The biochemical basis of insecticide resistance and determination of esterase enzyme patterns by using page in field collected populations of Drosophila melanogaster from Muğla province of Turkey

TALKIN, Vatan; ARSLAN, Tülin; ÇÖL, Bekir; KÜÇÜKAKYÜZ, KÖKSAL; Göçmen Taşkın, Belgin

The biochemical basis of insecticide resistance and determination of esterase enzyme patterns by using page in field collected populations of Drosophila melanogaster from Muğla province of Turkey

Vatan TALKIN, (Muğla Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Muğla, Türkiye)

Köksal KÜÇÜKAKYÜZ, (Muğla Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Muğla, Türkiye)

Tülin ARSLAN, (Muğla Üniversitesi, Su Ürünleri Fakültesi, Muğla, Türkiye)

Bekir ÇÖL, (Muğla Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Muğla, Türkiye)

BELGİN GÖÇMEN TAŞKIN (Muğla Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Muğla, Türkiye)

Journal of Cell and Molecular Biology

11 0

Yıl: 2007 Cilt: 6 Sayı: 1 Sayfa Aralığı: 31 - 40 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

The biochemical basis of insecticide resistance and determination of esterase enzyme patterns by using page in field collected populations of Drosophila melanogaster from Muğla province of Turkey

Öz:

Biyokimyasal ve moleküler biyoloji teknikleri, doğal populasyonlarda, potansiyel veya gelişmekte olan direnç mekanizmaları hakkında çok spesifik bilgiler sağlamaktadır. Bu araştırmada, Muğla ve ilçelerinden toplanan Drosophila melanogaster populasyonlarında glutatyon-S-transferaz (GST), yüzde kalan asetilkolin esteraz (AChE) ve genel esteraz aktiviteleri ölçülmüştür. Bununla birlikte poliakrilamid jel elektroforezi (PAGE) yöntemi kullanılarak örneklerdeki esteraz enzim profilleri belirlenmiştir. Yüzde kalan asetilkolin esteraz enzim aktivitesi incelenen örneklerde duyarlı soya göre yüksek bulunmuştur, a ve (3-naftil asetatın substrat olarak kullanılması ile toplam 21 esteraz bandı elde edilmiştir, bu bantların 18 tanesi a esteraz, iki tanesi (3 esteraz ve bir tanesi ise a/B esteraz olarak sınıflandırılmıştır.

Anahtar Kelime: PAGE Drosophila melanogaster böcek öldürücü direnci enzim aktivitesi Muğla esteraz glutatyon transferaz glutatyon S-transferaz asetilkolinesteraz

Konular: Biyokimya ve Moleküler Biyoloji

Muğla ve ilçelerine ait Drosophila melanogaster populasyonlarında insektisit direncinin biyokimyasal temellerinin ve esteraz enzim profilinin PAGE yšntemi kullanılarak belirlenmesi

Öz:

Biochemical and molecular biology methods provide more specific information about the resistance potential or the development of resistance in field populations of living organisms. In this research, the enzymatic activities of Glutathione-S-transferase (GST), percent remaining acetylcholinesterase (AChE) and general esterase were measured in Drosophila melanogaster populations collected from different towns of Muğla Province. Furthermore, esterase enzyme patterns of these populations were determined by using polyacrylamide gel electrophoresis (PAGE). Percent remaining activities of AChE were found to be significantly higher in all strains compared to the activities of the susceptible one. Twenty one different esterase bands were detected when a- and (3-naphtyl acetate were used as substrates and the bands were classified as 18 a-esterases, 2 (3-esterases and 1 a/(3 esterase.

Anahtar Kelime: Drosophila melanogaster insecticide resistance enzyme activity Mugla esterases glutathione transferase glutathione S-transferase acetylcholinesterase PAGE

Konular: Biyokimya ve Moleküler Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	TALKIN V, KÜÇÜKAKYÜZ K, ARSLAN T, ÇÖL B, Göçmen Taşkın B (2007). The biochemical basis of insecticide resistance and determination of esterase enzyme patterns by using page in field collected populations of Drosophila melanogaster from Muğla province of Turkey. , 31 - 40.
Chicago	TALKIN Vatan,KÜÇÜKAKYÜZ KÖKSAL,ARSLAN Tülin,ÇÖL Bekir,Göçmen Taşkın Belgin The biochemical basis of insecticide resistance and determination of esterase enzyme patterns by using page in field collected populations of Drosophila melanogaster from Muğla province of Turkey. (2007): 31 - 40.
MLA	TALKIN Vatan,KÜÇÜKAKYÜZ KÖKSAL,ARSLAN Tülin,ÇÖL Bekir,Göçmen Taşkın Belgin The biochemical basis of insecticide resistance and determination of esterase enzyme patterns by using page in field collected populations of Drosophila melanogaster from Muğla province of Turkey. , 2007, ss.31 - 40.
AMA	TALKIN V,KÜÇÜKAKYÜZ K,ARSLAN T,ÇÖL B,Göçmen Taşkın B The biochemical basis of insecticide resistance and determination of esterase enzyme patterns by using page in field collected populations of Drosophila melanogaster from Muğla province of Turkey. . 2007; 31 - 40.
Vancouver	TALKIN V,KÜÇÜKAKYÜZ K,ARSLAN T,ÇÖL B,Göçmen Taşkın B The biochemical basis of insecticide resistance and determination of esterase enzyme patterns by using page in field collected populations of Drosophila melanogaster from Muğla province of Turkey. . 2007; 31 - 40.
IEEE	TALKIN V,KÜÇÜKAKYÜZ K,ARSLAN T,ÇÖL B,Göçmen Taşkın B "The biochemical basis of insecticide resistance and determination of esterase enzyme patterns by using page in field collected populations of Drosophila melanogaster from Muğla province of Turkey." , ss.31 - 40, 2007.
ISNAD	TALKIN, Vatan vd. "The biochemical basis of insecticide resistance and determination of esterase enzyme patterns by using page in field collected populations of Drosophila melanogaster from Muğla province of Turkey". (2007), 31-40.

APA	TALKIN V, KÜÇÜKAKYÜZ K, ARSLAN T, ÇÖL B, Göçmen Taşkın B (2007). The biochemical basis of insecticide resistance and determination of esterase enzyme patterns by using page in field collected populations of Drosophila melanogaster from Muğla province of Turkey. Journal of Cell and Molecular Biology, 6(1), 31 - 40.
Chicago	TALKIN Vatan,KÜÇÜKAKYÜZ KÖKSAL,ARSLAN Tülin,ÇÖL Bekir,Göçmen Taşkın Belgin The biochemical basis of insecticide resistance and determination of esterase enzyme patterns by using page in field collected populations of Drosophila melanogaster from Muğla province of Turkey. Journal of Cell and Molecular Biology 6, no.1 (2007): 31 - 40.
MLA	TALKIN Vatan,KÜÇÜKAKYÜZ KÖKSAL,ARSLAN Tülin,ÇÖL Bekir,Göçmen Taşkın Belgin The biochemical basis of insecticide resistance and determination of esterase enzyme patterns by using page in field collected populations of Drosophila melanogaster from Muğla province of Turkey. Journal of Cell and Molecular Biology, vol.6, no.1, 2007, ss.31 - 40.
AMA	TALKIN V,KÜÇÜKAKYÜZ K,ARSLAN T,ÇÖL B,Göçmen Taşkın B The biochemical basis of insecticide resistance and determination of esterase enzyme patterns by using page in field collected populations of Drosophila melanogaster from Muğla province of Turkey. Journal of Cell and Molecular Biology. 2007; 6(1): 31 - 40.
Vancouver	TALKIN V,KÜÇÜKAKYÜZ K,ARSLAN T,ÇÖL B,Göçmen Taşkın B The biochemical basis of insecticide resistance and determination of esterase enzyme patterns by using page in field collected populations of Drosophila melanogaster from Muğla province of Turkey. Journal of Cell and Molecular Biology. 2007; 6(1): 31 - 40.
IEEE	TALKIN V,KÜÇÜKAKYÜZ K,ARSLAN T,ÇÖL B,Göçmen Taşkın B "The biochemical basis of insecticide resistance and determination of esterase enzyme patterns by using page in field collected populations of Drosophila melanogaster from Muğla province of Turkey." Journal of Cell and Molecular Biology, 6, ss.31 - 40, 2007.
ISNAD	TALKIN, Vatan vd. "The biochemical basis of insecticide resistance and determination of esterase enzyme patterns by using page in field collected populations of Drosophila melanogaster from Muğla province of Turkey". Journal of Cell and Molecular Biology 6/1 (2007), 31-40.