Antibacterial and Anticancer Activities of Violacein Extracted Through Ultrasound-Assisted Extraction Method

şekerler, turgut; PERİZ, Çağdaş Deniz; Tinaz, Gulgun; Ulusoy, Seyhan

Antibacterial and Anticancer Activities of Violacein Extracted Through Ultrasound-Assisted Extraction Method

Çağdaş Deniz Periz, (Süleyman Demirel Üniversitesi, Fen-Edebiyat Fakültesi, Biyoloji Bölümü, Isparta, Türkiye)

Seyhan Ulusoy, (Süleyman Demirel Üniversitesi, Fen-Edebiyat Fakültesi, Biyoloji Bölümü, Isparta, Türkiye)

Gülgün Tınaz, (Marmara Üniversitesi, Eczacılık Fakültesi, Temel Eczacılık Bilimleri Anabilim Dalı, İstanbul, Türkiye)

Turgut Şekerler (Marmara Üniversitesi, Eczacılık Fakültesi, Biyokimya Anabilim Dalı, İstanbul, Türkiye)

Akademik Gıda

19 3

Yıl: 2020 Cilt: 18 Sayı: 3 Sayfa Aralığı: 241 - 246 Metin Dili: İngilizce İndeks Tarihi: 15-11-2021

Antibacterial and Anticancer Activities of Violacein Extracted Through Ultrasound-Assisted Extraction Method

Öz:

Violacein is a natural violet pigment produced by various bacterial species, especially by the Gram-negative bacteriaChromobacterium violaceum. Violacein has antibacterial, antifungal, antioxidant and anticancer activities and hasvarious applications in food, cosmetic, textile and pharmaceutical industries. In this study, violacein was extractedfrom the Chromobacterium violaceum culture through ultrasonic-assisted (UA) and microwave-assisted (MA)extraction methods using methanol, ethanol, acetone, and ethyl acetate as solvent. Violacein was quantified usingHigh Performance Liquid Chromatography (HPLC). Then, antibacterial and anticancer activities of these extracts wereinvestigated. In this study, for the first time, the violacein extract was obtained by using UA and MA extractionmethods. The highest violacein concentration could be achieved by UAE method with methanol. It was found out thatviolacein extracts had an antibacterial effect against Staphylococcus aureus, S. aureus (MRSA) and Bacillus cereus.The violacein extract had a strong anticancer effect on cervical cancer (HeLa) cells. Finally, the UA and MA extractionmethods were successfully applied to bacterial cultures in order to extract the violacein.

Anahtar Kelime:

Ultrason Destekli Ekstraksiyon Yöntemi ile Elde Edilen Violasinin Antibakteriyel ve Antikanser Aktiviteleri

Öz:

Viyolasin, özellikle Gram-negatif bakteri Chromobacterium violaceum tarafından üretilen ve antibakteriyel, antifungal, antioksidan ve antikanser etkilere sahip çeşitli bakteri türleri tarafından üretilen doğal mor bir pigmenttir. Bu çalışmada, viyolasin, Ultrason destekli (UA) ve mikrodalga destekli (MA) ekstraksiyon prosedürleri ile C. violaceum suşundan metanol, etanol, aseton ve etil asetat kullanılarak ekstrakte edilmiştir. Elde edilen viyolasin miktarı, yüksek performanslı sıvı kromatografisi (HPLC) kullanılarak belirlenmiştir. Daha sonra bu ekstraktların antibakteriyel ve antikanser aktiviteleri incelenmiştir. Bu çalışmada, viyolasin ilk kez UA ve MA ekstraksiyon yöntemleri kullanılarak elde edilmiştir. Viyolasin ekstrelerinin Staphylococcus aureus, S. aureus (MRSA) ve Bacillus cereus'a karşı antibakteriyel aktiviteye sahip olduğu belirlenmiştir. Viyolasin ekstresi rahim ağzı kanseri hücreleri (HeLa) üzerinde güçlü anti-kanser etkisi göstermiştir. Sonuçta, viyolasini ekstrakte etmek için, UA ve MA ekstraksiyon yöntemleri bakteri kültürlerine başarıyla uygulanmıştır.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	PERİZ Ç, Ulusoy S, Tinaz G, şekerler t (2020). Antibacterial and Anticancer Activities of Violacein Extracted Through Ultrasound-Assisted Extraction Method. , 241 - 246.
Chicago	PERİZ Çağdaş Deniz,Ulusoy Seyhan,Tinaz Gulgun,şekerler turgut Antibacterial and Anticancer Activities of Violacein Extracted Through Ultrasound-Assisted Extraction Method. (2020): 241 - 246.
MLA	PERİZ Çağdaş Deniz,Ulusoy Seyhan,Tinaz Gulgun,şekerler turgut Antibacterial and Anticancer Activities of Violacein Extracted Through Ultrasound-Assisted Extraction Method. , 2020, ss.241 - 246.
AMA	PERİZ Ç,Ulusoy S,Tinaz G,şekerler t Antibacterial and Anticancer Activities of Violacein Extracted Through Ultrasound-Assisted Extraction Method. . 2020; 241 - 246.
Vancouver	PERİZ Ç,Ulusoy S,Tinaz G,şekerler t Antibacterial and Anticancer Activities of Violacein Extracted Through Ultrasound-Assisted Extraction Method. . 2020; 241 - 246.
IEEE	PERİZ Ç,Ulusoy S,Tinaz G,şekerler t "Antibacterial and Anticancer Activities of Violacein Extracted Through Ultrasound-Assisted Extraction Method." , ss.241 - 246, 2020.
ISNAD	PERİZ, Çağdaş Deniz vd. "Antibacterial and Anticancer Activities of Violacein Extracted Through Ultrasound-Assisted Extraction Method". (2020), 241-246.

APA	PERİZ Ç, Ulusoy S, Tinaz G, şekerler t (2020). Antibacterial and Anticancer Activities of Violacein Extracted Through Ultrasound-Assisted Extraction Method. Akademik Gıda, 18(3), 241 - 246.
Chicago	PERİZ Çağdaş Deniz,Ulusoy Seyhan,Tinaz Gulgun,şekerler turgut Antibacterial and Anticancer Activities of Violacein Extracted Through Ultrasound-Assisted Extraction Method. Akademik Gıda 18, no.3 (2020): 241 - 246.
MLA	PERİZ Çağdaş Deniz,Ulusoy Seyhan,Tinaz Gulgun,şekerler turgut Antibacterial and Anticancer Activities of Violacein Extracted Through Ultrasound-Assisted Extraction Method. Akademik Gıda, vol.18, no.3, 2020, ss.241 - 246.
AMA	PERİZ Ç,Ulusoy S,Tinaz G,şekerler t Antibacterial and Anticancer Activities of Violacein Extracted Through Ultrasound-Assisted Extraction Method. Akademik Gıda. 2020; 18(3): 241 - 246.
Vancouver	PERİZ Ç,Ulusoy S,Tinaz G,şekerler t Antibacterial and Anticancer Activities of Violacein Extracted Through Ultrasound-Assisted Extraction Method. Akademik Gıda. 2020; 18(3): 241 - 246.
IEEE	PERİZ Ç,Ulusoy S,Tinaz G,şekerler t "Antibacterial and Anticancer Activities of Violacein Extracted Through Ultrasound-Assisted Extraction Method." Akademik Gıda, 18, ss.241 - 246, 2020.
ISNAD	PERİZ, Çağdaş Deniz vd. "Antibacterial and Anticancer Activities of Violacein Extracted Through Ultrasound-Assisted Extraction Method". Akademik Gıda 18/3 (2020), 241-246.