Detection and molecular characterization of mastadenoviruses in calves with respiratory system infection
Yıl: 2022 Cilt: 38 Sayı: 2 Sayfa Aralığı: 83 - 89 Metin Dili: İngilizce DOI: 10.15312/EurasianJVetSci.2022.368 İndeks Tarihi: 20-06-2022
Detection and molecular characterization of mastadenoviruses in calves with respiratory system infection
Öz: Aim: Bovine adenovirus (BAdV) is one of the viral agents that causes respiratory illness in cattle, along with numerous other viral agents. Although the prevalence of BAdV has been determined by serological studies conducted in our
country, there are limited data on the detection of BAdVs in respiratory system
samples, and there is no study on their molecular characterization. The aim of
this study was to detect and characterize BAdVs of the Mastadenovirus genus.
Materials and Methods: In this study, a total of 64 nasal swabs and one
lung sample from 65 cattle of different ages with respiratory system disease
symptoms were used. After the extraction of viral DNA, they were tested by
PCR using primers targeting the hexon gene region and the expected size of
amplicons were sequenced.
Results: According to the PCR results, three samples were found positive and
the positivity rate was detected as 4.6%. Out of positive samples, two were
able to be sequenced and one clustered in the BAdV-2 serotype while the other
was grouped in the BAdV-3 serotype.
Conclusion: In this study, it was revealed that BAdVs are contributing agents
in respiratory system infection in our country. Also, this is the first study reporting the molecular characterization of BAdV-2 and BAdV-3 in calves with
respiratory system infection in Turkey.
Anahtar Kelime: Solunum sistemi enfeksiyonu olan buzağılarda mastadenovirusların tespiti ve moleküler karakterizasyonu
Öz: Amaç: Bovine adenoviruslar (BAdV), birçok viral etkenle birlikte sığırlarda solunum sistemi hastalığına neden olan patojenlerden biri olarak kabul edilmektedir. Ülkemizde BAdV prevalansı, yapılan serolojik çalışmalarla tespit edilmiş
olmasına rağmen, solunum sistemi örneklerinde BAdV'nin saptanmasına
ilişkin veriler sınırlıdır ve moleküler karakterizasyonları ile ilgili herhangi bir
çalışma bulunmamaktadır. Bu çalışmada Mastadenovirus genusunda bulunan
BAdV’ların tespiti ve moleküler karakterizasyonu amaçlanmıştır.
Gereç ve Yöntem: Bu çalışmada, solunum sistemi hastalığı semptomları olan
farklı yaştaki 65 sığırdan alınan toplam 64 burun sürüntüsü ve bir akciğer örneği kullanıldı. Viral DNA'nın ekstraksiyonunu takiben, örnekler hekzon gen
bölgesini hedefleyen primerler kullanılarak PCR ile test edildi ve beklenen büyüklükteki amplikonların dizin bilgisi elde edildi.
Bulgular: PCR sonucunda üç örnek pozitif bulunmuş ve pozitiflik oranı %4,6
olarak tespit edilmiştir. Dizin bilgileri elde edilebilen iki örnekten birinin
BAdV-2 serotipinde, diğerinin ise BAdV-3 serotipinde yer aldığı saptanmıştır.
Öneri: Bu çalışmada BAdV'lerin ülkemizde solunum sistemi enfeksiyonuna
katkıda bulunan ajanlar olduğu ortaya koyulmuştur. Ayrıca bu, Türkiye'de solunum sistemi enfeksiyonu olan buzağılarda BAdV-2 ve BAdV-3'ün moleküler
karakterizasyonunu bildiren ilk çalışmadır
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Karayel-Hacioglu I, Alkan F, Duran-Yelken S (2022). Detection and molecular characterization of mastadenoviruses in calves with respiratory system infection. , 83 - 89. 10.15312/EurasianJVetSci.2022.368 |
| Chicago | Karayel-Hacioglu Ilke,Alkan Feray,Duran-Yelken Selda Detection and molecular characterization of mastadenoviruses in calves with respiratory system infection. (2022): 83 - 89. 10.15312/EurasianJVetSci.2022.368 |
| MLA | Karayel-Hacioglu Ilke,Alkan Feray,Duran-Yelken Selda Detection and molecular characterization of mastadenoviruses in calves with respiratory system infection. , 2022, ss.83 - 89. 10.15312/EurasianJVetSci.2022.368 |
| AMA | Karayel-Hacioglu I,Alkan F,Duran-Yelken S Detection and molecular characterization of mastadenoviruses in calves with respiratory system infection. . 2022; 83 - 89. 10.15312/EurasianJVetSci.2022.368 |
| Vancouver | Karayel-Hacioglu I,Alkan F,Duran-Yelken S Detection and molecular characterization of mastadenoviruses in calves with respiratory system infection. . 2022; 83 - 89. 10.15312/EurasianJVetSci.2022.368 |
| IEEE | Karayel-Hacioglu I,Alkan F,Duran-Yelken S "Detection and molecular characterization of mastadenoviruses in calves with respiratory system infection." , ss.83 - 89, 2022. 10.15312/EurasianJVetSci.2022.368 |
| ISNAD | Karayel-Hacioglu, Ilke vd. "Detection and molecular characterization of mastadenoviruses in calves with respiratory system infection". (2022), 83-89. https://doi.org/10.15312/EurasianJVetSci.2022.368 |
| APA | Karayel-Hacioglu I, Alkan F, Duran-Yelken S (2022). Detection and molecular characterization of mastadenoviruses in calves with respiratory system infection. Eurasian Journal of Veterinary Sciences, 38(2), 83 - 89. 10.15312/EurasianJVetSci.2022.368 |
| Chicago | Karayel-Hacioglu Ilke,Alkan Feray,Duran-Yelken Selda Detection and molecular characterization of mastadenoviruses in calves with respiratory system infection. Eurasian Journal of Veterinary Sciences 38, no.2 (2022): 83 - 89. 10.15312/EurasianJVetSci.2022.368 |
| MLA | Karayel-Hacioglu Ilke,Alkan Feray,Duran-Yelken Selda Detection and molecular characterization of mastadenoviruses in calves with respiratory system infection. Eurasian Journal of Veterinary Sciences, vol.38, no.2, 2022, ss.83 - 89. 10.15312/EurasianJVetSci.2022.368 |
| AMA | Karayel-Hacioglu I,Alkan F,Duran-Yelken S Detection and molecular characterization of mastadenoviruses in calves with respiratory system infection. Eurasian Journal of Veterinary Sciences. 2022; 38(2): 83 - 89. 10.15312/EurasianJVetSci.2022.368 |
| Vancouver | Karayel-Hacioglu I,Alkan F,Duran-Yelken S Detection and molecular characterization of mastadenoviruses in calves with respiratory system infection. Eurasian Journal of Veterinary Sciences. 2022; 38(2): 83 - 89. 10.15312/EurasianJVetSci.2022.368 |
| IEEE | Karayel-Hacioglu I,Alkan F,Duran-Yelken S "Detection and molecular characterization of mastadenoviruses in calves with respiratory system infection." Eurasian Journal of Veterinary Sciences, 38, ss.83 - 89, 2022. 10.15312/EurasianJVetSci.2022.368 |
| ISNAD | Karayel-Hacioglu, Ilke vd. "Detection and molecular characterization of mastadenoviruses in calves with respiratory system infection". Eurasian Journal of Veterinary Sciences 38/2 (2022), 83-89. https://doi.org/10.15312/EurasianJVetSci.2022.368 |
