Molecular detection of E. faecalis in oral samples of a
population associated with secondary endodontic
infection

ASLAN, GÖNÜL; Kucuk, Mustafa Oner; Aslan, Tugrul; ERSOY, LEYLA; KESİM, Bertan; Tezcan Ülger, Seda; CUDAL, Hamza

doi:10.5577/intdentres.2021.vol11.no3.7

Molecular detection of E. faecalis in oral samples of a population associated with secondary endodontic infection

Bertan KESİM, (Nuh Naci Yazgan University, Faculty of Dentistry, Department of Endodontics, Kayseri, Turkey)

Seda Tezcan ÜLGER, (Mersin University, Faculty of Medicine, Department of Medical Microbiology, Mersin, Turkey)

Hamza CUDAL, (Nuh Naci Yazgan University, Faculty of Dentistry, Department of Endodontics, Kayseri, Turkey)

Gönül ASLAN, (Mersin University, Faculty of Medicine, Department of Medical Microbiology, Mersin, Turkey)

Leyla ERSOY, (Mersin University, Faculty of Medicine, Department of Medical Microbiology, Mersin, Turkey)

Tuğrul ARSLAN, (Erciyes University, Faculty of Dentistry, Department of Endodontics, Kayseri, Turkey)

Mustafa Öner KÜÇÜK (Nuh Naci Yazgan University, Faculty of Dentistry, Department of Microbiology, Kayseri, Turkey)

International Dental Research

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Yıl: 2021 Cilt: 11 Sayı: 3 Sayfa Aralığı: 180 - 184 Metin Dili: İngilizce DOI: 10.5577/intdentres.2021.vol11.no3.7 İndeks Tarihi: 25-05-2022

Molecular detection of E. faecalis in oral samples of a population associated with secondary endodontic infection

Öz:

Aim: The objective of this study was to evaluate the prevalence of Enterococcus faecalis in samples of oral rinse and tongue dorsum of endodontic patients with secondary/persistent infections (EPSI) using the PCR method. Methodology: Oral rinse samples (ORS) and tongue swab samples (TSS) of 22 patients (EPSI group) and 32 healthy individuals (control group) were collected. DNA isolation from the TSS and ORS samples was performed using the modified classical phenol-chloroform and chloroform method. To detect E. faecalis strains directly from the TSS and ORS samples, the 310 base pair (bp) segment of the 16S rDNA of the E. faecalis genome was amplified by PCR using specific primers. The prevalence of E. faecalis was compared between healthy and sick individuals using the Chi-square test, significance was set at p<0.05. Results: In the ORS samples, there was a significant difference between the healthy individuals (n = 11, 34%) and the EPSI group (n = 15, 68%) in terms of the presence of E. faecalis (p = 0.026). In the TSS, the presence of E. faecalis was also investigated, and a significant difference was found between healthy individuals (n = 3, 9%) and the EPSI group (n = 11, 50%) (p = 0.001). In the EPSI group, no statistically significant difference was present in the prevalence rate of E. faecalis between the samples of ORS (68%) and TSS (50%) (p = 0.358). Conclusion: The prevalence of E. faecalis was found to be statistically significantly higher in multi-site oral samples of a population with secondary endodontic infection than healthy individuals.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	KESİM B, Tezcan Ülger S, CUDAL H, ASLAN G, ERSOY L, Aslan T, Kucuk M (2021). Molecular detection of E. faecalis in oral samples of a population associated with secondary endodontic infection. , 180 - 184. 10.5577/intdentres.2021.vol11.no3.7
Chicago	KESİM Bertan,Tezcan Ülger Seda,CUDAL Hamza,ASLAN GÖNÜL,ERSOY LEYLA,Aslan Tugrul,Kucuk Mustafa Oner Molecular detection of E. faecalis in oral samples of a population associated with secondary endodontic infection. (2021): 180 - 184. 10.5577/intdentres.2021.vol11.no3.7
MLA	KESİM Bertan,Tezcan Ülger Seda,CUDAL Hamza,ASLAN GÖNÜL,ERSOY LEYLA,Aslan Tugrul,Kucuk Mustafa Oner Molecular detection of E. faecalis in oral samples of a population associated with secondary endodontic infection. , 2021, ss.180 - 184. 10.5577/intdentres.2021.vol11.no3.7
AMA	KESİM B,Tezcan Ülger S,CUDAL H,ASLAN G,ERSOY L,Aslan T,Kucuk M Molecular detection of E. faecalis in oral samples of a population associated with secondary endodontic infection. . 2021; 180 - 184. 10.5577/intdentres.2021.vol11.no3.7
Vancouver	KESİM B,Tezcan Ülger S,CUDAL H,ASLAN G,ERSOY L,Aslan T,Kucuk M Molecular detection of E. faecalis in oral samples of a population associated with secondary endodontic infection. . 2021; 180 - 184. 10.5577/intdentres.2021.vol11.no3.7
IEEE	KESİM B,Tezcan Ülger S,CUDAL H,ASLAN G,ERSOY L,Aslan T,Kucuk M "Molecular detection of E. faecalis in oral samples of a population associated with secondary endodontic infection." , ss.180 - 184, 2021. 10.5577/intdentres.2021.vol11.no3.7
ISNAD	KESİM, Bertan vd. "Molecular detection of E. faecalis in oral samples of a population associated with secondary endodontic infection". (2021), 180-184. https://doi.org/10.5577/intdentres.2021.vol11.no3.7

APA	KESİM B, Tezcan Ülger S, CUDAL H, ASLAN G, ERSOY L, Aslan T, Kucuk M (2021). Molecular detection of E. faecalis in oral samples of a population associated with secondary endodontic infection. International Dental Research, 11(3), 180 - 184. 10.5577/intdentres.2021.vol11.no3.7
Chicago	KESİM Bertan,Tezcan Ülger Seda,CUDAL Hamza,ASLAN GÖNÜL,ERSOY LEYLA,Aslan Tugrul,Kucuk Mustafa Oner Molecular detection of E. faecalis in oral samples of a population associated with secondary endodontic infection. International Dental Research 11, no.3 (2021): 180 - 184. 10.5577/intdentres.2021.vol11.no3.7
MLA	KESİM Bertan,Tezcan Ülger Seda,CUDAL Hamza,ASLAN GÖNÜL,ERSOY LEYLA,Aslan Tugrul,Kucuk Mustafa Oner Molecular detection of E. faecalis in oral samples of a population associated with secondary endodontic infection. International Dental Research, vol.11, no.3, 2021, ss.180 - 184. 10.5577/intdentres.2021.vol11.no3.7
AMA	KESİM B,Tezcan Ülger S,CUDAL H,ASLAN G,ERSOY L,Aslan T,Kucuk M Molecular detection of E. faecalis in oral samples of a population associated with secondary endodontic infection. International Dental Research. 2021; 11(3): 180 - 184. 10.5577/intdentres.2021.vol11.no3.7
Vancouver	KESİM B,Tezcan Ülger S,CUDAL H,ASLAN G,ERSOY L,Aslan T,Kucuk M Molecular detection of E. faecalis in oral samples of a population associated with secondary endodontic infection. International Dental Research. 2021; 11(3): 180 - 184. 10.5577/intdentres.2021.vol11.no3.7
IEEE	KESİM B,Tezcan Ülger S,CUDAL H,ASLAN G,ERSOY L,Aslan T,Kucuk M "Molecular detection of E. faecalis in oral samples of a population associated with secondary endodontic infection." International Dental Research, 11, ss.180 - 184, 2021. 10.5577/intdentres.2021.vol11.no3.7
ISNAD	KESİM, Bertan vd. "Molecular detection of E. faecalis in oral samples of a population associated with secondary endodontic infection". International Dental Research 11/3 (2021), 180-184. https://doi.org/10.5577/intdentres.2021.vol11.no3.7