The Effect of Age and Gender on Some Hematological Parameters and Erythrocyte Osmotic Fragility in Kangal Shepherd Dogs

BAŞ EKİCİ, Hacer; KOÇKAYA, Mustafa; Genç, Engin; Çelikgün, Serkan; EKICI, MEHMET

doi:10.17094/ataunivbd.1051477

The Effect of Age and Gender on Some Hematological Parameters and Erythrocyte Osmotic Fragility in Kangal Shepherd Dogs

Mustafa KOÇKAYA, (Sivas Cumhuriyet Üniversitesi, Veteriner Fakültesi, Fizyoloji Anabilim Dalı, Sivas, Türkiye)

Mehmet EKİCİ, (Sivas Cumhuriyet Üniversitesi, Veteriner Fakültesi, Fizyoloji Anabilim Dalı, Sivas, Türkiye)

Engin GENÇ, (Kangal Kaymakamlığı, Sivas, Türkiye)

Serkan ÇELİKGÜN, (Dokuz Eylül Üniversitesi, Sağlık Bilimleri Enstitüsü, Halk Sağlığı Anabilim Dalı, İzmir, Türkiye)

Hacer BAŞ EKİCİ (Selçuk Üniversitesi, Veteriner Fakültesi, Anatomi Anabilim Dalı, Konya, Türkiye)

Atatürk Üniversitesi Veteriner Bilimleri Dergisi

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Yıl: 2021 Cilt: 16 Sayı: 3 Sayfa Aralığı: 298 - 305 Metin Dili: İngilizce DOI: 10.17094/ataunivbd.1051477 İndeks Tarihi: 29-07-2022

The Effect of Age and Gender on Some Hematological Parameters and Erythrocyte Osmotic Fragility in Kangal Shepherd Dogs

Öz:

This study was aimed to determine the effect of age and gender on physiological variations in some hematological parameters and erythrocyte osmotic fragility in Kangal Shepherd dogs. A total of 32 clinically healthy Kangal Shepherd dogs were taken into the study and divided into 4 groups in equal numbers (Adult male = 8, Adult female = 8, Senior male = 8, Senior female = 8). Blood samples from the cephalic vein were taken into vacuum tubes containing EDTA by the technique and values of erythrocyte osmotic fragility and complete blood count were determined. The main effect of age and gender with age x gender interaction has no significant effect on hematological parameters (P>0.05). The main effect of age and age x gender interaction of erythrocyte osmotic fragility was statistically significant and higher in the senior females at 0.7% and 0.9% (in addition gender) NaCl concentrations compared to the other groups (P<0.05). However, there was no statistical difference between the adult female and adult male and senior male group at 0.7% and 0.9% NaCl concentrations (P>0.05). The findings may be useful for veterinary clinical practice in establishing Kangal shepherd breed-specific reference ranges for some important hematological parameters.

Anahtar Kelime: Hematology

Kangal Çoban Köpeklerinde Yaş ve Cinsiyetin Bazı Hematolojik Parametreler ve Eritrosit Ozmotik Kırılganlık Üzerine Etkisi

Öz:

Bu çalışmada, Kangal çoban köpeklerinde yaş ve cinsiyetin bazı hematolojik parametrelerdeki fizyolojik varyasyonlar ve eritrosit ozmotik kırılganlık üzerindeki etkisinin belirlenmesi amaçlanmıştır. Çalışmaya klinik olarak sağlıklı toplam 32 Kangal çoban köpeği alınmış ve Kangal çoban köpekleri eşit sayıda 4 gruba ayrılmıştır (Yetişkin erkek Kangal çoban köpekleri = 8, Yetişkin dişi Kangal çoban köpekleri= 8, Yaşlı erkek Kangal çoban köpekleri = 8, Yaşlı dişi Kangal çoban köpekleri = 8). Sefalik venden kan örnekleri, tekniğine uygun olarak EDTA içeren vakum tüplerine alındı ve eritrosit ozmotik kırılganlık ile tam kan sayımı değerleri belirlendi. Hematolojik parametreler üzerine yaş ve cinsiyetin ana etkisi ile yaş x cinsiyet etkileşiminin anlamlı bir etkisi yoktu (P>0.05). Eritrosit ozmotik kırılganlığında yaş x cinsiyet etkileşimi ile yaşın ana etkisi, diğer gruplara kıyasla %0.7 ve %0.9 (ek olarak cinsiyet) NaCl konsantrasyonlarında yaşlı dişilerde istatistiksel olarak anlamlı ve daha yüksekti (P<0.05). Bununla birlikte, yetişkin dişi köpekler ve yetişkin erkek köpekler ile yaşlı erkek köpek grubu arasında %0.7 ve %0.9 NaCl konsantrasyonlarda istatistiksel olarak bir fark bulunmadı (P>0.05). Elde edilen bulgular, bazı önemli hematolojik parametreler için Kangal çoban köpek ırkına spesifik referans aralıkları oluşturmada veteriner klinik uygulamalara yararlı olabilecektir.

Anahtar Kelime: Hematoloji Yaş Eritrosit ozmotik kırılganlık Cinsiyet kangal çoban köpekleri

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Koçkaya M., Özşensoy Y., İnsal B., 2019. Comparisons of some physiological and stress behavioral parameters of Kangal shepherd dogs with and without ankyloglossia in different environmental temperatures. Turk J Vet Anim Sci, 43, 314-322.
2. Koçkaya M., Eki̇ci̇ M., 2020. The effect of acute strenuous exercise on some physiological, blood and antioxidant system parameters in Kangal shepherd dogs with and without ankyloglossia. MAE Vet Fak Derg, 5, 100-105.
3. Insal B., Piskin İ., 2020. Determination of some coagulation parameters according to age and sex in Sivas Kangal dogs. Turk J Vet Anim Sci, 44, 214-219.
4. Durgun Z., Eksen M., Keskin E., 1993. Some hematological values in healthy Kangal and German wolf dogs. Selcuk Univ Vet Fak Derg, 9, 16-20.
5. Koçkaya M., 2019. Comparisons of some blood hematological levels and biochemical parameters in pregnant and non-pregnant Kangal shepherd dogs. Int J Vet Sci Anim Husb, 4, 5-8.
6. Souza KL., Falbo MK., 2020. Effect of time and storage temperature on hematological parameters of healthy dogs. PUBVET, 14, 157-166.
7. Lee HS., Kim JH., 2020. The dog as an exercise science animal model: a review of physiological and hematological effects of exercise conditions. Phys Act Nutr, 24, 1-6.
8. Oyewale JO., Durotoye LA, 1988. Osmotic fragility of erythrocytes of two breeds of domestic fowl in the warm humid tropics. Lab Anim, 22, 250-254.
9. Lektib I., Bargaa R., Chakir Y., Belhouari A., Hammoumi A., El Khasmi M., 2016. Study of incubation conditions for erythrocytes osmotic fragility testing in dromedary camel (Camelus dromedarius). Int J Res Env Sci, 2, 22-32.
10. Wu SG., Jeng FR., Wei SY., Su CZ., Chung TC., Chang WJ., Chang HW., 1998. Red blood cell osmotic fragility in chronically hemodialyzed patients. Nephron, 78, 28-32.
11. Adenkola AY., Ayo JO., Sackey AKB., Adelaiye AB., 2010. Erythrocyte osmotic fragility of pigs administered ascorbic acid and transported by road for short term duration during the harmatan season. Afr J Biotechnol, 9, 226.
12. Oladele SB., Ayo JO., Ogundipe SO., Esieo KAN., 2003. Seasonal and species variations in erythrocytes osmotic fragility of indigenous poultry species in Zaria, Northern guinea savannah zone of Nigeria. Bull Anim Hlth Prod Afr, 51, 204-214.
13. Ambali SF., Abubakar AT., Shittu M., Yaqub LS., Kobo PI., Giwa A., 2010. Ameliorative effect of zinc on chlorpyrifos-induced erythrocyte fragility in Wistar rats. New York Sci J, 3, 117-122.
14. Igbokwe NA., Ojo NA., Igbokwe IO., 2016. Effects of sex and age on the osmotic stability of Sahel goat erythrocytes. Com Clin Path, 25, 15-22.
15. Mosior M., Gomulkıewicz J., 1988. Osmotic properties of bovine erythrocytes aged in vivo. Gen Physiol Biophys, 79, 73-79.
16. Rifkind JM., Araki K., Hadley EC., 1983. The relationship between the osmotic fragility of human erythrocytes and cell age. Arch Biochem Biophys, 222, 582-589.
17. Mosior M., Gomulkiewicz J., 1985. Effect of phosphate ions on osmotic properties of human and bovine erythrocytes-a relation between the state of glycolysis and critical cell-volume. Studia Biophysica, 107, 169-178.
18. Perez JM., Gonzalez FJ., Granados JE., Perez MC., Fandos P., Soriguer RC., Serrano E., 2003. Hematologic and biochemical reference intervals for Spanish ibex. J Wildl Dis, 39, 209-215.
19. Meyer DJ., Harvey JW. Veterinary laboratory medicine, 3rd edn. Saunders, St Louis, 2004.
20. Fortney WD., 2012. Implementing a successful senior/geriatric health care program for veterinarians, veterinary technicians, and office managers. Vet Clin North Am Small Anim Pract, 42, 823-834.
21. Olayemi FO., Azeez IO., Ogunyemi A., Ighagbon FO., 2009. Study on erythrocyte values of the Nigerian indigenous dog. Folia Veterinaria, 53, 65-67.
22. Ackermann M., Chao L., Bergstrom CT., Doebeli M., 2007. On the evolutionary origin of aging. Aging Cell, 6, 235-244.
23. Lopez-Otin C., Blasco MA., Partridge L., Serrano M., Kroemer G., 2013. The hallmarks of aging. Cell, 153, 1194-1217.
24. Metzger FL., Rebar AH., 2012. Clinical pathology interpretation in geriatric veterinary patients. Vet Clin North Am Small Anim Pract, 42, 615-629.
25. Harper EJ., Hackett RM., Wilkinson J., Heaton PR., 2003. Age-related variations in hematologic and plasma biochemical test results in Beagles and Labrador Retrievers. J Am Vet Med Assoc, 223, 1436-1442.
26. Lawrence J., Chang YM., Szladovits B., Davison LJ., Garden OA., 2013. Breed-specific hematological phenotypes in the dog: a natural resource for the genetic dissection of hematological parameters in a mammalian species. PLoS One, 8, e81288.
27. Lee SH., Kim JW., Lee BC., Oh HJ., 2020. Age-specific variations in hematological and biochemical parameters in middle-and large-sized of dogs. J Vet Sci, 21, e7.
28. Doğan A., 2020. Diyabetik ratlarda zakkum(nerium oleander L.) çiçeği etanolik liyofilize ekstresinin eritrosit frajilite, hematolojik ve antioksidan etkilerinin araştırılması. KSÜ Tarım ve Doğa Derg, 23, 1495-1502.
29. Pati S., Panda SK., Behera PC., Panda MR., 2017. Assessment of erythrocyte osmotic fragility in cattle due to haemoprotozoan diseases. Int J Sci Environ Technol, 6, 1560-1568.
30. Igbokwe NA., 2018. A review of the factors that influence erythrocyte osmotic fragility. Sokoto J of Vet Sci, 16, 1-23.
31. Marin MS., Fernandez A., Sanchez-Yaque J., Cabezas JA., Lianillo M., 1990. Changes in the phospholipid and fatty acid composittion in normal erythrocytes from sheep of different ages. Aminophospholoipid organisation in the membrane bilayer. Biochimie, 72, 745-750.
32. Kumar A., 2011. Biomedical studies on lipid peroxidation and erythrocyte fragility during the process of aging. Asian Pac J Trop Biomed, 1, 6-7.
33. Tiffert T., Daw N., Etzion Z., Bookchin RM., Lew VL., 2007. Age decline in the activity of the Ca2+-sensitive K+ channel of human red blood cells. J Gen Physiol, 129, 429-436.
34. Oyewale JO., 1988. Osmotic fragility of erythrocytes of guinea-fowls at 21 and 156 weeks of age. Vet Arhiv, 61, 49-56.
35. Basarab JA., Berg RT., Thompson JR., 1980. Erythrocyte fragility in double-muscled cattle. Can J Anim Sci, 60, 869-876.
36. Oyewale JO., Ajibade HA., 1990. Osmotic fragility of erythrocytes of the White Pekin duck. Vet Arhiv, 60, 91-100.
37. Olayemi FO., 2007. The effect of sex on the erythrocyte osmotic fragility of the Nigerian White Fulani and Ndama breeds of cattle. Top Vet 25, 106-111.
38. Durotoye LA., 1987. The effect of sex, pregnancy and lactation on the osmotic fragility of the West African dwarf sheep. Bull Anim Hlth Prod Afr, 35, 29-33.
39. Oyewale JO., Olayemi FO., Oke OA., 1998. Haematology of the wild adult African giant rat (Cricetomys gambianus, waterhouse). Vet Arhiv, 68, 91-99.
40. Habibu B., Kawu MU., Makun HJ., Aluwong T., Yaqub LS., Ahmad MS., Tauheed M., Buhari HU., 2014. Influence of sex, reproductive statusand foetal number on erythrocyte osmotic fragility, haematological and physiological parameters in goats during the hot-dry season. Vet Med, 59, 479-490.

APA	KOÇKAYA M, EKICI M, Genç E, Çelikgün S, BAŞ EKİCİ H (2021). The Effect of Age and Gender on Some Hematological Parameters and Erythrocyte Osmotic Fragility in Kangal Shepherd Dogs. , 298 - 305. 10.17094/ataunivbd.1051477
Chicago	KOÇKAYA Mustafa,EKICI MEHMET,Genç Engin,Çelikgün Serkan,BAŞ EKİCİ Hacer The Effect of Age and Gender on Some Hematological Parameters and Erythrocyte Osmotic Fragility in Kangal Shepherd Dogs. (2021): 298 - 305. 10.17094/ataunivbd.1051477
MLA	KOÇKAYA Mustafa,EKICI MEHMET,Genç Engin,Çelikgün Serkan,BAŞ EKİCİ Hacer The Effect of Age and Gender on Some Hematological Parameters and Erythrocyte Osmotic Fragility in Kangal Shepherd Dogs. , 2021, ss.298 - 305. 10.17094/ataunivbd.1051477
AMA	KOÇKAYA M,EKICI M,Genç E,Çelikgün S,BAŞ EKİCİ H The Effect of Age and Gender on Some Hematological Parameters and Erythrocyte Osmotic Fragility in Kangal Shepherd Dogs. . 2021; 298 - 305. 10.17094/ataunivbd.1051477
Vancouver	KOÇKAYA M,EKICI M,Genç E,Çelikgün S,BAŞ EKİCİ H The Effect of Age and Gender on Some Hematological Parameters and Erythrocyte Osmotic Fragility in Kangal Shepherd Dogs. . 2021; 298 - 305. 10.17094/ataunivbd.1051477
IEEE	KOÇKAYA M,EKICI M,Genç E,Çelikgün S,BAŞ EKİCİ H "The Effect of Age and Gender on Some Hematological Parameters and Erythrocyte Osmotic Fragility in Kangal Shepherd Dogs." , ss.298 - 305, 2021. 10.17094/ataunivbd.1051477
ISNAD	KOÇKAYA, Mustafa vd. "The Effect of Age and Gender on Some Hematological Parameters and Erythrocyte Osmotic Fragility in Kangal Shepherd Dogs". (2021), 298-305. https://doi.org/10.17094/ataunivbd.1051477

APA	KOÇKAYA M, EKICI M, Genç E, Çelikgün S, BAŞ EKİCİ H (2021). The Effect of Age and Gender on Some Hematological Parameters and Erythrocyte Osmotic Fragility in Kangal Shepherd Dogs. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 16(3), 298 - 305. 10.17094/ataunivbd.1051477
Chicago	KOÇKAYA Mustafa,EKICI MEHMET,Genç Engin,Çelikgün Serkan,BAŞ EKİCİ Hacer The Effect of Age and Gender on Some Hematological Parameters and Erythrocyte Osmotic Fragility in Kangal Shepherd Dogs. Atatürk Üniversitesi Veteriner Bilimleri Dergisi 16, no.3 (2021): 298 - 305. 10.17094/ataunivbd.1051477
MLA	KOÇKAYA Mustafa,EKICI MEHMET,Genç Engin,Çelikgün Serkan,BAŞ EKİCİ Hacer The Effect of Age and Gender on Some Hematological Parameters and Erythrocyte Osmotic Fragility in Kangal Shepherd Dogs. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, vol.16, no.3, 2021, ss.298 - 305. 10.17094/ataunivbd.1051477
AMA	KOÇKAYA M,EKICI M,Genç E,Çelikgün S,BAŞ EKİCİ H The Effect of Age and Gender on Some Hematological Parameters and Erythrocyte Osmotic Fragility in Kangal Shepherd Dogs. Atatürk Üniversitesi Veteriner Bilimleri Dergisi. 2021; 16(3): 298 - 305. 10.17094/ataunivbd.1051477
Vancouver	KOÇKAYA M,EKICI M,Genç E,Çelikgün S,BAŞ EKİCİ H The Effect of Age and Gender on Some Hematological Parameters and Erythrocyte Osmotic Fragility in Kangal Shepherd Dogs. Atatürk Üniversitesi Veteriner Bilimleri Dergisi. 2021; 16(3): 298 - 305. 10.17094/ataunivbd.1051477
IEEE	KOÇKAYA M,EKICI M,Genç E,Çelikgün S,BAŞ EKİCİ H "The Effect of Age and Gender on Some Hematological Parameters and Erythrocyte Osmotic Fragility in Kangal Shepherd Dogs." Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 16, ss.298 - 305, 2021. 10.17094/ataunivbd.1051477
ISNAD	KOÇKAYA, Mustafa vd. "The Effect of Age and Gender on Some Hematological Parameters and Erythrocyte Osmotic Fragility in Kangal Shepherd Dogs". Atatürk Üniversitesi Veteriner Bilimleri Dergisi 16/3 (2021), 298-305. https://doi.org/10.17094/ataunivbd.1051477