The frequency of micronuclei and morphological effects on white blood cells following radiotherapy

KURTMAN, Cengiz; ARICA, Çakır Şükran; çavuşoğlu, kültiğin

The frequency of micronuclei and morphological effects on white blood cells following radiotherapy

KÜLTİĞİN ÇAVUŞOĞLU, (Giresun Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Giresun, Türkiye)

Çakır Şükran ARICA, (Kırıkkale Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Kırıkkale, Türkiye)

Cengiz KURTMAN (Ankara Üniversitesi, Tıp Fakültesi, Radyasyon Onkolojisi Anabilim Dalı, Ankara, Türkiye)

Fırat Üniversitesi Sağlık Bilimleri Tıp Dergisi

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Yıl: 2009 Cilt: 23 Sayı: 1 Sayfa Aralığı: 15 - 20 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

The frequency of micronuclei and morphological effects on white blood cells following radiotherapy

Öz:

Bu çalışmada, akciğer tümörleri nedeniyle radyoterapiye (RT) maruz kalan kanser hastalarının periferal kan örneklerindeki morfolojik hasarları ve mikronukleus (MN) sıklığını belirlemeye çalıştık. Bu amaçla, in vivo kümülatif doz-etki ilişkisini kullandık ve bu verileri istatistiksel parametreler ile ilişkilendirdik. Sitolojik analizler, γ–radyasyonuna maruz kalan hastaların beyaz kan hücrelerinde (WBC) gerçekleştirildi. MN testi, radyasyon hasarının bir belirteci olarak kullanıldı. Ayrıca, sigara alışkanlığı ve yaş gibi farklı faktörlerin etkileride MN testi kullanılarak araştırıldı. MN sayımı, tek nükleuslu lenfositlerde gerçekleştirildi. WBC'nin sayısı ve morfolojik özelliklerini belirlemek amacıyla periferal kan yayma preparatları hazırlandı. Sonuçlar, γ–radyasyonuna maruz kalan kanser hastalarının WBC'deki MN sıklığı ve morfolojik hasarların, kontrol grubundakilerden oldukça yüksek olduğunu gösterdi ve fark istatistiksel olarak önemliydi (P<0.01). WBC'de voküolizasyon, membran kusurları ve sitoplazmik granülasyonda artış gibi morfolojik değişmeler belirlendi. Ayrıca, radyasyon dozuna bağlı olarak MN sıklığında bir artış ve WBC'nin sayısında ise bir azalma gözlendi. Sonuç olarak, morfolojik hasarlar ve MN sıklığının, RT 'nin etkilerini araştırmak için çok hassas ve kullanışlı biyolojik belirteçler olduğu gösterildi.

Anahtar Kelime:

Konular: Biyoloji

Radyoterapiyi takiben beyaz kan hücrelerindeki morfolojik etkiler ve mikronukleus sıklığı

Öz:

In this study, we tried to define the frequency of micronuclei (MN) and morphological damages in peripheral blood samples of cancer patients exposed to radiotherapy (RT) for lung tumors. For this purpose, we used in vivo cumulative dose-effect relationship, and correlated these data with statistical parameters. Cytological analyses were performed on white blood cells (WBC) of patients exposed to γ–radiation. MN assay was used as a biomarker of radiation damage. Besides, the effect of different factors like smoking status and age was investigated by MN assay. Scoring of MN was performed on mononucleated lymphocytes. Peripheral blood smears were prepared for morphological characterization and the number of WBC. The results indicated that, morphological damages and MN frequency in WBC were significantly higher in cancer patients exposed to γ–radiation than in the controls, and difference was statistically significant (P<0.01). The morphological changes such as vacuolization, membrane defects and increase in cytoplasmic granulation were detected in WBC. Moreover, an increase in the frequency of MN and a decrease in the numbers of WBC were observed depending on radiation dose. In conclusion, it was showed that morphological damages and MN frequency are very sensitive and useful biomarkers for the investigation of the effects of RT.

Anahtar Kelime:

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	çavuşoğlu k, ARICA Ç, KURTMAN C (2009). The frequency of micronuclei and morphological effects on white blood cells following radiotherapy. , 15 - 20.
Chicago	çavuşoğlu kültiğin,ARICA Çakır Şükran,KURTMAN Cengiz The frequency of micronuclei and morphological effects on white blood cells following radiotherapy. (2009): 15 - 20.
MLA	çavuşoğlu kültiğin,ARICA Çakır Şükran,KURTMAN Cengiz The frequency of micronuclei and morphological effects on white blood cells following radiotherapy. , 2009, ss.15 - 20.
AMA	çavuşoğlu k,ARICA Ç,KURTMAN C The frequency of micronuclei and morphological effects on white blood cells following radiotherapy. . 2009; 15 - 20.
Vancouver	çavuşoğlu k,ARICA Ç,KURTMAN C The frequency of micronuclei and morphological effects on white blood cells following radiotherapy. . 2009; 15 - 20.
IEEE	çavuşoğlu k,ARICA Ç,KURTMAN C "The frequency of micronuclei and morphological effects on white blood cells following radiotherapy." , ss.15 - 20, 2009.
ISNAD	çavuşoğlu, kültiğin vd. "The frequency of micronuclei and morphological effects on white blood cells following radiotherapy". (2009), 15-20.

APA	çavuşoğlu k, ARICA Ç, KURTMAN C (2009). The frequency of micronuclei and morphological effects on white blood cells following radiotherapy. Fırat Üniversitesi Sağlık Bilimleri Tıp Dergisi, 23(1), 15 - 20.
Chicago	çavuşoğlu kültiğin,ARICA Çakır Şükran,KURTMAN Cengiz The frequency of micronuclei and morphological effects on white blood cells following radiotherapy. Fırat Üniversitesi Sağlık Bilimleri Tıp Dergisi 23, no.1 (2009): 15 - 20.
MLA	çavuşoğlu kültiğin,ARICA Çakır Şükran,KURTMAN Cengiz The frequency of micronuclei and morphological effects on white blood cells following radiotherapy. Fırat Üniversitesi Sağlık Bilimleri Tıp Dergisi, vol.23, no.1, 2009, ss.15 - 20.
AMA	çavuşoğlu k,ARICA Ç,KURTMAN C The frequency of micronuclei and morphological effects on white blood cells following radiotherapy. Fırat Üniversitesi Sağlık Bilimleri Tıp Dergisi. 2009; 23(1): 15 - 20.
Vancouver	çavuşoğlu k,ARICA Ç,KURTMAN C The frequency of micronuclei and morphological effects on white blood cells following radiotherapy. Fırat Üniversitesi Sağlık Bilimleri Tıp Dergisi. 2009; 23(1): 15 - 20.
IEEE	çavuşoğlu k,ARICA Ç,KURTMAN C "The frequency of micronuclei and morphological effects on white blood cells following radiotherapy." Fırat Üniversitesi Sağlık Bilimleri Tıp Dergisi, 23, ss.15 - 20, 2009.
ISNAD	çavuşoğlu, kültiğin vd. "The frequency of micronuclei and morphological effects on white blood cells following radiotherapy". Fırat Üniversitesi Sağlık Bilimleri Tıp Dergisi 23/1 (2009), 15-20.