Objective: The current study aimed at evaluating the effect of family characteristics of the adolescents upon their healthy lifestyle behaviors. Material and Methods: In this cross-sectional study, 378 high school students were evaluated. As data collection tools; descriptive characteristics form for adolescents and Healthy Lifestyle Behaviors Scale II were employed. Data related to characteristics of adolescents were evaluated by number, percentage and average. To examine the relationship between characteristics of adolescents and scale; variance analysis (ANOVA), t-test and TUKEY test were used as statistical methods. Results: According to the study findings; total mean score of the adolescents for Healthy Lifestyle Behaviors Scale II was 126.31±18.26. In the study; the highest score was obtained from spiritual development while the lowest score was obtained from physical activity. It was identified that there was a statistically significant correlation between family characteristics (family structure, educational status of mother and father, employment status of mother, monthly income level, family relations) (p<0.05). Conclusion: We are of the opinion that study results are important as basic data in demonstrating the effect of family characteristics upon adolescents’ health behaviors while nurses are planning programs that will provide healthy lifestyle behaviors and promote health of adolescents. According to these results, we recommend that nurses, while planning health education programs that protect and improve the health of adolescents, first evaluate the family characteristics of this group and form the content of the training program in line with the evaluation results.