Yıl: 2010 Cilt: 16 Sayı: 5 Sayfa Aralığı: 751 - 756 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

The effects of bacterial inoculants and/or enzymes on the fermentation, aerobic stability and in vitro dry and organic matter digestibility characteristics of Triticale silages

Öz:
Bu çalışma silaj katkı maddesi olarak kullanılan laktik asit bakteri inokulantı, enzim ve laktik asit bakteri+enzim karışımı inokulantların, tritikale (xTriticosecale Wittmack) silajlarının fermantasyon, aerobik stabilite ve in vitro kuru ve organik madde sindirilebilirlik özellikleri üzerindeki etkilerinin saptanması amacı ile düzenlenmiştir. Laktik asit bakteri inokulantı olarak Pioneer1188 (Iowa, USA), enzim (Global Nutritech, TR) ve laktik asit bakteri+enzim karışımı inokulant olarak Sil-All (Allteck, UK) kullanılmıştır. İnokulantlar silajlara 6.00 log10 cfu/g düzeyinde katılmışlardır. Uygulamadan sonra parçalanmış tritikale, yalnızca gaz çıkışına olanak tanıyan 1.0 litrelik özel anaerobik kavanozlara silolanmıştır. Silolamadan sonraki 2, 5, 8 ve 45. günlerde her gruptan üçer kavanoz açılarak kimyasal ve mikrobiyolojik analizler yapılmıştır. Silolama döneminin sonunda (45. gün) açılan tüm silajlara 5 gün süre ile aerobik stabilite testi uygulanmıştır. Ayrıca bu silajların, in vitro kuru ve organik madde sindirilebilirlikleri saptanmıştır. Sonuç olarak her iki inokulant ve enzim, tritikale silajlarının fermantasyon özelliklerini artırmıştır. Enzim ve laktik asit bakteri+enzim karışımı inokulantları silajların nötral deterjanlarda çözünmeyen lif içeriklerini azaltmış ve in vitro kuru ve organik madde sindirilebilirliklerini artırmıştır.
Anahtar Kelime: hücre duvarı bileşenleri fermentasyon enzimler sindirilebilirlik silaj enzim aktivitesi silaj kalitesi Secale x Triticum organik madde tritikale aerobik işlemler silaj katkı maddeleri

Konular: Veterinerlik

Bakteriyal inokulantlar ve/veya enzimlerin Tritikale silajlarında fermantasyon, aerobik stabilite ve in vitro kuru ve organik madde sindirilebilirliği üzerine etkileri

Öz:
This study was carried out to determine the effects of lactic acid bacteria inoculant, enzymes and lactic acid bacteria inoculant-enzymes mixture on the fermentation, cell wall content, aerobic stability and in vitro dry and organic matter digestibility characteristics of triticale (xTriticosecale Wittmack) silages. Triticale was harvested at the milk stage of maturity. Pioneer -1188 (Iowa, USA), enzyme (Global Nutritech, TR) and Sil-All (Alltech, UK) were used as lactic acid bacteria, enzyme and lactic acid bacteria+enzyme mixture inoculants. Inoculants were applied to silages at 6.00 log10 cfu/g levels. After treatment, the chopped triticale was ensiled in 1.0 liter special anaerobic jars, equipped with a lid enabling gas release only. Three jars from each group were sampled for chemical and microbiological analysis on days 2, 5, 8 and 45 after ensiling. At the end of the ensiling period ($45^ {th}$ day) all silages were subjected to an aerobic stability test for 5 days. In addition, in vitro dry and organic matter digestibility of these silages were determined. Both inoculants and enzymes increased characteristics of fermentation but impaired aerobic stability of triticale silages. Enzymes and lactic acid bacteria+enzymes mixture inoculant decreased neutral detergent fibre content and increased in vitro dry and organic matter digestibility of silages.
Anahtar Kelime: silage enzyme activity silage quality Secale x Triticum organic matter triticale aerobic treatment silage additives cell wall components fermentation enzymes digestibility

Konular: Veterinerlik
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • 1.Oshita T, Takayama H, Otsuka H, Igarashi H, Nonaka K, Kume S: Effects of maturing stage of corn hybrids on silage yield, feeding value for dairy cows and milk production in a cold region of Japan. Asian-Aust J Anim Sci, 20, 511-516, 2007.
  • 2.Vatandoost M, Danesh Mesgaran M, Valizadeh R, Nasiri Moghaddam H: Effect of whole crop silages (triticale or barley) versus corn silage on performance of Holstein lactating dairy cows. J Anim Vet Advances, 6, 344-348, 2007.
  • 3.van Duinkerken G, Zom RLG, Bleumer EJB: The effects of replacing maize silage by triticale whole crop silage in a roughage mixture with grass silage on feed intake and milk production by dairy cows. Proceedings of the British Society of Animal Science Annual Meeting. p. 78. Scarborough, 1999.
  • 4.Cai YM, Benno Y, Ogawa M, Ohmomo S, Kumai S, Nakase T: Influence of Lactobacillus spp. from an inoculant and of Weissella and Leuconostoc spp. from forage crops on silage fermentation. Appl Environ Microbiol, 64, 2982-2987, 1998.
  • 5.Filya İ: Silaj kalitesinin arttırılmasında yeni gelişmeler. International Animal Nutrition Congress’2000, s. 243-250. Isparta. 2000.
  • 6.Sucu E, Filya I: The effects of bacterial inoculants on the fermentation, aerobic stability and rumen degradability characteristics of wheat silages. Turk J Vet Anim Sci, 30, 187193, 2006.
  • 7.Sheperd AC, Kung L: An enzyme additive for corn silage: Effects on silage composition and animal performance. J Dairy Sci, 79, 1760-1766, 1996.
  • 8.Chen J, Stokes MR, Wallace CR: Effects of enzymeinoculant systems on preservation and nutritive value of hay crop and corn silages. J Dairy Sci, 77, 501-512, 1994.
  • 9.Guedes CM, Rodrigues MAM, Gomes MJ, Silva SR, Ferreira LM, Mascarenhas-Ferreira A: Urea treatment of whole-crop triticale at four growth stages: Effects on chemical composition and on in vitro digestibility of cell wall. J Sci Food Agr, 86, 964-970, 2006.
  • 10.Nadeau E: Effects of plant species, stage of maturity and additive on the feeding value of whole-crop cereal silage. J Sci Food Agric, 87, 789-801, 2007.
  • 11.Ashbell G, Weinberg ZG, Azrieli A, Hen Y, Horev B: A simple system to study the aerobic deterioration of silages. Can Agric Eng, 33, 391-393, 1991.
  • 12.Anonymus: The Analysis of Agricultural Material. Reference Book. pp. 427-428, London. 1986.
  • 13.Koç F, Coşkuntuna L: Silo yemlerinde organik asit belirlemede iki farklı metodun karşılaştırılması. J Anim Prod, 44, 37-47, 2003.
  • 14.Seale DR, Pahlow G, Spoelstra SF, Lindgren S, Dellaglio F, Lowe JF: Methods for the microbiological analysis of silage. Proceeding of The Eurobac Conference, 147, Uppsala, 1990.
  • 15.Filya I: The effect of Lactobacillus buchneri and Lactobacillus plantarum on the fermentation, aerobic stability, and ruminal degradability of low dry matter corn and sorghum silages. J Dairy Sci, 86, 3575-3581, 2003.
  • 16.AOAC: Official Methods of Analysis. 15th ed., Association of Official Analytical Chemists, Arlington, Virginia, USA, 1990.
  • 17.Goering HK, Van Soest PJ: Forage Fiber Analyses. Agricultural Handbook, No 379, Washington. 1983.
  • 18.Aufrère J, Michalet-Doreau B: Comparison of methods for predicting digestibility of feeds. Anim Feed Sci Technol, 20, 203-218, 1988.
  • 19.MINITAB: Minitab Incoporation. Minitab for Windows, Release 13 for Windows. User’s Guide 2-Data Analysis and Quality Tools, Minitab Inc, USA, 2000.
  • 20.Sucu E, Filya I: Effects of homofermentative lactic acid bacterial inoculants on the fermentation and aerobic stability characteristics of low dry matter corn silages. Turk J Vet Anim Sci, 30, 83-88, 2006.
  • 21.Williams CC, Froetschel MA, Ely LO, Amos HE: Effects of inoculation and wilting on the preservation and utilization of wheat forage. J Dairy Sci, 78, 1755-1765, 1995.
  • 22.Zahiroddini H, Baah J, Absalom W, McAllister TA: Effect of an inoculant and hydrolytic enzymes on fermentation and nutritive value of whole crop barley silage. Anim Feed Sci Technol, 117, 317-330, 2004.
  • 23.Kung LM, Shaver R: Interpretation and use of silage fermentation analysis reports. Focus Forage, 3, 1-5, 2001.
  • 24.Xing L, Chen J, Han LJ: The effect of an inoculant and enzymes on fermentation and nutritive value of sorghum straw silages. Bioresour Technol, 100, 488-491, 2009.
  • 25.Jaakkola S: The effect of cell wall degrading enzymes on the preservation of grass and on the silage intake and digestibility in sheep. J Agric Sci Finland, 62, 51-62, 1990.
  • 26.Selmer-Olsen I, Henderson AR, Robertson S, McGinn R: Cell wall degrading enzymes for silage. 1. The fermentation of enzyme-treated ryegrass in laboratory silos. Grass Forage Sci, 48, 45-54, 1993.
  • 27. McDonald P, Henderson AR, Heron SJE: The Biochemistry of Silage. Chalcombe Publications, 13 Highwoods drive, Marlow Bottom, Marlow, Bucks, UK, 1991.
  • 28. Weinberg ZG, Ashbell G, Yaira H, Azrieli A: The effect of cellulase and hemicellulase plus pectinase on the aerobic stability and fiber analysis of peas and wheat silages. Anim Feed Sci Technol, 55, 287-293, 1995.
  • 29. Ozduven ML, Koc F, Polat C, Coskuntuna L: The effects of lactic acid bacteria and enzyme mixture inoculants on fermentation and nutrient digestibility of sunflower silage. Kafkas Univ Vet Fak Derg, 15(2): 195-199, 2009.
  • 30. Sheperd AC, Maslanka M, Quinn D, Kung L: Additives containing bacteria and enzymes for alfalfa silage. J Dairy Sci, 78, 565-572, 1995.
  • 31. Nadeau EMG, Buxton DR, Russell JR, Allison MJ, Young JW: Enzyme, bacterial inoculant, and formic acid effects on silage composition of orchardgrass and alfalfa. J Dairy Sci, 83, 1487-1502, 2000.
  • 32. Nadeau EMG, Russell JR, Buxton DR: Intake, digestibility, and composition of orchardgrass and alfalfa silages treated with cellulase, inoculant, and formic acid fed to lambs. J Animal Sci, 78, 2980-2989, 2000.
  • 33. Filya I: Laktik asit bakteri ve laktik asit bakteri + enzim karışımı silaj inokulantlarının mısır silajı üzerine etkileri. Turk J Vet Animal Sci, 26, 679-687, 2002.
  • 34. Colombatto D, Mould FL, Bhat MK, Morgavi DP, Beauchemin KA, Owen E: Influence of fibrolytic enzymes on the hydrolysis and fermentation of pure cellulose and xylan by mixed ruminal microorganisms in vitro. J Anim Sci, 81, 1040-1050, 2003.
  • 35. Polat C, Koç F, Özdüven ML: Mısır silajlarnda laktik asit bakterileri ve laktik asit bakteri+enzim karışımı inokulantların fermantasyon ve toklularda ham besin maddelerinin sindirilme dereceleri üzerine etkileri. J Tekirdag Agricultural Faculty, 2, 13-22, 2005.
  • 36. Kung L, Tung RS, Maciorowski KG, Buffum K, Knutsen K, Aimutis WR: Effects of plant cell-wall-degrading enzymes and lactic acid bacteria on silage fermentation and composition. J Dairy Sci, 74, 4284-4296, 1991.
  • 37. Meeske R, Basson HM, Cruywagen CW: The effects of a lactic acid bacteria inoculant with enzymes on the fermentation dynamics, intake and digestibility of Digitaria eriantha silage. Anim Feed Sci Technol, 81, 237-248, 1999.
  • 38. Weinberg ZG, Szakacs G, Ashbell G, Hen Y: The effect of temperature on the ensiling process of corn and wheat. J Appl Microbiol, 90, 561-566, 2001.
  • 39. Meeske R, Ashbell G, Weinberg ZG, Kipnis T: Ensiling forage sorghum at two stages of maturity with the addition of lactic acid bacterial inoculants. Anim Feed Sci Technol, 43, 165-175, 1993.
  • 40. Hristov AN, McAllister TA: Effect of inoculants on whole- crop barley silage fermentation and dry matter disappearance in situ. J Anim Sci, 80, 510-516, 2002.
APA OZDUVEN M, ONAL Z, KOC F (2010). The effects of bacterial inoculants and/or enzymes on the fermentation, aerobic stability and in vitro dry and organic matter digestibility characteristics of Triticale silages. , 751 - 756.
Chicago OZDUVEN MEHMET LEVENT,ONAL Zeynep Kursun,KOC Fisun The effects of bacterial inoculants and/or enzymes on the fermentation, aerobic stability and in vitro dry and organic matter digestibility characteristics of Triticale silages. (2010): 751 - 756.
MLA OZDUVEN MEHMET LEVENT,ONAL Zeynep Kursun,KOC Fisun The effects of bacterial inoculants and/or enzymes on the fermentation, aerobic stability and in vitro dry and organic matter digestibility characteristics of Triticale silages. , 2010, ss.751 - 756.
AMA OZDUVEN M,ONAL Z,KOC F The effects of bacterial inoculants and/or enzymes on the fermentation, aerobic stability and in vitro dry and organic matter digestibility characteristics of Triticale silages. . 2010; 751 - 756.
Vancouver OZDUVEN M,ONAL Z,KOC F The effects of bacterial inoculants and/or enzymes on the fermentation, aerobic stability and in vitro dry and organic matter digestibility characteristics of Triticale silages. . 2010; 751 - 756.
IEEE OZDUVEN M,ONAL Z,KOC F "The effects of bacterial inoculants and/or enzymes on the fermentation, aerobic stability and in vitro dry and organic matter digestibility characteristics of Triticale silages." , ss.751 - 756, 2010.
ISNAD OZDUVEN, MEHMET LEVENT vd. "The effects of bacterial inoculants and/or enzymes on the fermentation, aerobic stability and in vitro dry and organic matter digestibility characteristics of Triticale silages". (2010), 751-756.
APA OZDUVEN M, ONAL Z, KOC F (2010). The effects of bacterial inoculants and/or enzymes on the fermentation, aerobic stability and in vitro dry and organic matter digestibility characteristics of Triticale silages. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 16(5), 751 - 756.
Chicago OZDUVEN MEHMET LEVENT,ONAL Zeynep Kursun,KOC Fisun The effects of bacterial inoculants and/or enzymes on the fermentation, aerobic stability and in vitro dry and organic matter digestibility characteristics of Triticale silages. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 16, no.5 (2010): 751 - 756.
MLA OZDUVEN MEHMET LEVENT,ONAL Zeynep Kursun,KOC Fisun The effects of bacterial inoculants and/or enzymes on the fermentation, aerobic stability and in vitro dry and organic matter digestibility characteristics of Triticale silages. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.16, no.5, 2010, ss.751 - 756.
AMA OZDUVEN M,ONAL Z,KOC F The effects of bacterial inoculants and/or enzymes on the fermentation, aerobic stability and in vitro dry and organic matter digestibility characteristics of Triticale silages. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2010; 16(5): 751 - 756.
Vancouver OZDUVEN M,ONAL Z,KOC F The effects of bacterial inoculants and/or enzymes on the fermentation, aerobic stability and in vitro dry and organic matter digestibility characteristics of Triticale silages. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2010; 16(5): 751 - 756.
IEEE OZDUVEN M,ONAL Z,KOC F "The effects of bacterial inoculants and/or enzymes on the fermentation, aerobic stability and in vitro dry and organic matter digestibility characteristics of Triticale silages." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 16, ss.751 - 756, 2010.
ISNAD OZDUVEN, MEHMET LEVENT vd. "The effects of bacterial inoculants and/or enzymes on the fermentation, aerobic stability and in vitro dry and organic matter digestibility characteristics of Triticale silages". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 16/5 (2010), 751-756.