Effect of alpha-tocopherol and dose sensitivity on pancreatitis formation in rats with experimental pancreatitis
Yıl: 2021 Cilt: 27 Sayı: 6 Sayfa Aralığı: 606 - 612 Metin Dili: İngilizce DOI: 10.14744/tjtes.2020.89054 İndeks Tarihi: 18-05-2022
Effect of alpha-tocopherol and dose sensitivity on pancreatitis formation in rats with experimental pancreatitis
Öz: BACKGROUND: Acute pancreatitis is an inflammatory disease accompanied by pancreatic inflammation characterized by acinar cell damage and leukocyte infiltration in the tissue. At present, mortality and morbidity rates are high despite the current treatment of pancreatitis; therefore, new studies and treatment studies are needed. In this study, the effects of alpha-tocopherol on different doses of L-arginine-induced experimental acute pancreatitis model were investigated. METHODS: Thirty adult male Sprague–Dawley albino rats were randomly divided into four groups; control (sham) group (n=6), acute pancreatitis group (n=8), low-dose alpha-tocopherol (200 mg/kg once intraperitoneal [IP]) group (n=8), and high dose alpha-tocopherol (400 mg/kg once ip) group (n=8). Experimental acute pancreatitis model was created by a single IP dose of 5 g/kg of L-arginine. Alpha-tocopherol was administered in a single dose intraperitoneally, 30 min before the creation of the experimental model of acute pancreatitis induced by L-arginine induction in Groups 3 and 4. Tissue and blood samples were taken under anesthesia 72 h after L-arginine injection; then the rats were sacrificed by decapitation. Serum amylase, lipase, interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-alpha, and C-reactive protein (CRP) levels were examined. Pancreatic tissue samples were examined under a light microscope for histopathological examination. RESULTS: When the acute pancreatitis group (Group 2) was compared to the control group (Group 1), serum amylase, lipase, IL1β, IL-6, TNF-alpha, and CRP levels were all significantly increased (p<0.05 for all). Histopathological examination showed significant difference in edema (p<0.001) and inflammation (p=0.007) scores. When the low (Group 3) and high (Group 4) dose alpha-tocopherol groups were compared to Group 2, amylase, lipase, IL-1β, IL-6, TNF-alpha, and CRP parameters were statistically significantly lower (p<0.05 for all). In the histopathological comparison of Groups 2, 3, and 4, edema and inflammation scores were decreased in Groups 3 and 4 compared to Group 2. Comparing Group 4 to Group 3, lipase (p<0.01), IL-6 (p=0.038), and TNF-alpha (p=0.002) levels were significantly decreased; no significant difference was observed in the histopathological evaluation. CONCLUSION: Alpha-tocopherol was found to reduce inflammation and pancreatic damage in acute pancreatitis and was more effective in high doses
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| APA | Tazeoglu D, Akyüz C, Gökçeimam M, harman kamali g, ÖZSOY A, Karahan S (2021). Effect of alpha-tocopherol and dose sensitivity on pancreatitis formation in rats with experimental pancreatitis. , 606 - 612. 10.14744/tjtes.2020.89054 |
| Chicago | Tazeoglu Deniz,Akyüz Cüneyt,Gökçeimam Mehmet,harman kamali gulcin,ÖZSOY AYHAN,Karahan Servet Rüştü Effect of alpha-tocopherol and dose sensitivity on pancreatitis formation in rats with experimental pancreatitis. (2021): 606 - 612. 10.14744/tjtes.2020.89054 |
| MLA | Tazeoglu Deniz,Akyüz Cüneyt,Gökçeimam Mehmet,harman kamali gulcin,ÖZSOY AYHAN,Karahan Servet Rüştü Effect of alpha-tocopherol and dose sensitivity on pancreatitis formation in rats with experimental pancreatitis. , 2021, ss.606 - 612. 10.14744/tjtes.2020.89054 |
| AMA | Tazeoglu D,Akyüz C,Gökçeimam M,harman kamali g,ÖZSOY A,Karahan S Effect of alpha-tocopherol and dose sensitivity on pancreatitis formation in rats with experimental pancreatitis. . 2021; 606 - 612. 10.14744/tjtes.2020.89054 |
| Vancouver | Tazeoglu D,Akyüz C,Gökçeimam M,harman kamali g,ÖZSOY A,Karahan S Effect of alpha-tocopherol and dose sensitivity on pancreatitis formation in rats with experimental pancreatitis. . 2021; 606 - 612. 10.14744/tjtes.2020.89054 |
| IEEE | Tazeoglu D,Akyüz C,Gökçeimam M,harman kamali g,ÖZSOY A,Karahan S "Effect of alpha-tocopherol and dose sensitivity on pancreatitis formation in rats with experimental pancreatitis." , ss.606 - 612, 2021. 10.14744/tjtes.2020.89054 |
| ISNAD | Tazeoglu, Deniz vd. "Effect of alpha-tocopherol and dose sensitivity on pancreatitis formation in rats with experimental pancreatitis". (2021), 606-612. https://doi.org/10.14744/tjtes.2020.89054 |
| APA | Tazeoglu D, Akyüz C, Gökçeimam M, harman kamali g, ÖZSOY A, Karahan S (2021). Effect of alpha-tocopherol and dose sensitivity on pancreatitis formation in rats with experimental pancreatitis. Ulusal Travma ve Acil Cerrahi Dergisi, 27(6), 606 - 612. 10.14744/tjtes.2020.89054 |
| Chicago | Tazeoglu Deniz,Akyüz Cüneyt,Gökçeimam Mehmet,harman kamali gulcin,ÖZSOY AYHAN,Karahan Servet Rüştü Effect of alpha-tocopherol and dose sensitivity on pancreatitis formation in rats with experimental pancreatitis. Ulusal Travma ve Acil Cerrahi Dergisi 27, no.6 (2021): 606 - 612. 10.14744/tjtes.2020.89054 |
| MLA | Tazeoglu Deniz,Akyüz Cüneyt,Gökçeimam Mehmet,harman kamali gulcin,ÖZSOY AYHAN,Karahan Servet Rüştü Effect of alpha-tocopherol and dose sensitivity on pancreatitis formation in rats with experimental pancreatitis. Ulusal Travma ve Acil Cerrahi Dergisi, vol.27, no.6, 2021, ss.606 - 612. 10.14744/tjtes.2020.89054 |
| AMA | Tazeoglu D,Akyüz C,Gökçeimam M,harman kamali g,ÖZSOY A,Karahan S Effect of alpha-tocopherol and dose sensitivity on pancreatitis formation in rats with experimental pancreatitis. Ulusal Travma ve Acil Cerrahi Dergisi. 2021; 27(6): 606 - 612. 10.14744/tjtes.2020.89054 |
| Vancouver | Tazeoglu D,Akyüz C,Gökçeimam M,harman kamali g,ÖZSOY A,Karahan S Effect of alpha-tocopherol and dose sensitivity on pancreatitis formation in rats with experimental pancreatitis. Ulusal Travma ve Acil Cerrahi Dergisi. 2021; 27(6): 606 - 612. 10.14744/tjtes.2020.89054 |
| IEEE | Tazeoglu D,Akyüz C,Gökçeimam M,harman kamali g,ÖZSOY A,Karahan S "Effect of alpha-tocopherol and dose sensitivity on pancreatitis formation in rats with experimental pancreatitis." Ulusal Travma ve Acil Cerrahi Dergisi, 27, ss.606 - 612, 2021. 10.14744/tjtes.2020.89054 |
| ISNAD | Tazeoglu, Deniz vd. "Effect of alpha-tocopherol and dose sensitivity on pancreatitis formation in rats with experimental pancreatitis". Ulusal Travma ve Acil Cerrahi Dergisi 27/6 (2021), 606-612. https://doi.org/10.14744/tjtes.2020.89054 |
