PURIFICATION OF OVALBUMIN FROM EGG WHITE USING MOLECULAR IMPRINTED CRYOGELS

Çimen, Duygu; Denizli, Adil

doi:10.15671/hjbc.918700

PURIFICATION OF OVALBUMIN FROM EGG WHITE USING MOLECULAR IMPRINTED CRYOGELS

Duygu ÇİMEN, (Hacettepe Üniversitesi, Kimya Bölümü, Ankara, Türkiye)

Adil DENİZLİ (Hacettepe Üniversitesi, Kimya Bölümü, Ankara, Türkiye)

Hacettepe Journal of Biology and Chemistry

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Yıl: 2022 Cilt: 50 Sayı: 1 Sayfa Aralığı: 65 - 76 Metin Dili: İngilizce DOI: 10.15671/hjbc.918700 İndeks Tarihi: 29-07-2022

PURIFICATION OF OVALBUMIN FROM EGG WHITE USING MOLECULAR IMPRINTED CRYOGELS

Öz:

I n recent years, food allergy is one of the most important problems worldwide. Ovalbumin is one of the main allergens found in egg whites. In this study, ovalbumin imprinted and non-imprinted poly(hydroxyethyl methacrylate-methacrylic acid (poly(HEMA-MAA)) cryogels were synthesized using molecular imprinting method. After characterization of ovalbumin imprinted cryogels, the effect of flow rate, pH buffer, einitial ovalbumin concentration, ionic strength, and temperature parameters are examined. The maximum ovalbumin adsorption amount was determined to be 37.78 mg/g of ovalbumin imprinted cryogels at pH 5.5, 25°C and a flow rate of 0.5 mL/min. The selectivity studies of ovalbumin imprinted and non-imprinted cryogels were performed using lysozyme and transferrin as competitor molecules. In addition, reusability experiments of ovalbumin imprinted cryogels were performed. The purity of ovalbumin was determined from egg white using sodium-dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The purity of ovalbumin was found to be approximately 85.4%.

Anahtar Kelime: purification molecular imprinting cryogel ovalbumin

Moleküler Baskılanmış Kriyojeller Kullanılarak Yumurta Akından Ovalbumin Saflaştırılması

Öz:

Son yıllarda, dünya çapında gıda alerjisi en önemli sorunlardan biridir. Ovalbumin yumurta akında bulunan ana alerjanlardan biridir. Bu çalışmada, ovalbumin baskılanmış ve baskılanmamış poli(hidroksietil metakrilat-metakrilik asit) poli(HEMA-MAA) kriyojeller moleküler baskılama yöntemi kullanılarak sentezlenmiştir. Ovalbumin baskılanmış kriyojellerin karakterizasyonundan sonra, akış hızı, pH tamponu, ilk ovalbümin konsantrasyonu, iyonik kuvvet ve sıcaklık parametrelerinin etkisi incelenir. Maksimum ovalbumin adsorpsiyon miktarı, ovalbumin baskılanmış kriyojel için pH 5.5, 25°C ve 0.5 mL/dk akış hızında 37.78 mg/g olarak belirlenmiştir. Ovalbumin baskılanmış ve baskılanmamış kriyojellerin seçicilik çalışmaları, yarışmacı moleküller olarak lizozim ve transferrin kullanılarak gerçekleştirilmiştir. Ayrıca ovalbumin baskılanmış kriyojellerin yeniden kullanılabilirlik deneyleri gerçekleştirilmiştir. Ovalbuminin saflığı, sodyum-dodesil sülfat poliakrilamid jel elektroforezi (SDSPAGE) kullanılarak yumurta akından belirlenmiştir. Ovalbuminin saflığı yaklaşık% 85.4 bulunmuştur.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. J.A. Huntington, P.E. Stein, Structure and properties of ovalbumin, J. Chromatogr. B Biomed. Sci. Appl., 756 (2001) 189-198.
2. M.D.A. Dantas, H.A. Tenório, Thiago I.B. Lopes, H.J.V. Pereira, A.J. Marsaioli, I.M. Figueiredo, J.C.C. Santos, Interactions of tetracyclines with ovalbumin, the main allergen protein from egg white: Spectroscopic and electrophoretic studies, Int. J. Biol. Macromol., 102 (2017) 505-514.
3. X. Fu, Q. Liu, C. Tang, J. Luo, X. Wu, L. Lu, Z. Cai, Study on structural, rheological and foaming properties of ovalbumin by ultrasound-assisted glycation with xylose, Ultrason, Sonochem., 58 (2019) 104644.
4. J. Miloševic ́, J. Petric ́, B. Jovčic ́, B. Jankovic ́, N. Polovic ́, Exploring the potential of infrared spectroscopy in qualitative and quantitative monitoring of ovalbumin amyloid fibrillation, Spectrochim. Acta A Mol Biomol. Spectrosc., 229 (2020) 117882.
5. J.A. Huntington, P.E. Stein, Structure and properties of ovalbumin, J. Chromatogr. B, 756 (2001) 189-198.
6. E.D. Abeyrathne, H.Y. Lee, D.U. Ahn, Egg white proteins and their potential use in food processing or as nutraceutical and pharmaceutical agents-a review, Poult. Sci. J., 92 (2013), 3292-3299.
7. H.Y. Park, T.J. Yoon, H.H. Kim, Y.S. Han, H.D. Choi, Changes in the antigenicity and allergenicity of ovalbumin in chicken egg white by N-acetylglucosaminidase, Food Chem., 217 (2017) 342-345.
8. L.Z. Sun, S. Elsayed, T.B. Aasen, T. Van Do, N.P. Aardal, E. Florvaag, K. Vaali, Comparison between ovalbumin and ovalbumin peptide 323–339 responses in allergic mice: Humoral and cellular aspects, Scand. J. Immunol., 71 (2010) 329-335.
9. W.H. Yang, Z.C. Tu, H. Wang, X. Li, M. Tian, High-intensity ultrasound enhances the immunoglobulin IgG and IgE binding of ovalbumin, J. Sci. Food Agric., 97 (2017) 2714- 2720.
10. M.M. Pereira, R.A.P. Cruz, M.R. Almeida, Á.S. Lima, J.A.P. Coutinho, M.G. Freire, Single-step purification of ovalbumin from egg white using aqueous biphasic systems, Process Biochem., 51 (2016) 781-791.
11. N. Habibi, S. Christau, L.J. Ochyl, Z. Fan, A.H. Najafabadi, M. Kuehnhammer, M. Zhang, M. Helgeson, R. Klitzing, J.J. Moon, J. Lahann, Engineered ovalbumin nanoparticles for cancer immunotherapy, Adv. Therap., 3 (2020) 2000100.
12. W. Zou, F.K. Mourad, X. Zhang, D.U. Ahn, Z. Cai, Y. Jin, Phase separation behavior and characterization of ovalbumin and propylene glycol alginate complex coacervates, Food Hydrocoll., 108 (2020) 105978.
13. V. Verhasselt, J. Genuneit, J.R. Metcalfe, M.K. Tulic, A. Rekima, D.J. Palmer, S.L. Prescott, Ovalbumin in breastmilk is associated with a decreased risk of IgE-mediated egg allergy in children, Allergy., 75 (2020) 1463-1466.
14. I. Roy, M.V. S. Rao, M.N. Gupta, An integrated process for purification of lysozyme, ovalbumin, and ovomucoid from hen egg white, Appl. Biochemi. Biotechnol., 111 (2003) 55- 63.
15. B. Jiang, J. Na, L. Wang, D. Li, C. Liu, Z. Feng, Eco-innovation in reusing food by-products: Separation of ovalbumin from salted egg white using aqueous two-phase system of PEG 1000/(NH4)2SO4, Polymers, 11 (2019) 238.
16. K. Liu, S. Chen, H. Chen, P. Tong, J. Gao, Cross-linked ovalbumin catalyzed by polyphenol oxidase: Preparation, structure and potential allergenicity, Int. J. Biol. Macromol., 107 (2018) 2057-2064.
17. S. Jalili-Firoozinezhad, M. Filippi, F. Mohabatpour, D. Letourneur, A. Scherberich, Chicken egg white: Hatching of a new old biomaterial, Mater. Today Commun., 40 (2020) 193-214.
18. H. Xia, L. Wang, C. Li, B. Tian, Q. Li, H. Zhao, Q. Bai, Synthesis of fully porous silica microspheres with high specific surface area for fast HPLC separation of intact proteins and digests of ovalbumin, Microchim. Acta, 187 (2020) 382.
19. H. Wang, Q. Sun, J. Tan, Y. Hu, W. Yan, Z. Li, Z. Tu, Conformational alteration and the glycated sites in ovalbumin during vacuum freeze-drying induced glycation: A study using conventional spectrometry and liquid chromatography high resolution mass spectrometry, Food Chem., 318 (2020) 126519.
20. F. Füss, A. Criscuolo, K. Cook, K. Scheffler, J. Bones, Cracking proteoform complexity of ovalbumin with anion-exchange chromatography high-resolution mass spectrometry under native conditions, J. Proteome Res., 18 (2019) 3689-3702.
21. B. Jiang, J. Na, L. Wang, D. Li, C. Liu, Z. Feng, Reutilization of food waste: One-step extration, purification and characterization of ovalbumin from salted egg white by aqueous two-phase flotation, Foods, 8 (2019) 286.
22. D. Çimen, A. Denizli, Immobilize metal affinity monolithic cryogels for cytochrome C purification, Colloids Surf. B: Biointerfaces, 93 (2012) 29-35.
23. V.M. Gun’ko, I.N. Savina, S.V. Mikhalovsky, Cryogels: Morphological, structural and adsorption characterization, Adv. Coll. Int. Sci., 187 (2013) 1-46.
24. Y. Saylan, A. Denizli A. Supermacroporous composite cryogels in biomedical applications, Gels, 5 (2019) 20.
25. A. Memic, T. Colombani, L.J. Eggermont, M. Rezaeeyazdi, J. Steingold, Z.J. Rogers, K.J. Navare, H.S. Mohammed, S.A. Bencherif, Latest advances in cryogel technology for biomedical applications, Adv. Therap., 2 (2019) 1800114.
26. Z.J. Rogers, S.A. Bencherif, Cryogelation and cryogels, Gels, 5 (2019) 46.
27. V.I. Lozinsky, I.Y. Galaev, F.M. Plieva, I.N. Savina, H. Jungvid, B. Mattiasson, Polymeric cryogels as promising materials of biotechnological interest, Trends Biotechnol., 21 (2003) 445-451.
28. V.I. Lozinsky, A brief history of polymeric cryogels, Adv. Polym. Sci., 263 (2014) 1-48.
29. A. Kumar, Supermacroporous cryogels: biomedical and biotechnological applications, Taylor Francis, 480 (2016) 52.
30. D. Çimen, N. Bereli, M. Andaç, A. Denizli, Molecularly imprinted cryogel columns for Concanavalin A purification from jack bean extract, Sep Sci plus., (2018) 1-10.
31. S. Asliyüce, N. Bereli, L. Uzun, M.A. Onur, R. Say, A. Denizli, Ion-imprinted supermacroporous cryogel, for in vitro removal of iron out of human plasma with beta thalassemia, Sep. Purif. Technol., 73 (2010) 243-249.
32. G. Baydemir, N. Bereli, M. Andac ̧, R. Say, I.Y. Galaev, A. Denizli, Supermacroporous poly(hydroxyethyl methacrylate) based cryogel with embedded bilirubin imprinted particles, React. Funct. Polym., 69 (2009) 36-42.
33. M. Andac, I.Y. Galaev, A. Denizli, Affinity based and molecularly imprinted cryogels: Applications in biomacromolecule purification, J. Chromatogr. B, 1021 (2016) 69-80.
34. N. Bereli, H. Yavuz, A. Denizli, A. Protein chromatography by molecular imprinted cryogels, J. Liq Chrom. Relat. Tech., 43 (2020) 657-670.
35. Y. Cui, Z. He, Y. Xu, Y. Su, L. Ding, Y. Li, Fabrication of molecularly imprinted polymers with tunable adsorption capability based on solvent-responsive cross-linker, Chem. Eng. J., 405 (2021) 126608.
36. D. Çimen, I. Göktürk, F. Yılmaz, Removal of iron by chelation with molecularly imprinted supermacroporous cryogel, Artif. Cells Nanomed. Biotechnol., 44 (2016) 1158-1166.
37. X. Zhou, W. Li, X. He, L. Chen, Recent advances in the study of protein imprinting, Sep. Purif. Rev., 36 (2007) 257-283.
38. T. Takeuchi, T. Hishiya, Molecular imprinting of proteins emerging as a tool for protein recognition, Org. Biomol. Chem., 6 (2008) 2459-2467.
39. T.S. Bedwell, N. Anjum, Y. Ma, J. Czulak, A. Poma, E. Piletska, M.J. Whitcombe, S.A. Piletsky, New Protocol for optimization of polymer composition for imprinting of peptides and proteins, RSC Adv., 9 (2009) 27849-27855.
40. A.A. Al-Tubuly, SDS-PAGE and Western Blotting, Methods Mol. Med., 40 (2000) 391-405.
41. Y. Shimazaki, A. Takahashi, Antibacterial activity of lysozymebinding proteins from chicken egg white, J. Microbiol. Methods, 154 (2018) 19-24.
42. K.V. Abrosimova, O.V. Shulenina, S.V. Paston, FTIR study of secondary structure of bovine serum albumin and ovalbumin, J. Phys. Conf. Ser. 769 (2016) 012016.
43. N. Xiao, W.H.Y. Tu, N. Xiao, W. He, Y. Zhao, Y. Yao, M. Xu, H. Du, N. Wu, Y. Tu, Effect of pH and xanthan gum on emulsifying property of ovalbumin stabilized oil-in water emulsions, LWT., 147 (2021) 111621.
44. I. Perçin, G. Şener, A.H. Demirçelik, N. Bereli, A. Denizli, Comparison of two different reactive dye immobilized PHEMA cryogel discs for purification of lysozyme, Appl. Biochem. Biotechnol., 175 (2015) 2795-2805.
45. K. Hamade, I. Göktürk, N. Bereli, D. Türkmen, A. Elkak, A. Denizli, A dye-affinity cryogel membrane for malate dehydrogenase purification from Saccharomyces cerevisiae, J. Biomater. Sci. Polym. Ed., 31 (2020) 38-52.
46. B. Demirci, N. Bereli, S. Aslıyüce, G. Baydemir, A. Denizli, A. (2017). Protein c recognition by ion-coordinated imprinted monolithic cryogels, J. Sep. Sci., 40 (2017) 1610-1620.
47. N. Bereli, M. Andaç, G. Baydemir, R. Say, I.Y. Galaev, A. Denizli, Protein recognition via ion-coordinated molecularly imprinted supermacroporous cryogels, J. Chromatogr. A, 1190 (2008) 18-26.
48. I. Bernabe, J.M. Gomez, E. Dıez, P. Saez, A. Rodrıguez, Optimization and adsorption-based recovery of cobalt using activated disordered mesoporous carbons, Adv. Mater. Sci. Eng., (2019) 1-10.
49. J. H. Lee, H. D. Paik, Anticancer and immunomodulatory activity of egg proteins and peptides: a review, Poult. Sci., 98 (2019) 6505-6516.
50. D. Çimen, D. Türkmen, A. Denizli, Poly-L-histidine immobilized cryogels for lysozyme purification, Adsorp. Sci. Technol., (2016) 1-19.
51. F. Geng, Y. Xie, J. Wang, S. Li, Y. Jin, M. Ma, Large-scale purification of ovalbumin using polyethylene glycol precipitation and isoelectric precipitation, Poult. Sci., 98 (2019), 1545-1550.
52. C. Zheng, Y. Shi-Yu, W. Nan, X. Jia-Wei, C. Qing, Preparation of tungstotellurate(VI)-coated magnetic nanoparticles for separation and purification of ovalbumin in egg white, Chinese J. Anal. Chem., 47 (2019) 1302-1308.
53. M.M. Pereira, R.A.P. Cruz, M.R. Almeida, Á.S. Lima, J.A.P. Coutinho, M.G. Freire, Single-step purification of ovalbumin from egg white using aqueous biphasic systems, Process Biochem., 51 (2016) 781-791.
54. B. Jiang, J. Na, L. Wang, D. Li, C. Liu, Z. Feng, Reutilization of food waste: One-step extration, purification and characterization of ovalbumin from salted egg white by aqueous two-phase flotation, Foods, 8 (2019) 286.
55. T. Croguennec, F. Nau, S. Pezennec, G. Brule, Simple rapid procedure for preparation of large quantities of ovalbumin, J. Agric. Food. Chem., 48 (2000) 4883-4889.

APA	Çimen D, Denizli A (2022). PURIFICATION OF OVALBUMIN FROM EGG WHITE USING MOLECULAR IMPRINTED CRYOGELS. , 65 - 76. 10.15671/hjbc.918700
Chicago	Çimen Duygu,Denizli Adil PURIFICATION OF OVALBUMIN FROM EGG WHITE USING MOLECULAR IMPRINTED CRYOGELS. (2022): 65 - 76. 10.15671/hjbc.918700
MLA	Çimen Duygu,Denizli Adil PURIFICATION OF OVALBUMIN FROM EGG WHITE USING MOLECULAR IMPRINTED CRYOGELS. , 2022, ss.65 - 76. 10.15671/hjbc.918700
AMA	Çimen D,Denizli A PURIFICATION OF OVALBUMIN FROM EGG WHITE USING MOLECULAR IMPRINTED CRYOGELS. . 2022; 65 - 76. 10.15671/hjbc.918700
Vancouver	Çimen D,Denizli A PURIFICATION OF OVALBUMIN FROM EGG WHITE USING MOLECULAR IMPRINTED CRYOGELS. . 2022; 65 - 76. 10.15671/hjbc.918700
IEEE	Çimen D,Denizli A "PURIFICATION OF OVALBUMIN FROM EGG WHITE USING MOLECULAR IMPRINTED CRYOGELS." , ss.65 - 76, 2022. 10.15671/hjbc.918700
ISNAD	Çimen, Duygu - Denizli, Adil. "PURIFICATION OF OVALBUMIN FROM EGG WHITE USING MOLECULAR IMPRINTED CRYOGELS". (2022), 65-76. https://doi.org/10.15671/hjbc.918700

APA	Çimen D, Denizli A (2022). PURIFICATION OF OVALBUMIN FROM EGG WHITE USING MOLECULAR IMPRINTED CRYOGELS. Hacettepe Journal of Biology and Chemistry, 50(1), 65 - 76. 10.15671/hjbc.918700
Chicago	Çimen Duygu,Denizli Adil PURIFICATION OF OVALBUMIN FROM EGG WHITE USING MOLECULAR IMPRINTED CRYOGELS. Hacettepe Journal of Biology and Chemistry 50, no.1 (2022): 65 - 76. 10.15671/hjbc.918700
MLA	Çimen Duygu,Denizli Adil PURIFICATION OF OVALBUMIN FROM EGG WHITE USING MOLECULAR IMPRINTED CRYOGELS. Hacettepe Journal of Biology and Chemistry, vol.50, no.1, 2022, ss.65 - 76. 10.15671/hjbc.918700
AMA	Çimen D,Denizli A PURIFICATION OF OVALBUMIN FROM EGG WHITE USING MOLECULAR IMPRINTED CRYOGELS. Hacettepe Journal of Biology and Chemistry. 2022; 50(1): 65 - 76. 10.15671/hjbc.918700
Vancouver	Çimen D,Denizli A PURIFICATION OF OVALBUMIN FROM EGG WHITE USING MOLECULAR IMPRINTED CRYOGELS. Hacettepe Journal of Biology and Chemistry. 2022; 50(1): 65 - 76. 10.15671/hjbc.918700
IEEE	Çimen D,Denizli A "PURIFICATION OF OVALBUMIN FROM EGG WHITE USING MOLECULAR IMPRINTED CRYOGELS." Hacettepe Journal of Biology and Chemistry, 50, ss.65 - 76, 2022. 10.15671/hjbc.918700
ISNAD	Çimen, Duygu - Denizli, Adil. "PURIFICATION OF OVALBUMIN FROM EGG WHITE USING MOLECULAR IMPRINTED CRYOGELS". Hacettepe Journal of Biology and Chemistry 50/1 (2022), 65-76. https://doi.org/10.15671/hjbc.918700