Sıçanlarada kimyasal simpatektominin nörotoksik etkisi

VAROL, TUNCAY; YAVAŞOĞLU, Altuğ; Tuğlu, Mehmet İbrahim; TUÇ YÜCEL, AYSE

Sıçanlarada kimyasal simpatektominin nörotoksik etkisi

AYŞE TUÇ YÜCEL, (Celal Bayar Üniversitesi, Sağlık Hizmetleri Meslek Yüksek Okulu Manisa, Türkiye)

İbrahim TUĞLU, (Celal Bayar Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Manisa, Türkiye)

Altuğ YAVAŞOĞLU, (Ege Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, İzmir, Türkiye)

Tuncay VAROL (Celal Bayar Üniversitesi, Tıp Fakültesi, Anatomi Anabilim Dalı, Manisa, Türkiye)

Ege Tıp Dergisi

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Yıl: 2006 Cilt: 45 Sayı: 1 Sayfa Aralığı: 25 - 30 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Sıçanlarada kimyasal simpatektominin nörotoksik etkisi

Öz:

Amaç: Guanethidine bir antihipertansif ilaç olup, beyin damarlarında yapısal değişikliklere neden olabilen yan etkileri vardır. Deneysel çalışmalarda kimyasal simpatektomi yapmak için kullanılmaktadır. Her iki durumda da özellikle etki alanları dışında nörotoksik etkisi ile ilgili bilgi bulunmamaktadır. Bu nedenle nörotoksik etkiye en duyarlı beyin bölgelerinde, histolojik yöntemle guanethidine etkisinin araştırılması amaçlandı. Gereç ve Yöntem: Erişkin sıçanlarda intraperitoneal olarak 10 mg/kg guanethidine ve kontrol grubunda aynı volümde serum fizyolojik (SF) 30 gün süre île tek doz uygulandı. Süre sonunda anestezi ile dekapite edilen denekler, kardiyak perfüzyon sonrasında beyinleri çıkartılarak parafine gömüldü. Histolojik kesitler ışık mikroskobu ile incelendi. Bulgular: SF verilen grupta toksik etkiye duyarlı truncus cerebri, cerebellum, cortex cerebri ve hippocampus alanlarında hiçbir patolojik değişikliğe rastlanmazken, Guanethidine uygulanan grupta bazı alanlarda hücre çevresindeki boşlukların arttığı, nükleusların kondanse olduğu, piknosize benzer yapının oluştuğu gözlendi. Sonuç: Guanethidine yan etkileri nedeniyle dikkatle kullanılması gereken bir ajan olarak klinikte ve olası nörotoksik etkisi nedeniyle de deneysel çalışmalarda sonuçların yorumlanmasında sorun oluşturabilecek potansiyele sahip gözükmektedir. Etki alanları dışında daha önce nörotoksik etkisi araştırılmamış bu maddenin, toksisitesi ile ilgili daha detaylı çalışmaların yapılması yararlı olacaktır.

Anahtar Kelime: Nörotoksisite sendromları Sempatektomi, kimyasal Guanetidin Histoloji Sıçan, Wistar

Konular: Biyokimya ve Moleküler Biyoloji

The neurotoxic effect of chemical sympathectomy in the rat

Öz:

Objective: Guanethidine is an antihypertensive drug which can produce structural alterations on the vessels of the brain. It has also been used for the purpose of chemical sympathectomy in experimental studies. However there is little, if any, information on the neurotoxic effects of the drug except the regions where they exert their primary effects. Therefore we have conducted this study to histologically investigate the effects of guanethidine in regions most susceptible to neurotoxicity. Material and Methods: Desympathization was induced in mature rats by daily single intraperitoneal injection of 10 mg/kg of guanethidine for 30 days. Same volume of saline was injected the same way to control animals. Rats were euthanized with cardiac perfusion and brains were removed to be fixed in formaline and embedded in paraffin. Serial sections were stained with H&E and examined under light microscope.Cell density was measured from selected vulnarable regions of the brain by morphometric methods. Findings: We fo an increase in pericytoplasmic area, nuclear condensation and picnosis-like changes in guanethidine group. There w no clear pathologic alterations in the brain stem, cerebellum, cerebral cortex and hippocampus in the control grc Results and Conclusion: Guanethidine needs to be used with caution clinically due to its side effects and experiment due to its possible neurotoxic effects which may confound interpretation of the findings. Further studies are requirei uncover the neurotoxic effects of the drug.

Anahtar Kelime: Guanethidine Histology Rats, Wistar Neurotoxicity Syndromes Sympathectomy, Chemical

Konular: Biyokimya ve Moleküler Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	TUÇ YÜCEL A, Tuğlu M, YAVAŞOĞLU A, VAROL T (2006). Sıçanlarada kimyasal simpatektominin nörotoksik etkisi. , 25 - 30.
Chicago	TUÇ YÜCEL AYSE,Tuğlu Mehmet İbrahim,YAVAŞOĞLU Altuğ,VAROL TUNCAY Sıçanlarada kimyasal simpatektominin nörotoksik etkisi. (2006): 25 - 30.
MLA	TUÇ YÜCEL AYSE,Tuğlu Mehmet İbrahim,YAVAŞOĞLU Altuğ,VAROL TUNCAY Sıçanlarada kimyasal simpatektominin nörotoksik etkisi. , 2006, ss.25 - 30.
AMA	TUÇ YÜCEL A,Tuğlu M,YAVAŞOĞLU A,VAROL T Sıçanlarada kimyasal simpatektominin nörotoksik etkisi. . 2006; 25 - 30.
Vancouver	TUÇ YÜCEL A,Tuğlu M,YAVAŞOĞLU A,VAROL T Sıçanlarada kimyasal simpatektominin nörotoksik etkisi. . 2006; 25 - 30.
IEEE	TUÇ YÜCEL A,Tuğlu M,YAVAŞOĞLU A,VAROL T "Sıçanlarada kimyasal simpatektominin nörotoksik etkisi." , ss.25 - 30, 2006.
ISNAD	TUÇ YÜCEL, AYSE vd. "Sıçanlarada kimyasal simpatektominin nörotoksik etkisi". (2006), 25-30.

APA	TUÇ YÜCEL A, Tuğlu M, YAVAŞOĞLU A, VAROL T (2006). Sıçanlarada kimyasal simpatektominin nörotoksik etkisi. Ege Tıp Dergisi, 45(1), 25 - 30.
Chicago	TUÇ YÜCEL AYSE,Tuğlu Mehmet İbrahim,YAVAŞOĞLU Altuğ,VAROL TUNCAY Sıçanlarada kimyasal simpatektominin nörotoksik etkisi. Ege Tıp Dergisi 45, no.1 (2006): 25 - 30.
MLA	TUÇ YÜCEL AYSE,Tuğlu Mehmet İbrahim,YAVAŞOĞLU Altuğ,VAROL TUNCAY Sıçanlarada kimyasal simpatektominin nörotoksik etkisi. Ege Tıp Dergisi, vol.45, no.1, 2006, ss.25 - 30.
AMA	TUÇ YÜCEL A,Tuğlu M,YAVAŞOĞLU A,VAROL T Sıçanlarada kimyasal simpatektominin nörotoksik etkisi. Ege Tıp Dergisi. 2006; 45(1): 25 - 30.
Vancouver	TUÇ YÜCEL A,Tuğlu M,YAVAŞOĞLU A,VAROL T Sıçanlarada kimyasal simpatektominin nörotoksik etkisi. Ege Tıp Dergisi. 2006; 45(1): 25 - 30.
IEEE	TUÇ YÜCEL A,Tuğlu M,YAVAŞOĞLU A,VAROL T "Sıçanlarada kimyasal simpatektominin nörotoksik etkisi." Ege Tıp Dergisi, 45, ss.25 - 30, 2006.
ISNAD	TUÇ YÜCEL, AYSE vd. "Sıçanlarada kimyasal simpatektominin nörotoksik etkisi". Ege Tıp Dergisi 45/1 (2006), 25-30.