Determination of Salmonella spp. presence and antibiotic resistance in egg and egg products
Yıl: 2022 Cilt: 38 Sayı: 1 Sayfa Aralığı: 24 - 31 Metin Dili: İngilizce DOI: 10.15312/EurasianJVetSci.2022.361 İndeks Tarihi: 21-06-2022
Determination of Salmonella spp. presence and antibiotic resistance in egg and egg products
Öz: Aim: The aim of this study was to determine the presence of Salmonella spp.
in egg and egg products and to determine antibiotic resistance profiles and
minimum inhibitory concentration (MIC) values of isolates.
Materials and Methods: A total of 200 samples including 100 eggs (35 village
eggs, 35 conventional eggs and 30 organic eggs) and 100 egg products (30
egg powders, 70 pasteurized liquid eggs) were collected from Samsun, Turkey.
Eggshell and egg contents samples were processed separately by pooling three
eggs together. The isolation and identification of Salmonella spp. was done according
to the method proposed by ISO 6579. The antimicrobial susceptibility
of Salmonella spp. isolates to various antibiotics and MIC values was performed
by VITEK 2 compact system using VITEK 2 AST-GN38 cards.
Results: Salmonella spp. were found in two of 100 (2%) eggs (organic egg
contents) and one of 100 (1%) egg products (pasteurized liquid egg). A total
of 11 isolates were confirmed by PCR techniques as Salmonella spp. with the
presence of oriC gene. The highest resistance was against amikacin, enrofloxacin,
gentamicin, tobramycin, cephalexin (100%), followed by nitrofurantoin
(81.8%), tetracycline (63.6%), ampicillin (54.5%), piperacillin (54.5%), cefpodoxime
(54.5%), and imipenem (9.09%). However, there was no resistance to
amoxicillin, marbofloxacin and trimethoprim/sulfamethoxazole. The results
showed that 11/11 (100%) of Salmonella spp. from three sample showed
multi-drug resistance against three or more antibiotic agents.
Conclusion: It is recommended to implement good hygiene practices, good
production practices and pasteurization techniques to minimize the risk of
Salmonellosis due to the consumption of eggs and egg products.
Anahtar Kelime: Yumurta ve Ürünlerinde Salmonella spp. varlığı ve antibiyotik dirençliliğinin belirlenmesi
Öz: Amaç: Bu çalışmanın amacı, yumurta ve yumurta ürünlerinde Salmonella spp
varlığını belirlemek ve elde edilen izolatların antibiyotik direnç profilleri ve
minimum inhibitör konsantrasyon (MİK) değerlerini belirlemektir.
Gereç ve Yöntem: Çalışmada 100 yumurta (35 köy yumurtası, 35 konvansiyonel
yumurta ve 30 organik yumurta) ve 100 yumurta ürünü (30 yumurta
tozu, 70 pastörize sıvı yumurta) olmak üzere toplam 200 numune Samsun,
Türkiye’den toplandı. Yumurta kabuğu ve yumurta içi örnekleri üç yumurta
bir araya getirilecek ayrı ayrı analiz edidi. Salmonella spp. izolasyon ve identifikasyonu
ISO 6579 tarafından önerilen yönteme göre yapıldı. Elde edilen Salmonella
spp. izolatlarının çeşitli antibiyotiklere karşı antimikrobiyal duyarlılığı
ve MİK değerleri VITEK 2 AST-GN38 kartları kullanılarak VITEK 2 kompakt
sistemi ile gerçekleştirildi.
Bulgular: İncelenen 100 yumurtanın (organik yumurta içi) ikisinde (%2) ve
100 yumurta ürününün (pastörize likit yumurta) birinde (%1) Salmonella
spp. pozitif bulundu. Toplam 11 izolat oriC geninin varlığı yönünden PCR tekniğiyle
Salmonella spp. olarak doğrulandı. Antibiyotik direnç testleri sonucunda
en yüksek direnç amikasin, enrofloksasin, gentamisin, tobramisin, sefaleksine
(%100), ardından nitrofurantoin (%81,8), tetrasiklin (%63.6), ampisilin
(%54,5), piperasilin (%54,5), sefpodoksim (%54,5), ve imipeneme (%9,09)
karşı bulundu. Bununla birlikte amoksisilin, marbofloksasin ve trimetoprim/
sülfametoksazole karşı direnç tespit edilemedi. Sonuçta üç numuneden elde
edilen 11 Salmonella spp. izolatının 11’inin (11/11, %100) üç veya daha fazla
antimikrobiyal ajana karşı çoklu direnç gösterdiği görüldü.
Öneri: Yumurta ve yumurta ürünlerinin tüketimine bağlı Salmonelloz riskini
en aza indirmek için iyi hijyen uygulamaları, iyi üretim uygulamaları ve pastörizasyon
tekniklerinin uygulanması önerilmektedir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | KESKİNOĞLU H, Terzi Gulel G (2022). Determination of Salmonella spp. presence and antibiotic resistance in egg and egg products. , 24 - 31. 10.15312/EurasianJVetSci.2022.361 |
| Chicago | KESKİNOĞLU HİLAL,Terzi Gulel Goknur Determination of Salmonella spp. presence and antibiotic resistance in egg and egg products. (2022): 24 - 31. 10.15312/EurasianJVetSci.2022.361 |
| MLA | KESKİNOĞLU HİLAL,Terzi Gulel Goknur Determination of Salmonella spp. presence and antibiotic resistance in egg and egg products. , 2022, ss.24 - 31. 10.15312/EurasianJVetSci.2022.361 |
| AMA | KESKİNOĞLU H,Terzi Gulel G Determination of Salmonella spp. presence and antibiotic resistance in egg and egg products. . 2022; 24 - 31. 10.15312/EurasianJVetSci.2022.361 |
| Vancouver | KESKİNOĞLU H,Terzi Gulel G Determination of Salmonella spp. presence and antibiotic resistance in egg and egg products. . 2022; 24 - 31. 10.15312/EurasianJVetSci.2022.361 |
| IEEE | KESKİNOĞLU H,Terzi Gulel G "Determination of Salmonella spp. presence and antibiotic resistance in egg and egg products." , ss.24 - 31, 2022. 10.15312/EurasianJVetSci.2022.361 |
| ISNAD | KESKİNOĞLU, HİLAL - Terzi Gulel, Goknur. "Determination of Salmonella spp. presence and antibiotic resistance in egg and egg products". (2022), 24-31. https://doi.org/10.15312/EurasianJVetSci.2022.361 |
| APA | KESKİNOĞLU H, Terzi Gulel G (2022). Determination of Salmonella spp. presence and antibiotic resistance in egg and egg products. Eurasian Journal of Veterinary Sciences, 38(1), 24 - 31. 10.15312/EurasianJVetSci.2022.361 |
| Chicago | KESKİNOĞLU HİLAL,Terzi Gulel Goknur Determination of Salmonella spp. presence and antibiotic resistance in egg and egg products. Eurasian Journal of Veterinary Sciences 38, no.1 (2022): 24 - 31. 10.15312/EurasianJVetSci.2022.361 |
| MLA | KESKİNOĞLU HİLAL,Terzi Gulel Goknur Determination of Salmonella spp. presence and antibiotic resistance in egg and egg products. Eurasian Journal of Veterinary Sciences, vol.38, no.1, 2022, ss.24 - 31. 10.15312/EurasianJVetSci.2022.361 |
| AMA | KESKİNOĞLU H,Terzi Gulel G Determination of Salmonella spp. presence and antibiotic resistance in egg and egg products. Eurasian Journal of Veterinary Sciences. 2022; 38(1): 24 - 31. 10.15312/EurasianJVetSci.2022.361 |
| Vancouver | KESKİNOĞLU H,Terzi Gulel G Determination of Salmonella spp. presence and antibiotic resistance in egg and egg products. Eurasian Journal of Veterinary Sciences. 2022; 38(1): 24 - 31. 10.15312/EurasianJVetSci.2022.361 |
| IEEE | KESKİNOĞLU H,Terzi Gulel G "Determination of Salmonella spp. presence and antibiotic resistance in egg and egg products." Eurasian Journal of Veterinary Sciences, 38, ss.24 - 31, 2022. 10.15312/EurasianJVetSci.2022.361 |
| ISNAD | KESKİNOĞLU, HİLAL - Terzi Gulel, Goknur. "Determination of Salmonella spp. presence and antibiotic resistance in egg and egg products". Eurasian Journal of Veterinary Sciences 38/1 (2022), 24-31. https://doi.org/10.15312/EurasianJVetSci.2022.361 |
