Isatis Glauca Subsp. Sivasica Extract Contributes to Diabetic Wound Healing Process via Increased Collagen and Nitric Oxide Content

Kaan Kaltalıoğlu, (Giresun Üniversitesi, Espiye Meslek Yüksekokulu, Giresun, Türkiye)
Şule Coşkun Cevher (Gazi Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Ankara, Türkiye)
Hittite Journal of Science and Engineering
7 2
Yıl: 2021 Cilt: 8 Sayı: 2 Sayfa Aralığı: 141 - 147 Metin Dili: İngilizce DOI: 10.17350/HJSE19030000224 İndeks Tarihi: 29-07-2022

Isatis Glauca Subsp. Sivasica Extract Contributes to Diabetic Wound Healing Process via Increased Collagen and Nitric Oxide Content

Öz:
Isatis L. genus highly endemic and that is traditionally suggested for wound management in Turkey. In this study, we have reported the effect of I. glauca subsp. sivasica extract on diabetic wound healing process. A diabetic model was generated in Wistar rats using streptozotocin injection. Rats were divided into two main groups: control group and Isatis group. Full thickness excisional skin wounds were created by using a biopsy punch. The Isatis group was treated with single daily dose I. glauca subsp. sivasica extract (50mg/kg). Rats were sacrificed day 3 or 7 after wounding. The dominant phenolic compounds identified with RP-HPLC-DAD from Isatis extract were the benzoic acid and vanillic acid. Isatis extract significantly accelerated wound healing process considering the wound closure rates (WCR). Moreover, the levels of collagen and nitric oxide were elevated on days 3 and 7 by Isatis administration in the diabetic wound tissue. These data suggest that, for the first time, I. glauca subsp. sivasica extract may have the potential to promote the impaired wound healing in patients with diabetes.
Anahtar Kelime: diabetes mellitus collagen Isatis glauca subsp. sivasica wound healing nitric oxide

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • 1. Güner A, Aslan S, Ekim T, Vural M, Babaç MT. Türkiye Bitkileri Listesi (Damarlı Bitkiler), first ed. Flora Araştırmaları Derneği ve Nezahat Gökyiğit Botanik Bahçesi Yayını, İstanbul, 2012.
  • 2. Yıldız N, Karakaş Ö. Qualitative and Quantitative Determination of Tryptanthrin, Indirubin, Indican and Isatin Indole Alkaloids During Vegetative and Flowering Stages in the Roots and Leaves of Isatis constricta P. H. Davis. Tarla Bitkileri Merkez Araştırma Enstitüsü Dergisi 28 (2019) 59–66.
  • 3. Karakoca K, Özüsağlam MA, Çakmak YS, Erkul SK. Antioxidative, antimicrobial and cytotoxic properties of isatis floribunda boiss. ex bornm. Extracts. EXCLI Journal 12 (2013) 150–167.
  • 4. Küpeli E, Orhan İ, Yeşilada E. Evaluation of Some Plants Used in Turkish Folk Medicine for Their Anti-inflammatory and Antinociceptive Activities. Pharmaceutical Biology 45 (2007) 547–555.
  • 5. Hamburger M. Isatis tinctoria - From the Rediscovery of an Ancient Medicinal Plant Towards a Novel Anti-Inflamatory Phytopharmaceutical. Phytochemistry Reviews 1 (2002) 333–344.
  • 6. Tuzlacı E, Doğan A. Turkish folk medicinal plants, IX: Ovacık (Tunceli). Marmara Pharmaceutical Journal 14 (2010) 136–143.
  • 7. Rezaeipoor R, Ziaei Sanei L, Kamalinejad M. Effects of Isatis cappadocica on humoral immune responses. Fitoterapia 71 (2000) 14-18.
  • 8. Ozturk M, Uysal I, Gucel S, Altundag E, Dogan Y, Baslar S. Medicinal Uses of Natural Dye-Yielding Plants in Turkey. Research Journal of Textile and Apparel 17 (2013) 69–80.
  • 9. Baytop T. Therapy with Plants in Turkey (Past & Present), second ed. Nobel Tıp, İstanbul, 1999.
  • 10. Guo S, DiPietro LA. Factors Affecting Wound Healing. Journal of Dental Research 89 (2010) 219–229.
  • 11. Li H, Fu X, Zhang L, Huang Q, Wu Z, Sun T. Research of PDGF-BB gel on the wound healing of diabetic rats and its pharmacodynamics. Journal of Surgical Research 145 (2008) 41–48.
  • 12. Kaltalioglu K, Balabanli B, Coskun-Cevher S. Phenolic, Antioxidant, Antimicrobial, and In-vivo Wound Healing Properties of Potentilla erecta L. Root Extract in Diabetic Rats. Iranian Journal of Pharmaceutical Research 19 (2020) 264–274.
  • 13. Brett D. A review of collagen and collagen-based wound dressings. Wounds 20 (2008) 347–356.
  • 14. Schäfer M, Werner S. Oxidative stress in normal and impaired wound repair. Pharmacological Research 58 (2008) 165–171.
  • 15. Blakytny R, Jude E. The molecular biology of chronic wounds and delayed healing in diabetes. Diabetic Medicine 23 (2006) 594–608.
  • 16. Malone-Povolny MJ, Maloney SE, Schoenfisch MH. Nitric Oxide Therapy for Diabetic Wound Healing. Advanced Healthcare Materials 8 (2019) e1801210.
  • 17. Bektas E, Kaltalioglu K, Sahin H, Turkmen Z, Kandemir A. Analysis of phenolic compounds, antioxidant and antimicrobial properties of some endemic medicinal plants. International Journal of Secondary Metabolite 5 (2018) 75–86.
  • 18. Tsuda K, Nakatani T, Sugama J, Okuwa M, Sanada H. Influence of the timing of switching a protein-free to a protein-containing diet on the wound healing process in a rat all-layer skin defect. International Wound Journal 7 (2010) 135–146.
  • 19. Miranda KM, Espey MG, Wink DA. A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide 5 (2001) 62–71.
  • 20. Miceli N, Filocamo A, Ragusa S, Cacciola F, Dugo P, Mondello L, Celano M, Maggisano V, Taviano MF. Chemical characterization and biological activities of phenolic-rich fraction from cauline leaves of Isatis tinctoria L. (Brassicaceae) growing in Sicily, Italy. Chemistry & Biodiversity 14 (2017) e1700073.
  • 21. dos Santos Gramma LS, Marques FM, Vittorazzi C, de Andrade, TAM, Frade MAC, de Andrade TU, Endringer DC, Scherer R, Fronza M. Struthanthus vulgaris ointment prevents an over expression of inflammatory response and accelerates the cutaneous wound healing. Journal of Ethnopharmacology 190 (2016) 319–327.
  • 22. Kumar S, Prahalathan P, Raja B. Antihypertensive and antioxidant potential of vanillic acid, a phenolic compound in l-NAMEinduced hypertensive rats: A dose-dependence study. Redox Report 16 (2011) 208–215.
  • 23. Ugusman A, Zakaria Z, Chua KH, Nordin NAMM, Abdullah Mahdy Z. Role of rutin on nitric oxide synthesis in human umbilical vein endothelial cells. Scientific World Journal (2014) 2014: 1–9.
  • 24. Choi SJ, Lee SN, Kim K, Joo da H, Shin S, Lee J, Lee HK, Kim J, Kwon SB, Kim MJ, Ahn KJ, An IS, An S, Cha HJ. Biological effects of rutin on skin aging. International Journal of Molecular Medicine 38 (2016) 357–363.
  • 25. Kızıl S, Turk M, Çakmak Ö, Özgüven M, Khawar KM. Microelement Contents and Fatty Acid Compositions of some Isatis Species Seeds. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 37 (2009) 175–178.
  • 26. Lambertucci RH, Leandro CG, Vinolo MA, Nachbar RT, Dos Reis Silveira L, Hirabara SM, Curi R, Pithon-Curi TC. The effects of palmitic acid on nitric oxide production by rat skeletal muscle: mechanism via superoxide and iNOS activation. Cellular Physiology and Biochemistry 30 (2012) 1169–1180.
  • 27. de Lima TM, de Sa Lima L, Scavone C, Curi R. Fatty acid control of nitric oxide production by macrophages. FEBS Letters 580 (2006) 3287–3295.
  • 28. Witte MB, Thornton FJ, Efron DT, Barbul A. Enhancement of fibroblast collagen synthesis by nitric oxide. Nitric Oxide 4 (2000) 572–582.
  • 29. Witte MB, Kiyama T, Barbul A. Nitric oxide enhances experimental wound healing in diabetes. British Journal of Surgery 89 (2002) 1594–1601.
  • 30. Achuth HN, Moochhala SM, Mahendran R, Tan WT. Nitrosoglutathione triggers collagen deposition in cutaneous wound repair. Wound Repair and Regeneration 13 (2005) 383–389.
  • 31. Luo J, Chen AF. Nitric oxide: a newly discovered function on wound healing. Acta Pharmacologica Sinica 26 (2005) 259–264.
APA Kaltalioglu K, COŞKUN CEVHER Ş (2021). Isatis Glauca Subsp. Sivasica Extract Contributes to Diabetic Wound Healing Process via Increased Collagen and Nitric Oxide Content. , 141 - 147. 10.17350/HJSE19030000224
Chicago Kaltalioglu Kaan,COŞKUN CEVHER ŞULE Isatis Glauca Subsp. Sivasica Extract Contributes to Diabetic Wound Healing Process via Increased Collagen and Nitric Oxide Content. (2021): 141 - 147. 10.17350/HJSE19030000224
MLA Kaltalioglu Kaan,COŞKUN CEVHER ŞULE Isatis Glauca Subsp. Sivasica Extract Contributes to Diabetic Wound Healing Process via Increased Collagen and Nitric Oxide Content. , 2021, ss.141 - 147. 10.17350/HJSE19030000224
AMA Kaltalioglu K,COŞKUN CEVHER Ş Isatis Glauca Subsp. Sivasica Extract Contributes to Diabetic Wound Healing Process via Increased Collagen and Nitric Oxide Content. . 2021; 141 - 147. 10.17350/HJSE19030000224
Vancouver Kaltalioglu K,COŞKUN CEVHER Ş Isatis Glauca Subsp. Sivasica Extract Contributes to Diabetic Wound Healing Process via Increased Collagen and Nitric Oxide Content. . 2021; 141 - 147. 10.17350/HJSE19030000224
IEEE Kaltalioglu K,COŞKUN CEVHER Ş "Isatis Glauca Subsp. Sivasica Extract Contributes to Diabetic Wound Healing Process via Increased Collagen and Nitric Oxide Content." , ss.141 - 147, 2021. 10.17350/HJSE19030000224
ISNAD Kaltalioglu, Kaan - COŞKUN CEVHER, ŞULE. "Isatis Glauca Subsp. Sivasica Extract Contributes to Diabetic Wound Healing Process via Increased Collagen and Nitric Oxide Content". (2021), 141-147. https://doi.org/10.17350/HJSE19030000224
APA Kaltalioglu K, COŞKUN CEVHER Ş (2021). Isatis Glauca Subsp. Sivasica Extract Contributes to Diabetic Wound Healing Process via Increased Collagen and Nitric Oxide Content. Hittite Journal of Science and Engineering, 8(2), 141 - 147. 10.17350/HJSE19030000224
Chicago Kaltalioglu Kaan,COŞKUN CEVHER ŞULE Isatis Glauca Subsp. Sivasica Extract Contributes to Diabetic Wound Healing Process via Increased Collagen and Nitric Oxide Content. Hittite Journal of Science and Engineering 8, no.2 (2021): 141 - 147. 10.17350/HJSE19030000224
MLA Kaltalioglu Kaan,COŞKUN CEVHER ŞULE Isatis Glauca Subsp. Sivasica Extract Contributes to Diabetic Wound Healing Process via Increased Collagen and Nitric Oxide Content. Hittite Journal of Science and Engineering, vol.8, no.2, 2021, ss.141 - 147. 10.17350/HJSE19030000224
AMA Kaltalioglu K,COŞKUN CEVHER Ş Isatis Glauca Subsp. Sivasica Extract Contributes to Diabetic Wound Healing Process via Increased Collagen and Nitric Oxide Content. Hittite Journal of Science and Engineering. 2021; 8(2): 141 - 147. 10.17350/HJSE19030000224
Vancouver Kaltalioglu K,COŞKUN CEVHER Ş Isatis Glauca Subsp. Sivasica Extract Contributes to Diabetic Wound Healing Process via Increased Collagen and Nitric Oxide Content. Hittite Journal of Science and Engineering. 2021; 8(2): 141 - 147. 10.17350/HJSE19030000224
IEEE Kaltalioglu K,COŞKUN CEVHER Ş "Isatis Glauca Subsp. Sivasica Extract Contributes to Diabetic Wound Healing Process via Increased Collagen and Nitric Oxide Content." Hittite Journal of Science and Engineering, 8, ss.141 - 147, 2021. 10.17350/HJSE19030000224
ISNAD Kaltalioglu, Kaan - COŞKUN CEVHER, ŞULE. "Isatis Glauca Subsp. Sivasica Extract Contributes to Diabetic Wound Healing Process via Increased Collagen and Nitric Oxide Content". Hittite Journal of Science and Engineering 8/2 (2021), 141-147. https://doi.org/10.17350/HJSE19030000224
Sayfa Oluşturulma Tarihi
23-04-2024 21:46

İLETİŞİM

TÜBİTAK ULAKBİM TR Dizin

Yüzüncüyıl, İşçi Blokları Mahallesi

Muhsin Yazıcıoğlu Caddesi No:51/C

06530 Çankaya / ANKARA +90 (312) 298 92 00

trdizin@tubitak.gov.tr

TÜBİTAK ULAKBİM Ulusal Akademik Ağ ve Bilgi Merkezi Cahit Arf Bilgi Merkezi © Tüm Hakları Saklıdır. Gizlilik Politikası Kullanım Şartları