The relationship between carnitine levels and lipid peroxidation in glial brain tumors

KAYMAZİ, Memduh; PAŞAOĞLU, Aydın; PAŞAOĞLU, Hatice; BİBEROĞLU, Gürsel; BAYRAKTAR, NILÜFER; KULAKSIZOĞLU, Sevsen

The relationship between carnitine levels and lipid peroxidation in glial brain tumors

Nilüfer BAYRAKTAR, (Ankara Eğitim ve Araştırma Hastanesi, Biyokimya Laboratuvarı, Ankara, Türkiye)

Hatice PAŞAOĞLU, (Gazi Üniversitesi, Tıp Fakültesi, Tıbbi Biyokimya Anabilim Dalı, Ankara, Türkiye)

Aydın PAŞAOĞLU, (Gazi Üniversitesi, Tıp Fakültesi, Nöroşirürji Anabilim Dalı, Ankara, Türkiye)

Memduh KAYMAZİ, (Gazi Üniversitesi, Tıp Fakültesi, Nöroşirürji Anabilim Dalı, Ankara, Türkiye)

Gürsel BİBEROĞLU, (Gazi Üniversitesi, Tıp Fakültesi, Pediatrik Metabolizma Hastalıkları Anabilim Dalı, Ankara, Türkiye)

Sevsen KULAKSIZOĞLU (Başkent Üniversitesi, Konya Araştırma ve Uygulama Merkezi, Adana, Türkiye)

Turkish Journal of Medical Sciences

4 0

Yıl: 2008 Cilt: 38 Sayı: 4 Sayfa Aralığı: 293 - 299 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

The relationship between carnitine levels and lipid peroxidation in glial brain tumors

Öz:

Amaç: Serbest oksijen radikallerinin ve lipid peroksidasyonunun hücre zarı hasarına neden olduğu ve onkogenezde rol oynadığı düşünülmektedir. Hücre içi kısa, orta ve uzun zincirli yağ asidi metabolizmasında rol oynayan karnitin ve açil esterleri, serbest radikal toksisitesine karşı savunma mekanizmalarından birisidir. Karnitinin koruyucu etkilerinin lipid metabolizması üzerindeki rolüyle ilişkili olduğu düşünülmektedir. Çalışmamızda, karsinogenezisde karnitin-açilkarnitinlerin lipid peroksidasyonuyla arasında bir ilişki olup olmadığını açıklamak için glial tümörlerdeki (glioblastoma multiforme (GBM) n = 29, yüksek grade astrositoma n = 8 and düşük grade astrositoma n = 8) MDA ve serbest karnitin, C2, C3, C4, C5, C6, C8, C10, C12, C14:1, C14, C16:1, C16, C18:1, C18, C20:4 karnitin düzeyleri arasındaki ilişkiyi inceledik. Yöntem ve Gereç: Glial tümörlerdeki serbest karnitin ve C2, C3, C4, C5, C6, C8, C10, C12, C14:1, C14, C16:1, C16, C18:1, C18, C20:4 karnitin düzeyleri ardışık kütle spektrometrisinde çalışıldı. MDA düzeyleri ise HPLC sistemi kullanılarak ölçüldü. Bulgular: GBM’ de MDA ve C20:4 karnitin düzeyleri arasında anlamlı bir korelasyon bulundu. MDA düzeyleri artarken C20:4 karnitin düzeylerinin de arttığı görüldü (p = 0.000, r = 0.916). Fakat diğer gruplar arasında anlamlı bir korelasyon görülmedi. Sonuç: Sonuç olarak sadece MDA-karnitin ilişkisinin ölçümü anlamlı bir korelasyon yansıtmamaktadır. Antioksidan yapılar ve tümör hücre metabolizmasını gösteren parametreleri de içeren daha kapsamlı çalışmalarla konunun incelenmesi gerekmektedir

Anahtar Kelime: Kromatografi, yüksek basınçlı sıvı Karnitin Gliom serbest oksijen radikalleri Tandem mass spektrometri Beyin neoplazmları Lipid peroksidasyonu

Konular: Cerrahi

Glial beyin tümörlerinde karnitin düzeyleri ve lipid peroksidasyonu arasındaki ilişki

Öz:

Aim: It is assumed that reactive oxygen species and lipid peroxidation cause cell membrane damage and play a role in oncogenesis. Carnitine and acyl esters, which play a role in intracellular short-, medium-, and long-chain fatty acid metabolism, are one of the defense mechanisms against free radical toxicity. It is thought that the protective effects of carnitine are related with its role in lipid metabolism. In our study, we analyzed the relation between malondialdehyde (MDA) and free carnitine, and C2, C3, C4, C5, C6, C8, C10, C12, C14:1, C14, C16:1, C16, C18:1, C18, C20:4 carnitine levels in glial tumors [glioblastoma multiforme (GBM) (n = 29), high-grade astrocytoma (n = 8) and low-grade astrocytoma (n = 8)] to determine whether there is a relation between carnitine-acyl carnitines and lipid peroxidation in carcinogenesis. Materials and Methods: The present study examined the free carnitine and C2, C3, C4, C5, C6, C8, C10, C12, C14:1, C14, C16:1, C16, C18:1, C18, C20:4 levels of glial tumors in tandem mass spectrometry. We measured MDA levels using HPLC system. Results: There was a significant correlation between MDA and C20:4 carnitine levels in GBM. C20:4 carnitine levels increased with increasing MDA levels (p = 0.000, r = 0.916), but no significant correlation was found in the other groups. Conclusions: In conclusion, measurement of only carnitine-MDA relation did not reflect any significant correlation. Detailed studies, including measurement of parameters showing antioxidant status and tumor cellular metabolism, are necessary.

Anahtar Kelime: Tandem Mass Spectrometry Brain Neoplasms Lipid Peroxidation Chromatography, High Pressure Liquid Carnitine Glioma free oxygen radicals

Konular: Cerrahi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	BAYRAKTAR N, PAŞAOĞLU H, PAŞAOĞLU A, KAYMAZİ M, BİBEROĞLU G, KULAKSIZOĞLU S (2008). The relationship between carnitine levels and lipid peroxidation in glial brain tumors. , 293 - 299.
Chicago	BAYRAKTAR NILÜFER,PAŞAOĞLU Hatice,PAŞAOĞLU Aydın,KAYMAZİ Memduh,BİBEROĞLU Gürsel,KULAKSIZOĞLU Sevsen The relationship between carnitine levels and lipid peroxidation in glial brain tumors. (2008): 293 - 299.
MLA	BAYRAKTAR NILÜFER,PAŞAOĞLU Hatice,PAŞAOĞLU Aydın,KAYMAZİ Memduh,BİBEROĞLU Gürsel,KULAKSIZOĞLU Sevsen The relationship between carnitine levels and lipid peroxidation in glial brain tumors. , 2008, ss.293 - 299.
AMA	BAYRAKTAR N,PAŞAOĞLU H,PAŞAOĞLU A,KAYMAZİ M,BİBEROĞLU G,KULAKSIZOĞLU S The relationship between carnitine levels and lipid peroxidation in glial brain tumors. . 2008; 293 - 299.
Vancouver	BAYRAKTAR N,PAŞAOĞLU H,PAŞAOĞLU A,KAYMAZİ M,BİBEROĞLU G,KULAKSIZOĞLU S The relationship between carnitine levels and lipid peroxidation in glial brain tumors. . 2008; 293 - 299.
IEEE	BAYRAKTAR N,PAŞAOĞLU H,PAŞAOĞLU A,KAYMAZİ M,BİBEROĞLU G,KULAKSIZOĞLU S "The relationship between carnitine levels and lipid peroxidation in glial brain tumors." , ss.293 - 299, 2008.
ISNAD	BAYRAKTAR, NILÜFER vd. "The relationship between carnitine levels and lipid peroxidation in glial brain tumors". (2008), 293-299.

APA	BAYRAKTAR N, PAŞAOĞLU H, PAŞAOĞLU A, KAYMAZİ M, BİBEROĞLU G, KULAKSIZOĞLU S (2008). The relationship between carnitine levels and lipid peroxidation in glial brain tumors. Turkish Journal of Medical Sciences, 38(4), 293 - 299.
Chicago	BAYRAKTAR NILÜFER,PAŞAOĞLU Hatice,PAŞAOĞLU Aydın,KAYMAZİ Memduh,BİBEROĞLU Gürsel,KULAKSIZOĞLU Sevsen The relationship between carnitine levels and lipid peroxidation in glial brain tumors. Turkish Journal of Medical Sciences 38, no.4 (2008): 293 - 299.
MLA	BAYRAKTAR NILÜFER,PAŞAOĞLU Hatice,PAŞAOĞLU Aydın,KAYMAZİ Memduh,BİBEROĞLU Gürsel,KULAKSIZOĞLU Sevsen The relationship between carnitine levels and lipid peroxidation in glial brain tumors. Turkish Journal of Medical Sciences, vol.38, no.4, 2008, ss.293 - 299.
AMA	BAYRAKTAR N,PAŞAOĞLU H,PAŞAOĞLU A,KAYMAZİ M,BİBEROĞLU G,KULAKSIZOĞLU S The relationship between carnitine levels and lipid peroxidation in glial brain tumors. Turkish Journal of Medical Sciences. 2008; 38(4): 293 - 299.
Vancouver	BAYRAKTAR N,PAŞAOĞLU H,PAŞAOĞLU A,KAYMAZİ M,BİBEROĞLU G,KULAKSIZOĞLU S The relationship between carnitine levels and lipid peroxidation in glial brain tumors. Turkish Journal of Medical Sciences. 2008; 38(4): 293 - 299.
IEEE	BAYRAKTAR N,PAŞAOĞLU H,PAŞAOĞLU A,KAYMAZİ M,BİBEROĞLU G,KULAKSIZOĞLU S "The relationship between carnitine levels and lipid peroxidation in glial brain tumors." Turkish Journal of Medical Sciences, 38, ss.293 - 299, 2008.
ISNAD	BAYRAKTAR, NILÜFER vd. "The relationship between carnitine levels and lipid peroxidation in glial brain tumors". Turkish Journal of Medical Sciences 38/4 (2008), 293-299.