The expression profiles of NF-κB, Hsp70, and TNF-α in swine urinary bladder: an immunohistochemical study
Yıl: 2022 Cilt: 38 Sayı: 1 Sayfa Aralığı: 1 - 6 Metin Dili: İngilizce DOI: 10.15312/EurasianJVetSci.2022.358 İndeks Tarihi: 21-06-2022
The expression profiles of NF-κB, Hsp70, and TNF-α in swine urinary bladder: an immunohistochemical study
Öz: Aim: This study aimed to investigate the expression of NF-κB, Hsp70, and
TNF-α immunohistochemically in the swine urinary bladder.
Materials and Methods: A total of 8 swine urinary bladders were used as
material in the study. NF-κB, Hsp70 and TNF-α expression were demonstrated
by Streptavidin-biotin complex immunohistochemistry method.
Results: Uroepithelial cells gave a focal cytoplasmic reaction with NF-κB. A
moderate staining intensity was observed in NF-κB, smooth muscle cells of the
detrusor muscle layer and in the vessel wall. Hsp70 expression was observed
in the cytoplasms and nuclei of basal and intermediate cells. It was remarkable
that basal cell nuclei showed a strong reaction. The bladder's smooth muscle
bundles were stained positively with Hsp70. TNF-α expression was observed
throughout all three layers of urothelium as a brown color in the urinary
bladder. Interestingly, we determined that some umbrella cells' luminal
surfaces showed intense membrane-like immunopositive staining.
Conclusion: In this study, the expression of NF-κB, Hsp70, and TNF-α in the
swine urinary bladder was determined in epithelial cells of tunica mucosa and
smooth muscle cells of tunica muscularis. Based on the immunohistochemical
findings, it was concluded that the enzymes and cytokines secreted by the
cells forming the urothelium and detrusor muscle layer contribute to the
biological process, intercellular communication, physiological state and play
an important role in defense of the urinary bladder.
Anahtar Kelime: Domuz idrar kesesinde NF-κB, Hsp70 ve TNF-α'nın ekspresyon profilleri: immünohistokimyasal bir çalışma
Öz: Amaç: Bu çalışmada domuz idrar kesesinde immünohistokimyasal olarak NF-
κB, Hsp70 ve TNF-α ekspresyonunu araştırmayı amaçladık.
Gereç ve Yöntem: Çalışmada materyal olarak toplam 8 adet domuz idrar kesesi
kullanıldı. NF-κB, Hsp70 ve TNF-α ekspresyonu Streptavidin-biotin kompleks
immunohistokimya yöntemini ile gösterildi.
Bulgular: Üroepitelyal hücreler NF-κB ile fokal sitoplazmik reaksiyon verdi.
Detrusor kas katmanının düz kas hücrelerinde ve damar duvarında orta derecede
NF-κB boyanma yoğunluğu gözlendi. Bazal ve intermediyer hücrelerin
sitoplazmalarında ve çekirdeklerinde Hsp70 ekspresyonu gözlendi. Bazal hücre
çekirdeklerinin güçlü bir reaksiyon göstermesi dikkat çekiciydi. İdrar kesesinin
düz kas demetleri, Hsp70 ile pozitif olarak boyandı. TNF-α ekspresyonu,
idrar kesesinde kahverengi olarak ürotelyumun üç tabakasının tamamında
gözlendi. İlginç bir şekilde, bazı şemsiye hücrelerin lümen yüzeylerinin yoğun
membran benzeri immünopozitif boyama gösterdiğini belirledik.
Öneri: Bu çalışmada, domuz idrar kesesinde NF-κB, Hsp70 ve TNF-α ekspresyonu
tunika mukozanın epitel hücrelerinde ve tunica muscularisin düz kas
hücrelerinde belirlendi. İmmünhistokimyasal bulgulara dayanarak, ürotelyum
ve detrusor kas tabakasını oluşturan hücreler tarafından salgılanan enzimlerin
ve sitokinlerin biyolojik sürece, hücreler arası iletişime, fizyolojik duruma
katkıda bulunduğu ve idrar kesesinin savunmasında önemli bir rol oynadığı
sonucuna varıldı.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | ERTUĞRUL T, CEYLAN A (2022). The expression profiles of NF-κB, Hsp70, and TNF-α in swine urinary bladder: an immunohistochemical study. , 1 - 6. 10.15312/EurasianJVetSci.2022.358 |
| Chicago | ERTUĞRUL Tuğrul,CEYLAN AHMET The expression profiles of NF-κB, Hsp70, and TNF-α in swine urinary bladder: an immunohistochemical study. (2022): 1 - 6. 10.15312/EurasianJVetSci.2022.358 |
| MLA | ERTUĞRUL Tuğrul,CEYLAN AHMET The expression profiles of NF-κB, Hsp70, and TNF-α in swine urinary bladder: an immunohistochemical study. , 2022, ss.1 - 6. 10.15312/EurasianJVetSci.2022.358 |
| AMA | ERTUĞRUL T,CEYLAN A The expression profiles of NF-κB, Hsp70, and TNF-α in swine urinary bladder: an immunohistochemical study. . 2022; 1 - 6. 10.15312/EurasianJVetSci.2022.358 |
| Vancouver | ERTUĞRUL T,CEYLAN A The expression profiles of NF-κB, Hsp70, and TNF-α in swine urinary bladder: an immunohistochemical study. . 2022; 1 - 6. 10.15312/EurasianJVetSci.2022.358 |
| IEEE | ERTUĞRUL T,CEYLAN A "The expression profiles of NF-κB, Hsp70, and TNF-α in swine urinary bladder: an immunohistochemical study." , ss.1 - 6, 2022. 10.15312/EurasianJVetSci.2022.358 |
| ISNAD | ERTUĞRUL, Tuğrul - CEYLAN, AHMET. "The expression profiles of NF-κB, Hsp70, and TNF-α in swine urinary bladder: an immunohistochemical study". (2022), 1-6. https://doi.org/10.15312/EurasianJVetSci.2022.358 |
| APA | ERTUĞRUL T, CEYLAN A (2022). The expression profiles of NF-κB, Hsp70, and TNF-α in swine urinary bladder: an immunohistochemical study. Eurasian Journal of Veterinary Sciences, 38(1), 1 - 6. 10.15312/EurasianJVetSci.2022.358 |
| Chicago | ERTUĞRUL Tuğrul,CEYLAN AHMET The expression profiles of NF-κB, Hsp70, and TNF-α in swine urinary bladder: an immunohistochemical study. Eurasian Journal of Veterinary Sciences 38, no.1 (2022): 1 - 6. 10.15312/EurasianJVetSci.2022.358 |
| MLA | ERTUĞRUL Tuğrul,CEYLAN AHMET The expression profiles of NF-κB, Hsp70, and TNF-α in swine urinary bladder: an immunohistochemical study. Eurasian Journal of Veterinary Sciences, vol.38, no.1, 2022, ss.1 - 6. 10.15312/EurasianJVetSci.2022.358 |
| AMA | ERTUĞRUL T,CEYLAN A The expression profiles of NF-κB, Hsp70, and TNF-α in swine urinary bladder: an immunohistochemical study. Eurasian Journal of Veterinary Sciences. 2022; 38(1): 1 - 6. 10.15312/EurasianJVetSci.2022.358 |
| Vancouver | ERTUĞRUL T,CEYLAN A The expression profiles of NF-κB, Hsp70, and TNF-α in swine urinary bladder: an immunohistochemical study. Eurasian Journal of Veterinary Sciences. 2022; 38(1): 1 - 6. 10.15312/EurasianJVetSci.2022.358 |
| IEEE | ERTUĞRUL T,CEYLAN A "The expression profiles of NF-κB, Hsp70, and TNF-α in swine urinary bladder: an immunohistochemical study." Eurasian Journal of Veterinary Sciences, 38, ss.1 - 6, 2022. 10.15312/EurasianJVetSci.2022.358 |
| ISNAD | ERTUĞRUL, Tuğrul - CEYLAN, AHMET. "The expression profiles of NF-κB, Hsp70, and TNF-α in swine urinary bladder: an immunohistochemical study". Eurasian Journal of Veterinary Sciences 38/1 (2022), 1-6. https://doi.org/10.15312/EurasianJVetSci.2022.358 |
