Objective: This study aimed to evaluate caregiver burden among caregivers of acute stroke patients with a biopsychosocial perspective in a Turkey sample. Methods: 72 stroke patients and 72 their caregivers were included the study. The mean age of the stroke patients included in the study was 65±12.39. The mean age of caregivers was 44.5±14 and 66.7% of them were females. Modified Motor Assessment Scale (MMAS), Standardized Mini Mental State Examination (SMMSE) and The Barthel Index (BI) were used to assess the patients with stroke. The caregivers were evaluated by using the Bakas Caregiving Outcomes Scale, Family Sense of Coherence Scale-Short Form (FSOC-S), Hospital Anxiety and Depression Scale (HADS), WHOQOL-Bref-Short Form and Multidimensional Scale of Perceived Social Support (MSPSS). Results: There were significant positive correlation between the BCOS score and the SMMSE (r=0.36; p=0.002) and BI (r=0.22; p=0.05) scores. A significant positive correlation was found between the BCOS score and MSPSS’s family (r=0.31; p=0.007), friend (r=0.41; p<0.01) and special human (r=0.46; p<0.01) sub-parameters. In addition, there were significant positive correlations between BCOS score and the physical (r=0.35; p=0.02) and environmental (r=0.42; p<0.01) sub-dimensions of the WHOQOL-BREF, also HADS Depression sub-score (r=0.93; p=0.01). Correlations between BCOS score and patients' age, MMAS, FSOC-S, scores and HAD Anxiety sub-score were not statistically significant (p>0.05). Conclusion: The cognitive function and independence level of the patients is associated with care burden. Furthermore, psychosocial features such as poor social functioning, quality of life and emotional health of caregiver have adverse effects on caregiver burden.