Yıl: 2009 Cilt: 33 Sayı: 2 Sayfa Aralığı: 89 - 100 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Comparison of GST ısoenzyme expression in normal and neoplastic breast tissue: correlation with clinical and prognostic factors

Öz:
Meme dokusunda bulunan Glutatyon-S-transferazlar, meme tümörlerinin kemoterapiye verdiği cevapta ve karsinojenlerin mutajenik etkilerine duyarlılıkta önemlidir. Bu çalışmada, 43 invasif duktal kanser ve normal meme dokusunda glutatyon-S-transferaz enzimlerinin (alfa, mü, pi ve teta) immünohistokimyasal boyanma özellikleri araştırılmıştır. Ayrıca, GST izozimlerinin salınımları ve hastaların klinik özellikleri arasındaki ilişkiler de tespit edilmiştir. GST alfa, mü, pi ve teta izozimleri, vakaların % 100’ünde normal ve tümörlü meme dokularında farklı boyanma şiddetinde ve yaygın sitoplazmik olarak tespit edildi. GST alfa, mü ve pi enzimleri normal epitelde invasif tümör dokularına göre daha şiddetli boyanma göstermiştir (P < 0,05). Ancak, istatistiksel olarak normal ve tümör epitel hücrelerinde GST teta salınımlarında bir fark tespit edilmemiştir (P > 0,05). Bu çalışmada, mikrokalsifikasyon durumu ve GST mü; menapoz durumu ve GST alfa; tumor evresi ve GST mü salınımları arasında önemli bir ilişki gözlenmiştir (P < 0,05). GST izozimleri ile estrojen reseptör durumu, evre, sigara içimi, kemoterapi, çocuk sayısı, yaş ve hormon terapi durumu arasında istatistiksel olarak bir ilişki bulunamamıştır (P > 0,05).
Anahtar Kelime: epitel izoenzimler glutatyon transferaz immünohistokimya meme kanseri glutatyon S-transferaz

Konular: Biyoloji

Normal ve tümörleşmiş meme dokusunda GST izozimlerinin ekspresyonlarının karşılaştırılması: klinik ve prognoz faktörlerle ilişkisi

Öz:
Glutathione S-transferases in breast tissue play an important role in the susceptibility to the mutagenic effects of chemical carcinogens and in the response of breast tumors to chemotherapy. In this study the immunohistochemical staining characteristics of glutathione S-transferase isoenzymes (alpha, mu, pi, and theta) were investigated in invasive duct carcinomas and in normal breast tissue of 43 patients. The relationships between the expression of the GST isoenzymes and some clinicopathological features were also examined. Diffuse cytoplasmic staining of varying intensity was observed for GST alpha, theta, and pi in normal and tumorous breast tissue in 100% of the samples. In normal epithelium there was a stronger intensity of staining for GST alpha, mu, and pi expression than in invasive tumor tissues (P < 0.05); however, it was statistically proven that in normal and tumor epithelial cells there was no significant difference in the GST theta isoenzyme staining scores (P > 0.05). In this study significant relationships were observed between microcalcification status and GST mu, between menopause status and GST alpha, and between tumor grade and GST mu expression (P < 0.05). The relationships between GST isoenzyme expression, and estrogen receptor status, tumor grade, smoking status, chemotherapy status, parity, patient&#8217;s age, and hormone therapy status were not statistically significant (P > 0.05).
Anahtar Kelime: isoenzymes glutathione transferase immunohistochemistry breast cancer glutathione S-transferase epithelium

Konular: Biyoloji
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA OĞUZTÜZÜN S, İŞCAN M, OZHAVZALI M, Dizbay Sak S (2009). Comparison of GST ısoenzyme expression in normal and neoplastic breast tissue: correlation with clinical and prognostic factors. , 89 - 100.
Chicago OĞUZTÜZÜN Serpil,İŞCAN Mesude,OZHAVZALI MÜZEYYEN,Dizbay Sak Serpil Comparison of GST ısoenzyme expression in normal and neoplastic breast tissue: correlation with clinical and prognostic factors. (2009): 89 - 100.
MLA OĞUZTÜZÜN Serpil,İŞCAN Mesude,OZHAVZALI MÜZEYYEN,Dizbay Sak Serpil Comparison of GST ısoenzyme expression in normal and neoplastic breast tissue: correlation with clinical and prognostic factors. , 2009, ss.89 - 100.
AMA OĞUZTÜZÜN S,İŞCAN M,OZHAVZALI M,Dizbay Sak S Comparison of GST ısoenzyme expression in normal and neoplastic breast tissue: correlation with clinical and prognostic factors. . 2009; 89 - 100.
Vancouver OĞUZTÜZÜN S,İŞCAN M,OZHAVZALI M,Dizbay Sak S Comparison of GST ısoenzyme expression in normal and neoplastic breast tissue: correlation with clinical and prognostic factors. . 2009; 89 - 100.
IEEE OĞUZTÜZÜN S,İŞCAN M,OZHAVZALI M,Dizbay Sak S "Comparison of GST ısoenzyme expression in normal and neoplastic breast tissue: correlation with clinical and prognostic factors." , ss.89 - 100, 2009.
ISNAD OĞUZTÜZÜN, Serpil vd. "Comparison of GST ısoenzyme expression in normal and neoplastic breast tissue: correlation with clinical and prognostic factors". (2009), 89-100.
APA OĞUZTÜZÜN S, İŞCAN M, OZHAVZALI M, Dizbay Sak S (2009). Comparison of GST ısoenzyme expression in normal and neoplastic breast tissue: correlation with clinical and prognostic factors. Turkish Journal of Biology, 33(2), 89 - 100.
Chicago OĞUZTÜZÜN Serpil,İŞCAN Mesude,OZHAVZALI MÜZEYYEN,Dizbay Sak Serpil Comparison of GST ısoenzyme expression in normal and neoplastic breast tissue: correlation with clinical and prognostic factors. Turkish Journal of Biology 33, no.2 (2009): 89 - 100.
MLA OĞUZTÜZÜN Serpil,İŞCAN Mesude,OZHAVZALI MÜZEYYEN,Dizbay Sak Serpil Comparison of GST ısoenzyme expression in normal and neoplastic breast tissue: correlation with clinical and prognostic factors. Turkish Journal of Biology, vol.33, no.2, 2009, ss.89 - 100.
AMA OĞUZTÜZÜN S,İŞCAN M,OZHAVZALI M,Dizbay Sak S Comparison of GST ısoenzyme expression in normal and neoplastic breast tissue: correlation with clinical and prognostic factors. Turkish Journal of Biology. 2009; 33(2): 89 - 100.
Vancouver OĞUZTÜZÜN S,İŞCAN M,OZHAVZALI M,Dizbay Sak S Comparison of GST ısoenzyme expression in normal and neoplastic breast tissue: correlation with clinical and prognostic factors. Turkish Journal of Biology. 2009; 33(2): 89 - 100.
IEEE OĞUZTÜZÜN S,İŞCAN M,OZHAVZALI M,Dizbay Sak S "Comparison of GST ısoenzyme expression in normal and neoplastic breast tissue: correlation with clinical and prognostic factors." Turkish Journal of Biology, 33, ss.89 - 100, 2009.
ISNAD OĞUZTÜZÜN, Serpil vd. "Comparison of GST ısoenzyme expression in normal and neoplastic breast tissue: correlation with clinical and prognostic factors". Turkish Journal of Biology 33/2 (2009), 89-100.