TMEM106C is overexpressed and modulates cell mobility in metastatic colon cancer cells
Yıl: 2022 Cilt: 50 Sayı: 1 Sayfa Aralığı: 77 - 84 Metin Dili: İngilizce DOI: 10.15671/hjbc.892060 İndeks Tarihi: 29-07-2022
TMEM106C is overexpressed and modulates cell mobility in metastatic colon cancer cells
Öz: Colon cancer is the third most commonly diagnosed cancer in men worldwide. Colon cancer grows slowly and metastasis has already occurred after diagnosis. Therefore, new targets are needed in the colon cancer treatment and diagnosis. Transmembrane proteins (TMEM) play a critical role and presents different expression profile in variety of tumor cells. TMEM106C is a TMEM family protein, but its role on colon cancer development is unknown. In this study, we aimed to investigate TMEM106C gene in metastatic colon cancer cells. TMEM106C gene expression level was tested by western blot, qRT-PCR and immunofluorescence methods. In order to test the effect of TMEM106C in colon cancer cells, this gene has been knockdown with shRNA technology. In addition, cell invasion, migration and adhesion assays were performed to clarify whether TMEM106C knockdown has effect on colon cancer metastatic characters. Ford the first time, in this study, we showed TMEM106C is overexpressed in colon carcinoma cells. Moreover, we demonstrated that cell migration, invasion and adhesion capabilities are reduced in TMEM106C silenced cells. Furthermore, we observed that metastatic cell morphology was changed upon to TMEM106C knockdown. In conclusion, we showed that TMEM106C gene is important for colon carcinoma cells.
Anahtar Kelime: TMEM106C Metastatik Kolon Kanseri Hücrelerinde Aşırı Eksprese Olmuştur ve Hücre Hareketliliğini Modüle Eder
Öz: Kolon kanseri dünya çapında erkekler arasında teşhisi yapılan üçüncü kanser türüdür. Kolon kanseri gelişimi yavaştır ve teşhis konulduğunda metastaz gerçekleşmiştir. Bu nedenle kolon kanserinin teşhis ve tedavisinde yeni hedeflere ihtiyaç duyulmaktadır. Transmembran proteinleri (TMEM) farklı kanserlerin oluşmasında kritik rol oynamaktadır ve ifade seviyeleri çok farklılık göstermektedir. TMEM106C TMEM ailesi proteinlerden birisidir ancak kolon kanseri gelişim sürecindeki görevi bilinmemektedir. Bu çalışmada TMEM106C geninin metastatik kolon kanseri hücrelerindeki görevinin araştırılması amaçlanmıştır. TMEM106C gen ifadesi düzeyi western blot, qRT-PCR ve immünoflöresan yöntemleri ile test edilmiştir. TMEM106C’nin kolon kanseri hücrelerinde ki etkinliğinin test edilmesi için bu gen shRNA teknolojisi yardımıyla inaktive edilmiştir. Ayrıca TMEM106C inaktivasyonunun kolon kanseri metastatik karakterleri üzerine etkisini araştırmak için, hücre invazyon, göç ve adezyon testleri yapılmıştır. Çalışmada ilk defa TMEM106C’nin kolon karsinoma hücrelerinde aşırı ifade edildiği gösterilmiştir. Dahası TMEM106C inaktive edilmiş hücrelerde hücre göçü, invazyon ve adezyon yeteneklerinin azaldığı gösterilmiştir. Ayrıca TMEM106C gen inaktivasyonunun, metastatik hücre morfolojisini değiştirdiği gözlenmiştir. Sonuç olarak bu çalışma kapsamında TMEM106C geninin kolon karsinoma hücrelerindeki etkinliği gösterilmiştir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Dükel M (2022). TMEM106C is overexpressed and modulates cell mobility in metastatic colon cancer cells. , 77 - 84. 10.15671/hjbc.892060 |
| Chicago | Dükel Muzaffer TMEM106C is overexpressed and modulates cell mobility in metastatic colon cancer cells. (2022): 77 - 84. 10.15671/hjbc.892060 |
| MLA | Dükel Muzaffer TMEM106C is overexpressed and modulates cell mobility in metastatic colon cancer cells. , 2022, ss.77 - 84. 10.15671/hjbc.892060 |
| AMA | Dükel M TMEM106C is overexpressed and modulates cell mobility in metastatic colon cancer cells. . 2022; 77 - 84. 10.15671/hjbc.892060 |
| Vancouver | Dükel M TMEM106C is overexpressed and modulates cell mobility in metastatic colon cancer cells. . 2022; 77 - 84. 10.15671/hjbc.892060 |
| IEEE | Dükel M "TMEM106C is overexpressed and modulates cell mobility in metastatic colon cancer cells." , ss.77 - 84, 2022. 10.15671/hjbc.892060 |
| ISNAD | Dükel, Muzaffer. "TMEM106C is overexpressed and modulates cell mobility in metastatic colon cancer cells". (2022), 77-84. https://doi.org/10.15671/hjbc.892060 |
| APA | Dükel M (2022). TMEM106C is overexpressed and modulates cell mobility in metastatic colon cancer cells. Hacettepe Journal of Biology and Chemistry, 50(1), 77 - 84. 10.15671/hjbc.892060 |
| Chicago | Dükel Muzaffer TMEM106C is overexpressed and modulates cell mobility in metastatic colon cancer cells. Hacettepe Journal of Biology and Chemistry 50, no.1 (2022): 77 - 84. 10.15671/hjbc.892060 |
| MLA | Dükel Muzaffer TMEM106C is overexpressed and modulates cell mobility in metastatic colon cancer cells. Hacettepe Journal of Biology and Chemistry, vol.50, no.1, 2022, ss.77 - 84. 10.15671/hjbc.892060 |
| AMA | Dükel M TMEM106C is overexpressed and modulates cell mobility in metastatic colon cancer cells. Hacettepe Journal of Biology and Chemistry. 2022; 50(1): 77 - 84. 10.15671/hjbc.892060 |
| Vancouver | Dükel M TMEM106C is overexpressed and modulates cell mobility in metastatic colon cancer cells. Hacettepe Journal of Biology and Chemistry. 2022; 50(1): 77 - 84. 10.15671/hjbc.892060 |
| IEEE | Dükel M "TMEM106C is overexpressed and modulates cell mobility in metastatic colon cancer cells." Hacettepe Journal of Biology and Chemistry, 50, ss.77 - 84, 2022. 10.15671/hjbc.892060 |
| ISNAD | Dükel, Muzaffer. "TMEM106C is overexpressed and modulates cell mobility in metastatic colon cancer cells". Hacettepe Journal of Biology and Chemistry 50/1 (2022), 77-84. https://doi.org/10.15671/hjbc.892060 |
