Investigation of the Antioxidant, Antimicrobial, and Cytotoxic Activities of Endemic Marrubium rotundifolium Boiss
Yıl: 2022 Cilt: 28 Sayı: 1 Sayfa Aralığı: 45 - 51 Metin Dili: İngilizce DOI: 10.54614/eurjther.2022.0113 İndeks Tarihi: 21-06-2022
Investigation of the Antioxidant, Antimicrobial, and Cytotoxic Activities of Endemic Marrubium rotundifolium Boiss
Öz: Objective: Marrubium L. genus (Lamiaceae), which has 40 taxa in the world, is represented by 25 taxa in Turkey. Marrubium
rotundifolium Boiss. is an endemic species and is distributed in Aegean. This study was aimed to determine the antioxidant, antimicrobial, and cytotoxic effects of hexane, ethyl acetate, and methanol extracts of the endemic Marrubium rotundifolium Boiss.
Methods: Ultrasonic-assisted extraction was applied to aerial parts of the plant. The Folin-Ciocalteu and aluminum chloride/
potassium acetate methods determine the extract’s total phenolic and flavonoid contents. The 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+) radical decolorinaztion assay was used to determine the extracts’ Trolox equivalent antioxidant
capacity. The deoxyribose assay was carried out for determining the extracts’ OH− radical scavenging activity, and 2,2-diphenyl1-picrylhydrazyl was used for radical scavenging assay. 3-[4,5-Dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide method
was used to determine the in vitro cytotoxicity of plant extracts on 3 cancer (Caco-2, SH-SY5Y, and PC-3) and 2 non-cancer cell
lines (NIH-3T3 and HK-2). The antimicrobial activity was examined through the microdilution method.
Results: Phenolic contents of 2.62 ± 0.16 gallic acid equivalents μg/mL were observed in the methanol extract, while the highest
flavonoid content was determined in n-hexane extract (168.63 ± 2.76 QE μg/mL). In the OH˙−, ABTS˙+, and 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity studies, M. rotundifolium methanol extract showed higher IC50 values at lower doses (0.277
± 0.024, 3.21 ± 0.081, 0.033 ± 0.001 mg/mL, respectively). Concerning the cytotoxic activity, only methanol extract showed
inhibition on PC-3 cells (IC50: 0.173 ± 0.018 µg/mL). Minimum inhibitory concentrations of the extracts against Gram-positive
bacteria were lower than Gram-negative bacteria and yeast strains.
Conclusion: This study is the first detailed study that examines antioxidant and cytotoxic properties of endemic M. rotundifolium.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | AYDIN KÖSE F, ÖZTÜRK İ, Cin S, Baykan S (2022). Investigation of the Antioxidant, Antimicrobial, and Cytotoxic Activities of Endemic Marrubium rotundifolium Boiss. , 45 - 51. 10.54614/eurjther.2022.0113 |
| Chicago | AYDIN KÖSE Fadime,ÖZTÜRK İsmail,Cin Seden,Baykan Sura Investigation of the Antioxidant, Antimicrobial, and Cytotoxic Activities of Endemic Marrubium rotundifolium Boiss. (2022): 45 - 51. 10.54614/eurjther.2022.0113 |
| MLA | AYDIN KÖSE Fadime,ÖZTÜRK İsmail,Cin Seden,Baykan Sura Investigation of the Antioxidant, Antimicrobial, and Cytotoxic Activities of Endemic Marrubium rotundifolium Boiss. , 2022, ss.45 - 51. 10.54614/eurjther.2022.0113 |
| AMA | AYDIN KÖSE F,ÖZTÜRK İ,Cin S,Baykan S Investigation of the Antioxidant, Antimicrobial, and Cytotoxic Activities of Endemic Marrubium rotundifolium Boiss. . 2022; 45 - 51. 10.54614/eurjther.2022.0113 |
| Vancouver | AYDIN KÖSE F,ÖZTÜRK İ,Cin S,Baykan S Investigation of the Antioxidant, Antimicrobial, and Cytotoxic Activities of Endemic Marrubium rotundifolium Boiss. . 2022; 45 - 51. 10.54614/eurjther.2022.0113 |
| IEEE | AYDIN KÖSE F,ÖZTÜRK İ,Cin S,Baykan S "Investigation of the Antioxidant, Antimicrobial, and Cytotoxic Activities of Endemic Marrubium rotundifolium Boiss." , ss.45 - 51, 2022. 10.54614/eurjther.2022.0113 |
| ISNAD | AYDIN KÖSE, Fadime vd. "Investigation of the Antioxidant, Antimicrobial, and Cytotoxic Activities of Endemic Marrubium rotundifolium Boiss". (2022), 45-51. https://doi.org/10.54614/eurjther.2022.0113 |
| APA | AYDIN KÖSE F, ÖZTÜRK İ, Cin S, Baykan S (2022). Investigation of the Antioxidant, Antimicrobial, and Cytotoxic Activities of Endemic Marrubium rotundifolium Boiss. European Journal of Therapeutics, 28(1), 45 - 51. 10.54614/eurjther.2022.0113 |
| Chicago | AYDIN KÖSE Fadime,ÖZTÜRK İsmail,Cin Seden,Baykan Sura Investigation of the Antioxidant, Antimicrobial, and Cytotoxic Activities of Endemic Marrubium rotundifolium Boiss. European Journal of Therapeutics 28, no.1 (2022): 45 - 51. 10.54614/eurjther.2022.0113 |
| MLA | AYDIN KÖSE Fadime,ÖZTÜRK İsmail,Cin Seden,Baykan Sura Investigation of the Antioxidant, Antimicrobial, and Cytotoxic Activities of Endemic Marrubium rotundifolium Boiss. European Journal of Therapeutics, vol.28, no.1, 2022, ss.45 - 51. 10.54614/eurjther.2022.0113 |
| AMA | AYDIN KÖSE F,ÖZTÜRK İ,Cin S,Baykan S Investigation of the Antioxidant, Antimicrobial, and Cytotoxic Activities of Endemic Marrubium rotundifolium Boiss. European Journal of Therapeutics. 2022; 28(1): 45 - 51. 10.54614/eurjther.2022.0113 |
| Vancouver | AYDIN KÖSE F,ÖZTÜRK İ,Cin S,Baykan S Investigation of the Antioxidant, Antimicrobial, and Cytotoxic Activities of Endemic Marrubium rotundifolium Boiss. European Journal of Therapeutics. 2022; 28(1): 45 - 51. 10.54614/eurjther.2022.0113 |
| IEEE | AYDIN KÖSE F,ÖZTÜRK İ,Cin S,Baykan S "Investigation of the Antioxidant, Antimicrobial, and Cytotoxic Activities of Endemic Marrubium rotundifolium Boiss." European Journal of Therapeutics, 28, ss.45 - 51, 2022. 10.54614/eurjther.2022.0113 |
| ISNAD | AYDIN KÖSE, Fadime vd. "Investigation of the Antioxidant, Antimicrobial, and Cytotoxic Activities of Endemic Marrubium rotundifolium Boiss". European Journal of Therapeutics 28/1 (2022), 45-51. https://doi.org/10.54614/eurjther.2022.0113 |
