CHEMICAL CHAPERONE EFFECTS ON ARGININE-VASOPRESSIN RECEPTOR 2 MUTANTS
Yıl: 2022 Cilt: 50 Sayı: 1 Sayfa Aralığı: 37 - 43 Metin Dili: İngilizce DOI: 10.15671/hjbc.669854 İndeks Tarihi: 29-07-2022
CHEMICAL CHAPERONE EFFECTS ON ARGININE-VASOPRESSIN RECEPTOR 2 MUTANTS
Öz: Improper folding of the mutant proteins may finally cause several conformational diseases such as Nephrogenic Diabetes Insipidus (NDI). In recent years, as a therapeutic strategy, chaperone treatment for such diseases is among current issues. In our study, we aimed to analyze the effect of several chemical chaperones on mutant V2 receptors which cause NDI. V2R mutant constructs were introduced into the pLV2R. Mutants were transiently expressed in COS-7 cells. After MTT analyses, cell surface ELISA experiment was performed for understanding the rescue potential of the chaperones of the mutated proteins. As a result, we analyzed that rescue potential of a chemical chaperone depends on both chemical compound and the mutation type. We may conclude that such chaperone treatment studies are valuable for development of the therapeutic strategies.
Anahtar Kelime: Kimyasal Şaperonların Arjinin Vazopressin Reseptör 2 Mutantları Üzerine Etkisi
Öz: Mutant proteinlerin yanlış katlanması, Nefrojenik Diabetes İnsipidus gibi bazı konformasyonel hastalıklara sebep olabilmektedir. Son yıllarda, terapötik bir strateji olarak, şaperon tedavisi bu tür hastalıklar için güncel konular arasındadır. Bu çalışmanın amacı, bazı kimyasal şaperonların Nefrojenik Diabetes İnsipidus’a neden olan V2 reseptör mutantları üzerine etkisinin araştırılmasıdır. V2R mutant örnekleri pLV2R vektöründe oluşturulmuştur. Mutant örnekler geçici trasfeksiyon ile COS-7 hücrelerinde ifade edilmiştir. MTT analizinden sonra, mutant proteinler üzerinde şaperonların kurtarma potansiyellerinin analizi için hücre yüzey ve sandviç ELİZA deneyleri uygulanmıştır. Sonuç olarak, kimyasal şaperonların kurtarma potansiyellerinin hem kullanılan kimyasal bileşiğe hem de mutasyonun tipine göre etki ettiği gözlenmiştir. Bu ve bunun gibi şaperon uygulamalarının terapötik stratejilerin geliştirilmesi açısından değerli olduğu sonucuna varılmıştır.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Erdem Tuncdemir B, Karaduman T, Ozcan Turkmen M, Şahin D, MERGEN H, SAGLAR OZER E (2022). CHEMICAL CHAPERONE EFFECTS ON ARGININE-VASOPRESSIN RECEPTOR 2 MUTANTS. , 37 - 43. 10.15671/hjbc.669854 |
| Chicago | Erdem Tuncdemir Beril,Karaduman Tugce,Ozcan Turkmen Merve,Şahin Dilara,MERGEN HATICE,SAGLAR OZER EMEL CHEMICAL CHAPERONE EFFECTS ON ARGININE-VASOPRESSIN RECEPTOR 2 MUTANTS. (2022): 37 - 43. 10.15671/hjbc.669854 |
| MLA | Erdem Tuncdemir Beril,Karaduman Tugce,Ozcan Turkmen Merve,Şahin Dilara,MERGEN HATICE,SAGLAR OZER EMEL CHEMICAL CHAPERONE EFFECTS ON ARGININE-VASOPRESSIN RECEPTOR 2 MUTANTS. , 2022, ss.37 - 43. 10.15671/hjbc.669854 |
| AMA | Erdem Tuncdemir B,Karaduman T,Ozcan Turkmen M,Şahin D,MERGEN H,SAGLAR OZER E CHEMICAL CHAPERONE EFFECTS ON ARGININE-VASOPRESSIN RECEPTOR 2 MUTANTS. . 2022; 37 - 43. 10.15671/hjbc.669854 |
| Vancouver | Erdem Tuncdemir B,Karaduman T,Ozcan Turkmen M,Şahin D,MERGEN H,SAGLAR OZER E CHEMICAL CHAPERONE EFFECTS ON ARGININE-VASOPRESSIN RECEPTOR 2 MUTANTS. . 2022; 37 - 43. 10.15671/hjbc.669854 |
| IEEE | Erdem Tuncdemir B,Karaduman T,Ozcan Turkmen M,Şahin D,MERGEN H,SAGLAR OZER E "CHEMICAL CHAPERONE EFFECTS ON ARGININE-VASOPRESSIN RECEPTOR 2 MUTANTS." , ss.37 - 43, 2022. 10.15671/hjbc.669854 |
| ISNAD | Erdem Tuncdemir, Beril vd. "CHEMICAL CHAPERONE EFFECTS ON ARGININE-VASOPRESSIN RECEPTOR 2 MUTANTS". (2022), 37-43. https://doi.org/10.15671/hjbc.669854 |
| APA | Erdem Tuncdemir B, Karaduman T, Ozcan Turkmen M, Şahin D, MERGEN H, SAGLAR OZER E (2022). CHEMICAL CHAPERONE EFFECTS ON ARGININE-VASOPRESSIN RECEPTOR 2 MUTANTS. Hacettepe Journal of Biology and Chemistry, 50(1), 37 - 43. 10.15671/hjbc.669854 |
| Chicago | Erdem Tuncdemir Beril,Karaduman Tugce,Ozcan Turkmen Merve,Şahin Dilara,MERGEN HATICE,SAGLAR OZER EMEL CHEMICAL CHAPERONE EFFECTS ON ARGININE-VASOPRESSIN RECEPTOR 2 MUTANTS. Hacettepe Journal of Biology and Chemistry 50, no.1 (2022): 37 - 43. 10.15671/hjbc.669854 |
| MLA | Erdem Tuncdemir Beril,Karaduman Tugce,Ozcan Turkmen Merve,Şahin Dilara,MERGEN HATICE,SAGLAR OZER EMEL CHEMICAL CHAPERONE EFFECTS ON ARGININE-VASOPRESSIN RECEPTOR 2 MUTANTS. Hacettepe Journal of Biology and Chemistry, vol.50, no.1, 2022, ss.37 - 43. 10.15671/hjbc.669854 |
| AMA | Erdem Tuncdemir B,Karaduman T,Ozcan Turkmen M,Şahin D,MERGEN H,SAGLAR OZER E CHEMICAL CHAPERONE EFFECTS ON ARGININE-VASOPRESSIN RECEPTOR 2 MUTANTS. Hacettepe Journal of Biology and Chemistry. 2022; 50(1): 37 - 43. 10.15671/hjbc.669854 |
| Vancouver | Erdem Tuncdemir B,Karaduman T,Ozcan Turkmen M,Şahin D,MERGEN H,SAGLAR OZER E CHEMICAL CHAPERONE EFFECTS ON ARGININE-VASOPRESSIN RECEPTOR 2 MUTANTS. Hacettepe Journal of Biology and Chemistry. 2022; 50(1): 37 - 43. 10.15671/hjbc.669854 |
| IEEE | Erdem Tuncdemir B,Karaduman T,Ozcan Turkmen M,Şahin D,MERGEN H,SAGLAR OZER E "CHEMICAL CHAPERONE EFFECTS ON ARGININE-VASOPRESSIN RECEPTOR 2 MUTANTS." Hacettepe Journal of Biology and Chemistry, 50, ss.37 - 43, 2022. 10.15671/hjbc.669854 |
| ISNAD | Erdem Tuncdemir, Beril vd. "CHEMICAL CHAPERONE EFFECTS ON ARGININE-VASOPRESSIN RECEPTOR 2 MUTANTS". Hacettepe Journal of Biology and Chemistry 50/1 (2022), 37-43. https://doi.org/10.15671/hjbc.669854 |
