Distribution of plasma cells and alpha-naphthyl acetate esterase – positive lymphocytes in the reproductive organs of female cats in anoestrus, oestrus and pseudopregnancy

KALENDER, Hakan; PİR YAĞCI, İLKNUR; ARIKAN, Şevket; KARACA, Turan; YORUK, MECIT

Distribution of plasma cells and alpha-naphthyl acetate esterase – positive lymphocytes in the reproductive organs of female cats in anoestrus, oestrus and pseudopregnancy

Turan KARACA, (Trakya Üniversitesi, Veteriner Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Tekirdağ, Türkiye)

Şevket ARIKAN, (Kırıkkale Üniversitesi, Veteriner Fakültesi, Fizyoloji Anabilim Dalı, Kırıkkale, Türkiye)

Hakan KALENDER, (Kırıkkale Üniversitesi, Veteriner Fakültesi, Doğum ve Jinekoloji Anabilim Dalı, Kırıkkale, Türkiye)

İLKNUR PİR YAĞCI, (Kırıkkale Üniversitesi, Veteriner Fakültesi, Doğum ve Jinekoloji Anabilim Dalı, Kırıkkale, Türkiye)

Mecit YÖRÜK (Yüzüncü Yıl Üniversitesi, Veteriner Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Van, Türkiye)

Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi

2 0

Yıl: 2011 Cilt: 22 Sayı: 3 Sayfa Aralığı: 169 - 173 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Distribution of plasma cells and alpha-naphthyl acetate esterase – positive lymphocytes in the reproductive organs of female cats in anoestrus, oestrus and pseudopregnancy

Öz:

Kedilerde anöstrüs, östrüs ve yalancı gebelik dönemlerinde dişi genital kanal organları ve ovaryumlarında, plasma hücresi ve alpha naphthyl acetate esterase (ANAE) positif lenfositlerin dağılımı, histokimyasal teknikler kullanılarak ışık mikroskopik olarak belirlendi. Plasma hücrelerinin incelenmesi için doku örnekleri formol-alkol solüsyonunda 48 saat tespit edilip parafinde bloklandı. Altı mikron kalınlığındaki kesitler metil green - pironin ile boyandı. ANAE için doku örnekleri formol-sükroz solüsyonunda (pH 6.8) +4C ‘de 22 saat, sonra Holt solüsyonunda yine aynı şartlarda 22 saat fikse edildi. ANAE boyaması için 8 μm kalınlığındaki kriyostat kesitleri pH 6.2’de inkübe edildi. Kedilerde anöstrüs döneminde plasma hücre sayısı korpus uteride kornu uteriye göre anlamlı derecede azdı (P<0.05). Yalancı gebelik grubundaki kedilerin ovaryum korteks ve medullasında, plasma hücre sayısının, anöstrus ve östrus gruplarına göre daha yüksek olduğu belirlendi (P<0.05). ANAE-pozitif lenfositlerin yoğunluğu, östrus ve yalancı gebelik dönemi kedilerin uterus ve ovaryumlarında anöstrus grubu kedilerden anlamlı derecede yüksekti (P<0.05). Sonuçta, östrüs siklusu ve yalancı gebeliğin dişi kedilerde üreme kanal organları ile ovaryumlarda plasma hücreleri ve ANAE-positif lenfositlerin dağılımındaki değişimlerden sorumlu olabileceği kanısına varıldı.

Anahtar Kelime: lenfositler dölyatağı kızgınlık yumurtalıklar anöstrüs plazma hücreleri doku dağılımı kedi esteraz doku kimyası yalancı gebelik

Konular: Veterinerlik

Östrüs, anöstrüs ve yalancı gebelikteki kedilerin dişi üreme organlarında plazma hücreleri ve alpha-naphthyl acetate esterase pozitif lenfositlerin dağılımı

Öz:

The distribution of plasma cells and alpha naphthyl acetate esterase (ANAE) positive lymphocytes in the reproductive tract and ovaries of cats in anoestrus, oestrus and pseudopregnancy was determined using histochemical methods. For plasma cell determination, tissue samples were fixed in formol-alcohol for 48 hours, embedded in paraffin and six-micrometer-thick sections stained with methyl green-pyronine. For ANAE demonstration, tissue samples were fixed in formol-sucrose fixative (pH 6.8), stored for 22 hours at +4 C and then additionally fixed in Holt’s solution for 22 hours at +4 C. Cryostat sections of 8 μm thickness were stained for ANAE activity at pH 6.2. The number of plasma cells was significantly lower (P<0.05) in the uterine corpus than the uterine horn of anoestrus cats. Plasma cell numbers were significantly higher (P<0.05) in the ovarian cortex and medulla of the pseudopregnancy group than in the anoestrus and oestrus groups. The density of ANAE-positive lymphocytes was significantly (P<0.05) greater in the uterus and ovaries of oestrous and pseudopregnancy cats than anoestrous cats. It was concluded that the stage of oestrous cycle and pseudopregnancy could be responsible for changing the distribution of plasma cells and ANAE-positive lymphocytes in reproductive tract tissues and ovaries of female cats.

Anahtar Kelime: cats esterases histochemistry pseudopregnancy lymphocytes uterus oestrus ovaries anoestrus plasma cells tissue distribution

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	KARACA T, ARIKAN Ş, KALENDER H, PİR YAĞCI İ, YORUK M (2011). Distribution of plasma cells and alpha-naphthyl acetate esterase – positive lymphocytes in the reproductive organs of female cats in anoestrus, oestrus and pseudopregnancy. , 169 - 173.
Chicago	KARACA Turan,ARIKAN Şevket,KALENDER Hakan,PİR YAĞCI İLKNUR,YORUK MECIT Distribution of plasma cells and alpha-naphthyl acetate esterase – positive lymphocytes in the reproductive organs of female cats in anoestrus, oestrus and pseudopregnancy. (2011): 169 - 173.
MLA	KARACA Turan,ARIKAN Şevket,KALENDER Hakan,PİR YAĞCI İLKNUR,YORUK MECIT Distribution of plasma cells and alpha-naphthyl acetate esterase – positive lymphocytes in the reproductive organs of female cats in anoestrus, oestrus and pseudopregnancy. , 2011, ss.169 - 173.
AMA	KARACA T,ARIKAN Ş,KALENDER H,PİR YAĞCI İ,YORUK M Distribution of plasma cells and alpha-naphthyl acetate esterase – positive lymphocytes in the reproductive organs of female cats in anoestrus, oestrus and pseudopregnancy. . 2011; 169 - 173.
Vancouver	KARACA T,ARIKAN Ş,KALENDER H,PİR YAĞCI İ,YORUK M Distribution of plasma cells and alpha-naphthyl acetate esterase – positive lymphocytes in the reproductive organs of female cats in anoestrus, oestrus and pseudopregnancy. . 2011; 169 - 173.
IEEE	KARACA T,ARIKAN Ş,KALENDER H,PİR YAĞCI İ,YORUK M "Distribution of plasma cells and alpha-naphthyl acetate esterase – positive lymphocytes in the reproductive organs of female cats in anoestrus, oestrus and pseudopregnancy." , ss.169 - 173, 2011.
ISNAD	KARACA, Turan vd. "Distribution of plasma cells and alpha-naphthyl acetate esterase – positive lymphocytes in the reproductive organs of female cats in anoestrus, oestrus and pseudopregnancy". (2011), 169-173.

APA	KARACA T, ARIKAN Ş, KALENDER H, PİR YAĞCI İ, YORUK M (2011). Distribution of plasma cells and alpha-naphthyl acetate esterase – positive lymphocytes in the reproductive organs of female cats in anoestrus, oestrus and pseudopregnancy. Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi, 22(3), 169 - 173.
Chicago	KARACA Turan,ARIKAN Şevket,KALENDER Hakan,PİR YAĞCI İLKNUR,YORUK MECIT Distribution of plasma cells and alpha-naphthyl acetate esterase – positive lymphocytes in the reproductive organs of female cats in anoestrus, oestrus and pseudopregnancy. Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi 22, no.3 (2011): 169 - 173.
MLA	KARACA Turan,ARIKAN Şevket,KALENDER Hakan,PİR YAĞCI İLKNUR,YORUK MECIT Distribution of plasma cells and alpha-naphthyl acetate esterase – positive lymphocytes in the reproductive organs of female cats in anoestrus, oestrus and pseudopregnancy. Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi, vol.22, no.3, 2011, ss.169 - 173.
AMA	KARACA T,ARIKAN Ş,KALENDER H,PİR YAĞCI İ,YORUK M Distribution of plasma cells and alpha-naphthyl acetate esterase – positive lymphocytes in the reproductive organs of female cats in anoestrus, oestrus and pseudopregnancy. Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi. 2011; 22(3): 169 - 173.
Vancouver	KARACA T,ARIKAN Ş,KALENDER H,PİR YAĞCI İ,YORUK M Distribution of plasma cells and alpha-naphthyl acetate esterase – positive lymphocytes in the reproductive organs of female cats in anoestrus, oestrus and pseudopregnancy. Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi. 2011; 22(3): 169 - 173.
IEEE	KARACA T,ARIKAN Ş,KALENDER H,PİR YAĞCI İ,YORUK M "Distribution of plasma cells and alpha-naphthyl acetate esterase – positive lymphocytes in the reproductive organs of female cats in anoestrus, oestrus and pseudopregnancy." Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi, 22, ss.169 - 173, 2011.
ISNAD	KARACA, Turan vd. "Distribution of plasma cells and alpha-naphthyl acetate esterase – positive lymphocytes in the reproductive organs of female cats in anoestrus, oestrus and pseudopregnancy". Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi 22/3 (2011), 169-173.