MCF-7 Meme Kanser Hücre Hattında Warburg Etkisinin ve Hipoksinin Glikoliz Enzimleri Gen Ekspresyonu Düzeyinde Araştırılması

BİLDİK, Ayşehül; KIRAL, Funda Kargın; EKREN ASICI, GAMZE SEVRI

MCF-7 Meme Kanser Hücre Hattında Warburg Etkisinin ve Hipoksinin Glikoliz Enzimleri Gen Ekspresyonu Düzeyinde Araştırılması

Yürütücü

Ayşehül BİLDİK (AYDIN ADNAN MENDERES ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ, BİYOKİMYA)

Araştırmacı(lar)

Funda Kargın KIRAL, (AYDIN ADNAN MENDERES ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ, BİYOKİMYA)

Gamze Sevri EKREN AŞICI (AYDIN ADNAN MENDERES ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ, BİYOKİMYA)

11 7

Proje Grubu: KBAG Sayfa Sayısı: 102 Proje No: 117Z735 Proje Bitiş Tarihi: 15.04.2019 Metin Dili: Türkçe İndeks Tarihi: 08-11-2020

MCF-7 Meme Kanser Hücre Hattında Warburg Etkisinin ve Hipoksinin Glikoliz Enzimleri Gen Ekspresyonu Düzeyinde Araştırılması

Öz:

Projede, MCF-7 meme kanser hücrelerinde, glukoz ve oksijen yokluğunda Warburg fenomeni ve Crabtree etkisinin glikolizin metabolik kontrol enzimlerinin gen ekspresyonu düzeyinde araştırılması amaçlandı. Kanser hücreleri proliferasyon üzerine kurulmuş normal hücrelerden farklı bir metabolizma gösterir. Normal hücrelere göre, kandan glukozu 5-10 kat daha fazla aldığı, oksijen varlığından bağımsız aerobik şartlar altında laktik asit üreten glikolitik yolu tercih ettiği ileri sürülmektedir. Kanser hücre metabolizması ile normal hücre metabolizması arasındaki bu fark Warburg fenomeni olarak adlandırılır. Metabolik enzimler, kanser hücresinin büyümesinde önemli düzenleyicidir. Hipoksi, kanser gelişiminde çok yönlü rolüyle uzun zamandır bilinmektedir ve katı tümörlerin en önemli özelliklerinden biri olarak kabul edilmektedir. Dolayısı ile kanser hücresinde karbonhidrat metabolizmasının iki temel özelliği hipoksi ve hipoglisemidir. Planlanan çalışmada kanser hücresinin iki temel özelliği birlikte değerlendirildi. Bu amaçla, MCF-7 hücreleri, 0; 5,5; 15 ve 55 mM glikoz konsantrasyonları içeren mediumlarda 3, 6, 12 ve 24 saat normoksi (% 20 O2) ve hipoksi (%1O2) koşullarına maruz bırakıldı. Uygulama koşullarının hücre proliferasyonu üzerine etkileri WST-1 canlılık testi ile belirlendi. Deneme sonunda hücrelerden izole RNA?larda, hekzokinaz 2, piruvat kinaz M2, glukoz 6 fosfat dehidrogenaz, laktat dehidrogenaz A, fosfofruktokinaz1, gliseraldehit 3 fosfat dehidrogenaz enzimlerinin gen ekspresyonları ile HIF? ve GLUT1 genlerinin ekspresyon düzeyleri RT-PCR?da Syber green yöntemi ile kantitatif olarak belirlendi. Hücrelerin mikro çevresindeki glukoz ve laktik asit miktarları kolorimetrik olarak tayin edildi. Çalışmanın laktat sonuçları, MCF 7 hücrelerinde Crabtree etkisinin baskın olduğunu gösterdi. Glikoliz enzimleri ve G6PD enziminin mRNA ifadeleri değerlendirildiğinde, aerobik glikoliz akışının aktif olduğu; kanser hücresinin çoğalması sırasında ihtiyaç duyulan biyomoleküllerin sentezi için gerekli olan pentoz fosfat yolunun, yüksek glukoz ve hipoksik ortamda aerobik glikolizden daha etkin rol oynadığı sonucuna varıldı.

Anahtar Kelime: gen ekspresyonu aerobik glikoliz glut1 hıf? hipoksi anahtar kelimeler: mcf7

Erişim Türü: Erişime Açık

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APA	BİLDİK A, KIRAL F, EKREN ASICI G (2019). MCF-7 Meme Kanser Hücre Hattında Warburg Etkisinin ve Hipoksinin Glikoliz Enzimleri Gen Ekspresyonu Düzeyinde Araştırılması. , 1 - 102.
Chicago	BİLDİK Ayşehül,KIRAL Funda Kargın,EKREN ASICI GAMZE SEVRI MCF-7 Meme Kanser Hücre Hattında Warburg Etkisinin ve Hipoksinin Glikoliz Enzimleri Gen Ekspresyonu Düzeyinde Araştırılması. (2019): 1 - 102.
MLA	BİLDİK Ayşehül,KIRAL Funda Kargın,EKREN ASICI GAMZE SEVRI MCF-7 Meme Kanser Hücre Hattında Warburg Etkisinin ve Hipoksinin Glikoliz Enzimleri Gen Ekspresyonu Düzeyinde Araştırılması. , 2019, ss.1 - 102.
AMA	BİLDİK A,KIRAL F,EKREN ASICI G MCF-7 Meme Kanser Hücre Hattında Warburg Etkisinin ve Hipoksinin Glikoliz Enzimleri Gen Ekspresyonu Düzeyinde Araştırılması. . 2019; 1 - 102.
Vancouver	BİLDİK A,KIRAL F,EKREN ASICI G MCF-7 Meme Kanser Hücre Hattında Warburg Etkisinin ve Hipoksinin Glikoliz Enzimleri Gen Ekspresyonu Düzeyinde Araştırılması. . 2019; 1 - 102.
IEEE	BİLDİK A,KIRAL F,EKREN ASICI G "MCF-7 Meme Kanser Hücre Hattında Warburg Etkisinin ve Hipoksinin Glikoliz Enzimleri Gen Ekspresyonu Düzeyinde Araştırılması." , ss.1 - 102, 2019.
ISNAD	BİLDİK, Ayşehül vd. "MCF-7 Meme Kanser Hücre Hattında Warburg Etkisinin ve Hipoksinin Glikoliz Enzimleri Gen Ekspresyonu Düzeyinde Araştırılması". (2019), 1-102.

APA	BİLDİK A, KIRAL F, EKREN ASICI G (2019). MCF-7 Meme Kanser Hücre Hattında Warburg Etkisinin ve Hipoksinin Glikoliz Enzimleri Gen Ekspresyonu Düzeyinde Araştırılması. , 1 - 102.
Chicago	BİLDİK Ayşehül,KIRAL Funda Kargın,EKREN ASICI GAMZE SEVRI MCF-7 Meme Kanser Hücre Hattında Warburg Etkisinin ve Hipoksinin Glikoliz Enzimleri Gen Ekspresyonu Düzeyinde Araştırılması. (2019): 1 - 102.
MLA	BİLDİK Ayşehül,KIRAL Funda Kargın,EKREN ASICI GAMZE SEVRI MCF-7 Meme Kanser Hücre Hattında Warburg Etkisinin ve Hipoksinin Glikoliz Enzimleri Gen Ekspresyonu Düzeyinde Araştırılması. , 2019, ss.1 - 102.
AMA	BİLDİK A,KIRAL F,EKREN ASICI G MCF-7 Meme Kanser Hücre Hattında Warburg Etkisinin ve Hipoksinin Glikoliz Enzimleri Gen Ekspresyonu Düzeyinde Araştırılması. . 2019; 1 - 102.
Vancouver	BİLDİK A,KIRAL F,EKREN ASICI G MCF-7 Meme Kanser Hücre Hattında Warburg Etkisinin ve Hipoksinin Glikoliz Enzimleri Gen Ekspresyonu Düzeyinde Araştırılması. . 2019; 1 - 102.
IEEE	BİLDİK A,KIRAL F,EKREN ASICI G "MCF-7 Meme Kanser Hücre Hattında Warburg Etkisinin ve Hipoksinin Glikoliz Enzimleri Gen Ekspresyonu Düzeyinde Araştırılması." , ss.1 - 102, 2019.
ISNAD	BİLDİK, Ayşehül vd. "MCF-7 Meme Kanser Hücre Hattında Warburg Etkisinin ve Hipoksinin Glikoliz Enzimleri Gen Ekspresyonu Düzeyinde Araştırılması". (2019), 1-102.