Aim: Helicobacter pylori is a bacterium that is located in human gastric mucosa and causes chronic gastritis and peptic ulcer disease and it is determined to be a risk factor in MALT lymphoma and gastric adenocarcinoma. İn this study, we aimed to retrospectively investigate the frequency of H. pylori antigen in the stool of patients coming with dyspepsia and their distribution according to gender and clinics. Material and Methods: The presence of H. pylori antigen was evaluated retrospectively in feces sent from polyclinics and services of Ondokuz Mayıs University Medical Faculty Hospital between 2016-2019. The Certest (Biotec, Spain) kit was used for antigen detection using monoclonal antibodies based on immunochromotography, the manufacturer indicate its sensitivity at 98.2% whereas specificity at 98.4%. Chi-square test was used in the statistical analysis of the data. significance level was accepted as p<0.05. Results: H. pylori antigen was found positive in 569 (16.9%) of 3349 stool samples included in the study. When we look at the positivity rates by years, the most positivity was found in 2018 (21.8%) and it was found to be statistically significant (p<0.001). When compared with age groups, a significant difference was found in positivity rates (p<0.001). The highest positivity was observed between the ages of 40-49 (23%). Conclusion: Although the frequency of H.pylori in our study was lower than in previous years studies, it is still an important problem in terms of public health. We believe that early diagnosis and treatment of the pathogen should be provided with cost-effective, easily applicable test strategies.