Yumurta sarısına enjekte edilen bisfenol A' nın tavuklarda iskelet kası gelişimi üzerindeki etkileri
Yıl: 2022 Cilt: 38 Sayı: 2 Sayfa Aralığı: 90 - 100 Metin Dili: Türkçe DOI: 10.15312/EurasianJVetSci.2022.369 İndeks Tarihi: 20-06-2022
Yumurta sarısına enjekte edilen bisfenol A' nın tavuklarda iskelet kası gelişimi üzerindeki etkileri
Öz: Amaç: Bu çalışmada, yumurtaya verilen farklı dozlarda BPA’nın iskelet
kası gelişimi üzerindeki etkilerinin histolojik metotlarla belirlenmesi
amaçlanmaktadır.
Gereç ve Yöntem: Isa Brown ırkı sağlıklı ticari anaçlardan elde edilen
310 adet döllü tavuk yumurtası kontrol, taşıyıcı, 50,100 ve 250 μg/yumurta olmak üzere 5 gruba ayrıldı. Test solüsyonları inkübasyon öncesi
yumurta sarısına enjekte edildi. Kuluçkanın 13, 18 ve 21. günlerinde, her
gruptan 10 canlı embriyo elde edilinceye kadar rastgele yumurta seçilerek açıldı. Embriyolardan alınan iskelet kası (musculus fibularis longus,
musculus sternocoracoideus pectoralis) doku örnekleri %10'luk formol
solüsyonunda tespit edildi ve rutin histolojik tekniklerle işlenerek parafinde bloklandı. Kesitler Crossmon’un üçlü boyama yöntemi ile boyandı.
PCNA (Proliferating cell nuclear antigen) reaktivitesi ise immünohistokimyasal yöntemle belirlendi.
Bulgular: Kas gelişiminin BPA uygulanan gruplarda geri kaldığı ve PCNA
pozitif hücre sayısının da daha yüksek olduğu dikkati çekti (p<0.05). Söz
konusu bu gruplarda enine bandlaşmanın çok zayıf olduğu ve miyotüp organizasyonunun ise henüz tamamlanmadığı gözlendi.
Öneri: BPA'nın iskelet kası dokusunun gelişimi üzerindeki olumsuz etkilerinin ileride hayvanlardan beklenen bazı performans parametrelerinde
düşüşe neden olabileceği sonucuna varılmıştır.
Anahtar Kelime: The effects of bisphenol A injected into egg yolk on skeletal muscle development in chicken
Öz: Aim: The aim of this study was to determine of the effects of in ovo administered BPA on embryonic development skeletal muscle using histological
methods in chickens.
Materials and Methods: 310 fertile eggs of Isa Brown laying parent stock
were divided into 5 groups as control, vehicle control, 50, 100, and 250
μg/egg BPA. At the 13th, 18th and 21st days of incubation, eggs were
randomly opened from each group until 10 live embryos were obtained.
Skeletal muscle tissue samples (musculus fibularis longus, musculus sternocoracoideus pectoralis) taken from embryos were fixed in 10% formol
solution and processed with routine histological techniques and immersed
in paraffin. Sections were stained with Crossmon's triple staining method.
Also, PCNA (Proliferating cell nuclear antigen) reactivity was determined
by immunohistochemical method.
Results: Embryonic development of skeletal muscle was retarded in BPA
treated groups. The mean count of PCNA positive cells significantly increased in BPA treated groups when compared to control and vehicle control
groups on 21 day of incubation (p<0.05).
Conclusion: These results have suggested that developmental exposure to
BPA adversely affected development of skeletal muscle.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- Acconcia F, Pallottini V, Marino M, (2015). Molecular mechanisms of action of BPA. Dose-Response: An International Journal, 13(4), 1–9.
- Ballesteros-Gómez A, Rubio S, Pérez-Bendito D, 2009. Analytical methods for the determination of bisphenol A in food. J Chromatogr A, 1216(3), 449-69.
- Banlunara W, Bintvihok A, Kumagai S, 2005. Immunohistochemical study of proliferating cell nuclear antigen (PCNA) in duckling liver fed with aflatoxin B1 and esterified glucomannan. Toxicon, 46(8), 954-7.
- Bauer R, Plieschnig JA, Finkes T, Riegler B, et al., 2013. The developing chicken yolk sac acquires nutrient transport competence by an orchestrated differentiation process of its endodermal epithelial cells. J Biol Chem, 288(2), 1088- 98.
- Berg C, 2000. Environmental pollutants and the reproductive system in birds. PHD Thesis, Acta Universitatis Upsaliensis Faculty of Science and Technology, Uppsala.
- Berg C, Blomqvist A, Holm L, Brandt I, et al., 2004. Embryonic exposure to oestrogen causes eggshell thinning and altered shell gland carbonic anhydrase expression in the domestic hen. Reproduction, 128(4), 455-61.
- Berg C, Halldin K, Brunström B, 2001. Effects of bisphenol A and tetrabromobisphenol A on sex organ development in quail and chicken embryos. Environ Toxicol Chem, 20(12), 2836-40.
- Berg C, Halldin K, Fridolfsson AK, Brandt I, et al., 1999. The avian egg as a test system for endocrine disrupters: effects of diethylstilbestrol and ethynylestradiol on sex organ develop. Sci Total Environ, 233(1-3), 57-66.
- Chapin RE, Adams J, Boekelheide K, Leg J, et al., 2008. NTPCERHR expert panel report on the reproductive and developmental toxicity of bisphenol A. Birth Defects Res B Dev Reprod Toxicol, 83(3), 157–395.
- Clayton EMR, Todd M, Dowd JB, Aiello AE 2011. The impact of bisphenol A and triclosan on immune parameters in the U.S. population, NHANES 2003–2006. Environ Health Perspect, 119(3), 390-6.
- Cooper JE, Kendig EL, Belcher SM, 2011. Assessment of bisphenol A released from reusable plastic, aluminium and stainless steel water bottles. Chemosphere, 85(6), 943-7.
- Crain DA, Eriksen M, Iguchi T, Jobling S, et al., 2007. An ecological assessment of bisphenol-A: evidence from comparative biology. Reprod Toxicol, 24(2), 225-39.
- Dessi-Fulgheri F, Porrini S, Farabollini F, 2002. Effects of perinatal exposure to bisphenol A on play behavior of female and male juvenile rats. Environ Health Perspect, 110(3), 403–407.
- Drake VJ, Koprowski SL, Lough JW, Smith SM, 2006. Gastrulating chick embryo as a model for evaluating teratogenicity: a comparison of three approaches birth defects. Res A Clin Mol Teratol, 76(1), 66-71.
- Fernandez MF, Arrebola JP, Taoufiki J, Navalón A, et al., 2007. Bisphenol-A and chlorinated derivatives in adipose tissue of women. Reprod Toxicol, 24(2), 259–64.
- Flint S, Markle T, Thompson S, Wallace E, 2012. Bisphenol A exposure, effects, and policy: a wildlife perspective. J Environ Manage, 104, 19-34. Geens T, Aerts D, Berthot C, Bourguignon J, et al., 2012. A review of dietary and non-dietary exposure to bisphenol-A.
- Food Chem Toxicol, 50 (10), 3725–40. Goa GY, Leea SJ, Joa A, Leeb JR, 2018. Bisphenol A and estradiol impede myoblast differentiation through downregulating Akt signaling pathway. Toxicol Lett, 292, 12–19.
- EFSA (European Food Safety Authority) Gundert-Remy U, Bodin J, Bosetti C, FitzGerald RE, et al., 2017. Bisphenol A (BPA) hazard assessment protocol, technical report. EFSA
- Supporting Publication 2017: EN-1354. 76 pp. https://doi. org/10.2903/sp.efsa.2017.EN-1354.
- Gündüz N, Öznurlu Y, 2014. Adverse effects of aflatoxin B1 on skeletal muscle development in broiler chickens. Br Poult Sci, 55 (5), 684–92.
- Halldinn K, Berg C, Bergman A, 2001. Distrubution of Bisphenol A and tetrabromobisphenol A in quail eggs, embryos and laying birds and studies on reproduction variables in adults following in ovo exposure. Arch Toxicol, 75(10), 597-603.
- Henry MH and Burke WH, 1998. Sexual dimorphism in broiler chick embryos and embryonic muscle development in late ıncubation. Poultry Science , 77, 728-736.
- Ikezuki Y, Tsutsumi O, Takai Y, Kamei Y, et al., 2002. Determination of bisphenol A concentrations in human biological fluids reveals significant early prenatal exposure. Hum Reprod, 17(11), 2839-41.
- Jelinek R, 1977. Methods in prenatal toxicology. In: The chick embryotoxicity screening test (CHEST). Ed: Neubert D, Merker H, Kwasigrooh T, Georg Thieme, Stutgort, p. 381-6.
- Jessl L, Rebecca Lenz R, Massing FG, Scheider J, et al., 2018. Effects of estrogens and antiestrogens on gonadal sex differentiation and embryonic development in the domestic fowl (Gallus gallus domesticus). PeerJ, 6, e5094.
- Kandil B, Sur E, 2018. The light microscopic investigation of the effects of in-ovo administered bisphenol A (BPA) on the development of testes. Ankara Univ Vet Fak Derg, 65(3), 273-82.
- Kemper F, Luepke N, 1986. Toxicity testing by the hen's egg test (HET). Food Chem Toxicol, 24(6-7), 647-8.
- Kikuchi T, 1971. Studies on development and differentiation of muscle III. Especially on the mode of increase in the number of muscle cells. Tohoku J Agric Res, 22, 1–15.
- Latshaw WK, 1987. Veterinary Developmental Anatomy. Toronto, Philadelphia, p.184-204. Li DK, Zhou Z, Miao M, He Y, et al., 2011. Urine bisphenol-A (BPA) level in relation to semen quality. Fertil Steril, 95(2), 625–30.
- Liao C, Kannan K, 2011. Widespread occurrence of bisphenol A in paper and paper products: implications for human exposure. Environ Sci Technol, 45 (21),9372-9.
- Liu Y, Mei C, Liu H, Wang H, et al., 2014. Modulation of cytokine expression in human macrophages by endocrine-disrupting chemical Bisphenol-A. Biochem Biophys Res Commun, 451(4), 592-8.
- Manfo FPT, Jubendradass R, Nantia EA, Moundipa PF, et al., 2014. Adverse effects of bisphenol A on male reproductive function. Rev Environ Contam Toxicol, 228, 57-82.
- Meltzer M, Pfeiffer E, 2001. Chemistry of natural and anthropogenic endocrine active compouds: The handbook of enviromental chemistry, endocrine disruptors Part 1, Meltzer M(ed), Springer- Verlag Berlin Heidelberg, p. 63-80.
- Miyawaki J, Sakayama K, Kato H, Yamamoto H, et al., 2007. Perinatal and postnatal exposure to bisphenol a increases adipose tissue mass and serum cholesterol level in mice. J Atheroscler Thromb, 14, 245-252.
- Moore DT, Ferket PR, Mozdziak PE, 2005. Muscle Development in the late embryonic and early post-hatch poult. Int J Poult Sci, 4(3), 138-142.
- Nakamura K, Itoh K, Sugimoto T, Fushiki S, 2007. Prenatal exposure to bisphenol A affects adult murine neocortical structure. Neurosci¬ Lett, 420(2), 100-5.
- Nakamura K, Itoh K, Yaoi T, Fujiwara Y, et al., 2006. Murine neocortical histogenesis is perturbed by prenatal exposure to low doses of Bisphenol A. J Neurosci Res, 84(6), 1197- 1205.
- Nakamura K, Kariyazono H, 2010. Influence of endocrinedisrupting chemicals on the immune system. J Health Sci, 56, 361–73.
- Ouyang H, Wang Z, Chen X, Yu J, et al., 2017. Proteomic Analysis of Chicken Skeletal Muscle during Embryonic Development.FrontPhysiol,8,281.doi:10.3389/fphys.2017.00281
- Özaydın T, Öznurlu Y, Sur E, Celik İ, et al., 2018b. Effects of bisphenol A on antioxidant system and lipid profile in rats. Biotech Histochem, 93(4), 231-238.
- Özaydın T, Öznurlu Y, Sur E, Çelik İ, et al., 2018a. The effects of bisphenol A on some plasma cytokine levels and distribution of CD8+ and CD4+ T lymphocytes in spleen, ileal Peyer’s patch and bronchus associated lymphoid tissue in rats. Acta Histochem, 120(8), 728–733.
- Öznurlu Y, Celik I, Sur E, Özaydın T, et al., 2012. Determination of the effects of aflatoxin B1 given in ovo on the proximal tibial growth plate of broiler chickens: histological, histometric and immunohistochemical findings. Avian Pathol, 41(5) 469–77.
- Pisapia L, Del Pozzo G, Barba P, Caputo L, et al., 2012. Effects of some endocrine disruptors on cell cycle progression and murine dendritic cell differentiation. Gen Comp Endocrinol, 178(1), 54-63.
- Ramos JG, Varayoud J, Kass L, Rodríguez H, Costabel L, Muñoz-De-Toro M, Luque EH, 2003. Bisphenol A induces both transient and permanent histofunctional alterations of the hypothalamic-pituitary-gonadal axis in prenatally exposed male rats. Endocrinology, 144, 3206–3215.
- Receno C, Benson M, Liang C, Keslacy S, et al., 2014. Effect of bisphenol A (BPA) on skeletal muscle oxidative stress. Int J Exerc Sci, Conference Proceedings, Vol 9, Iss 2, Article 66.
- Remignon H, Gardahaut MF, Marche G, Ricard FH, 1995. Selection for rapid growth increases the number and the size of muscle fibres without changing their typing in chickens. J Muscle Res Cell Motil, 16(2),95-102.
- Richter CA, Birnbaum LS, Farabollini F, Newbold RR, et al., 2007. In vivo effects of bisphenol A in laboratory rodent studies. Reprod Toxicol, 24(2), 199-224.
- Romano ME, Webster GM, Vuong AM, Thomas Zoeller R, et al., 2015. Gestational urinary bisphenol A and maternal and newborn thyroid hormone concen¬trations: the Home Study. Environ Res, 138, 453- 60.
- Rubin BS, 2011. Bisphenol A: an endocrine disruptor with widespread exposure and multiple effects. J Steroid Biochem Mol Biol, 127(1-2), 27-34.
- Sadighara P, Khaniki GJ, Baseri E, Dehghani MH, et al., 2013. Effects of Bisphenol A on the quality charecteristics of meat in a chicken embryo model. Sci Int, 11(1), 375-8.
- Schultz E, McCormick KM, 1994. Skelatal muscle satellite cells. Rev Physiol Biochem Pharmacol, 23, 213-57.
- Sendón García RS, Losada PP, 2004. Determination of bisphenol A diglycidyl ether and its hydrolysis and chlorohydroxy derivates by liquid chromatography-mass spectrometry. J Chromatogr A, 1032 (1-2), 37-43.
- Stoloff L, Verret MJ, Dantzman J, Reynaldo EF, 1972. Toxicological study of aflatoxin P1 using the fertile chicken egg. Toxicol Appl Pharmacol, 23(3), 528-31.
- Sur E, Çelik İ, 2003. Effects of aflatoksin B1 on the development of bursa of Fabricius and blood lymphocyte acid phosphatase of the chicken. Br Poult Sci, 44(4), 558-66.
- Şişe Ş, 2011. Anne sütünde nonilfenol ve bisfenol a düzeylerinin belirlenmesi. Doktora Tezi, Afyon Kocatepe Üniversitesi Sağlık Bilimleri Enstitüsü, Afyon.
- Uscebrka G, Stojanovic S, Zikic D and Kanacki Z Gordana Uscebrka, Slobodan Stojanovic, Dragan Zikic and Zdenko Kanacki 2010. Morphodynamics of embryonic development of skeletal musculature of broiler and layer chickens. Avian Biology Research, 3 (4), 179-186.
- Vandenberg LN, Chahoud İ, Heindel JJ, Padmanabhan V, et al., 2010. Urinary, circulating, and tissue biomonitoring studies ındicate widespread exposure to bisphenol a. Environ Health Perspect, 118(8), 1055–70.
- Velleman SG, 2007. Muscle development in the embryo and hatchling. Poult Sci, 86(5),1050-4.
- Verrett MJ, Marliac J, McLaughlin J, 1964. Use of the chicken embryo in the assay of aflatoxin toxicity. J Assoc Off Anal Chem, 47(6), 1003-6.
- Wetherill YB, Petre CE, Monk KR, Puga A, et al., 2002. The xenoestrogen bisphenol A induces inappropriate androgen receptor activation and mitogenesis in prostatic adenocarcinoma cells . Mol Cancer Ther, 1(7), 515-524.
- Wiik A, Ekman M, Johansson O, Jansson E, et al., 2009. Expression of both oestrogen receptor alpha and beta in human skeletal muscle tissue. Histochem Cell Biol 131(2), 181–189.
- Yan H, Takamoto M, Sugane K, 2008. Exposure to Bisphenol A prenatally or in adulthood promotes TH2 cytokine production associated with reduction of CD4+CD25+ regulatory T cells. Environ Health Perspect, 116(4), 514-9.
- Yıldız N, 2009. Çevresel östrojenlerden bisfenol a ve oktilfenolün erkek sıçanlarda subkronik etkileri. Yüksek Lisans Tezi, Hacettepe Üniversitesi Fen Bilimleri Enstitüsü, Ankara.
- Yılmaz D, Öznurlu Y, 2019. Yumurtaya verilen bisfenol a’nın tavuklarda timusun gelişimi ve perifer kan alfa naftil asetat esteraz pozitif lenfosit oranı üzerindeki etkilerinin histolojik ve enzim histokimyasal yöntemlerle belirlenmesi. Eurasian J Vet Sci, 35(3), 144–151.
- Yiğit F, Aktaş A, Dağlıoğlu S, 2013. Effects of bisphenol a and diethylstilbestrol on the involution of bursa of fabricius in the hens. İstanbul Üniversitesi Veteriner Fakültesi Dergisi, 39(2), 168-74.
- Zielinska M, Sawosz E, Grodzik, M, Balcerak M, et al., 2012. Effect of taurine and gold nanoparticles on the morphological and molecular characteristics of muscle development during chicken embryogenesis. Arch Anim Nutr, 66(1), 1–13.
APA | Öznurlu Y, Ozaydın T, Sur E, KUŞAT T (2022). Yumurta sarısına enjekte edilen bisfenol A' nın tavuklarda iskelet kası gelişimi üzerindeki etkileri. , 90 - 100. 10.15312/EurasianJVetSci.2022.369 |
Chicago | Öznurlu Yasemin,Ozaydın Tugba,Sur Emrah,KUŞAT TANSU Yumurta sarısına enjekte edilen bisfenol A' nın tavuklarda iskelet kası gelişimi üzerindeki etkileri. (2022): 90 - 100. 10.15312/EurasianJVetSci.2022.369 |
MLA | Öznurlu Yasemin,Ozaydın Tugba,Sur Emrah,KUŞAT TANSU Yumurta sarısına enjekte edilen bisfenol A' nın tavuklarda iskelet kası gelişimi üzerindeki etkileri. , 2022, ss.90 - 100. 10.15312/EurasianJVetSci.2022.369 |
AMA | Öznurlu Y,Ozaydın T,Sur E,KUŞAT T Yumurta sarısına enjekte edilen bisfenol A' nın tavuklarda iskelet kası gelişimi üzerindeki etkileri. . 2022; 90 - 100. 10.15312/EurasianJVetSci.2022.369 |
Vancouver | Öznurlu Y,Ozaydın T,Sur E,KUŞAT T Yumurta sarısına enjekte edilen bisfenol A' nın tavuklarda iskelet kası gelişimi üzerindeki etkileri. . 2022; 90 - 100. 10.15312/EurasianJVetSci.2022.369 |
IEEE | Öznurlu Y,Ozaydın T,Sur E,KUŞAT T "Yumurta sarısına enjekte edilen bisfenol A' nın tavuklarda iskelet kası gelişimi üzerindeki etkileri." , ss.90 - 100, 2022. 10.15312/EurasianJVetSci.2022.369 |
ISNAD | Öznurlu, Yasemin vd. "Yumurta sarısına enjekte edilen bisfenol A' nın tavuklarda iskelet kası gelişimi üzerindeki etkileri". (2022), 90-100. https://doi.org/10.15312/EurasianJVetSci.2022.369 |
APA | Öznurlu Y, Ozaydın T, Sur E, KUŞAT T (2022). Yumurta sarısına enjekte edilen bisfenol A' nın tavuklarda iskelet kası gelişimi üzerindeki etkileri. Eurasian Journal of Veterinary Sciences, 38(2), 90 - 100. 10.15312/EurasianJVetSci.2022.369 |
Chicago | Öznurlu Yasemin,Ozaydın Tugba,Sur Emrah,KUŞAT TANSU Yumurta sarısına enjekte edilen bisfenol A' nın tavuklarda iskelet kası gelişimi üzerindeki etkileri. Eurasian Journal of Veterinary Sciences 38, no.2 (2022): 90 - 100. 10.15312/EurasianJVetSci.2022.369 |
MLA | Öznurlu Yasemin,Ozaydın Tugba,Sur Emrah,KUŞAT TANSU Yumurta sarısına enjekte edilen bisfenol A' nın tavuklarda iskelet kası gelişimi üzerindeki etkileri. Eurasian Journal of Veterinary Sciences, vol.38, no.2, 2022, ss.90 - 100. 10.15312/EurasianJVetSci.2022.369 |
AMA | Öznurlu Y,Ozaydın T,Sur E,KUŞAT T Yumurta sarısına enjekte edilen bisfenol A' nın tavuklarda iskelet kası gelişimi üzerindeki etkileri. Eurasian Journal of Veterinary Sciences. 2022; 38(2): 90 - 100. 10.15312/EurasianJVetSci.2022.369 |
Vancouver | Öznurlu Y,Ozaydın T,Sur E,KUŞAT T Yumurta sarısına enjekte edilen bisfenol A' nın tavuklarda iskelet kası gelişimi üzerindeki etkileri. Eurasian Journal of Veterinary Sciences. 2022; 38(2): 90 - 100. 10.15312/EurasianJVetSci.2022.369 |
IEEE | Öznurlu Y,Ozaydın T,Sur E,KUŞAT T "Yumurta sarısına enjekte edilen bisfenol A' nın tavuklarda iskelet kası gelişimi üzerindeki etkileri." Eurasian Journal of Veterinary Sciences, 38, ss.90 - 100, 2022. 10.15312/EurasianJVetSci.2022.369 |
ISNAD | Öznurlu, Yasemin vd. "Yumurta sarısına enjekte edilen bisfenol A' nın tavuklarda iskelet kası gelişimi üzerindeki etkileri". Eurasian Journal of Veterinary Sciences 38/2 (2022), 90-100. https://doi.org/10.15312/EurasianJVetSci.2022.369 |