Ovarian drilling down-regulates endometrial nuclear
factor-κB p65 expression in women with PCOS:
A prospective case-control study

Objective: To investigate the impact of laparoscopic ovarian drilling (LOD) on the expression of endometrial NFκB p65 (Rel A) in women with clomiphene- resistant polycystic ovary syndrome (PCOS). Materials and Methods: The study group comprised 25 normal-weight women with PCOS undergoing LOD and 14 control women without PCOS. Endometrial NF-κB p65 levels evaluated before and after LOD following immunohistochemical staining. The semiquantitative method was used to evaluate the intensity of NF-κB p65 levels. NF-κB p65 was found to higher in the endometrium of patients with PCOS compared to controls. LOD leads to significant down-regulation in endometrial NF-κB p65 expression. NF-κB p65 expression of PCOS and fertile control were similar after LOD. After LOD, H-score values decreased approximately 3-fold. The H-score of the control subjects was lower than the preoperative and postoperative H-score values of the control women with ovarian cyst. Results: Expression of endometrial NF-κB p65 did not change following ovarian cystectomy. The laterality of the ovarian cyst did not cause any change in preoperative H-score values. Conclusion: By downregulating the endometrial NF-κB p65 expression LOD improved physiological inflammation in women with PCOS

Anahtar Kelime:

Brucellosis in pregnancy and its response to the changing immunoglobulin A: A prospective controlled study

Öz:

Objective: This study aimed to define the rare Brucella infection in pregnancy and its effects on immunoglobulins (Ig). Materials and Methods: This prospective study has conducted Brucella screening using the Rose Bengal test on pregnant and non-pregnant outpatients who did not show any specific Brucella symptoms. The immunoglobulin levels were measured using the enzyme-linked immunosorbent assay. The study group consisted of pregnant women who were at 20 weeks or below gestation and applied to our hospital outpatient clinic for routine check-ups. The control group consisted of healthy patients who applied for routine controls. Results: This study included a total of 584 participants, 293 of whom were controls and 291 were the study (pregnant) participants. The study revealed a 1.5% incidence of Brucella during pregnancy. In acute and chronic Brucella infection, lower levels of IgA response were observed in pregnant cases compared to the control group. Conclusion: Brucella infection is a disease that can cause fetal problems, especially in endemic areas. The role of the altered IgA response in pathologies that are associated with Brucella infection stands out as a new target for disease pathophysiology

Anahtar Kelime:

Gebelikte bruselloz ve değişen immünoglobülin A yanıtı: Prospektif kontrollü çalışma

Öz:

Amaç: Bu çalışma, gebelikte nadir görülen Brusella enfeksiyonunu ve immünoglobulinler (Ig) üzerine etkilerini belirlemek amacıyla planlandı. Gereç ve Yöntemler: Bu çalışma prospektif olarak planlandı. Bu amaçla, hastanemiz polikliniğine başvuran 20. gebelik haftası ve altı gebeler spesifik Brusella semptomlarına bakılmaksızın Rose Bengal testi ile tarandı. İmmünoglobulin seviyeleri enzim bağlı immünosorbent deneyi ile ölçüldü. Kontrol grubu rutin kontroller için başvuran sağlıklı hastalardan oluşturuldu. Bulgular: Bu çalışmaya 293’ü kontrol ve 291’i çalışma (gebe) olmak üzere toplam 584 katılımcı dahil edilmiştir. Çalışmada gebelikte Brusella görülme sıklığı %1,5 olarak bulunmuştur. Akut ve kronik Brusella enfeksiyonunda gebe olgularda kontrol grubuna göre daha düşük seviyelerde IgA yanıtı gözlendi. Sonuç: Brusella enfeksiyonu özellikle endemik bölgelerde fetal problemlere neden olabilen bir hastalıktır. Brusella enfeksiyonu ile ilişkili patolojilerde değişen IgA yanıtının rolü, hastalığın patofizyolojisi için yeni bir hedef olarak öne çıkmaktadır

Anahtar Kelime:

Ovaryen drilling PKOS hastalarında endometrial nükleer faktör κB p65 ekspresyonunu downregüle eder: Prospektif olgu kontrol çalışması

Öz:

Amaç: Bu çalışmanın amacı laparoskopik ovaryen drilling (LOD) işleminin klomifen sitrat rezistansı olan polikistik over sendromlu (PKOS) hastalarda endometriyal NFκB p65 (Rel A) ekspresyon düzeylerini etkileyip etkilemediğinin araştırılmasıdır. Gereç ve Yöntemler: Çalışma grubu LOD yapılan 25 tane normal kiloya sahip PKOS’li hastadan oluşmaktadır. Kontrol grubunda ise 7 tane PKOS olmayan sağlıklı fertil kadın ve 7 tane de benin kisti olan ancak endometriyoması olmayan toplam 14 hasta mevcuttur. Endometriyal NF-κB p65 ekspresyonu LOD öncesi ve sonrası mid-sekretuar fazda ölçülmüştür. Endometriyal örnekler immünohistokimyasal yöntemlerle boyanmıştır. Endometriyal NF-κB p65 ekspresyonu H-skor metodu ile değerlendirilmiştir Bulgular: Endometriyal NF-κB p65 ekspresyonu PKOS olan hastalarda LOD öncesi fertil hastalardan daha yüksek bulunmuştur. PKOS’li hastalarda LOD sonrası NF-κB p65 ekspresyonu belirgin olarak azalmıştır. LOD sonrası PKOS grubundaki hastalar ile fertil grupta NF-κB p65 ekspresyonu benzer bulunmuştur. LOD sonrası H-skoru 3 kat azalmıştır. Fertil grubun H-skoru pre-op ve post-op dönemde over kistli hasta grubunun H-skorundan düşük bulunmuştur. Yapılan benin kistektomi operasyonları sonrası endometrial NF-κB p65 ekspresyonunda belirgin değişiklik gösterilmemiştir. Ovaryen kist varlığı pre-operatif H-skor değerlerini etkilememektedir. Sonuç: LOD, klomifen sitrat rezistansı olan PKOS’li hastalarda endometrial NF-κB p65 ekspresyonunu azaltarak fizyolojik enflamasyonu ve reseptiviteyi iyileştirmektedir

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	dokuzeylul gungor n, Aydoğan kırmızı D, GÜNGÖR K, Baser E, yurci a, YESILYURT SOLEN E, Cil K, Kara M, hatirnaz s, Demir çaltekin M, Yilmaz N, Onat T, Yalvaç E (2022). Ovarian drilling down-regulates endometrial nuclear factor-κB p65 expression in women with PCOS: A prospective case-control study. , 45 - 50. 10.4274/tjod.galenos.2022.44845
Chicago	dokuzeylul gungor nur,Aydoğan kırmızı Demet,GÜNGÖR KAĞAN,Baser Emre,yurci arzu,YESILYURT SOLEN EMINE,Cil Kaan,Kara Mustafa,hatirnaz safak,Demir çaltekin Melike,Yilmaz Neziha,Onat Taylan,Yalvaç Ethem Serdar Ovarian drilling down-regulates endometrial nuclear factor-κB p65 expression in women with PCOS: A prospective case-control study. (2022): 45 - 50. 10.4274/tjod.galenos.2022.44845
MLA	dokuzeylul gungor nur,Aydoğan kırmızı Demet,GÜNGÖR KAĞAN,Baser Emre,yurci arzu,YESILYURT SOLEN EMINE,Cil Kaan,Kara Mustafa,hatirnaz safak,Demir çaltekin Melike,Yilmaz Neziha,Onat Taylan,Yalvaç Ethem Serdar Ovarian drilling down-regulates endometrial nuclear factor-κB p65 expression in women with PCOS: A prospective case-control study. , 2022, ss.45 - 50. 10.4274/tjod.galenos.2022.44845
AMA	dokuzeylul gungor n,Aydoğan kırmızı D,GÜNGÖR K,Baser E,yurci a,YESILYURT SOLEN E,Cil K,Kara M,hatirnaz s,Demir çaltekin M,Yilmaz N,Onat T,Yalvaç E Ovarian drilling down-regulates endometrial nuclear factor-κB p65 expression in women with PCOS: A prospective case-control study. . 2022; 45 - 50. 10.4274/tjod.galenos.2022.44845
Vancouver	dokuzeylul gungor n,Aydoğan kırmızı D,GÜNGÖR K,Baser E,yurci a,YESILYURT SOLEN E,Cil K,Kara M,hatirnaz s,Demir çaltekin M,Yilmaz N,Onat T,Yalvaç E Ovarian drilling down-regulates endometrial nuclear factor-κB p65 expression in women with PCOS: A prospective case-control study. . 2022; 45 - 50. 10.4274/tjod.galenos.2022.44845
IEEE	dokuzeylul gungor n,Aydoğan kırmızı D,GÜNGÖR K,Baser E,yurci a,YESILYURT SOLEN E,Cil K,Kara M,hatirnaz s,Demir çaltekin M,Yilmaz N,Onat T,Yalvaç E "Ovarian drilling down-regulates endometrial nuclear factor-κB p65 expression in women with PCOS: A prospective case-control study." , ss.45 - 50, 2022. 10.4274/tjod.galenos.2022.44845
ISNAD	dokuzeylul gungor, nur vd. "Ovarian drilling down-regulates endometrial nuclear factor-κB p65 expression in women with PCOS: A prospective case-control study". (2022), 45-50. https://doi.org/10.4274/tjod.galenos.2022.44845

APA	dokuzeylul gungor n, Aydoğan kırmızı D, GÜNGÖR K, Baser E, yurci a, YESILYURT SOLEN E, Cil K, Kara M, hatirnaz s, Demir çaltekin M, Yilmaz N, Onat T, Yalvaç E (2022). Ovarian drilling down-regulates endometrial nuclear factor-κB p65 expression in women with PCOS: A prospective case-control study. Turkish Journal of Obstetrics and Gynecology, 19(1), 45 - 50. 10.4274/tjod.galenos.2022.44845
Chicago	dokuzeylul gungor nur,Aydoğan kırmızı Demet,GÜNGÖR KAĞAN,Baser Emre,yurci arzu,YESILYURT SOLEN EMINE,Cil Kaan,Kara Mustafa,hatirnaz safak,Demir çaltekin Melike,Yilmaz Neziha,Onat Taylan,Yalvaç Ethem Serdar Ovarian drilling down-regulates endometrial nuclear factor-κB p65 expression in women with PCOS: A prospective case-control study. Turkish Journal of Obstetrics and Gynecology 19, no.1 (2022): 45 - 50. 10.4274/tjod.galenos.2022.44845
MLA	dokuzeylul gungor nur,Aydoğan kırmızı Demet,GÜNGÖR KAĞAN,Baser Emre,yurci arzu,YESILYURT SOLEN EMINE,Cil Kaan,Kara Mustafa,hatirnaz safak,Demir çaltekin Melike,Yilmaz Neziha,Onat Taylan,Yalvaç Ethem Serdar Ovarian drilling down-regulates endometrial nuclear factor-κB p65 expression in women with PCOS: A prospective case-control study. Turkish Journal of Obstetrics and Gynecology, vol.19, no.1, 2022, ss.45 - 50. 10.4274/tjod.galenos.2022.44845
AMA	dokuzeylul gungor n,Aydoğan kırmızı D,GÜNGÖR K,Baser E,yurci a,YESILYURT SOLEN E,Cil K,Kara M,hatirnaz s,Demir çaltekin M,Yilmaz N,Onat T,Yalvaç E Ovarian drilling down-regulates endometrial nuclear factor-κB p65 expression in women with PCOS: A prospective case-control study. Turkish Journal of Obstetrics and Gynecology. 2022; 19(1): 45 - 50. 10.4274/tjod.galenos.2022.44845
Vancouver	dokuzeylul gungor n,Aydoğan kırmızı D,GÜNGÖR K,Baser E,yurci a,YESILYURT SOLEN E,Cil K,Kara M,hatirnaz s,Demir çaltekin M,Yilmaz N,Onat T,Yalvaç E Ovarian drilling down-regulates endometrial nuclear factor-κB p65 expression in women with PCOS: A prospective case-control study. Turkish Journal of Obstetrics and Gynecology. 2022; 19(1): 45 - 50. 10.4274/tjod.galenos.2022.44845
IEEE	dokuzeylul gungor n,Aydoğan kırmızı D,GÜNGÖR K,Baser E,yurci a,YESILYURT SOLEN E,Cil K,Kara M,hatirnaz s,Demir çaltekin M,Yilmaz N,Onat T,Yalvaç E "Ovarian drilling down-regulates endometrial nuclear factor-κB p65 expression in women with PCOS: A prospective case-control study." Turkish Journal of Obstetrics and Gynecology, 19, ss.45 - 50, 2022. 10.4274/tjod.galenos.2022.44845
ISNAD	dokuzeylul gungor, nur vd. "Ovarian drilling down-regulates endometrial nuclear factor-κB p65 expression in women with PCOS: A prospective case-control study". Turkish Journal of Obstetrics and Gynecology 19/1 (2022), 45-50. https://doi.org/10.4274/tjod.galenos.2022.44845