Yıl: 2021 Cilt: 47 Sayı: 3 Sayfa Aralığı: 179 - 187 Metin Dili: İngilizce DOI: 10.5152/actavet.2021.20057 İndeks Tarihi: 19-01-2022

Pathological Changes Associated with Aeromonas hydrophila Dose-Dependent Infection and Heat Stress in Red Hybrid Tilapia

Öz:
Aeromonas hydrophila constitutes a normal microflora of fish. Heat stress in red hybrid tilapia may induce higher mortality if the fish are infected with Aeromonas. In this study, 192 red tilapia fish were divided into eight groups, A–H, each consisting of 24 red hybrid tilapia. Groups A, B, and C were inoculated with 0.1 mL of 1012 CFU/mL, 1010 CFU/mL, and 108CFU/mL of A. hydrophila respectively, while group D was inoculated with 0.1 mL of phosphate-buffered saline (as control for the heat-stressed groups); and heat stress was introduced from day 8 through the day 14 post-infection (pi). Groups E, F, and G were infected with similar doses as in A, B, and C respectively, with no heat stress, while Group H served as negative control for non-heat stress. Mortality was higher in the heat-stressed groups, at 44.47%, as against 16.7% for the non-heat-stressed groups. The clinical signs included hemorrhages on the abdomen, mouth, and dorsal fin, and abdominal distension most predominantly in the heat-stressed groups. Gross lesions included enlargement of the kidney and the spleen and yellowish ascitic fluid, the histopathological lesions included pyknosis and desquamation of the renal tubular epithelium, congestion, necrosis, and fatty changes in the liver, while the spleen showed congestion of the splenic blood vessels. The lesions were seen predominantly in the heat-stressed groups, most severely in group A. The effect of the different doses was more significant in group A compared to the other groups. This study revealed that heat stress predisposes red hybrid tilapia infected with A. hydrophilia to increased disease severity and higher mortality, with the degree of initial infection playing a significant role.
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  • Afifi, S. H., Al-Thobiati, S., & Hazaa, M. S. (2000). Bacteriological and histopathological studies on Aeromonas hydrophila infection of Nile tilapia (Oreochromis niloticus) from fish farms in Saudi Arabia. Assiut Veterinary Medical Journal, 84, 195–205.
  • Ahmed, M., & El-Ashram, M. (2002). On Aeromonas hydrophilia infection among cultured tilapias: A biological, histopathological and management study. Egyptian Journal of Aquatic Biology and Fisheries, 6(3), 181–202.
  • Al-Haider, S. M., Al-Niaeem, K. S., & Resen, A. K. (2019). Prevalence and antibiotic resistance in Aeromonas species isolated from common carp (Cyprinus carpio L.) cultivated in floating cages at Al-Hilla river. Basrah Journal of Agricultural Sciences, 32(2), 33–43. [CrossRef]
  • Al-Yahya, S. A., Ameen, F., Al-Niaeem, K. S., Al-Sa’adi, B. A., Hadi, S., & Mostafa, A. A. (2018). Histopathological studies of experimental Aeromonas hydrophila infection in blue tilapia, Oreochromis aureus. Saudi Journal of Biological Sciences, 25(1), 182–185. [CrossRef]
  • Azad, I. S., Rajendran, K. V., Rajan, J. S., Vijayan, T. C., & Santiago, T. C. (2001). Virulence and histopathology of Aeromonas hydrophila (SAH 93) in experimentally infected tilapia, Oreochrornis mossambicus (L.). Journal of Aquaculture in the Tropics, 16(3), 265–275.
  • Chester, F. D. (1901). A Manual of Determinative Bacteriology. New York: Macmillan. p. 235.
  • Cipriano, C., Bullock, G. L., & Pyle, S. W. (2001). Aeromonas hydrophila and motile aeromonad septicemias of fish. Revision of Fish Disease Leaflet, 68 (1984).
  • El-Sayed, A. F. M. (2006). Tilapia culture. Cambrige, USA: CABI Publishing. Emikpe, B. O., Tanko, P. N., Onilude, O. M., & Sabri, M. Y. (2013). The influence of dexamethasone treatment and successive road transport stress on the occurrence of caprine pneumonia in a hot humid tropical environment. Veterinary World, 6(8), 497–501.
  • Faisal, M., Popp, W., & Refai, M. (1989). Aeromonas hydrophila-related septicemia in the Nile tilapia (Oreochromis niloticus). Berliner und Munchener Tierarztliche Wochenschrift, 102(3), 87–93.
  • González Serrano, C. J., Santos, J. A., García López, M. L., & Otero, A. (2002). Virulence markers in Aeromonas hydrophila and Aeromonas veroniibiovarsobria isolates from freshwater fish and from a diarrhoea case. Journal of Applied Microbiology, 93(3), 414–419. [CrossRef]
  • Groberg Jr, W. J., McCoy, R. H., Pilcher, K. S., & Fryer, J. L. (1978). Relation of water temperature to infections of coho salmon (Oncorhynchus kisutch), chinook salmon (O. tshawytscha), and steelhead trout (Salmogairdneri) with Aeromonas salmonicida and A. hydrophila. Journal of the Fisheries Board of Canada, 1–7.
  • Inglis, V., Roberts, R. S., & Bromage, R. (1993). Bacterial diseases of fish (1st ed, p. 312), New York: Halsted Press.
  • Isiaku, A. I., Sabri, M. Y., Ina-Salwany, M. Y., Hassan, M. D., Tanko, P. N., & Bello, M. B. (2017). Biofilm is associated with chronic streptococcal meningoencephalitis in fish. Microbial Pathogenesis, 102, 59–68. [CrossRef]
  • Lakshmana Rao, P. V., Gupta, N., & Jayaraj, R. (2004). Screening of certain chemoprotectants against cyclic peptide toxin microcystin-LR. Indian Journal of Pharmacology, 36(2), 87.
  • Lin, G., Wang, L., Te Ngoh, S. T., Ji, L., Orbán, L., & Yue, G. H. (2018). Mapping QTL for omega-3 content in hybrid saline tilapia. Marine Biotechnology, 20(1), 10–19. [CrossRef]
  • Linnaeus, C. (1958). Systema naturae per regna tria naturae, secundum classes, ordines, genera, species, cum characteribus, differentiis, synonymis. Tomus 1. L locis (Ed.), 10. Stockholm, Sweden: Salvii.
  • Maid, I., Mustafa, M. M., Arab, R. M. H., & Rezk, M. A. (2008). Prevalence of Aeromonas hydrophilia infection in wild and cultured tilapi anilotica (O. niloticus) in Egypt. Proceedings of 8th International symposium on tilapia in aquaculture, 12-14 October, Cairo, Egypt.
  • McFarland, J. (1907). The nephelometer: An instrument for estimating the number of bacteria in suspensions used for calculating the opsonic index and for vaccines. JAMA, 49(14), 1176–1178.
  • Nadirah, A. N., Sabri, M. Y., Emikpe, B. O., Noraini, O., Tanko, P. N., Opeyemi, O. M., & Ina-Salwany, M. Y. (2016). The effect of heat stress on the oxidative status of red hybrid tilapia (Oreochromis sp.) infected with. Streptococcus agalactiae. International Journal of Pharmaceutical Research and Allied Sciences, 5(4).
  • Noraini, O., Jahwarhar, N. A., Sabri, M. Y., Emikpe, B. O., Tanko, P. N., Latifah, M. H., & Jamil, S. (2013). The effect of heat stress on clinicopathological changes and immunolocalization of antigens in experimental Streptococcus agalactiae infection in Red hybrid tilapia (Oreochromis spp.). Veterinary World, 6(12), 997–1003. [CrossRef]
  • Polycarp, N. T., Mohd Yusoff, S. M., Emikpe, B. O., Siti-Khairani, B., & Shahrom, S. (2020). Resveratrol ameliorates pathophysiological changes associated with Brucella melitensis infection in dexamethasone-treated does. Comparative Clinical Pathology, 29(1), 255–266. [CrossRef]
  • Popma, T., & Masser, M. (1999). Tilapia life story and biology. Southern regional aquaculture center publication no. 283.
  • Roberts, R. J. (2012). Fish pathology (4th ed). Oxford: Blackwell Publishing Ltd.
  • Rodriguez, L. A., Ellis, A. E., & Nieto, T. P. (1992). Effects of acetylcholinesterase toxin of Aeromonas hydrophila on the central nervous system of fish. Microbial Pathogenesis, 14, 411–415. [CrossRef]
  • Shayo, S. D., Mwita, C. J., & Hosea, K. (2012). Ulcerative Aeromonas infections in tilapia (Cichlidae: Tilapiini) from Mtera hydropower dam, Tanzania, 1, 115. [CrossRef]
  • Shoemaker, C. A., Klesius, P. H., & Evans, J. J. (2000). Disease of tilapia with emphasis on economically important pathogens. 5th International symposium on tilapia aquaculture in 21st century, Brazil, 2, 565–572.
  • Skwor, T., Shinko, J., Augustyniak, A., Gee, C., & Andraso, G. (2014). Aeromonas hydrophila and Aeromonas veronii predominate among potentially pathogenic ciprofloxacin and tetracycline resistant Aeromonas isolates from Lake Erie. Applied and Environmental Microbiology, 80(3), 841–848. [CrossRef]
  • Tanko, P. N., Mohd Yusoff, S., Emikpe, B. O., Bejo, S. K., & Salisi, S. (2019). Effects of resveratrol on shedding and pathological dynamics in experimental B. melitensis infection in dexamethasone-treated nonpregnant boer goats. Journal of Immunoassay and Immunochemistry, 40(4), 419–438. [CrossRef]
  • Topić Popovic, N., Teskeredzić, E., Strunjak-Perović, I., & Coz-Rakovac, R. (2000). Aeromonas hydrophila isolated from wild freshwater fish in Croatia. Veterinary Research Communications, 24(6), 371–377. [CrossRef]
  • Wang, M., & Lu, M. (2016). Tilapia polyculture: A global review. Aquaculture Research, 47(8), 2363–2374. [CrossRef]
  • Yardımcı, B., & Aydın, Y. (2011). Pathological findings of experimental Aeromonas hydrophila infection in Nile tilapia (Oreochromis niloticus) (pp. 47–54). Ankara Üniversitesi Veteriner.
APA ALI N, M.Y S, TANKO NWUNUJI P, ABU NOR N, Onilude O (2021). Pathological Changes Associated with Aeromonas hydrophila Dose-Dependent Infection and Heat Stress in Red Hybrid Tilapia. , 179 - 187. 10.5152/actavet.2021.20057
Chicago ALI Nadiah,M.Y Sabri,TANKO NWUNUJI POLYCARP,ABU NOR NADIRAH,Onilude Opeyemi Pathological Changes Associated with Aeromonas hydrophila Dose-Dependent Infection and Heat Stress in Red Hybrid Tilapia. (2021): 179 - 187. 10.5152/actavet.2021.20057
MLA ALI Nadiah,M.Y Sabri,TANKO NWUNUJI POLYCARP,ABU NOR NADIRAH,Onilude Opeyemi Pathological Changes Associated with Aeromonas hydrophila Dose-Dependent Infection and Heat Stress in Red Hybrid Tilapia. , 2021, ss.179 - 187. 10.5152/actavet.2021.20057
AMA ALI N,M.Y S,TANKO NWUNUJI P,ABU NOR N,Onilude O Pathological Changes Associated with Aeromonas hydrophila Dose-Dependent Infection and Heat Stress in Red Hybrid Tilapia. . 2021; 179 - 187. 10.5152/actavet.2021.20057
Vancouver ALI N,M.Y S,TANKO NWUNUJI P,ABU NOR N,Onilude O Pathological Changes Associated with Aeromonas hydrophila Dose-Dependent Infection and Heat Stress in Red Hybrid Tilapia. . 2021; 179 - 187. 10.5152/actavet.2021.20057
IEEE ALI N,M.Y S,TANKO NWUNUJI P,ABU NOR N,Onilude O "Pathological Changes Associated with Aeromonas hydrophila Dose-Dependent Infection and Heat Stress in Red Hybrid Tilapia." , ss.179 - 187, 2021. 10.5152/actavet.2021.20057
ISNAD ALI, Nadiah vd. "Pathological Changes Associated with Aeromonas hydrophila Dose-Dependent Infection and Heat Stress in Red Hybrid Tilapia". (2021), 179-187. https://doi.org/10.5152/actavet.2021.20057
APA ALI N, M.Y S, TANKO NWUNUJI P, ABU NOR N, Onilude O (2021). Pathological Changes Associated with Aeromonas hydrophila Dose-Dependent Infection and Heat Stress in Red Hybrid Tilapia. Acta Veterinaria Eurasia, 47(3), 179 - 187. 10.5152/actavet.2021.20057
Chicago ALI Nadiah,M.Y Sabri,TANKO NWUNUJI POLYCARP,ABU NOR NADIRAH,Onilude Opeyemi Pathological Changes Associated with Aeromonas hydrophila Dose-Dependent Infection and Heat Stress in Red Hybrid Tilapia. Acta Veterinaria Eurasia 47, no.3 (2021): 179 - 187. 10.5152/actavet.2021.20057
MLA ALI Nadiah,M.Y Sabri,TANKO NWUNUJI POLYCARP,ABU NOR NADIRAH,Onilude Opeyemi Pathological Changes Associated with Aeromonas hydrophila Dose-Dependent Infection and Heat Stress in Red Hybrid Tilapia. Acta Veterinaria Eurasia, vol.47, no.3, 2021, ss.179 - 187. 10.5152/actavet.2021.20057
AMA ALI N,M.Y S,TANKO NWUNUJI P,ABU NOR N,Onilude O Pathological Changes Associated with Aeromonas hydrophila Dose-Dependent Infection and Heat Stress in Red Hybrid Tilapia. Acta Veterinaria Eurasia. 2021; 47(3): 179 - 187. 10.5152/actavet.2021.20057
Vancouver ALI N,M.Y S,TANKO NWUNUJI P,ABU NOR N,Onilude O Pathological Changes Associated with Aeromonas hydrophila Dose-Dependent Infection and Heat Stress in Red Hybrid Tilapia. Acta Veterinaria Eurasia. 2021; 47(3): 179 - 187. 10.5152/actavet.2021.20057
IEEE ALI N,M.Y S,TANKO NWUNUJI P,ABU NOR N,Onilude O "Pathological Changes Associated with Aeromonas hydrophila Dose-Dependent Infection and Heat Stress in Red Hybrid Tilapia." Acta Veterinaria Eurasia, 47, ss.179 - 187, 2021. 10.5152/actavet.2021.20057
ISNAD ALI, Nadiah vd. "Pathological Changes Associated with Aeromonas hydrophila Dose-Dependent Infection and Heat Stress in Red Hybrid Tilapia". Acta Veterinaria Eurasia 47/3 (2021), 179-187. https://doi.org/10.5152/actavet.2021.20057