Investigation of Aloe Vera Barbadensis Miller Leaf Extract Effects On Glutamate and Glyphosate Induced Toxicity: In Vitro Study
Yıl: 2021 Cilt: 6 Sayı: 3 Sayfa Aralığı: 376 - 381 Metin Dili: İngilizce DOI: 10.35229/jaes.953830 İndeks Tarihi: 29-07-2022
Investigation of Aloe Vera Barbadensis Miller Leaf Extract Effects On Glutamate and Glyphosate Induced Toxicity: In Vitro Study
Öz: Aloe vera is one of the medicinal plants whose importance has been better understoodrecently with its antibacterial, antimicrobial, antioxidant and anticancer properties. Although it isknown that the aloe vera family has protective effects on neurons, the neuroprotective effect ofthe aloe barbadensis miller plant has not yet been fully explained. Glufosinate is structurallysimilar to glutamate and is an herbicide that blocks glutamine synthesis. Glutamate has beenshown to induce cyclooxygenase-2 (COX-2), which causes toxicity. In the present study, neuronculture was exposed to Aloe vera barbadensis Miller plant extracts plant (25, 50, 100, 200, 400,800, and 1600 µgr/ml doses) for 24 hours to protect against glufosinate (200 mM) and glutamate(10-5 mM) toxicity. After 24 hours, MTT, TAC, and TOS analyzes were performed and the resultswere revealed. In our study, it was seen that the aqueous extract of aloe barbadensis miller plant,glufosinate, and glutamate, could preserve the vitality of neurons (89% protection in AVB 400µgr/ml group). At the same time, it was seen that while increasing the antioxidant level in neurons,it decreased the oxidant level. The group that increases the antioxidant value best is AVB 400µgr/ml (the group that increases it 1.4 times). When the findings were evaluated, it was concludedthat aloe vera and its components may have a neuroprotective effect.
Anahtar Kelime: Aloe vera barbadensis Miller Yaprağı Ekstraktının Glutamat ve Glifosat Kaynaklı Toksisite Üzerindeki Etkilerinin Araştırılması: İn Vitro Çalışması
Öz: Antibakteriyel, antimikrobiyal, antioksidan ve antikanser gibi özellikleriyle son zamanlarda önemi daha da iyi anlaşılan Aloe vera şifalı bitkilerden biridir. Aloe vera ailesinin nöronlar üzerinde koruyucu etkileri olduğu bilinmesine rağmen aloe barbadensis miller bitkisinin nöroprotektif etkisi henüz tam olarak açıklanamamıştır. Glufosinat, yapısal olarak glutamata benzer ve glutamin sentetazı bloke eden bir herbisittir. Glutamatın toksisiteye neden olan siklooksijenaz-2'yi (COX-2) indüklediği gösterilmiştir. Ayrıca nöronlarda oksidatif strese neden olduğu bilinmektedir. Mevcut çalışmada, nöron kültürü, glufosinat (200 mM) ve glutamat (10-5 mM) toksisitesine karşı korunmak amacıyla 24 saat süreyle Aloe vera barbadensis Miller bitkisi ekstraktlarına (25, 50, 100, 200, 400, 800 ve 1600 µgr/ml) maruz bırakılmış olup, 24 saat sonunda MTT, TAC ve TOS analizleri yapılmıştır. Çalışmamızda aloe barbadensis miller bitkisinin sulu ekstraktının, glufosinat ve glutamat toksisitesine karşı nöron canlılığını koruyabildiği görülmüştür (AVB 400 µgr/ml grubunda %89 koruma). Aynı zamanda nöronlarda antioksidan seviyesini arttırırken oksidan seviyesini azalttığı gözlemlenmiştir. Antioksidan değerini en iyi artıran grup AVB 400 µgr/ml (1,4 kat artıran grup) olarak tespit edilmiştir. Elde edilen bulgular değerlendirildiğinde, aloe vera ve bileşenlerinin nöroprotektif etkisi olabileceği sonucuna varılmıştır.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Yilmaz A, TAGHİZADEHGHALEHJOUGHİ A, Hacimuftuoglu A, Türkmen A (2021). Investigation of Aloe Vera Barbadensis Miller Leaf Extract Effects On Glutamate and Glyphosate Induced Toxicity: In Vitro Study. , 376 - 381. 10.35229/jaes.953830 |
| Chicago | Yilmaz Aysegul,TAGHİZADEHGHALEHJOUGHİ Ali,Hacimuftuoglu Ahmet,Türkmen Aybike Investigation of Aloe Vera Barbadensis Miller Leaf Extract Effects On Glutamate and Glyphosate Induced Toxicity: In Vitro Study. (2021): 376 - 381. 10.35229/jaes.953830 |
| MLA | Yilmaz Aysegul,TAGHİZADEHGHALEHJOUGHİ Ali,Hacimuftuoglu Ahmet,Türkmen Aybike Investigation of Aloe Vera Barbadensis Miller Leaf Extract Effects On Glutamate and Glyphosate Induced Toxicity: In Vitro Study. , 2021, ss.376 - 381. 10.35229/jaes.953830 |
| AMA | Yilmaz A,TAGHİZADEHGHALEHJOUGHİ A,Hacimuftuoglu A,Türkmen A Investigation of Aloe Vera Barbadensis Miller Leaf Extract Effects On Glutamate and Glyphosate Induced Toxicity: In Vitro Study. . 2021; 376 - 381. 10.35229/jaes.953830 |
| Vancouver | Yilmaz A,TAGHİZADEHGHALEHJOUGHİ A,Hacimuftuoglu A,Türkmen A Investigation of Aloe Vera Barbadensis Miller Leaf Extract Effects On Glutamate and Glyphosate Induced Toxicity: In Vitro Study. . 2021; 376 - 381. 10.35229/jaes.953830 |
| IEEE | Yilmaz A,TAGHİZADEHGHALEHJOUGHİ A,Hacimuftuoglu A,Türkmen A "Investigation of Aloe Vera Barbadensis Miller Leaf Extract Effects On Glutamate and Glyphosate Induced Toxicity: In Vitro Study." , ss.376 - 381, 2021. 10.35229/jaes.953830 |
| ISNAD | Yilmaz, Aysegul vd. "Investigation of Aloe Vera Barbadensis Miller Leaf Extract Effects On Glutamate and Glyphosate Induced Toxicity: In Vitro Study". (2021), 376-381. https://doi.org/10.35229/jaes.953830 |
| APA | Yilmaz A, TAGHİZADEHGHALEHJOUGHİ A, Hacimuftuoglu A, Türkmen A (2021). Investigation of Aloe Vera Barbadensis Miller Leaf Extract Effects On Glutamate and Glyphosate Induced Toxicity: In Vitro Study. JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES, 6(3), 376 - 381. 10.35229/jaes.953830 |
| Chicago | Yilmaz Aysegul,TAGHİZADEHGHALEHJOUGHİ Ali,Hacimuftuoglu Ahmet,Türkmen Aybike Investigation of Aloe Vera Barbadensis Miller Leaf Extract Effects On Glutamate and Glyphosate Induced Toxicity: In Vitro Study. JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES 6, no.3 (2021): 376 - 381. 10.35229/jaes.953830 |
| MLA | Yilmaz Aysegul,TAGHİZADEHGHALEHJOUGHİ Ali,Hacimuftuoglu Ahmet,Türkmen Aybike Investigation of Aloe Vera Barbadensis Miller Leaf Extract Effects On Glutamate and Glyphosate Induced Toxicity: In Vitro Study. JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES, vol.6, no.3, 2021, ss.376 - 381. 10.35229/jaes.953830 |
| AMA | Yilmaz A,TAGHİZADEHGHALEHJOUGHİ A,Hacimuftuoglu A,Türkmen A Investigation of Aloe Vera Barbadensis Miller Leaf Extract Effects On Glutamate and Glyphosate Induced Toxicity: In Vitro Study. JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES. 2021; 6(3): 376 - 381. 10.35229/jaes.953830 |
| Vancouver | Yilmaz A,TAGHİZADEHGHALEHJOUGHİ A,Hacimuftuoglu A,Türkmen A Investigation of Aloe Vera Barbadensis Miller Leaf Extract Effects On Glutamate and Glyphosate Induced Toxicity: In Vitro Study. JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES. 2021; 6(3): 376 - 381. 10.35229/jaes.953830 |
| IEEE | Yilmaz A,TAGHİZADEHGHALEHJOUGHİ A,Hacimuftuoglu A,Türkmen A "Investigation of Aloe Vera Barbadensis Miller Leaf Extract Effects On Glutamate and Glyphosate Induced Toxicity: In Vitro Study." JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES, 6, ss.376 - 381, 2021. 10.35229/jaes.953830 |
| ISNAD | Yilmaz, Aysegul vd. "Investigation of Aloe Vera Barbadensis Miller Leaf Extract Effects On Glutamate and Glyphosate Induced Toxicity: In Vitro Study". JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES 6/3 (2021), 376-381. https://doi.org/10.35229/jaes.953830 |
