Visceral Leishmaniasis’li Köpeklerde Arjinin, Simetrik ve Asimetrik Dimetilarjinin Düzeylerinin Araştırılması

GÜLTEKİN, Mehmet; PAŞA, Serdar; Ural, Kerem; ERDOĞAN, HASAN

Visceral Leishmaniasis’li Köpeklerde Arjinin, Simetrik ve Asimetrik Dimetilarjinin Düzeylerinin Araştırılması

Yürütücü

Mehmet GÜLTEKİN (Adnan Menderes Üniversitesi, Veteriner Fakültesi, İç Hastalıkları Anabilim Dalı, Aydın, Türkiye)

Araştırmacı(lar)

Serdar PAŞA, (Adres Yazılmamış)

Kerem URAL, (Adres Yazılmamış)

Hasan ERDOĞAN (Adres Yazılmamış)

14 9

Proje Grubu: TOVAG Sayfa Sayısı: 41 Proje No: 117O894 Proje Bitiş Tarihi: 15.04.2019 Metin Dili: Türkçe İndeks Tarihi: 09-03-2020

Visceral Leishmaniasis’li Köpeklerde Arjinin, Simetrik ve Asimetrik Dimetilarjinin Düzeylerinin Araştırılması

Öz:

Bu çalışmada Visceral Leishmaniasis'li (VL), hastalığın farklı evrelerindeki köpeklerde arjinin, simetrik dimetilarjinin (SDMA) ve asimetrik dimetilarjinin (ADMA) konsantrasyonlarının ortaya konulması amaçlandı. VL, köpeklerde kum sinekleri tarafından L. infantum'un nakledilmesi ile oluşan, zoonoz nitelikte, kronik, sistemik protozoal bir hastalıktır. Etken makrofajlarda çoğalarak kardiyovasküler ve renal sistem dahil olmak üzere çok yönlü klinik bulgulara neden olabilmektedir. SDMA ve ADMA molekülleri arjinin metilasyonu sonucunda açığa çıkmakta, renal ve kardiyovasküler hastalıkların tanı ve prognozunda önemli biyobelirteçler olarak görülmektedir. Çalışmada 20 sağlıklı, 40 VL'li olmak üzere her iki cinsiyetten, farklı yaş ve orta büyüklükteki ırklardan toplam 60 (n=60) köpek kullanıldı. VL'li köpekler (n=40) klinik ve laboratuvar bulgular temelinde Evre I (n=12), Evre II (n=14), Evre III (n=7) ve Evre IV (n=7) olarak ayrıca gruplandırıldı. Köpeklerden tek sefer yapılan örnekleme ile rutin hemato-biyokimyasal parametreler (WBC, RBC, HCT, MCV, MCH, PLT, total Protein, albumin, ALT, AST), renal değerlendirme amacıyla plazma üre, kreatinin, sistatin C, fosfor, potasyum konsantrasyonları ile idrar protein/kreatinin oranı, kardiyovasküler değerlendirme amacıyla plazma Troponin I, Miyoglobin, CK-MB, kolestrol, HDL, LDL, nitrik oksit (NO) ve endotelyal nitrik oksit sentaz (eNOS) düzeyleri ile sistemik arteriyel kan basıncı ölçüldü. Plazma arjinin, SDMA ve ADMA düzeyleri HPLC yöntemi ile belirlendi. Sağlıklı grubun verileri VL'li köpekler ve evrelere göre gruplandırılmış VL'li köpeklerin verileri ile karşılaştırıldı. Sağlıklı köpeklere göre VL'li köpeklerde sistolik ve diastolik kan basıncı, kreatinin, sistatin-C, fosfor, potasyum ve LDL konsantrasyonları ile idrar protein/kreatinin oranı, NO miktarı ve CK- MB aktivitesi yüksek; RBC ve PLT sayıları ile HCT, MCV ve MCHC değerleri, HDL konsantrasyonu ise düşük bulundu. VL'li köpeklerde arjinin konsantrasyonu düşük, ADMA ve SDMA düzeyleri ise yüksek bulundu. Evrelere göre gruplandırılan köpeklerde arjinin konsantrasyonundaki farklılıkların istatistiksel anlamlı olmadığı görüldü. Plazma ADMA konsantrasyonu sağlıklı gruba göre tüm evrelerdeki köpeklerde yüksek bulundu. Plazma SDMA konsantrasyonu Evre IV grubu köpeklerde sağlıklı ve Evre III gruplarına göre yüksek belirlendi. Sonuç olarak, VL'li köpeklerde arjinin konsantrasyonunda azalma ve ADMA konsantrasyonundaki artış ilk defa gösterildi. VL'li köpeklerde SDMA konsantrasyonunda belirlenen artışın önceki çalışmalarla uyumlu olduğu görüldü. Elde edilen bulguların VL'nin renal ve kardiyovaskuler patofizyolojisinin açıklanmasına yönelik ileride gerçekleştirilecek tanısal ve terapotik çalışmalara kaynak oluşturabileceği düşünüldü. VL'de belirlenen değişikliklerin detaylandırılmasına yönelik hasta takibi ve sağaltımı kapsayan daha fazla klinik çalışma yapılmasının fayda sağlayabileceği kanısına varıldı.

Anahtar Kelime: nitrik oksit adma sdma arjinin köpek visceral leishmaniasis

Konular: Veterinerlik

Erişim Türü: Erişime Açık

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APA	GÜLTEKİN M, PAŞA S, Ural K, ERDOĞAN H (2019). Visceral Leishmaniasis’li Köpeklerde Arjinin, Simetrik ve Asimetrik Dimetilarjinin Düzeylerinin Araştırılması. , 1 - 41.
Chicago	GÜLTEKİN Mehmet,PAŞA Serdar,Ural Kerem,ERDOĞAN HASAN Visceral Leishmaniasis’li Köpeklerde Arjinin, Simetrik ve Asimetrik Dimetilarjinin Düzeylerinin Araştırılması. (2019): 1 - 41.
MLA	GÜLTEKİN Mehmet,PAŞA Serdar,Ural Kerem,ERDOĞAN HASAN Visceral Leishmaniasis’li Köpeklerde Arjinin, Simetrik ve Asimetrik Dimetilarjinin Düzeylerinin Araştırılması. , 2019, ss.1 - 41.
AMA	GÜLTEKİN M,PAŞA S,Ural K,ERDOĞAN H Visceral Leishmaniasis’li Köpeklerde Arjinin, Simetrik ve Asimetrik Dimetilarjinin Düzeylerinin Araştırılması. . 2019; 1 - 41.
Vancouver	GÜLTEKİN M,PAŞA S,Ural K,ERDOĞAN H Visceral Leishmaniasis’li Köpeklerde Arjinin, Simetrik ve Asimetrik Dimetilarjinin Düzeylerinin Araştırılması. . 2019; 1 - 41.
IEEE	GÜLTEKİN M,PAŞA S,Ural K,ERDOĞAN H "Visceral Leishmaniasis’li Köpeklerde Arjinin, Simetrik ve Asimetrik Dimetilarjinin Düzeylerinin Araştırılması." , ss.1 - 41, 2019.
ISNAD	GÜLTEKİN, Mehmet vd. "Visceral Leishmaniasis’li Köpeklerde Arjinin, Simetrik ve Asimetrik Dimetilarjinin Düzeylerinin Araştırılması". (2019), 1-41.

APA	GÜLTEKİN M, PAŞA S, Ural K, ERDOĞAN H (2019). Visceral Leishmaniasis’li Köpeklerde Arjinin, Simetrik ve Asimetrik Dimetilarjinin Düzeylerinin Araştırılması. , 1 - 41.
Chicago	GÜLTEKİN Mehmet,PAŞA Serdar,Ural Kerem,ERDOĞAN HASAN Visceral Leishmaniasis’li Köpeklerde Arjinin, Simetrik ve Asimetrik Dimetilarjinin Düzeylerinin Araştırılması. (2019): 1 - 41.
MLA	GÜLTEKİN Mehmet,PAŞA Serdar,Ural Kerem,ERDOĞAN HASAN Visceral Leishmaniasis’li Köpeklerde Arjinin, Simetrik ve Asimetrik Dimetilarjinin Düzeylerinin Araştırılması. , 2019, ss.1 - 41.
AMA	GÜLTEKİN M,PAŞA S,Ural K,ERDOĞAN H Visceral Leishmaniasis’li Köpeklerde Arjinin, Simetrik ve Asimetrik Dimetilarjinin Düzeylerinin Araştırılması. . 2019; 1 - 41.
Vancouver	GÜLTEKİN M,PAŞA S,Ural K,ERDOĞAN H Visceral Leishmaniasis’li Köpeklerde Arjinin, Simetrik ve Asimetrik Dimetilarjinin Düzeylerinin Araştırılması. . 2019; 1 - 41.
IEEE	GÜLTEKİN M,PAŞA S,Ural K,ERDOĞAN H "Visceral Leishmaniasis’li Köpeklerde Arjinin, Simetrik ve Asimetrik Dimetilarjinin Düzeylerinin Araştırılması." , ss.1 - 41, 2019.
ISNAD	GÜLTEKİN, Mehmet vd. "Visceral Leishmaniasis’li Köpeklerde Arjinin, Simetrik ve Asimetrik Dimetilarjinin Düzeylerinin Araştırılması". (2019), 1-41.