Consequences of social ısolation in rats on their antioxidant defense system and erythrocyte deformability

Aşçıoğlu, Meral; AYDOĞAN, Sami; ÇOMU, Faruk Metin

Consequences of social ısolation in rats on their antioxidant defense system and erythrocyte deformability

Sami AYDOĞAN, (Erciyes Üniversitesi, Tıp Fakültesi, Fizyoloji Anabilim Dalı, Kayseri, Türkiye)

Meral AŞÇIOĞLU, (Erciyes Üniversitesi, Tıp Fakültesi, Fizyoloji Anabilim Dalı, Kayseri, Türkiye)

Faruk Metin ÇOMU (Kırıkkale Üniversitesi, Tıp Fakültesi, Fizyoloji Anabilim Dalı, Kırıkkale, Türkiye)

Erciyes Tıp Dergisi

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Yıl: 2009 Cilt: 31 Sayı: 1 Sayfa Aralığı: 9 - 14 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Consequences of social ısolation in rats on their antioxidant defense system and erythrocyte deformability

Öz:

Amaç: Bu çalışmada amacımız, eritrositlerin biyokimyasal ve mekanik özelliklerinin dolaşımın etkinliğinin belirlenmesindeki önemini dikkate alarak, sosyal izolasyon uygulanan sıçanlarda oluşan oksidatif stresin eritrositlerin deformabilite özellikleri ve antioksidan sistem üzerine etkilerini incelemektir. Gereç ve Yöntemler: Çalışmada 40 adet Sprague Dawley sıçan, 2 aylık (genç) ve 12 aylık (erişkin) olmak üzere iki gruba ayrıldı. Daha sonra her gruptan 10'ar sıçan, 12/12 saat aydınlık/karanlık siklusunda, 21 gün boyunca tek başlarına ayrı kafeslere alınarak, sosyal izolasyon stresi yaratıldı. 21. gün sonunda alınan kan örneklerinde, eritrositlerde antioksidan enzimlerden SOD, CAT ve lipid peroksidasyonun bir göstergesi olarak MDA düzeyleri spektrofotometrik olarak ölçüldü. Plazma nitrik oksit değişiklikleri total nitrit-nitrat ölçümüne dayalı Griess yöntemi ile tayin edildi. Ayrıca hazırlanan eritrosit süspansiyonları %5 hematokrite ayarlanarak, filtrasyon yöntemi ile, relatif filtrasyon hızı (RFR), relatif filtrasyon zamanı (RFT) ve relatif direnç (Rrel) değerleri ölçüldü.Bulgular: SOD ve CAT düzeyleri hem genç hemde erişkin olan sosyal olarak izole edilmiş sıçanlarda istatistiksel açıdan anlamlı derecede düşük bulunmuştur. Sosyal izolasyon uygulanan sıçanlarda ,nitrik oksit düzeyleri de erişkinlerde daha belirgin olmak üzere hem genç hem de erişkinlerde daha düşüktür.Lipid peroksidasyon ürünü MDA düzeyleri de her iki grupta kontrollere göre azalmıştır.Eritrosit deformabilite indekslerinden RFR değerleri izole sıçanlarda önemli derecede yüksek bulunurken, RFT hem genç hem de erişkinlerde kontrollere göre düşük bulunmuştur.Ancak eritrositlerin Rrel değerlerinde önemli derecede bir değişiklik olmamıştır.Sonuç: Verilerimiz sosyal izolasyonun sıçanlarda plazma nitrik oksit düzeylerini azalttığı ve muhtemelen bu azalmayla bağlantılı olarak eritrosit membranında lipid peroksidasyonun da azaldığına işaret etmektedir. Ayrıca, sosyal izolasyon stresi antioksidan enzim aktivitelerini de değiştirmiş ve buda muhtemelen daha uzun süreli ve tekrarlanan yalnızlık durumlarında, özellikle yaşamın ilk dönemlerinde doku perfüzyonu problemlerine yol açabilecek deformabilite değişiklikleri ile sonuçlanabilecektir.

Anahtar Kelime: Nitrik oksit Sıçanlar Oksidatif stres Spektrofotometri Antioksidanlar Akyuvar biçim değişimi Modeller, hayvan Sıçan, Sprague-Dawley Sosyal izolasyon

Konular: Genel ve Dahili Tıp

Sıçanlarda sosyal izolasyonun oksidan stres ve eritrosit deformabilitesi üzerine etkileri

Öz:

Purpose: In this study, our purpose was to investigate effect of oxidative stress composing in isolated rats on erythrocyte deformability and antioxidant system, considering importance of mechanical, and biochemical properties of erythrocytes in efficiency of blood circulation Material and Methods: Fourty Sprague-Dawley rats were divided into two groups according to their ages; where one of them consisted of 2 months old rats and the other 12 months old rats. Then, randomly selected ten rats in each group were isolated separately in a cage and hold 12/12 hour light-dark cycle lasting 21 days. Blood samples were collected at the end of the 21st day, and superoxide dismutase (SOD), catalase (CAT) activities and malondialdehyde (MDA) levels were measured spectrophotometrically. Changes in plasma nitric oxide level were determined by Griess method depending on total nitrite-nitrate. In addition, relative filtration rate (RFR), relative filtration time (RFT) and relative resistance (Rrel) of the erythrocytes were calculated as the indexes of erythrocyte deformability.Results: The SOD and CAT levels were found significantly lower in both of young and adult isolated rats when compared to the young and adult control groups. The nitric oxide levels were also found significantly lower in the young and adult isolated rats when compared to their control groups. The peroxidation of the lipids were also decreased by social isolation, in the erythrocyte membrane of both young and adult isolated rats compared to the controls. The indexes of erythrocyte deformability, RFR was found significantly higher, whereas the RFT was significantly lower in young and adult isolated rats compared to the young and adult controls. However, the Rrel of the erythrocytes were not altered dramatically.Conclusion: As a consequence, our data reveals that the social isolation causes a lack of plasma nitric oxide levels in the socially isolated rats and probably due to this decrease in one of the major oxidants for the erythrocyte membrane results in the decreased lipid peroxidation. Furthermore, our results reveal that the social isolation stress causes alterations in the antioxidant defense system and these alteration results in the changes in erythrocyte deformability reflecting that some tissue perfusion problems can occur with long term and repeated loneliness and especially in the early stages of the life span.

Anahtar Kelime: Erythrocyte Deformability Models, Animal Rats, Sprague-Dawley Social Isolation Nitric Oxide Rats Oxidative Stress Spectrophotometry Antioxidants

Konular: Genel ve Dahili Tıp

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	AYDOĞAN S, Aşçıoğlu M, ÇOMU F (2009). Consequences of social ısolation in rats on their antioxidant defense system and erythrocyte deformability. , 9 - 14.
Chicago	AYDOĞAN Sami,Aşçıoğlu Meral,ÇOMU Faruk Metin Consequences of social ısolation in rats on their antioxidant defense system and erythrocyte deformability. (2009): 9 - 14.
MLA	AYDOĞAN Sami,Aşçıoğlu Meral,ÇOMU Faruk Metin Consequences of social ısolation in rats on their antioxidant defense system and erythrocyte deformability. , 2009, ss.9 - 14.
AMA	AYDOĞAN S,Aşçıoğlu M,ÇOMU F Consequences of social ısolation in rats on their antioxidant defense system and erythrocyte deformability. . 2009; 9 - 14.
Vancouver	AYDOĞAN S,Aşçıoğlu M,ÇOMU F Consequences of social ısolation in rats on their antioxidant defense system and erythrocyte deformability. . 2009; 9 - 14.
IEEE	AYDOĞAN S,Aşçıoğlu M,ÇOMU F "Consequences of social ısolation in rats on their antioxidant defense system and erythrocyte deformability." , ss.9 - 14, 2009.
ISNAD	AYDOĞAN, Sami vd. "Consequences of social ısolation in rats on their antioxidant defense system and erythrocyte deformability". (2009), 9-14.

APA	AYDOĞAN S, Aşçıoğlu M, ÇOMU F (2009). Consequences of social ısolation in rats on their antioxidant defense system and erythrocyte deformability. Erciyes Tıp Dergisi, 31(1), 9 - 14.
Chicago	AYDOĞAN Sami,Aşçıoğlu Meral,ÇOMU Faruk Metin Consequences of social ısolation in rats on their antioxidant defense system and erythrocyte deformability. Erciyes Tıp Dergisi 31, no.1 (2009): 9 - 14.
MLA	AYDOĞAN Sami,Aşçıoğlu Meral,ÇOMU Faruk Metin Consequences of social ısolation in rats on their antioxidant defense system and erythrocyte deformability. Erciyes Tıp Dergisi, vol.31, no.1, 2009, ss.9 - 14.
AMA	AYDOĞAN S,Aşçıoğlu M,ÇOMU F Consequences of social ısolation in rats on their antioxidant defense system and erythrocyte deformability. Erciyes Tıp Dergisi. 2009; 31(1): 9 - 14.
Vancouver	AYDOĞAN S,Aşçıoğlu M,ÇOMU F Consequences of social ısolation in rats on their antioxidant defense system and erythrocyte deformability. Erciyes Tıp Dergisi. 2009; 31(1): 9 - 14.
IEEE	AYDOĞAN S,Aşçıoğlu M,ÇOMU F "Consequences of social ısolation in rats on their antioxidant defense system and erythrocyte deformability." Erciyes Tıp Dergisi, 31, ss.9 - 14, 2009.
ISNAD	AYDOĞAN, Sami vd. "Consequences of social ısolation in rats on their antioxidant defense system and erythrocyte deformability". Erciyes Tıp Dergisi 31/1 (2009), 9-14.