Aim: Studies conducted on young adults in the context of risky behaviors and habits show that the importance and prevalence of substance addiction is gradually increasing. This study was conducted to investigate the prevalence of cigarette, alcohol and drug use among university students and related factors. Method: The population of this cross-sectional study consisted of 2536 students studying at the associate, undergraduate and graduate levels at Tarsus University. The questionnaire forms were delivered to all of the students by online method and 536 responses were obtained. Sample selection was not made, 22% of the population was reached. In the study, descriptive statistics such as number, ratio, mean, and standard deviation and the relative risks were calculated from the four-cell tables. Results: 26.9% of the participants stated that they used cigarettes, 17.6% alcohol, and 2.4% drugs. The risk of using a substance at least once in a lifetime increases 12.4 times with the presence of a substance user in the immediate environment, 9.5 times by being an active cigarette smoker, and 8.9 times by repeating a grade. Conclusion: The importance of awareness-raising programs for young people and families regarding addictive substance use has also been emerged in this study. In particular, it is considered important to prioritize efforts to prevent smoking, which is first tried and increases the risk factor for using other substances.