Effect of Imidocarb on DNA Damage in Sheep with Babesiosis
Yıl: 2022 Cilt: 28 Sayı: 1 Sayfa Aralığı: 115 - 120 Metin Dili: İngilizce DOI: 10.9775/kvfd.2021.2660 İndeks Tarihi: 13-06-2022
Effect of Imidocarb on DNA Damage in Sheep with Babesiosis
Öz: n this study, it was aimed to determine the DNA damage using the comet assay, which specifically shows DNA damage in naturally
Babesia spp.-infected sheep and to evaluate the damage before and after imidocarb application. Blood samples obtained from 10
infected sheep with positive clinical signs and symptoms of babesiosis and whose diagnosis was confirmed by Giemsa staining and PCR
methods, and blood samples from 10 healthy sheep were used as study material. DNA damage was examined by the comet assay from
the blood samples of the infected patient group and the control group obtained during the disease and after the treatment, and the
results were compared with statistical methods. When DNA damage was examined in sick animals diagnosed with babesiosis, the tail
length and the tail moment values were found to be statistically significantly higher than the control group (P<0.001). According to the
results obtained after imidocarb application, it was determined that DNA damage and tail moment decreased statistically with imidocarb,
and the difference was statistically significant, and the values were higher than the control group (P<0.001). As a result, Babesia infection
can cause DNA damage, has been confirmed by the determination of direct DNA damage using the comet assay, and imidocarb given
for treatment was successful and reduced the damage.
Anahtar Kelime: Babeziozisli Koyunlarda İmidokarb Uygulamasının DNA Hasarına Etkisi
Öz: hasarının belirlenmesi ve imidokarb uygulaması öncesi ve sonrası hasarın değerlendirilmesi amaçlanmıştır. Çalışma materyali
olarak babeziozis klinik belirti ve semptomları pozitif olan ve Giemsa boyama ve PCR yöntemleri ile tanısı doğrulanan 10 enfekte
koyundan alınan kan örnekleri ve 10 sağlıklı koyundan alınan kan örnekleri kullanıldı. Enfekte hasta grubu ve kontrol grubundan
hastalık sırasında ve tedavi sonrasında alınan kan örneklerinden comet testi ile DNA hasarı incelendi ve sonuçlar istatistiksel
yöntemlerle karşılaştırıldı. Babeziozis tanısı konulan hasta hayvanlarda DNA hasarı incelendiğinde kuyruk uzunluğu ve kuyruk
momenti değerleri kontrol grubuna göre istatistiksel olarak anlamlı derecede yüksek bulundu (P<0.001). İmidokarb uygulaması
sonrası elde edilen sonuçlara göre DNA hasarı ve kuyruk momentinin imidokarb ile istatistiksel olarak azaldığı ve aradaki farkın
istatistiksel olarak anlamlı olduğu ve değerlerin kontrol grubuna göre daha yüksek olduğu belirlendi (P<0.001). Sonuç olarak Babesia
enfeksiyonunun DNA hasarına neden olabileceği, comet testi kullanılarak direkt DNA hasarının belirlenmesi ile doğrulanmış ve
tedavi için verilen imidocarb başarılı olmuş ve hasarı azaltmıştır
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | ONER A, ayan a, orunç kılınç ö, USTA A, ertaş oğuz f (2022). Effect of Imidocarb on DNA Damage in Sheep with Babesiosis. , 115 - 120. 10.9775/kvfd.2021.2660 |
| Chicago | ONER AHMET CIHAT,ayan adnan,orunç kılınç özlem,USTA Ayşe,ertaş oğuz fatma Effect of Imidocarb on DNA Damage in Sheep with Babesiosis. (2022): 115 - 120. 10.9775/kvfd.2021.2660 |
| MLA | ONER AHMET CIHAT,ayan adnan,orunç kılınç özlem,USTA Ayşe,ertaş oğuz fatma Effect of Imidocarb on DNA Damage in Sheep with Babesiosis. , 2022, ss.115 - 120. 10.9775/kvfd.2021.2660 |
| AMA | ONER A,ayan a,orunç kılınç ö,USTA A,ertaş oğuz f Effect of Imidocarb on DNA Damage in Sheep with Babesiosis. . 2022; 115 - 120. 10.9775/kvfd.2021.2660 |
| Vancouver | ONER A,ayan a,orunç kılınç ö,USTA A,ertaş oğuz f Effect of Imidocarb on DNA Damage in Sheep with Babesiosis. . 2022; 115 - 120. 10.9775/kvfd.2021.2660 |
| IEEE | ONER A,ayan a,orunç kılınç ö,USTA A,ertaş oğuz f "Effect of Imidocarb on DNA Damage in Sheep with Babesiosis." , ss.115 - 120, 2022. 10.9775/kvfd.2021.2660 |
| ISNAD | ONER, AHMET CIHAT vd. "Effect of Imidocarb on DNA Damage in Sheep with Babesiosis". (2022), 115-120. https://doi.org/10.9775/kvfd.2021.2660 |
| APA | ONER A, ayan a, orunç kılınç ö, USTA A, ertaş oğuz f (2022). Effect of Imidocarb on DNA Damage in Sheep with Babesiosis. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 28(1), 115 - 120. 10.9775/kvfd.2021.2660 |
| Chicago | ONER AHMET CIHAT,ayan adnan,orunç kılınç özlem,USTA Ayşe,ertaş oğuz fatma Effect of Imidocarb on DNA Damage in Sheep with Babesiosis. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 28, no.1 (2022): 115 - 120. 10.9775/kvfd.2021.2660 |
| MLA | ONER AHMET CIHAT,ayan adnan,orunç kılınç özlem,USTA Ayşe,ertaş oğuz fatma Effect of Imidocarb on DNA Damage in Sheep with Babesiosis. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.28, no.1, 2022, ss.115 - 120. 10.9775/kvfd.2021.2660 |
| AMA | ONER A,ayan a,orunç kılınç ö,USTA A,ertaş oğuz f Effect of Imidocarb on DNA Damage in Sheep with Babesiosis. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2022; 28(1): 115 - 120. 10.9775/kvfd.2021.2660 |
| Vancouver | ONER A,ayan a,orunç kılınç ö,USTA A,ertaş oğuz f Effect of Imidocarb on DNA Damage in Sheep with Babesiosis. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2022; 28(1): 115 - 120. 10.9775/kvfd.2021.2660 |
| IEEE | ONER A,ayan a,orunç kılınç ö,USTA A,ertaş oğuz f "Effect of Imidocarb on DNA Damage in Sheep with Babesiosis." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 28, ss.115 - 120, 2022. 10.9775/kvfd.2021.2660 |
| ISNAD | ONER, AHMET CIHAT vd. "Effect of Imidocarb on DNA Damage in Sheep with Babesiosis". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 28/1 (2022), 115-120. https://doi.org/10.9775/kvfd.2021.2660 |
