Vatan TALKIN
(Muğla Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Muğla, Türkiye)
KÖKSAL KÜÇÜKAKYÜZ
(Muğla Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Muğla, Türkiye)
TÜLİN ARSLAN
(Muğla Üniversitesi, Su Ürünleri Fakültesi, Muğla, Türkiye)
BEKİR ÇÖL
(Muğla Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Muğla, Türkiye)
BELGİN GÖÇMEN TAŞKIN
(Muğla Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Muğla, Türkiye)
Yıl: 2007Cilt: 6Sayı: 1ISSN: 1303-3646 / 1306-0961Sayfa Aralığı: 31 - 40Türkçe

180 3
The biochemical basis of insecticide resistance and determination of esterase enzyme patterns by using page in field collected populations of Drosophila melanogaster from Muğla province of Turkey
Biyokimyasal ve moleküler biyoloji teknikleri, doğal populasyonlarda, potansiyel veya gelişmekte olan direnç mekanizmaları hakkında çok spesifik bilgiler sağlamaktadır. Bu araştırmada, Muğla ve ilçelerinden toplanan Drosophila melanogaster populasyonlarında glutatyon-S-transferaz (GST), yüzde kalan asetilkolin esteraz (AChE) ve genel esteraz aktiviteleri ölçülmüştür. Bununla birlikte poliakrilamid jel elektroforezi (PAGE) yöntemi kullanılarak örneklerdeki esteraz enzim profilleri belirlenmiştir. Yüzde kalan asetilkolin esteraz enzim aktivitesi incelenen örneklerde duyarlı soya göre yüksek bulunmuştur, a ve (3-naftil asetatın substrat olarak kullanılması ile toplam 21 esteraz bandı elde edilmiştir, bu bantların 18 tanesi a esteraz, iki tanesi (3 esteraz ve bir tanesi ise a/B esteraz olarak sınıflandırılmıştır.
Fen > Tıp > Biyokimya ve Moleküler Biyoloji
DergiAraştırma MakalesiErişime Açık
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