LC–MS/MS and RP–HPLC–UV Analysis and Antioxidant Activities of Arum italicum Miller Edible and Nonedible Tuber Parts
Yıl: 2021 Cilt: 6 Sayı: 3 Sayfa Aralığı: 294 - 301 Metin Dili: İngilizce DOI: 10.35229/jaes.864484 İndeks Tarihi: 29-07-2022
LC–MS/MS and RP–HPLC–UV Analysis and Antioxidant Activities of Arum italicum Miller Edible and Nonedible Tuber Parts
Öz: In the study, the phenolic components and antioxidant activities of edible andnonedible parts of tuber of Arum italicum plant were carried out to determine in methanolic andwater extracts. In addition, the antioxidant potential of these parts of the plant was associated withtheir phenolic composition. Total phenolic content, FRAP, CUPRAC and DPPH tests as radicalscavenging activity were performed to determine the antioxidant activity of the plant. Thephenolic profiles in the two parts of the tuber were determined in the prepared methanolic extractsby RP–HPLC–UV and LC–MS/MS. The highest values of the total phenolic content, FRAP,CUPRAC and % DPPH were measured in methanolic extracts of nonedible parts of the tuber as164 µg GAE/mL, 878 TEAC (µM), 0.064 TEAC (µM) and 19.41, respectively. Ferulic acid wasdetermined as the main phenolic compound in methanolic extracts of both the tuber parts byLC/MS-MS. However, luteolin and rutin phenolics were measured as the major compounds inthe edible and nonedible parts of the plant tuber with analysis based on RP-HPLC-UV,respectively
Anahtar Kelime: Arum italicum Miller Bitkisinin Yenilebilir ve Yenmeyen Yumru Parçalarının LC–MS/MS ve RP–HPLC–UV Analizi ve Antioksidan Aktiviteleri
Öz: Bu çalışmada, Arum italicum bitkisine ait yumrunun yenilebilir ve yenmeyen kısımlarından elde edilen metanolik ve sulu ekstraktların, fenolik içerikleri ve antioksidan aktiviteleri belirlenmiştir. Ek olarak, bitkinin bu kısımlarının antioksidan potansiyeli, fenolik kompoziyonu ile ilişkilendirilmiştir. Bitkinin antioksidan aktivitesini belirlemek için Toplam Fenolik Madde Miktarı, FRAP, CUPRAC ve radikal süpürme aktiviteyi belirleyen DPPH antioksidan aktivite testleri yapılmıştır. Bitki yumrusunun her iki kısmındaki fenolik profiller hazırlanan metanolik ekstraktlarında RP– HPLC–UV ve LC–MS/ MS cihazları kullanılarak belirlenmiştir. En yüksek toplam fenolik içerik, FRAP, CUPRAC ve % DPPH değerleri, yumrunun yenmeyen kısmının metanolik ekstraktında sırasıyla 164 µg GAE/mL, 878 TEAC(µM), 0.064 TEAC(µM) ve 19.41 olarak ölçülmüştür LC/MS-MS ölçümlerinde her iki yumru kısmının metanolik ekstraktlarında ferulik asit, ana fenolik bileşik olarak belirlendi. Bununla birlikte RP–HPLC–UV ölçümlerinde ise, bitki yumrusunun yenilebilir ve yenilmeyen kısımlarında sırasıyla luteolin ve rutin fenolikleri ana bileşikler olarak ölçülmüştür.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | AKAR Z, DEMİR Ç, Alkan O, Can Z, AKAR B (2021). LC–MS/MS and RP–HPLC–UV Analysis and Antioxidant Activities of Arum italicum Miller Edible and Nonedible Tuber Parts. , 294 - 301. 10.35229/jaes.864484 |
| Chicago | AKAR ZEYNEP,DEMİR ÇAĞLAR,Alkan Ozan,Can Zehra,AKAR BÜLENT LC–MS/MS and RP–HPLC–UV Analysis and Antioxidant Activities of Arum italicum Miller Edible and Nonedible Tuber Parts. (2021): 294 - 301. 10.35229/jaes.864484 |
| MLA | AKAR ZEYNEP,DEMİR ÇAĞLAR,Alkan Ozan,Can Zehra,AKAR BÜLENT LC–MS/MS and RP–HPLC–UV Analysis and Antioxidant Activities of Arum italicum Miller Edible and Nonedible Tuber Parts. , 2021, ss.294 - 301. 10.35229/jaes.864484 |
| AMA | AKAR Z,DEMİR Ç,Alkan O,Can Z,AKAR B LC–MS/MS and RP–HPLC–UV Analysis and Antioxidant Activities of Arum italicum Miller Edible and Nonedible Tuber Parts. . 2021; 294 - 301. 10.35229/jaes.864484 |
| Vancouver | AKAR Z,DEMİR Ç,Alkan O,Can Z,AKAR B LC–MS/MS and RP–HPLC–UV Analysis and Antioxidant Activities of Arum italicum Miller Edible and Nonedible Tuber Parts. . 2021; 294 - 301. 10.35229/jaes.864484 |
| IEEE | AKAR Z,DEMİR Ç,Alkan O,Can Z,AKAR B "LC–MS/MS and RP–HPLC–UV Analysis and Antioxidant Activities of Arum italicum Miller Edible and Nonedible Tuber Parts." , ss.294 - 301, 2021. 10.35229/jaes.864484 |
| ISNAD | AKAR, ZEYNEP vd. "LC–MS/MS and RP–HPLC–UV Analysis and Antioxidant Activities of Arum italicum Miller Edible and Nonedible Tuber Parts". (2021), 294-301. https://doi.org/10.35229/jaes.864484 |
| APA | AKAR Z, DEMİR Ç, Alkan O, Can Z, AKAR B (2021). LC–MS/MS and RP–HPLC–UV Analysis and Antioxidant Activities of Arum italicum Miller Edible and Nonedible Tuber Parts. JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES, 6(3), 294 - 301. 10.35229/jaes.864484 |
| Chicago | AKAR ZEYNEP,DEMİR ÇAĞLAR,Alkan Ozan,Can Zehra,AKAR BÜLENT LC–MS/MS and RP–HPLC–UV Analysis and Antioxidant Activities of Arum italicum Miller Edible and Nonedible Tuber Parts. JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES 6, no.3 (2021): 294 - 301. 10.35229/jaes.864484 |
| MLA | AKAR ZEYNEP,DEMİR ÇAĞLAR,Alkan Ozan,Can Zehra,AKAR BÜLENT LC–MS/MS and RP–HPLC–UV Analysis and Antioxidant Activities of Arum italicum Miller Edible and Nonedible Tuber Parts. JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES, vol.6, no.3, 2021, ss.294 - 301. 10.35229/jaes.864484 |
| AMA | AKAR Z,DEMİR Ç,Alkan O,Can Z,AKAR B LC–MS/MS and RP–HPLC–UV Analysis and Antioxidant Activities of Arum italicum Miller Edible and Nonedible Tuber Parts. JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES. 2021; 6(3): 294 - 301. 10.35229/jaes.864484 |
| Vancouver | AKAR Z,DEMİR Ç,Alkan O,Can Z,AKAR B LC–MS/MS and RP–HPLC–UV Analysis and Antioxidant Activities of Arum italicum Miller Edible and Nonedible Tuber Parts. JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES. 2021; 6(3): 294 - 301. 10.35229/jaes.864484 |
| IEEE | AKAR Z,DEMİR Ç,Alkan O,Can Z,AKAR B "LC–MS/MS and RP–HPLC–UV Analysis and Antioxidant Activities of Arum italicum Miller Edible and Nonedible Tuber Parts." JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES, 6, ss.294 - 301, 2021. 10.35229/jaes.864484 |
| ISNAD | AKAR, ZEYNEP vd. "LC–MS/MS and RP–HPLC–UV Analysis and Antioxidant Activities of Arum italicum Miller Edible and Nonedible Tuber Parts". JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES 6/3 (2021), 294-301. https://doi.org/10.35229/jaes.864484 |
