The short-term effects of single toxic dose of citric acid in mice

BAKAR, Elvan; AKTAÇ, Tülin; KARAKAŞ, Hamiyet; KABOĞLU, Ayşegül

The short-term effects of single toxic dose of citric acid in mice

Tülin AKTAÇ, (Trakya Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Edirne, Türkiye)

Ayşegül KABOĞLU, (Trakya Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Edirne, Türkiye)

Elvan BAKAR, (Trakya Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Edirne, Türkiye)

Hamiyet KARAKAŞ (Trakya Üniversitesi, Tıp Fakültesi, Biyokimya Bölümü, Edirne, Türkiye)

Journal of Cell and Molecular Biology

1 0

Yıl: 2003 Cilt: 2 Sayı: 1 Sayfa Aralığı: 19 - 23 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

The short-term effects of single toxic dose of citric acid in mice

Öz:

Bir besin koruyucu olan sitrik asidin LD25 (480 mg/kg.va.) dozu farelere intraperitoneal yolla uygulandı. 10 gün sonra hayvanların vücut ağırlıkları, organ ağırlıkları (karaciğer, böbrek, dalak), kreatin kinaz (CK), laktat dehidrogenaz (LDH), alanin aminotransferaz (ALT) ve aspartat aminotransferaz (AST) enzimlerinin serum düzeyleri ile, karaciğer dokusu üzerinde sitrik asidin etkileri araştırıldı. Otoanalizörde tayin edilen enzim aktiviteleri U/L olarak değerlendirildi. Çalışmada vücut ağırlıklarında kontrol grubuna kıyasla anlamlı bir azalma gözlenmesine rağmen (p<0.001), organ ağırlıklarında (karaciğer: p>0.05 , böbrek: p>0.05, dalak: p>0.05) ve enzim aktivitelerinde (CK: p>0.05, LDH: p>0.05, ALT: p>0.05, AST: p>0.05) anlamlı olmayan bir artış gözlendi. Karaciğerin mikroskopik incelenmesinde doku dejenerasyonu, sitoplazmik vakuolizasyon, nükleer zar çöküntüleri, piknotik nukleuslar ve hepatositlerde nekroz gibi histopatolojik değişiklikler gözlendi.

Anahtar Kelime: fareler toksik maddeler vücut ağırlığı histopatoloji enzim aktivitesi toksisite gıda koruyucuları sitrik asit

Konular: Biyokimya ve Moleküler Biyoloji

Farelerde sitrik asidin tek toksik dozunun kısa süreli etkileri

Öz:

The effects of LD25 (480 mg/kg.bw.) dose of citric acid, a food preservative, were investigated on body weight, organ weights (liver, kidney, spleen), creatin kinase (CK), lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) enzymes in the blood serum, and the liver tissue of mice after 10 days. Citric acid (to experimental groups) and physiological saline (to control groups) were given intraperitoneally. The results of enzyme activities were evaluated using autoanalyzer as IU/L. Even though significant decreases in the body weights were noted when compared to those of the control group (p<0.001), generally an insignificant increase were observed in the organ weights (liver: p>0.05, kidney: p>0.05, spleen: p>0.05) and serum enzyme levels (CK: p>0.05, LDH,: p>0.05, ALT: p>0.05, AST: p>0.05). Microscopical examination of the liver showed histopathological changes depending on the citric acid. These changes were tissue degeneration, cytoplasmic vacuolisations, nuclear membrane invaginations, picnotic nucleus and necrosis of the hepatocytes.

Anahtar Kelime: histopathology enzyme activity toxicity food preservatives citric acid mice toxic substances body weight

Konular: Biyokimya ve Moleküler Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	AKTAÇ T, KABOĞLU A, BAKAR E, KARAKAŞ H (2003). The short-term effects of single toxic dose of citric acid in mice. , 19 - 23.
Chicago	AKTAÇ Tülin,KABOĞLU Ayşegül,BAKAR Elvan,KARAKAŞ Hamiyet The short-term effects of single toxic dose of citric acid in mice. (2003): 19 - 23.
MLA	AKTAÇ Tülin,KABOĞLU Ayşegül,BAKAR Elvan,KARAKAŞ Hamiyet The short-term effects of single toxic dose of citric acid in mice. , 2003, ss.19 - 23.
AMA	AKTAÇ T,KABOĞLU A,BAKAR E,KARAKAŞ H The short-term effects of single toxic dose of citric acid in mice. . 2003; 19 - 23.
Vancouver	AKTAÇ T,KABOĞLU A,BAKAR E,KARAKAŞ H The short-term effects of single toxic dose of citric acid in mice. . 2003; 19 - 23.
IEEE	AKTAÇ T,KABOĞLU A,BAKAR E,KARAKAŞ H "The short-term effects of single toxic dose of citric acid in mice." , ss.19 - 23, 2003.
ISNAD	AKTAÇ, Tülin vd. "The short-term effects of single toxic dose of citric acid in mice". (2003), 19-23.

APA	AKTAÇ T, KABOĞLU A, BAKAR E, KARAKAŞ H (2003). The short-term effects of single toxic dose of citric acid in mice. Journal of Cell and Molecular Biology, 2(1), 19 - 23.
Chicago	AKTAÇ Tülin,KABOĞLU Ayşegül,BAKAR Elvan,KARAKAŞ Hamiyet The short-term effects of single toxic dose of citric acid in mice. Journal of Cell and Molecular Biology 2, no.1 (2003): 19 - 23.
MLA	AKTAÇ Tülin,KABOĞLU Ayşegül,BAKAR Elvan,KARAKAŞ Hamiyet The short-term effects of single toxic dose of citric acid in mice. Journal of Cell and Molecular Biology, vol.2, no.1, 2003, ss.19 - 23.
AMA	AKTAÇ T,KABOĞLU A,BAKAR E,KARAKAŞ H The short-term effects of single toxic dose of citric acid in mice. Journal of Cell and Molecular Biology. 2003; 2(1): 19 - 23.
Vancouver	AKTAÇ T,KABOĞLU A,BAKAR E,KARAKAŞ H The short-term effects of single toxic dose of citric acid in mice. Journal of Cell and Molecular Biology. 2003; 2(1): 19 - 23.
IEEE	AKTAÇ T,KABOĞLU A,BAKAR E,KARAKAŞ H "The short-term effects of single toxic dose of citric acid in mice." Journal of Cell and Molecular Biology, 2, ss.19 - 23, 2003.
ISNAD	AKTAÇ, Tülin vd. "The short-term effects of single toxic dose of citric acid in mice". Journal of Cell and Molecular Biology 2/1 (2003), 19-23.