The Efficacy of Hydroalcoholic Extracts of Prosopis farcta Against Leishmania major

Riahi, Seyed Mohammad; Namaei, Mohammad Hasan; solgi, rahmatollah; Hatam, Gholamreza; tasa, davood; Karamian, Mehdi; Rajaii, Toktam; Shafaie, Ebrahim

doi:10.4274/tpd.galenos.2020.6956

The Efficacy of Hydroalcoholic Extracts of Prosopis farcta Against Leishmania major

Mohammad Hasan NAMAEİ, (Birjand University of Medical Sciences, Department of Medical Microbiology, Infectious Disease Research Center, Birjand, İran)

Rahmat SOLGİ, (Birjand University of Medical Sciences, Department of Medical Microbiology, Infectious Disease Research Center, Birjand, İran)

Toktem RAJAİİ, (Mashhad University of Medical Sciences, Health Center, Mashhad, İran)

Ebrahim SHAFAİE, (1 Birjand University of Medical Sciences, Department of Medical Microbiology, Infectious Disease Research Center, Birjand, İran)

Mehdi KARAMİAN, (Zanjan University of Medical Sciences, Department of Medical Parasitology and Mycology, Zanjan, İran)

Gholamreza HATAM, (Basic Sciences in Infectious Diseases Research Centre, Shiraz University of Medical Sciences, Shiraz, İran)

Davoud TASA, (Tehran University of Medical Sciences, Department of General Surgery, Tehran, İran)

Seyed Mohammad RİAHİ (Birjand University of Medical Sciences, Department of Public Health, Birjand, İran)

Türkiye Parazitoloji Dergisi

2 0

Yıl: 2021 Cilt: 45 Sayı: 1 Sayfa Aralığı: 24 - 27 Metin Dili: İngilizce DOI: 10.4274/tpd.galenos.2020.6956 İndeks Tarihi: 14-01-2022

The Efficacy of Hydroalcoholic Extracts of Prosopis farcta Against Leishmania major

Öz:

Objective: Cutaneous leishmaniasis caused by Leishmania major (L. major) is an endemic disease in Iran. The current reference drugs, including Glucantime, possess high toxicity in addition to some side-effects. Therefore, there is a growing interest in exploring biomedical plants. The goal of the present study was to evaluate the anti-leishmanial activity and cytotoxicity of hydroalcoholic extracts from Prosopis farcta (P. farcta) over promastigote and amastigote forms. Methods: This study was performed at the Iran Birjand University of Medical Sciences, during the year 2019. In this study, the hydroalcoholic extracts of the stems, leaves (LE) and fruits (FE) of P. farcta were obtained. The anti-leishmanial activity was assessed against leptomonad promastigotes and intracellular amastigotes of L. major. The cytotoxicity of these extracts was determined in murine macrophages. Results: The FE and LE of P. farcta demonstrated a significant leishmanicidal effect against L. major promastigotes with an IC50 of 0.9 mg/mL and 1.1 mg/mL, respectively. The FE showed the most anti-leishmanial activity and presented with the highest index of selectivity (SI=14.6) as an anti-leishmanial product. Infected macrophages treated using the FE showed a reduction in parasite burden by 97.3%. Conclusion: The results of the present study demonstrated the leishmanicidal activity of P. farcta on both promastigotes and intracellular amastigotes. There is a need for performing comprehensive studies on relevant animal models and to access the effects of active components of P. farcta extract on the growth of L. major.

Anahtar Kelime:

Prosopis farcta’nın Hidroalkolik Ekstraktının Leishmania major'a Karşı Etkinliği

Öz:

Amaç: Leishmania major'un (L. major) neden olduğu kutanöz leishmaniasis, İran’da endemik bir hastalıktır. Glucantime dahil olmak üzere mevcut referans ilacın yüksek bir toksisitesi ve yan etkisi vardır. Bu nedenle, biyomedikal bitkilere ilgi artmıştır. Çalışmanın amacı, sitotoksisitenin yanı sıra promastigot ve amastigot formları üzerindeki anti-Leishmania aktivitesinin değerlendirilmesidir. Yöntemler: Bu çalışma, 2019 yılında İran’ın Birjand Tıp Bilimleri Üniversitesi’nde yapılmıştır. Bu çalışmada Prosopis farcta’nın (P. farcta) saplarının, yapraklarının (LE) ve meyvelerinin (FE) hidroalkolik özleri elde edilmiştir. Anti-leishmanial aktivite, leptomonad promastigotlara ve L. major'un hücre içi amastigotlarına karşı değerlendirilmiştir. Bu ekstraktların sitotoksisitesi fare makrofajlarında belirlenmiştir. Bulgular: P. farcta’nın FE ve LE değerleri, sırasıyla 0,9 mg/mL ve 1,1 mg/mL IC50 ile L. major promastigotlara karşı anlamlı bir leishmanisidal etki göstermiştir. Meyve ekstraktı en yüksek anti-leishmanial aktiviteye sahip olup, bir anti-leishmanial ürün olarak en yüksek seçicilik endeksini (SI=14,6) sunmuştur. FE kullanılarak, enfekte makrofajlarda tedavi etkinliği de ölçülmüş ve parazit yükünde %97,3 oranında bir azalma elde edilmiştir. Sonuç: Bu çalışmanın sonuçları, P. farcta’nın hem promastigotlar hem de hücre içi amastigotlar üzerindeki leishmanisidal aktivitesini göstermiştir. L. major'a karşı kesin etkisini değerlendirmek için hayvan modeli ve P. farcta ekstresinin aktif bileşeni üzerinde kapsamlı çalışmalara ihtiyaç bulunmaktadır.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Namaei M, solgi r, Rajaii T, Shafaie E, Karamian M, Hatam G, tasa d, Riahi S (2021). The Efficacy of Hydroalcoholic Extracts of Prosopis farcta Against Leishmania major. , 24 - 27. 10.4274/tpd.galenos.2020.6956
Chicago	Namaei Mohammad Hasan,solgi rahmatollah,Rajaii Toktam,Shafaie Ebrahim,Karamian Mehdi,Hatam Gholamreza,tasa davood,Riahi Seyed Mohammad The Efficacy of Hydroalcoholic Extracts of Prosopis farcta Against Leishmania major. (2021): 24 - 27. 10.4274/tpd.galenos.2020.6956
MLA	Namaei Mohammad Hasan,solgi rahmatollah,Rajaii Toktam,Shafaie Ebrahim,Karamian Mehdi,Hatam Gholamreza,tasa davood,Riahi Seyed Mohammad The Efficacy of Hydroalcoholic Extracts of Prosopis farcta Against Leishmania major. , 2021, ss.24 - 27. 10.4274/tpd.galenos.2020.6956
AMA	Namaei M,solgi r,Rajaii T,Shafaie E,Karamian M,Hatam G,tasa d,Riahi S The Efficacy of Hydroalcoholic Extracts of Prosopis farcta Against Leishmania major. . 2021; 24 - 27. 10.4274/tpd.galenos.2020.6956
Vancouver	Namaei M,solgi r,Rajaii T,Shafaie E,Karamian M,Hatam G,tasa d,Riahi S The Efficacy of Hydroalcoholic Extracts of Prosopis farcta Against Leishmania major. . 2021; 24 - 27. 10.4274/tpd.galenos.2020.6956
IEEE	Namaei M,solgi r,Rajaii T,Shafaie E,Karamian M,Hatam G,tasa d,Riahi S "The Efficacy of Hydroalcoholic Extracts of Prosopis farcta Against Leishmania major." , ss.24 - 27, 2021. 10.4274/tpd.galenos.2020.6956
ISNAD	Namaei, Mohammad Hasan vd. "The Efficacy of Hydroalcoholic Extracts of Prosopis farcta Against Leishmania major". (2021), 24-27. https://doi.org/10.4274/tpd.galenos.2020.6956

APA	Namaei M, solgi r, Rajaii T, Shafaie E, Karamian M, Hatam G, tasa d, Riahi S (2021). The Efficacy of Hydroalcoholic Extracts of Prosopis farcta Against Leishmania major. Türkiye Parazitoloji Dergisi, 45(1), 24 - 27. 10.4274/tpd.galenos.2020.6956
Chicago	Namaei Mohammad Hasan,solgi rahmatollah,Rajaii Toktam,Shafaie Ebrahim,Karamian Mehdi,Hatam Gholamreza,tasa davood,Riahi Seyed Mohammad The Efficacy of Hydroalcoholic Extracts of Prosopis farcta Against Leishmania major. Türkiye Parazitoloji Dergisi 45, no.1 (2021): 24 - 27. 10.4274/tpd.galenos.2020.6956
MLA	Namaei Mohammad Hasan,solgi rahmatollah,Rajaii Toktam,Shafaie Ebrahim,Karamian Mehdi,Hatam Gholamreza,tasa davood,Riahi Seyed Mohammad The Efficacy of Hydroalcoholic Extracts of Prosopis farcta Against Leishmania major. Türkiye Parazitoloji Dergisi, vol.45, no.1, 2021, ss.24 - 27. 10.4274/tpd.galenos.2020.6956
AMA	Namaei M,solgi r,Rajaii T,Shafaie E,Karamian M,Hatam G,tasa d,Riahi S The Efficacy of Hydroalcoholic Extracts of Prosopis farcta Against Leishmania major. Türkiye Parazitoloji Dergisi. 2021; 45(1): 24 - 27. 10.4274/tpd.galenos.2020.6956
Vancouver	Namaei M,solgi r,Rajaii T,Shafaie E,Karamian M,Hatam G,tasa d,Riahi S The Efficacy of Hydroalcoholic Extracts of Prosopis farcta Against Leishmania major. Türkiye Parazitoloji Dergisi. 2021; 45(1): 24 - 27. 10.4274/tpd.galenos.2020.6956
IEEE	Namaei M,solgi r,Rajaii T,Shafaie E,Karamian M,Hatam G,tasa d,Riahi S "The Efficacy of Hydroalcoholic Extracts of Prosopis farcta Against Leishmania major." Türkiye Parazitoloji Dergisi, 45, ss.24 - 27, 2021. 10.4274/tpd.galenos.2020.6956
ISNAD	Namaei, Mohammad Hasan vd. "The Efficacy of Hydroalcoholic Extracts of Prosopis farcta Against Leishmania major". Türkiye Parazitoloji Dergisi 45/1 (2021), 24-27. https://doi.org/10.4274/tpd.galenos.2020.6956