Investigation of the effıciency of pomegranate (punica granatum l.) Peel extract on herpes simplex virus-1
Yıl: 2022 Cilt: 38 Sayı: 2 Sayfa Aralığı: 59 - 65 Metin Dili: İngilizce DOI: 10.15312/EurasianJVetSci.2022.365 İndeks Tarihi: 20-06-2022
Investigation of the effıciency of pomegranate (punica granatum l.) Peel extract on herpes simplex virus-1
Öz: Amaç: Bu çalışmada nar kabuğu (Punica granatum L.) ekstraktının in vitro
olarak HSV-1 üzerine antiviral etkinliğinin belirlenmesi amaçlanmıştır.
Gereç ve Yöntem: Antiviral etkinlik değerlendirilmeden önce, nar kabuğu
ekstratının saf su ile dilue edilmiş farklı konsantrasyonlarının Vero hücre kültüründe sitotoksik aktivitesi belirlendi. Bu hücre kültüründe 0,87 mg/mL konsantrasyondan daha düşük konsantrasyonlarda sitotoksiteye neden olmadığı
tespit edildi. Antiviral etkinlik MTT testi ve mikroskobik analizler sonrasında
sitopatojenik etkinin (CPE) varlığıyla belirlendi.
Bulgular: Nar kabuğu ekstratının 0,87 mg/mL ila 0,87x2-5mg/mL arasındaki
konsantrasyonlarda HSV-1’e karşı önemli bir antiviral etkinliği olduğu belirlendi. Bu etkinin virusun hücreye girişine engel olmasıyla sağlamış olabilir.
Öneri: Nar kabuğu ekstraktının HSV-1’e karşı in vitro olarak antiviral etkinliğe sahip olduğu ve elde edilen sonuçlar doğrultusunda Punica granatum
L. ekstratının antiviral etkinliğinin daha detaylı belirlenebilmesi için in vivo
denemelere ihtiyaç olduğu düşünüldü. Ayrıca Punica granatum L. Ekstratının
Herpes viruslar ve diğer viruslara karşı etkinliğinin detaylı bir şekilde belirlenmesi gelecekte antiviral ilaç denemelerine katkı sağlayacaktır.
Anahtar Kelime: Nar (punica granatum l.) kabuğu ekstratının herpes simplex virus-1 üzerine etkinliğinin araştırılması
Öz: Aim: This study, it was aimed to determine the antiviral activity of pomegranate peel (Punica granatum L.) extract on HSV-1 in vitro.
Materials and Methods: Before evaluating the antiviral efficacy, the cytotoxic
activity of different concentrations of pomegranate peel extract diluted with
distilled water in Vero cell culture was determined. It was determined that this
cell culture did not cause cytotoxicity at concentrations lower than 0.87 mg/
mL. Antiviral efficacy was determined by cytopathogenic effect (CPE) after the
MTT test and microscopic analysis.
Results: It was determined that pomegranate peel extract had significant
antiviral activity against HSV-1 at concentrations between 0.87 mg/mL and
0.87x2-5mg/mL. This effect may have been achieved by preventing the virus
from entering the cell.
Conclusion: It was thought that pomegranate peel extract has antiviral activity against HSV-1 in vitro, and in line with the results obtained, in vivo trials
are needed to determine the antiviral activity of Punica granatum L. extract in
more detail. In addition, a precise determination of the effectiveness of Punica
granatum L. Extract against Herpes viruses and other viruses will contribute
to antiviral drug trials in the future.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | DİK I, ASLIM H (2022). Investigation of the effıciency of pomegranate (punica granatum l.) Peel extract on herpes simplex virus-1. , 59 - 65. 10.15312/EurasianJVetSci.2022.365 |
| Chicago | DİK IRMAK,ASLIM Hatice Pelin Investigation of the effıciency of pomegranate (punica granatum l.) Peel extract on herpes simplex virus-1. (2022): 59 - 65. 10.15312/EurasianJVetSci.2022.365 |
| MLA | DİK IRMAK,ASLIM Hatice Pelin Investigation of the effıciency of pomegranate (punica granatum l.) Peel extract on herpes simplex virus-1. , 2022, ss.59 - 65. 10.15312/EurasianJVetSci.2022.365 |
| AMA | DİK I,ASLIM H Investigation of the effıciency of pomegranate (punica granatum l.) Peel extract on herpes simplex virus-1. . 2022; 59 - 65. 10.15312/EurasianJVetSci.2022.365 |
| Vancouver | DİK I,ASLIM H Investigation of the effıciency of pomegranate (punica granatum l.) Peel extract on herpes simplex virus-1. . 2022; 59 - 65. 10.15312/EurasianJVetSci.2022.365 |
| IEEE | DİK I,ASLIM H "Investigation of the effıciency of pomegranate (punica granatum l.) Peel extract on herpes simplex virus-1." , ss.59 - 65, 2022. 10.15312/EurasianJVetSci.2022.365 |
| ISNAD | DİK, IRMAK - ASLIM, Hatice Pelin. "Investigation of the effıciency of pomegranate (punica granatum l.) Peel extract on herpes simplex virus-1". (2022), 59-65. https://doi.org/10.15312/EurasianJVetSci.2022.365 |
| APA | DİK I, ASLIM H (2022). Investigation of the effıciency of pomegranate (punica granatum l.) Peel extract on herpes simplex virus-1. Eurasian Journal of Veterinary Sciences, 38(2), 59 - 65. 10.15312/EurasianJVetSci.2022.365 |
| Chicago | DİK IRMAK,ASLIM Hatice Pelin Investigation of the effıciency of pomegranate (punica granatum l.) Peel extract on herpes simplex virus-1. Eurasian Journal of Veterinary Sciences 38, no.2 (2022): 59 - 65. 10.15312/EurasianJVetSci.2022.365 |
| MLA | DİK IRMAK,ASLIM Hatice Pelin Investigation of the effıciency of pomegranate (punica granatum l.) Peel extract on herpes simplex virus-1. Eurasian Journal of Veterinary Sciences, vol.38, no.2, 2022, ss.59 - 65. 10.15312/EurasianJVetSci.2022.365 |
| AMA | DİK I,ASLIM H Investigation of the effıciency of pomegranate (punica granatum l.) Peel extract on herpes simplex virus-1. Eurasian Journal of Veterinary Sciences. 2022; 38(2): 59 - 65. 10.15312/EurasianJVetSci.2022.365 |
| Vancouver | DİK I,ASLIM H Investigation of the effıciency of pomegranate (punica granatum l.) Peel extract on herpes simplex virus-1. Eurasian Journal of Veterinary Sciences. 2022; 38(2): 59 - 65. 10.15312/EurasianJVetSci.2022.365 |
| IEEE | DİK I,ASLIM H "Investigation of the effıciency of pomegranate (punica granatum l.) Peel extract on herpes simplex virus-1." Eurasian Journal of Veterinary Sciences, 38, ss.59 - 65, 2022. 10.15312/EurasianJVetSci.2022.365 |
| ISNAD | DİK, IRMAK - ASLIM, Hatice Pelin. "Investigation of the effıciency of pomegranate (punica granatum l.) Peel extract on herpes simplex virus-1". Eurasian Journal of Veterinary Sciences 38/2 (2022), 59-65. https://doi.org/10.15312/EurasianJVetSci.2022.365 |
