Effect of physical activity on plasma adrenomedullin concentration and its relationship with nitric oxide in athletes

DURSUN, Nurcan; ÖZDOĞAN, Kalender; Yurekli, Muhittin

Effect of physical activity on plasma adrenomedullin concentration and its relationship with nitric oxide in athletes

Nurcan DURSUN, (Erciyes Üniversitesi, Tıp Fakültesi, Fizyoloji Anabilim Dalı, Kayseri, Türkiye)

Muhittin YÜREKLİ, (İnönü Üniversitesi, Tıp Fakültesi, Moleküller Biyoloji Anabilim Dalı, Malatya, Türkiye)

Kalender ÖZDOĞAN (Erciyes Üniversitesi, Tıp Fakültesi, Fizyoloji Anabilim Dalı, Kayseri, Türkiye)

Erciyes Tıp Dergisi

1 0

Yıl: 2012 Cilt: 34 Sayı: 1 Sayfa Aralığı: 1 - 6 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Effect of physical activity on plasma adrenomedullin concentration and its relationship with nitric oxide in athletes

Öz:

Amaç: Bu çalışmanın amacı düzenli fiziksel aktivite ya da akut egzersizin plazma adrenomedullin (AM) konsantrasyonunu etkileyip etkilemediğini ve AM’in cinsiyet kan basıncı ve nitrik oksit (NO) yapımı ile ilişkisini araştırmaktır. Gereç ve Yöntem: Bu çalışmaya 60 sağlıklı öğrenci katıldı. Bunlar iki guruba ayrıldı. Düzenli spor yapan 16 kız 14 erkek toplam 30 öğrenci birinci gurubu oluşturdu. Sporcu olmayan 15 kız 15 erkekte ikinci gurubu oluşturdu. Öğrenciler bitkinlik oluşuncaya kadar bisiklet egzersiz testine tabi tutuldu. Kalp hızı, kan basıncı, maksimal O2 tüketimi (VO2max) ve CO2 üretimi (VCO2) egzersiz boyunca ölçüldü. Akut egzersize başlamadan önce, birinci ve 30. dakikasında öğrencilerden AM ve NO seviyelerinin tesbiti için venöz kan örnekleri alındı. Bulgular: Kız ve erkek sporcuların AM seviyesi, sporcu olmayanlarınkinden önemli derecede yüksekti. Kız sporcuların plazma AM konsantrasyonları erkek sporcularınkinden önemli derecede farklı bulundu. Kişilerdeki AM konsantrasyonunu akut egzersiz etkilemedi AMBMI (vücut kitle indeksi) ile sistolik- diyastolik basıçlar, kalp hızı VO2max ve VCO2 arasında önemli bir ilişki bulunmadı. Sonuç: Bu çalışmadaki veriler, düzenli fiziksel aktivite esnasında artmış AM’nin, kişilerin kan basıncındaki yükselmeyi düzenleyen kompensatuvar bir mekanizma olabileceğini göstermektedir.

Anahtar Kelime:

Konular: Genel ve Dahili Tıp

Sporcularda adrenomedullin konsantrasyonu üzerine fiziksel aktivitenin etkisi ve nitrik oksit ile ilişkisi

Öz:

Objective: The aim of this study was to determine whether regular physical activity or acute exercise influenced plasma adrenomedullin (AM) concentration and whether it was related to gender, blood pressure and nitric oxide (NO) production. Material and Methods: Sixty healthy students participated in this study. They were divided into two groups. The first group consisted of 30 students, 16 female, 14 male athletes carrying out regular exercise. The second group was made up of 15 females, 15 males nonathletes. All students submitted to a cycle exercise test until volitional exhaustion. Heart rate (HR), blood pressure, maximal oxygen uptake (VO2max) and carbon dioxide production (VCO2) were measured continously. Before starting, at the 1st min and at the 30th min of exercise, venous blood samples were taken to determine AM and NO production. Results: AM in the female and male athletes were significantly higher than in the female and male non-athletes. The plasma AM concentration in female athletes was found be significantly higher than in male athletes. Acute exercise did not affect AM concentration in subjects. We did not find any relationship between AM/BMI (Body mass index) and other variables such as systolic, diastolic pressure, heart rate, VO2max and VCO2 but there was a negative relationship with NO production. Conclusion: The present data suggest that an increase in AM during prolonged physical activity may be a compensatory mechanism against further elevation of blood pressure.

Anahtar Kelime:

Konular: Genel ve Dahili Tıp

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Kitamura K, Kangawa K, Kawamoto M, Ichiki Y, Nakamura S, Matsuo H, et al. Adrenomedullin: a novel hypotensive peptide isolated from human pheochromocytoma. Biochem Biophys Res Commun 1993; 192: 553-60. [CrossRef]
2. Kitamura K, Sakata J, Kangawa K, Kojima M, Matsuo H, Eto T. Cloning and characterization of cDNA encoding a precursor for human adrenomedullin. Biochem Biophys Res Commun 1993; 194: 720-5. [CrossRef]
3. Beltowski J, Jamroz A. Adrenomedullin--what do we know 10 years since its discovery? Pol J Pharmacol 2004; 56: 5-27.
4. Nishikimi T, Yoshihara F, Mori Y, Kangawa K, Matsuoka H. Cardioprotective effect of adrenomedullin in heart failure. Hypertens Res 2003; 26 Suppl: S121-7. [CrossRef]
5. Ishimitsu T, Nishikimi T, Saito Y, Kitamura K, Eto T, Kangawa K, et al. Plasma levels of adrenomedullin, a newly identified hypotensive peptide, in patients with hypertension and renal failure. J Clin Invest 1994; 94: 2158-61. [CrossRef]
6. Jougasaki M, Wei CM, McKinley LJ, Burnett JC, Jr. Elevation of circulating and ventricular adrenomedullin in human congestive heart failure. Circulation 1995; 92: 286-9.
7. Miyao Y, Nishikimi T, Goto Y, Miyazaki S, Daikoku S, Morii I, et al. Increased plasma adrenomedullin levels in patients with acute myocardial infarction in proportion to the clinical severity. Heart 1998; 79: 39-44.
8. Nicholls MG, Charles CJ, Lainchbury JG, Lewis LK, Rademaker MT, Richards AM, et al. Adrenomedullin in heart failure. Hypertens Res 2003; 26 Suppl: S135-40. [CrossRef]
9. Eto T, Kato J, Kitamura K. Regulation of production and secretion of adrenomedullin in the cardiovascular system. Regul Pept 2003; 112 61-9. [CrossRef]
10. Oie E, Vinge LE, Yndestad A, Sandberg C, Grogaard HK, Attramadal H. Induction of a myocardial adrenomedullin signaling system during ischemic heart failure in rats. Circulation 2000; 101: 415-22.
11. Pan CS, Jin SJ, Cao CQ, Zhao J, Zhang J, Wang X, et al. The myocardial response to adrenomedullin involves increased cAMP generation as well as augmented Akt phosphorylation. Peptides 2007; 28: 900-9. [CrossRef]
12. Orshal JM, Khalil RA. Gender, sex hormones, and vascular tone. Am J Physiol Regul Integr Comp Physiol 2004; 286: R233-49. [CrossRef]
13. Sugo S, Minamino N, Kangawa K, Miyamoto K, Kitamura K, Sakata J, et al. Endothelial cells actively synthesize and secrete adrenomedullin. Biochem Biophys Res Commun 1994; 201: 1160-6. [CrossRef]
14. Eto T, Kitamura K, Kato J. Biological and clinical roles of adrenomedullin in circulation control and cardiovascular diseases. Clin Exp Pharmacol Physiol 1999; 26: 371-80. [CrossRef]
15. Neri G, Bova S, Malendowicz LK, Mazzocchi G, Nussdorfer GG. Simulated microgravity impairs aldosterone secretion in rats: possible involvement of adrenomedullin. Am J Physiol Regul Integr Comp Physiol 2002; 283: R832-6.
16. Tanaka M, Kitamura K, Ishizaka Y, Ishiyama Y, Kato J, Kangawa K, et al. Plasma adrenomedullin in various diseases and exercise-induced change in adrenomedullin in healthy subjects. Intern Med 1995; 34: 728-33. [CrossRef]
17. Piquard F, Charloux A, Mettauer B, Epailly E, Lonsdorfer E, Popescu S, et al. Exercise-induced increase in circulating adrenomedullin is related to mean blood pressure in heart transplant recipients. J Clin Endocrinol Metab 2000; 85: 2828-31. [CrossRef]
18. Nishikimi T, Morimoto A, Ishikawa K, Saito Y, Kangawa K, Matsuo H, et al. Different secretion patterns of adrenomedullin, brain natriuretic peptide, and atrial natriuretic peptide during exercise in hypertensive and normotensive subjects. Clin Exp Hypertens 1997; 19: 503-18. [CrossRef]
19. Morimoto A, Nishikimi T, Takaki H, Okano Y, Matsuoka H, Takishita S, et al. Effect of exercise on plasma adrenomedullin and natriuretic peptide levels in myocardial infarction. Clin Exp Pharmacol Physiol 1997; 24: 315-20. [CrossRef]
20. Poveda JJ, Berrazueta JR, Ochoteco A, Montalban C, Garcia-Unzueta MT, Fernandez C, et al. Age-related responses of vasoactive factors during acute exercise. Horm Metab Res 1998; 30: 668-72. [CrossRef]
21. Krzeminski K, Mikulski T, Nazar K. Effect of prolonged dynamic exercise on plasma adrenomedullin concentration in healthy young men. J Physiol Pharmacol 2006; 57: 571-81.
22. Krzeminski K, Kruk B, Wojcik-Ziolkowska E, Kozera J, Cybulski G, Nazar K. Effect of static handgrip on plasma adrenomedullin concentration in patients with heart failure and in healthy subjects. J Physiol Pharmacol 2002; 53: 199-210.
23. Krzeminski K, Mikulski T, Kruk B, Nazar K. Plasma adrenomedullin response to maximal exercise in healthy subjects. J Physiol Pharmacol 2003; 54: 225-32.
24. Dikensoy E, Balat O, Pence S, Balat A, Cekmen M, Yurekli M. The changes of plasma malondialdehyde, nitric oxide, and adrenomedullin levels in patients with preeclampsia. Hypertens Pregnancy 2009; 28: 383-9. [CrossRef]
25. Fowler DE, Wang P. The cardiovascular response in sepsis: proposed mechanisms of the beneficial effect of adrenomedullin and its binding protein (review). Int J Mol Med 2002; 9: 443-9.
26. Nassis GP, Geladas ND. Cardiac output decline in prolonged dynamic exercise is affected by the exercise mode. Pflugers Arch 2002; 445: 398-404. [CrossRef]
27. Imai T, Hirata Y, Iwashina M, Marumo F. Hormonal regulation of rat adrenomedullin gene in vasculature. Endocrinology 1995; 136: 1544-8. [CrossRef]
28. Isumi Y, Shoji H, Sugo S, Tochimoto T, Yoshioka M, Kangawa K, et al. Regulation of adrenomedullin production in rat endothelial cells. Endocrinology 1998; 139: 838-46. [CrossRef]
29. Pearson LJ, Rait C, Nicholls MG, Yandle TG, Evans JJ. Regulation of adrenomedullin release from human endothelial cells by sex steroids and angiotensin-II. J Endocrinol 2006; 191: 171-7. [CrossRef]
30. Weltman A, Pritzlaff CJ, Wideman L, Considine RV, Fryburg DA, Gutgesell ME, et al. Intensity of acute exercise does not affect serum leptin concentrations in young men. Med Sci Sports Exerc 2000; 32: 1556-61. [CrossRef]
31. Noland RC, Baker JT, Boudreau SR, Kobe RW, Tanner CJ, Hickner RC, et al. Effect of intense training on plasma leptin in male and female swimmers. Med Sci Sports Exerc 2001; 33: 227-31. [CrossRef]
32. Kato J, Tsuruda T, Kita T, Kitamura K, Eto T. Adrenomedullin: a protective factor for blood vessels. Arterioscler Thromb Vasc Biol 2005; 25: 2480-7. [CrossRef]
33. Hattori Y, Nakanishi N, Gross SS, Kasai K. Adrenomedullin augments nitric oxide and tetrahydrobioptein synthesis in cytokine-stimulated vascular smooth muscle cells. Cardiovasc Res 1999; 44: 207-14. [CrossRef]
34. Abe M, Sata M, Nishimatsu H, Nagata D, Suzuki E, Terauchi Y, et al. Adrenomedullin augments collateral development in response to acute ischemia. Biochem Biophys Res Commun 2003; 306: 10-5. [CrossRef]

APA	DURSUN N, Yurekli M, ÖZDOĞAN K (2012). Effect of physical activity on plasma adrenomedullin concentration and its relationship with nitric oxide in athletes. , 1 - 6.
Chicago	DURSUN Nurcan,Yurekli Muhittin,ÖZDOĞAN Kalender Effect of physical activity on plasma adrenomedullin concentration and its relationship with nitric oxide in athletes. (2012): 1 - 6.
MLA	DURSUN Nurcan,Yurekli Muhittin,ÖZDOĞAN Kalender Effect of physical activity on plasma adrenomedullin concentration and its relationship with nitric oxide in athletes. , 2012, ss.1 - 6.
AMA	DURSUN N,Yurekli M,ÖZDOĞAN K Effect of physical activity on plasma adrenomedullin concentration and its relationship with nitric oxide in athletes. . 2012; 1 - 6.
Vancouver	DURSUN N,Yurekli M,ÖZDOĞAN K Effect of physical activity on plasma adrenomedullin concentration and its relationship with nitric oxide in athletes. . 2012; 1 - 6.
IEEE	DURSUN N,Yurekli M,ÖZDOĞAN K "Effect of physical activity on plasma adrenomedullin concentration and its relationship with nitric oxide in athletes." , ss.1 - 6, 2012.
ISNAD	DURSUN, Nurcan vd. "Effect of physical activity on plasma adrenomedullin concentration and its relationship with nitric oxide in athletes". (2012), 1-6.

APA	DURSUN N, Yurekli M, ÖZDOĞAN K (2012). Effect of physical activity on plasma adrenomedullin concentration and its relationship with nitric oxide in athletes. Erciyes Tıp Dergisi, 34(1), 1 - 6.
Chicago	DURSUN Nurcan,Yurekli Muhittin,ÖZDOĞAN Kalender Effect of physical activity on plasma adrenomedullin concentration and its relationship with nitric oxide in athletes. Erciyes Tıp Dergisi 34, no.1 (2012): 1 - 6.
MLA	DURSUN Nurcan,Yurekli Muhittin,ÖZDOĞAN Kalender Effect of physical activity on plasma adrenomedullin concentration and its relationship with nitric oxide in athletes. Erciyes Tıp Dergisi, vol.34, no.1, 2012, ss.1 - 6.
AMA	DURSUN N,Yurekli M,ÖZDOĞAN K Effect of physical activity on plasma adrenomedullin concentration and its relationship with nitric oxide in athletes. Erciyes Tıp Dergisi. 2012; 34(1): 1 - 6.
Vancouver	DURSUN N,Yurekli M,ÖZDOĞAN K Effect of physical activity on plasma adrenomedullin concentration and its relationship with nitric oxide in athletes. Erciyes Tıp Dergisi. 2012; 34(1): 1 - 6.
IEEE	DURSUN N,Yurekli M,ÖZDOĞAN K "Effect of physical activity on plasma adrenomedullin concentration and its relationship with nitric oxide in athletes." Erciyes Tıp Dergisi, 34, ss.1 - 6, 2012.
ISNAD	DURSUN, Nurcan vd. "Effect of physical activity on plasma adrenomedullin concentration and its relationship with nitric oxide in athletes". Erciyes Tıp Dergisi 34/1 (2012), 1-6.