant Enzymes and DNA Damage in Human Leucocytes. Erciyes Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2021; 37(2): 348 - 355.
Vancouver Doğanyiğit İ,OZTÜRK KÜP F Effects of Bendiocarb Insecticide on Lipid Peroxidation, Antioxidant Enzymes and DNA Damage in Human Leucocytes. Erciyes Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2021; 37(2): 348 - 355.
IEEE Doğanyiğit İ,OZTÜRK KÜP F "Effects of Bendiocarb Insecticide on Lipid Peroxidation, Antioxidant Enzymes and DNA Damage in Human Leucocytes." Erciyes Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 37, ss.348 - 355, 2021.
ISNAD Doğanyiğit, İsrafil - OZTÜRK KÜP, FATMA. "Effects of Bendiocarb Insecticide on Lipid Peroxidation, Antioxidant Enzymes and DNA Damage in Human Leucocytes". Erciyes Üniversitesi Fen Bilimleri Enstitüsü Dergisi 37/2 (2021), 348-355.