Yıl: 2021 Cilt: 10 Sayı: 2 Sayfa Aralığı: 450 - 454 Metin Dili: İngilizce İndeks Tarihi: 10-02-2022

Effects of storage conditions on thiol disulfide homeostasis

Öz:
We planned this study to research the effect of freeze-thaw number and storage temperatures on the tests of thiol-disulfide homeostasis. Dynamic thiol-disulfide homeostasis has a crucial role in antioxidant defense reactions. Thiol disulfide homeostasis is detected in serum samples. To gauge the steadiness at different storage temperatures, sera stored at -20°C and -80°C for 1, 2, 3, 6, and 12 months. To work out the effect of the freeze-thaw cycle, sera were frozen at -80°C and subjected to ten freeze-thaw cycles at 24-hour intervals. According to our results, native thiol, total thiol, and disulfide levels in human serum samples were stable at -20°C for three months and at -80°C for 12 months. After the third month at -20°C, native thiol and total thiol levels decreased while disulfide levels increased. Additionally, as the number of freeze-thaw cycles increased, native thiol and total thiol levels decreased, and disulfide levels increased. Our results show that storage at -20°C and an increasing number of freezethaw processes affect thiols levels. To get the most accurate results, repeated freeze-thaw cycles should be avoided the maximum amount possible. Also, samples should be frozen at -80°C if long storage times are required.
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APA BALIK A, Özbek B, Yilmaz G, KOZACI L, EREL O, koca c (2021). Effects of storage conditions on thiol disulfide homeostasis. , 450 - 454.
Chicago BALIK Ahmet Rıfat,Özbek Betül,Yilmaz Gulsen,KOZACI LEYLA DIDEM,EREL Ozcan,koca cemil Effects of storage conditions on thiol disulfide homeostasis. (2021): 450 - 454.
MLA BALIK Ahmet Rıfat,Özbek Betül,Yilmaz Gulsen,KOZACI LEYLA DIDEM,EREL Ozcan,koca cemil Effects of storage conditions on thiol disulfide homeostasis. , 2021, ss.450 - 454.
AMA BALIK A,Özbek B,Yilmaz G,KOZACI L,EREL O,koca c Effects of storage conditions on thiol disulfide homeostasis. . 2021; 450 - 454.
Vancouver BALIK A,Özbek B,Yilmaz G,KOZACI L,EREL O,koca c Effects of storage conditions on thiol disulfide homeostasis. . 2021; 450 - 454.
IEEE BALIK A,Özbek B,Yilmaz G,KOZACI L,EREL O,koca c "Effects of storage conditions on thiol disulfide homeostasis." , ss.450 - 454, 2021.
ISNAD BALIK, Ahmet Rıfat vd. "Effects of storage conditions on thiol disulfide homeostasis". (2021), 450-454.
APA BALIK A, Özbek B, Yilmaz G, KOZACI L, EREL O, koca c (2021). Effects of storage conditions on thiol disulfide homeostasis. Medicine Science, 10(2), 450 - 454.
Chicago BALIK Ahmet Rıfat,Özbek Betül,Yilmaz Gulsen,KOZACI LEYLA DIDEM,EREL Ozcan,koca cemil Effects of storage conditions on thiol disulfide homeostasis. Medicine Science 10, no.2 (2021): 450 - 454.
MLA BALIK Ahmet Rıfat,Özbek Betül,Yilmaz Gulsen,KOZACI LEYLA DIDEM,EREL Ozcan,koca cemil Effects of storage conditions on thiol disulfide homeostasis. Medicine Science, vol.10, no.2, 2021, ss.450 - 454.
AMA BALIK A,Özbek B,Yilmaz G,KOZACI L,EREL O,koca c Effects of storage conditions on thiol disulfide homeostasis. Medicine Science. 2021; 10(2): 450 - 454.
Vancouver BALIK A,Özbek B,Yilmaz G,KOZACI L,EREL O,koca c Effects of storage conditions on thiol disulfide homeostasis. Medicine Science. 2021; 10(2): 450 - 454.
IEEE BALIK A,Özbek B,Yilmaz G,KOZACI L,EREL O,koca c "Effects of storage conditions on thiol disulfide homeostasis." Medicine Science, 10, ss.450 - 454, 2021.
ISNAD BALIK, Ahmet Rıfat vd. "Effects of storage conditions on thiol disulfide homeostasis". Medicine Science 10/2 (2021), 450-454.