Evidence for the presence of a second electron donor for the cytoplasmic thioredowins in the yeast Saccharomyces cerevisiae
Yıl: 2006 Cilt: 30 Sayı: 3 Sayfa Aralığı: 133 - 138 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022
Evidence for the presence of a second electron donor for the cytoplasmic thioredowins in the yeast Saccharomyces cerevisiae
Öz: Maya mantarında sitoplazmik tioredoksin sistemi NADPH, bir tane tiyoredoksin redüktaz (TRR1) ve iki tane tiyoredoksin (TRX1, TRXZ) den oluşmaktadır. Bu çalışmada, genomundan TRR1, TRXl.ve TRXZ genleri çıkartılmış maya hücrelerinin metiyonin sülfoksit indirgeme kapasiteleri incelenmiştir. İki tiyoredoksini aynı anda çıkartılmış mutant hücreler metiyonin sulfoksiti indirgeyip metiyonin oluşturamazken, tiyoredoksin redüktaz-1 geni çıkartılmış hücreler oluşturabildiler. Bu maya mantarında metiyonin sülfoksit redüktazlar için gerekli olan elektronların tiyoredoksinlerden sağlandığını gösteren ilk çalışmadır. Ayrıca metiyonin sülfoksit indirgenmesi için gerekli olan elektronlar tiyoredoksin redüktaz-1 olmadan NADPH den tiyoredoksine transfer edilebildiler. Aynı sonuç sülfatın indirgenip methiyonin sentezinde kullanılmasında rol oynayan 3-fosfoadenozin 5-fosfosülfat redüktaz enzim aktivitesi içinde gözlemlendi. Her iki deneyin sonuçlarına göre, tiyoredoksin redüktaz-1 in yokluğunda tiyoredoksinler için gerekli olan elektronlar kimliği bilinmeyen ikinci bir elektron donörü tarafından sağlanmaktadır.
Anahtar Kelime: Konular:
Maya (Saccharomyces cerevisiae) Sitoplazmik tiyoredoksinler iiçin ikinci bir elektron donörününvarlığını göjsteren delilller
Öz: In yeast, the cytoplasmic thioredoxin system is composed of NADPH, thioredoxin reductase-1 {TRR1) and 2 thioredoxin genes (TRX1, TRXZ). In this study, using yeast knockout mutants for TRR1, TRX1 and TRX2 genes, the role of the thioredoxin system in methionine sulfoxide reduction was investigated. Cells lacking both TRX1 and TRXZ genes simultaneously were not able to reduce methionine sulfoxides to methionine; however, mutants missing the TRR1 gene were able to reduce methionine sulfoxides to methionine, which showed that electrons could be transferred from NADPH to thioredoxins in the absence of TRR1. Similar results were observed for 3-phosphoadenosine 5-phosphosulfate reduction in the inorganic sulfate assimilation pathway. Results from both assays suggested that yeast cells have additional cytoplasmic thioredoxin reductase activity that could compensate for methionine sulfoxide reduction and sulfate assimilation in the absence of TRR1. This report also constitutes the first evidence that thioredoxins are the in vivo electron donors for methionine sulfoxide reductases in yeast.
Anahtar Kelime: Konular:
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Koc A, KARAKAYA Ç, ÜNLÜ E (2006). Evidence for the presence of a second electron donor for the cytoplasmic thioredowins in the yeast Saccharomyces cerevisiae. , 133 - 138. |
| Chicago | Koc Ahmet,KARAKAYA Ç.,ÜNLÜ Ercan S. Evidence for the presence of a second electron donor for the cytoplasmic thioredowins in the yeast Saccharomyces cerevisiae. (2006): 133 - 138. |
| MLA | Koc Ahmet,KARAKAYA Ç.,ÜNLÜ Ercan S. Evidence for the presence of a second electron donor for the cytoplasmic thioredowins in the yeast Saccharomyces cerevisiae. , 2006, ss.133 - 138. |
| AMA | Koc A,KARAKAYA Ç,ÜNLÜ E Evidence for the presence of a second electron donor for the cytoplasmic thioredowins in the yeast Saccharomyces cerevisiae. . 2006; 133 - 138. |
| Vancouver | Koc A,KARAKAYA Ç,ÜNLÜ E Evidence for the presence of a second electron donor for the cytoplasmic thioredowins in the yeast Saccharomyces cerevisiae. . 2006; 133 - 138. |
| IEEE | Koc A,KARAKAYA Ç,ÜNLÜ E "Evidence for the presence of a second electron donor for the cytoplasmic thioredowins in the yeast Saccharomyces cerevisiae." , ss.133 - 138, 2006. |
| ISNAD | Koc, Ahmet vd. "Evidence for the presence of a second electron donor for the cytoplasmic thioredowins in the yeast Saccharomyces cerevisiae". (2006), 133-138. |
| APA | Koc A, KARAKAYA Ç, ÜNLÜ E (2006). Evidence for the presence of a second electron donor for the cytoplasmic thioredowins in the yeast Saccharomyces cerevisiae. Turkish Journal of Biology, 30(3), 133 - 138. |
| Chicago | Koc Ahmet,KARAKAYA Ç.,ÜNLÜ Ercan S. Evidence for the presence of a second electron donor for the cytoplasmic thioredowins in the yeast Saccharomyces cerevisiae. Turkish Journal of Biology 30, no.3 (2006): 133 - 138. |
| MLA | Koc Ahmet,KARAKAYA Ç.,ÜNLÜ Ercan S. Evidence for the presence of a second electron donor for the cytoplasmic thioredowins in the yeast Saccharomyces cerevisiae. Turkish Journal of Biology, vol.30, no.3, 2006, ss.133 - 138. |
| AMA | Koc A,KARAKAYA Ç,ÜNLÜ E Evidence for the presence of a second electron donor for the cytoplasmic thioredowins in the yeast Saccharomyces cerevisiae. Turkish Journal of Biology. 2006; 30(3): 133 - 138. |
| Vancouver | Koc A,KARAKAYA Ç,ÜNLÜ E Evidence for the presence of a second electron donor for the cytoplasmic thioredowins in the yeast Saccharomyces cerevisiae. Turkish Journal of Biology. 2006; 30(3): 133 - 138. |
| IEEE | Koc A,KARAKAYA Ç,ÜNLÜ E "Evidence for the presence of a second electron donor for the cytoplasmic thioredowins in the yeast Saccharomyces cerevisiae." Turkish Journal of Biology, 30, ss.133 - 138, 2006. |
| ISNAD | Koc, Ahmet vd. "Evidence for the presence of a second electron donor for the cytoplasmic thioredowins in the yeast Saccharomyces cerevisiae". Turkish Journal of Biology 30/3 (2006), 133-138. |
