OBJECTIVES: Healthcare workers (HCWs) possess a high risk for both latent tuberculosis infection (LTBI) and active disease. This studyaimed to investigate tuberculosis (TB) disease history in hospital staff working in healthcare institutions in the Eastern Black Sea Regionof Turkey.MATERIAL AND METHODS: This study included 460 HCWs employed in 5 hospitals in the Eastern Black Sea Region of Turkey. BetweenMay 01 and July 31, 2016, the participants were asked to answer a questionnaire, including data about TB history. The data about familyTB history, Bacilli Calmette–Guerin (BCG) vaccination, or tuberculin skin test (TST) application before starting work were also evaluated. RESULTS: Of the 460 participants, 69.3% were women (n=319) and 30.7% (n=141) were men. The mean age was 32 (17–63) years. Atotal of 8 participants (1.7 %) had TB history. There was no statistically significant relationship between TB history and age, sex, bodymass index (BMI), smoking habits, or presence of comorbidities (p>0.05 for all variables); family history of TB (p<0.001) and TST positiv ity (p<0.001) were significantly higher in participants with a TB history. Each participant was checked for the presence of both BCG scarand TST positivity. No difference was noted between positive TB history and negative HCWs (p>0.05). The duration of work (years) washigher in participants with a TB history. The p value was very close but did not reach the limits of significance (p=0.059).CONCLUSION: In this study, the rate of TB among HCWs was 1.7% (8 of 460 HCWs). Family history of TB and TST positivity are strongpredictors of TB in HCWs.