In Vitro Bioassay of Purpureocillium lilacinum and Bacillusthuringiensis for Control of Meloidogyne incognita on Black Pepper(Piper nigrum L.) in Sarawak, Malaysia, Northern Borneo

Guan, Chen; LEONG, SUI SIEN; Leong, Stephen; Beattie, Andrew

doi:10.51963/jers.v23i1.1960

In Vitro Bioassay of Purpureocillium lilacinum and Bacillusthuringiensis for Control of Meloidogyne incognita on Black Pepper(Piper nigrum L.) in Sarawak, Malaysia, Northern Borneo

Sui Sien LEONG, (Faculty of Agricultural Science and Forestry, Universiti Putra Malaysia Campus Bintulu Sarawak, Nyabau Road, MALEZYA)

Stephen Chan Teck LEONG, (Faculty of Agricultural Science and Forestry, Universiti Putra Malaysia Campus Bintulu Sarawak, Nyabau Road, MALEZYA)

Chen Guan PAU, (Faculty of Agricultural Science and Forestry, Universiti Putra Malaysia Campus Bintulu Sarawak, Nyabau Road, MALEZYA)

G. Andrew C. BEATTIE (Centre for Plant and Food Science, University of Western Sydney, Locked Bag, AVUSTRALYA)

Journal of the Entomological Research Society

1 0

Yıl: 2021 Cilt: 23 Sayı: 1 Sayfa Aralığı: 41 - 59 Metin Dili: İngilizce DOI: 10.51963/jers.v23i1.1960 İndeks Tarihi: 21-05-2022

In Vitro Bioassay of Purpureocillium lilacinum and Bacillusthuringiensis for Control of Meloidogyne incognita on Black Pepper(Piper nigrum L.) in Sarawak, Malaysia, Northern Borneo

Öz:

This study aimed to evaluate strains of the entomopathogenic fungus Purpureocillium lilacinum and the bacterium Bacillus thuringiensis from Bintulu, in north-eastern Sarawak, Malaysia for their invitro nematicidal properties against different developmental stages of Meloidogyne incognita. Tenindigenous novel strains of P. lilacinum, a commercial strain of the fungus (P. lilacinum M), and a strainof B. thuringiensis carrying Cry6 and Cry14 gene sequences were screened for parasitism against M.incognita females and eggs, egg hatching inhabitation and 2nd stage of active juveniles (J2) mortality. Ourstudy demonstrates that P. lilacinum A and P. lilacinum B were the most effective biocontrol agent againstM.incognita females, eggs and J2 due to highest infection of females, eggs and significant decrease inegg hatching events. P. lilacinum A, P. lilacinum B, and P. lilacinum M (positive control) demonstratedhighly significant infection (>90%, P≤0.01) on M.incognita female nematodes. The present study revealedthat spore suspension (105 spore/mL) of P. lilacinum A, P. lilacinum B and P. lilacinum M resulted in 78.8%,66.0% and 73.4% parasitism on eggs, respectively. P. lilacinum A, P. lilacinum B and P. lilacinum M withlow mortality of 6.0%, 5.5% and 5.7%, respectively, showed significant (P<0.05) differences in mortalityeffect on J2 of M.incognita as compared with control treatment (2.3%). Hatching of M. incognita eggsincubated in spore suspension of P. lilacinum A, P. lilacinum B and P. lilacinum M for seven days werereduced by 89% when corrected for control mortality (26%).

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APA	LEONG S, Leong S, Guan C, Beattie A (2021). In Vitro Bioassay of Purpureocillium lilacinum and Bacillusthuringiensis for Control of Meloidogyne incognita on Black Pepper(Piper nigrum L.) in Sarawak, Malaysia, Northern Borneo. , 41 - 59. 10.51963/jers.v23i1.1960
Chicago	LEONG SUI SIEN,Leong Stephen,Guan Chen,Beattie Andrew In Vitro Bioassay of Purpureocillium lilacinum and Bacillusthuringiensis for Control of Meloidogyne incognita on Black Pepper(Piper nigrum L.) in Sarawak, Malaysia, Northern Borneo. (2021): 41 - 59. 10.51963/jers.v23i1.1960
MLA	LEONG SUI SIEN,Leong Stephen,Guan Chen,Beattie Andrew In Vitro Bioassay of Purpureocillium lilacinum and Bacillusthuringiensis for Control of Meloidogyne incognita on Black Pepper(Piper nigrum L.) in Sarawak, Malaysia, Northern Borneo. , 2021, ss.41 - 59. 10.51963/jers.v23i1.1960
AMA	LEONG S,Leong S,Guan C,Beattie A In Vitro Bioassay of Purpureocillium lilacinum and Bacillusthuringiensis for Control of Meloidogyne incognita on Black Pepper(Piper nigrum L.) in Sarawak, Malaysia, Northern Borneo. . 2021; 41 - 59. 10.51963/jers.v23i1.1960
Vancouver	LEONG S,Leong S,Guan C,Beattie A In Vitro Bioassay of Purpureocillium lilacinum and Bacillusthuringiensis for Control of Meloidogyne incognita on Black Pepper(Piper nigrum L.) in Sarawak, Malaysia, Northern Borneo. . 2021; 41 - 59. 10.51963/jers.v23i1.1960
IEEE	LEONG S,Leong S,Guan C,Beattie A "In Vitro Bioassay of Purpureocillium lilacinum and Bacillusthuringiensis for Control of Meloidogyne incognita on Black Pepper(Piper nigrum L.) in Sarawak, Malaysia, Northern Borneo." , ss.41 - 59, 2021. 10.51963/jers.v23i1.1960
ISNAD	LEONG, SUI SIEN vd. "In Vitro Bioassay of Purpureocillium lilacinum and Bacillusthuringiensis for Control of Meloidogyne incognita on Black Pepper(Piper nigrum L.) in Sarawak, Malaysia, Northern Borneo". (2021), 41-59. https://doi.org/10.51963/jers.v23i1.1960

APA	LEONG S, Leong S, Guan C, Beattie A (2021). In Vitro Bioassay of Purpureocillium lilacinum and Bacillusthuringiensis for Control of Meloidogyne incognita on Black Pepper(Piper nigrum L.) in Sarawak, Malaysia, Northern Borneo. Journal of the Entomological Research Society, 23(1), 41 - 59. 10.51963/jers.v23i1.1960
Chicago	LEONG SUI SIEN,Leong Stephen,Guan Chen,Beattie Andrew In Vitro Bioassay of Purpureocillium lilacinum and Bacillusthuringiensis for Control of Meloidogyne incognita on Black Pepper(Piper nigrum L.) in Sarawak, Malaysia, Northern Borneo. Journal of the Entomological Research Society 23, no.1 (2021): 41 - 59. 10.51963/jers.v23i1.1960
MLA	LEONG SUI SIEN,Leong Stephen,Guan Chen,Beattie Andrew In Vitro Bioassay of Purpureocillium lilacinum and Bacillusthuringiensis for Control of Meloidogyne incognita on Black Pepper(Piper nigrum L.) in Sarawak, Malaysia, Northern Borneo. Journal of the Entomological Research Society, vol.23, no.1, 2021, ss.41 - 59. 10.51963/jers.v23i1.1960
AMA	LEONG S,Leong S,Guan C,Beattie A In Vitro Bioassay of Purpureocillium lilacinum and Bacillusthuringiensis for Control of Meloidogyne incognita on Black Pepper(Piper nigrum L.) in Sarawak, Malaysia, Northern Borneo. Journal of the Entomological Research Society. 2021; 23(1): 41 - 59. 10.51963/jers.v23i1.1960
Vancouver	LEONG S,Leong S,Guan C,Beattie A In Vitro Bioassay of Purpureocillium lilacinum and Bacillusthuringiensis for Control of Meloidogyne incognita on Black Pepper(Piper nigrum L.) in Sarawak, Malaysia, Northern Borneo. Journal of the Entomological Research Society. 2021; 23(1): 41 - 59. 10.51963/jers.v23i1.1960
IEEE	LEONG S,Leong S,Guan C,Beattie A "In Vitro Bioassay of Purpureocillium lilacinum and Bacillusthuringiensis for Control of Meloidogyne incognita on Black Pepper(Piper nigrum L.) in Sarawak, Malaysia, Northern Borneo." Journal of the Entomological Research Society, 23, ss.41 - 59, 2021. 10.51963/jers.v23i1.1960
ISNAD	LEONG, SUI SIEN vd. "In Vitro Bioassay of Purpureocillium lilacinum and Bacillusthuringiensis for Control of Meloidogyne incognita on Black Pepper(Piper nigrum L.) in Sarawak, Malaysia, Northern Borneo". Journal of the Entomological Research Society 23/1 (2021), 41-59. https://doi.org/10.51963/jers.v23i1.1960