Preparation of bentonite-cysteine (Bent-Cys) Micro-composite affinity sorbents for ferritin adsorption

Denizli, Adil; TABAK, Ahmet; AKGÖL, Sinan; ÖZTÜRK, Nevra; Uzun, Lokman

Preparation of bentonite-cysteine (Bent-Cys) Micro-composite affinity sorbents for ferritin adsorption

Nevra ÖZTÜRK, (Adnan Menderes Üniversitesi, Kimya Bölümü, Aydın, Türkiye)

Ahmet TABAK, (Rize Üniversitesi, Kimya Bölümü, Rize, Türkiye)

Sinan AKGÖL, (Adnan Menderes Üniversitesi, Kimya Bölümü, Aydın, Türkiye)

Lokman UZUN, (Hacettepe Üniversitesi, Kimya Bölümü, Ankara, Türkiye)

Adil DENİZLİ (Hacettepe Üniversitesi, Kimya Bölümü, Ankara, Türkiye)

Hacettepe Journal of Biology and Chemistry

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Yıl: 2007 Cilt: 35 Sayı: 1 Sayfa Aralığı: 67 - 76 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Preparation of bentonite-cysteine (Bent-Cys) Micro-composite affinity sorbents for ferritin adsorption

Öz:

his study focused on developing bentonite-cysteine micro-composite «affinity sorbents (Bent-Cys) (38-105 |am) for Ferritin adsorption from aqueous solutions. The pseudo-specific affinity ligand L-cysteine was immobilized by covalent binding onto the bentonite structure. X-ray diffraction and FTIR analysis of Bent-Cys composite affinity sorbents were performed. The specific surface area of the bentonite and Bent-Cys micro-composite structures were found to be 33.0 and^2.8 m2/g, respectively. Elemental analysis of immobilized L-cysteine for nitrogen was estimated to be 541.3 |j.mol/g bentonite. The non-specific ferritin adsorption onto bentonite structure was negligible (ca. 0.9 mg/g). Higher adsorption values up to 40.0 mg/g were obtained in which Bent-Cys micro-composite affinity sorbents were used from aqueous solutions. Adsorption studies were performed to evaluate the effect of initial concentration of ferritin, pH, temperature. The desorption characteristics of Bent-Cys micro-composite affinity sorbents were also studied. The maximum amount of ferritin adsorption from aqueous solution was observed at pH 4.0. Ferritin molecules could be repeatedly adsorbed and desorbed with the micro-composite affinity sorbent system without noticeable loss in the ferritin adsorption capacity.

Anahtar Kelime: bentonite ferritin cysteine chromatography adsorption

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	ÖZTÜRK N, TABAK A, AKGÖL S, Uzun L, Denizli A (2007). Preparation of bentonite-cysteine (Bent-Cys) Micro-composite affinity sorbents for ferritin adsorption. , 67 - 76.
Chicago	ÖZTÜRK Nevra,TABAK Ahmet,AKGÖL Sinan,Uzun Lokman,Denizli Adil Preparation of bentonite-cysteine (Bent-Cys) Micro-composite affinity sorbents for ferritin adsorption. (2007): 67 - 76.
MLA	ÖZTÜRK Nevra,TABAK Ahmet,AKGÖL Sinan,Uzun Lokman,Denizli Adil Preparation of bentonite-cysteine (Bent-Cys) Micro-composite affinity sorbents for ferritin adsorption. , 2007, ss.67 - 76.
AMA	ÖZTÜRK N,TABAK A,AKGÖL S,Uzun L,Denizli A Preparation of bentonite-cysteine (Bent-Cys) Micro-composite affinity sorbents for ferritin adsorption. . 2007; 67 - 76.
Vancouver	ÖZTÜRK N,TABAK A,AKGÖL S,Uzun L,Denizli A Preparation of bentonite-cysteine (Bent-Cys) Micro-composite affinity sorbents for ferritin adsorption. . 2007; 67 - 76.
IEEE	ÖZTÜRK N,TABAK A,AKGÖL S,Uzun L,Denizli A "Preparation of bentonite-cysteine (Bent-Cys) Micro-composite affinity sorbents for ferritin adsorption." , ss.67 - 76, 2007.
ISNAD	ÖZTÜRK, Nevra vd. "Preparation of bentonite-cysteine (Bent-Cys) Micro-composite affinity sorbents for ferritin adsorption". (2007), 67-76.

APA	ÖZTÜRK N, TABAK A, AKGÖL S, Uzun L, Denizli A (2007). Preparation of bentonite-cysteine (Bent-Cys) Micro-composite affinity sorbents for ferritin adsorption. Hacettepe Journal of Biology and Chemistry, 35(1), 67 - 76.
Chicago	ÖZTÜRK Nevra,TABAK Ahmet,AKGÖL Sinan,Uzun Lokman,Denizli Adil Preparation of bentonite-cysteine (Bent-Cys) Micro-composite affinity sorbents for ferritin adsorption. Hacettepe Journal of Biology and Chemistry 35, no.1 (2007): 67 - 76.
MLA	ÖZTÜRK Nevra,TABAK Ahmet,AKGÖL Sinan,Uzun Lokman,Denizli Adil Preparation of bentonite-cysteine (Bent-Cys) Micro-composite affinity sorbents for ferritin adsorption. Hacettepe Journal of Biology and Chemistry, vol.35, no.1, 2007, ss.67 - 76.
AMA	ÖZTÜRK N,TABAK A,AKGÖL S,Uzun L,Denizli A Preparation of bentonite-cysteine (Bent-Cys) Micro-composite affinity sorbents for ferritin adsorption. Hacettepe Journal of Biology and Chemistry. 2007; 35(1): 67 - 76.
Vancouver	ÖZTÜRK N,TABAK A,AKGÖL S,Uzun L,Denizli A Preparation of bentonite-cysteine (Bent-Cys) Micro-composite affinity sorbents for ferritin adsorption. Hacettepe Journal of Biology and Chemistry. 2007; 35(1): 67 - 76.
IEEE	ÖZTÜRK N,TABAK A,AKGÖL S,Uzun L,Denizli A "Preparation of bentonite-cysteine (Bent-Cys) Micro-composite affinity sorbents for ferritin adsorption." Hacettepe Journal of Biology and Chemistry, 35, ss.67 - 76, 2007.
ISNAD	ÖZTÜRK, Nevra vd. "Preparation of bentonite-cysteine (Bent-Cys) Micro-composite affinity sorbents for ferritin adsorption". Hacettepe Journal of Biology and Chemistry 35/1 (2007), 67-76.