Photoprotective and therapeutic effects of Apamin, Melittin and Phospholipase A2 on human keratinocyte cell line

ONUR, MEHMET; erfen, şebnem; AKBAY, ESİN; Özdemir, Alp Yiğit

doi:10.15671/hjbc.901013

Photoprotective and therapeutic effects of Apamin, Melittin and Phospholipase A2 on human keratinocyte cell line

ESİN ÇETİN, (Hacettepe Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Ankara, Türkiye)

Alp Yiğit ÖZDEMİR, (Hacettepe Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Ankara, Türkiye)

Şebnem ERFEN, (Hacettepe Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Ankara, Türkiye)

Mehmet Ali ONUR (Hacettepe Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Ankara, Türkiye)

Hacettepe Journal of Biology and Chemistry

14 0

Yıl: 2022 Cilt: 50 Sayı: 1 Sayfa Aralığı: 13 - 24 Metin Dili: İngilizce DOI: 10.15671/hjbc.901013 İndeks Tarihi: 29-07-2022

Photoprotective and therapeutic effects of Apamin, Melittin and Phospholipase A2 on human keratinocyte cell line

Öz:

The aim of this study is to determine the protective and therapeutic properties of bee venom components Apamin, Melittin and Phospholipase A2 against UV damage on the human keratinocyte cell line. Cosmetic and therapeutic effects of bee venom have been reported in previous studies, but it is not known which components of the venom have the most effect. For this purpose, firstly, an in vitro UV damage model was designed. Components of bee venom were tested at a different concentration. Cell viability, cytotoxicity and apoptotic processes were analyzed on the designed model. As a result, it has been observed that Apamin demonstrated a significant protective effect against UV-induced cell death at all concentrations. Photoprotective effects of Melittin were observed in all concentration time periods and its photoprotective effects were increased as the concentration increased. Our results were indicated that Phospholipase A2 could be used as a photoprotective and phototherapy agent. This study is the first to show the protective and therapeutic properties of bee venom components against UV damage. As a result, it has been shown that these components can be successful photoprotective and phototherapeutic agents against UV-induced damage, but more detailed studies should be conducted to minimize their toxic effects on cells.

Anahtar Kelime: Apamin

İnsan Keratinosit Hücre Hattında Apamin, Melittin ve Fosfalipaz A2’nin Fotokoruma ve Fototerapik Etkisi

Öz:

Bu çalışmanın amacı, arı zehiri bileşenleri olan Apamin, Melittin ve Fosfolipaz A2’nin insan keratinosit hücre hattındaki UV hasarına karşı koruyucu ve tedavi edici özelliklerini belirlemektir. Arı zehirinin kozmetik ve tedavi edici etkileri önceki çalışmalarda bildirilmiştir, ancak zehirin hangi bileşenlerinin en fazla etkiye sahip olduğu bilinmemektedir. Bu amaçla ilk olarak bir in vitro UV hasar modeli tasarlandı. Arı zehirinin bileşenleri farklı konsantrasyonlarda test edildi. Hücre canlılığı, sitotoksisite ve apoptotik süreçler tasarlanan model üzerinde analiz edildi. Sonuç olarak, Apamin’in tüm konsantrasyonlarda UV ile indüklenen hücre ölümüne karşı önemli bir koruyucu etki gösterdiği gözlendi. Melittin’in ışık koruyucu etkileri tüm konsantrasyon sürelerinde gözlendi ve yüksek konsantrasyonda ışık koruyucu etkilerinin arttığı tespit edildi. Sonuçlarımız, Fosfolipaz A2’nin bir fotokoruyucu ve fototerapi ajanı olarak kullanılabileceğini gösterdi. Bu çalışma, arı zehiri bileşenlerinin UV hasarına karşı koruyucu ve tedavi edici özelliklerini gösteren ilk çalışmadır. Sonuç olarak, bu bileşenlerin UV kaynaklı hasara karşı başarılı fotokoruyucu ve fototerapötik ajanlar olabileceği gösterilmiştir, ancak hücreler üzerindeki toksik etkilerini en aza indirmek için daha detaylı çalışmalar yapılmalıdır.

Anahtar Kelime: Apamin

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	AKBAY E, Özdemir A, erfen ş, ONUR M (2022). Photoprotective and therapeutic effects of Apamin, Melittin and Phospholipase A2 on human keratinocyte cell line. , 13 - 24. 10.15671/hjbc.901013
Chicago	AKBAY ESİN,Özdemir Alp Yiğit,erfen şebnem,ONUR MEHMET Photoprotective and therapeutic effects of Apamin, Melittin and Phospholipase A2 on human keratinocyte cell line. (2022): 13 - 24. 10.15671/hjbc.901013
MLA	AKBAY ESİN,Özdemir Alp Yiğit,erfen şebnem,ONUR MEHMET Photoprotective and therapeutic effects of Apamin, Melittin and Phospholipase A2 on human keratinocyte cell line. , 2022, ss.13 - 24. 10.15671/hjbc.901013
AMA	AKBAY E,Özdemir A,erfen ş,ONUR M Photoprotective and therapeutic effects of Apamin, Melittin and Phospholipase A2 on human keratinocyte cell line. . 2022; 13 - 24. 10.15671/hjbc.901013
Vancouver	AKBAY E,Özdemir A,erfen ş,ONUR M Photoprotective and therapeutic effects of Apamin, Melittin and Phospholipase A2 on human keratinocyte cell line. . 2022; 13 - 24. 10.15671/hjbc.901013
IEEE	AKBAY E,Özdemir A,erfen ş,ONUR M "Photoprotective and therapeutic effects of Apamin, Melittin and Phospholipase A2 on human keratinocyte cell line." , ss.13 - 24, 2022. 10.15671/hjbc.901013
ISNAD	AKBAY, ESİN vd. "Photoprotective and therapeutic effects of Apamin, Melittin and Phospholipase A2 on human keratinocyte cell line". (2022), 13-24. https://doi.org/10.15671/hjbc.901013

APA	AKBAY E, Özdemir A, erfen ş, ONUR M (2022). Photoprotective and therapeutic effects of Apamin, Melittin and Phospholipase A2 on human keratinocyte cell line. Hacettepe Journal of Biology and Chemistry, 50(1), 13 - 24. 10.15671/hjbc.901013
Chicago	AKBAY ESİN,Özdemir Alp Yiğit,erfen şebnem,ONUR MEHMET Photoprotective and therapeutic effects of Apamin, Melittin and Phospholipase A2 on human keratinocyte cell line. Hacettepe Journal of Biology and Chemistry 50, no.1 (2022): 13 - 24. 10.15671/hjbc.901013
MLA	AKBAY ESİN,Özdemir Alp Yiğit,erfen şebnem,ONUR MEHMET Photoprotective and therapeutic effects of Apamin, Melittin and Phospholipase A2 on human keratinocyte cell line. Hacettepe Journal of Biology and Chemistry, vol.50, no.1, 2022, ss.13 - 24. 10.15671/hjbc.901013
AMA	AKBAY E,Özdemir A,erfen ş,ONUR M Photoprotective and therapeutic effects of Apamin, Melittin and Phospholipase A2 on human keratinocyte cell line. Hacettepe Journal of Biology and Chemistry. 2022; 50(1): 13 - 24. 10.15671/hjbc.901013
Vancouver	AKBAY E,Özdemir A,erfen ş,ONUR M Photoprotective and therapeutic effects of Apamin, Melittin and Phospholipase A2 on human keratinocyte cell line. Hacettepe Journal of Biology and Chemistry. 2022; 50(1): 13 - 24. 10.15671/hjbc.901013
IEEE	AKBAY E,Özdemir A,erfen ş,ONUR M "Photoprotective and therapeutic effects of Apamin, Melittin and Phospholipase A2 on human keratinocyte cell line." Hacettepe Journal of Biology and Chemistry, 50, ss.13 - 24, 2022. 10.15671/hjbc.901013
ISNAD	AKBAY, ESİN vd. "Photoprotective and therapeutic effects of Apamin, Melittin and Phospholipase A2 on human keratinocyte cell line". Hacettepe Journal of Biology and Chemistry 50/1 (2022), 13-24. https://doi.org/10.15671/hjbc.901013