Detection of animal species in some meat and meat products by comparatively using DNA Microarray and Real time PCR methods

TEZMEN, Gündüz; Tekiner, İsmail Hakkı; GÖKÇE, İnci; OZPINAR, HAYDAR

Detection of animal species in some meat and meat products by comparatively using DNA Microarray and Real time PCR methods

Haydar ÖZPINAR, (İstanbul Aydın Üniversitesi Gıda Mühendisliği Bölümü, İstanbul, Türkiye)

Gündüz TEZMEN, (Doğan Holding A.Ş, İş Sağlığı ve Güvenliği Grup Başkanlığı, TR 34676 Üsküdar, İstanbul, Türkiye)

İnci GÖKÇE, (İstanbul Aydın Üniversitesi Gıda Mühendisliği Bölümü, İstanbul, Türkiye)

İsmail Hakkı TEKİNER (İstanbul Aydın Üniversitesi Gıda Mühendisliği Bölümü, İstanbul, Türkiye)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

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Yıl: 2013 Cilt: 19 Sayı: 2 Sayfa Aralığı: 245 - 252 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Detection of animal species in some meat and meat products by comparatively using DNA Microarray and Real time PCR methods

Öz:

Et ve et ürünlerinde tür beyanı uygunluğunun tespiti gıda biliminin ilgi alanlarından birisi olup; tüketici hakları ve güvenli gıda temini bakımından önem taşımaktadır. Dünya nüfusunda sürekli artış nedeniyle et ve et ürünlerine talep artırmaktadır. Artışın bu hızda devam etmesi gıda güvenliği ve kaliteyle ilgili sorunları gündeme getirmektedir. Gıda analizlerinde DNA tabanlı moleküler yöntemlerin hızla gelişmesiyle, bu yöntemler gıda güvenliği denetiminde artan oranda tercih edilmektedir. Bu çalışmada İstanbul’ un farklı semtlerinde yerleşik marketler, et şarküterileri ve semt pazarlarında satışa sunulan 73 adet et ve et ürünü örneğinde DNA Mikroarray Teknolojisi tabanlı Chipron LCD-Array Analiz Sistemi kullanılarak tür beyan uygunluğu incelenmiştir. İncelenen örneklerin 39’unda (%53.4) tür beyanı uygunsuzluğu tespit edilmiştir. Rastgele seçilen 11 örnek için İontek Multipleks Fluorion Et Tür İdentifikasyon Kiti ve İontek FDS Tespit Sistemi (Real Time PCR) kullanılarak doğrulama yapılmıştır. Ayrıca, DNA Mikroarray ve Real Time PCR yöntemleri ile elde edilen sonuçlar arasında %100 uygunluk olduğu; bu iki yöntemin et ve et ürünlerinde tür tayini için yaygınlaştırılarak kullanılabileceği görülmüştür.

Anahtar Kelime: et ürünleri saptama teknikler gıda analizleri gıda kalitesi gıda güvenirliği et kalitesi et tüketici hakları polimeraz zincir reaksiyonu

Konular: Veterinerlik

Karşılaştırmalı DNA Mikroarray ve Real time PCR yöntemi kullanılarak bazı et ve et ürünlerinde tür tayini saptanması

Öz:

Determination of animal species in the meat and meat products is one of the interest of food science, it is also important for consumer rights and food safety. Increasing world population has also remarkably impacted the demand for meat and meat products. Based on this fact, issues related to safety and quality in the meat products have brought up in that manner as well. Through DNA based molecular methods are improved in food analysis, it is preferred increasingly in the control of food safety. In this study, a total of 73 samples of the meat and meat products sold in stores, meat selling markets and public bazaars located in different districts of İstanbul province were analyzed for the detection of animal species notified on the label by using Chipron LCD Array Analysis System. The results showed that 39 samples (53.4%) were labelled incorrectly. Randomly selected eleven samples were corrected by Iontek Fluorion Meat Species Identification Kit and FDS Detection System (Real Time PCR). Hence, it was found that the results obtained by DNA Microarray and Real Time PCR methods were identical (100%) with each other, and both methods should extensively be promoted for the detection of animal species in the meat and meat products.

Anahtar Kelime: meat quality meat consumer protection polymerase chain reaction meat products detection techniques food analysis food quality food safety

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Özpınar H, Aydın İH, Klasing KC, Tekiner İH: Interaction of mannan oligosaccharide with immune system “Transport of MOS in to the Lamina Propria” Kafkas Univ Vet Fak Derg, 18 (1): 121-128, 2012.
2. Karakuş K, Aygün T, Alarslan E: Gaziantep ili merkez ilçede kırmızı et tüketim alışkanlıkları. YYÜ Tar Bil Derg, 18 (2): 113-120, 2008.
3. WHO: Global and regional food consumption patterns and trends. Availability and changes in consumption of animal products. http://www.who.int/nutrition/topics/3_foodconsumption/en/index4.html, World Health Organisation, Geneva, 2011.
4. Çınar H, Demir A, Kalanlar Ş, Taşkaya B, van Berkum S, Dellal I, van Berkum S: Agricultural Sectoral Analysis in Turkey and Integration into the EU: Dairy, Tomato, Cereals and Poultry, AERI, Report 171, Ankara, Türkiye, 2007.
5. Wielinga PR, Schlundt J: Food safety: At the center of a one health approach for combating zoonoses. Curr Top Microbiol Immunol, [Epub ahead of print], 2012.
6. Özpınar H, Erhard MH, Aytug N, Özpınar A, Baklacı C, Karamuftuoglu S, Lösch U: Dose-dependent effects of specific egg antibodies in diarrhea of newborn calves. Preventive Vet Med, 27, 67-73, 1996.
7. Mataragas M, Skandamis PN, Drosinos EH: Risk profiles of pork and poultry meat and risk ratings of various pathogen/product combinations. Int J Food Microbiol, 126 (1-2): 1-12, 2008.
8. Zhao C, Ge B, De Villena J, Sudler R, Yeh E, Zhao S, White DG, Wagner D, Meng J: Prevalence of Campylobacter spp., Escherichia coli, and Salmonella serovars in retail chicken, turkey, pork, and beef from the Greater Washington, D.C., area. Appl Environ Microbiol, 67 (12): 5431-5436, 2001.
9. Teo CG: Much meat, much malady: changing perceptions of the epidemiology of hepatitis E. Clin Microbiol Infect, 16 (1): 24-32, 2010.
10. Jackwood DJ, Sommer-Wagner SE: Detection and characterization of infectious bursal disease viruses in broilers at processing. Prev Vet Med, 97 (1): 45-50, 2010.
11. Meslin FX: Public health impact of zooneses and international approaches for their detectionand containment. Veterinaria Italiana, 44 (4): 583-590, 2008.
12. Anon: Türk Gıda Kodeksi. Çiğ Kırmızı Et ve Hazırlanmış Kırmızı Et Karışımları Tebliği. Tebliğ No: 2006/31, Yayımlandığı Resmi Gazete: 07.07.2006/26221, 2006.
13. Anon: Türk Gıda Kodeksi Yönetmeliği Et Ürünleri Tebliği. Tebliğ No: 2000/4, Yayımlandığı Resmi Gazete: 06.02.2009/27133, 2009.
14. Ali FHM, Mahdey EA: Incidence of Brucella species in slaughtered food animals and its edible offal at Beni-suef, Egypt. Global Veterinaria, 5 (5): 248-254, 2010.
15. WHO: Zoonoses and veterinary public health. Bovine Spongiform Encephalopathy (BSE). http://www.who.int/mediacentre/factsheets/fs113/en/.Fact Sheet No: 113, Revised November 2002 (World Health Organisation, Geneva).
16. Lücker E, Biedermann W, Lachhab S, Hensel A: GC/MS detection of central nervous tissues as specified BSE risk material in meat products: validation by an externally controlled blind trial. Anal Bioanal Chem, 398 (5): 2223-2231, 2010.
17. Bowling MB, Belk KE, Nightingale KK, Goodridge LD, Scanga JA, Sofos JN, Tatum JD, Smith GC: Central nervous system tissue in meat products: an evaluation of risk, prevention strategies, and testing procedures. Adv Food Nutr Res, 53, 39-64, 2007.
18. Güllüce A, Kesmen Z, Yetim H: Isıl işlem uygulanmış et karışımlarında Real-Time PCR tekniği ile tür tayini. 1. Ulusal Helal ve Sağlıklı Gıda Kongresi, 19-20 Kasım, Ankara, Türkiye, s. 196-197, 2011.
19. Commission Directive 2002/86/EC. L 305/19. Official Journal of the European Communities, 2002.
20. Commission Recommendation 2004/787/EC. L 348/18. Official Journal of the European Union, 2004.
21. Hvass A: Species differentiation in minced meat products by immunodiffusion. “biochemical identification of meat species”, Elsevier Applied Science Publishers Ltd., 53-58, England, 1985.
22. Unajak S, Meesawat P, Anyamaneeratch K, Anuwareepong D, Srikulnath K, Choowongkomon K: Identification of species (meat and blood samples) using nested-PCR analysis of mitochondrial DNA. Afr J Biotechnol, 10 (29): 5670-5676, 2011.
23. Türkyılmaz Ö, Irmak H: Et ve et ürünlerinde ELISA Tekniği ile türlerin tespiti. Bornova Vet Kont Araşt Enst Derg, 30 (44): 27-31, 2008.
24. Günşen U, Aydın A, Ovalı B, Coşkun Yİ: Çiğ et ve ısıl işlem görmüş et ürünlerinde ELISA tekniği ile farklı et türlerinin tespiti. İstanbul Üniv Vet Fak Derg, 32 (2): 45-52, 2006.
25. Ayaz Y, Ayaz ND, Erol I: Detection of species in meat and meat products using enzyme-linked immunosorbent assay. J Muscle Foods, 17 (2): 214-220, 2006.
26. Hsieh YHP, Woodward BB, Ho SH: Detection of species substitution in raw and cooked meats using immunoassays. J. Food Protect, 58, 555-559, 1995.
27. Hsieh YHP, Johnson MA, Wetzstein CJ, Gren NR: Detection of species adulteration in pork products using agar-gel immunodiffusion and enzyme-linked immunosorbentassay. J Food Quality, 19, 1-13, 1996.
28. Silvestre MH: La calidad carnes frescas picadas de bovino, ovino, porcino y similares. Alimentaria, 33, 83-85, 1995.
29. Macedo-Siva A, Barbosa SFC, Aklim MGA, Vaz AJ, Shimokomaki V, Tenuta-Filho A: Hamburger meat identification by dot-ELISA. Meat Sci, 56, 189-192, 2000.
30. Myers MJ, Farrell DE, Deaver CM, Mason J, Swaim HL, Yancy HF: Detection of rendered meat and bone meals by PCR is dependent on animal species of origin and DNA extraction method. J Food Prot, 73 (6): 1090-1006, 2010.
31. Özpinar H, Tekiner İH, Gökçe İ: Comparison of LCD-Array and real time PCR techniques for detection and verification of animal species in meat products. 3rd National Food Safety and Security Congress. 3-4 May, İstanbul, Turkey, p. 56, 2012.
32. Demirkoparanoğlu İ, Uysal Ş: Et tür tayininde Real-Time PCR uygulamaları. Gıdalab Bülten, Özel Sayı, No: 1, 2010.
33. Yetim H, Kesmen Z, Şahin F: Kayseri ve Erzurum piyasasında satılan et ürünlerinde farklı hayvan türlerine ait etlerin PCR tekniği kullanılarak belirlenmesi üzerine bir araştırma. Türkiye 9. Gıda Kongresi, 24-26 Mayıs, Bolu, Türkiye, s. 985-988, 2006.
34. Ballin NZ: Authentication of meat and meat products. Meat Sci, 86 (3): 577-587, 2010.
35. Weiss J, Gibis M, Schuh V, Salminen H: Advances in ingredient and processing systems for meat and meat products. Meat Sci, 86 (1): 196-213, 2010.
36. İlhak Oİ, Arslan A: Rastgele çoğaltılmış polimorfik DNA yöntemiyle kanatlı etlerinde tür tayini. http://veteriner.fusabil.org/pdf/pdf_FUSABIL_ 520.pdf, Erişim tarihi: 04.09.2007.
37. Jonker KM, Tilburg JJ, Hagele GH, de Boer E: Species identification in meat products using real-time PCR. Food Addit Contam, 25 (5): 527-533, 2008.
38. Fumière O, Veys P, Boix A, von Holst C, Baeten V, Berben G: Methods of detection, species identification and quantification of processed animal proteins in feedingstuffs. Biotechnol Agron Soc Environ, 13 (S): 59-70, 2009.
39. Ashoor SH, Monte WC, Stiles PG: Liquid chromatographic identification of meats. J Assoc Off Anal Chem, 71 (2): 397-403, 1988.
40. Chou CC, Lin SP, Lee KM, Hsu CT, Vickroy TW, Zen JM: Fast differentiation of meats from fifteen animal species by liquid chromatography with electrochemical detection using copper nanoparticle plakad electrodes. J Chromatogr B Analyt Technol Biomed Life Sci, 846 (1-2): 230-239, 2007.
41. Fukal L: Modern immunoassays in meat-product analysis. Nahrung, 35 (5): 431-48, 1991.
42. Lamber U, Ergin Ö: Fermente Türk sucuklarinda et orijininin indirekt kompetatif ELISA ile belirlenmesi. Erciyes Üniv Vet Fak Derg, 6 (1): 21-29, 2009.
43. Pickering K, Griffin M, Smethurst P, Hargin KD, Stewart CA: Investigation of methods to detect mechanically recovered meat in meat products - IV: Immunology. Meat Sci, 40 (3): 327-336, 1995.
44. Bottero MT, Dalmasso A: Animal species identification in food products: Evolution of biomolecular methods. Vet J, 190 (1): 34-8, 2010.
45. Liu-Stratton Y, Roy S, Sen CK: DNA microarray technology in nutraceutical and food safety. Toxicol Lett, 150 (1): 29-42, 2004.
46. Kato H, Saito K, Kimura T: A perspective on DNA microarray technology in food and nutritional science. Curr Opin Clin Nutr Metab Care, 8 (5): 516-522, 2005.
47. Kostrzynska M, Bachand A: Application of DNA microarray technology for detection, identification, and characterization of food-borne pathogens. Can J Microbiol, 52 (1): 1-8, 2006.
48. Budak ŞÖ, Dönmez S: Gıda biliminde yeni omik teknolojileri. Gıda, 37 (3): 173-179, 2012.
49. Hellberg RS, Morrissey MT: Advances in DNA-based techniques for the detection of seafood species substitution on the commercial market. J Lab Autom, 16 (4): 308-321, 2011.
50. Peter C, Nieweler CB, Cammann K, Börchers T: Differentiation of animal species in food by oligonucleotide microarray hybridization. European Food Research and Technology, 219 (3): 286-293, 2004.
51. New Scientist: DNA chip will catch beefed up chicken. http://www.newscientist.com/article/mg18124372.000-dna-chip-will-catch-beefed-up-chicken.html, Accessed: 6 March 2004.
52. Pereira F, Carneiro J, Amorim A: Identification of species with DNA-based technology: Current progress and challenges. Recent Pat DNA Gene Seq, 2, 187-200, 2008.
53. Zhang L, Zhang L, Liu SC, Zhang YJ, Han Y: DNA-based methods for identification of seafood species. Yi Chuan, 32 (6): 555-560, 2010.
54. Gizzi G, van Raamsdonk LW, Baeten V, Murray I, Berben G, Brambilla G, von Holst C: An overview of tests for animal tissues in feeds applied in response to public health concerns regarding bovine spongiform encephalopathy. Rev Sci Tech, 22 (1): 311-331, 2003.
55. Teletchea F, Maudet C, Hänni C: Food and forensic molecular identification: update and challenges. Trends Biotechnol, 23 (7): 359-366, 2005.
56. Pereira F, Carneiro J, Amorim A: Identification of species with DNA-based technology: current progress and challenges. Recent Pat DNA Gene Seq, 2 (3): 187-199, 2008.
57. Cansu M: Mikroarray Yöntemi ve Virolojideki Uygulamaları. Bitirme Tezi, Karadeniz Teknik Üniv. Fen Fak. Biyoloji Bölümü, 2011.
58. Yoltaş A, Karaboz İ: DNA mikroarray teknolojisi ve uygulama alanları. Elektronik Mikrobiyoloji Dergisi, 8 (1): 01-19, 2010.
59. Saraçlı MA: DNA chip teknolojisi ve mikolojide uygulama alanları. İnfeksiyon Dergisi, 21, 181-184, 2007.
60. Miller MB, Tang YW: Basic concepts of mikroarrays and potential applications in clinical microbiology. Clin. Microbiol Rev, 22 (4): 611-633, 2009.
61. Turan B: İstanbul İli sınırları içinde satışa sunulan sebze örneklerinde Escherichia coli’nin Real Time PCR yöntemi ile Shiga benzeri toksin genleri varlığı bakımından incelenmesi. Yüksek Lisans Tezi, İstanbul Aydın Üniv. Fen Bil. Enst., 2012.
62. Azuka I, Ingrid H, Georg H, Andreas M, Ulrich B: Biochip technology for the detection of animal species in meat products. Food Analytical Methods, 4 (3): 389-398, 2011.
63. Chipron: Meat Species 1.6 LCD Array Kit DNA based identification of meat & poultry species. User Guide for Application, http://www.chipron.com/, Berlin, Germany, 2011.
64. İontek: Fluorion Meat Spec. Ident. QLP 1.0. Fluorion Deteksiyon Sistemi ile Araştırma Amaçlı Kullanım için Kullanım Kılavuzu. Doküman Kodu: F0560102v001, İstanbul, Türkiye, 2011.
65. Chisholm J, Conyers C, Booth C, Lawley W, Hird H: The detection of horse and donkey using real-time PCR. Meat Sci, 70 (4): 727-732, 2005.
66. İlhak İ, Arslan A: Random amplified polymorfic DNA (RAPD) yöntemiyle sığır, koyun, keçi ve yabani domuz etinin ayırt edilmesi. FÜ Sağ Bil Derg, 17 (1): 59-63, 2003.
67. Ferri G, Alù M, Corradini B, Licata M, Beduschi G: Species identification through DNA “barcodes”. Genet Test Mol Biomarkers, 13 (3): 421-426, 2009.
68. Montiel-Sosa JF, Ruiz-Pesini E, Montoya J, Roncalés P, López-Pérez MJ, Pérez-Martos A: Direct and highly species-specific detection of pork meat and fat in meat products by PCR amplification of mitochondrial DNA. J Agric Food Chem, 48 (7): 2829-2832, 2000.
69. Mahajan MV, Gadekar YP, Dighe VD, Kokane RD, Bannalikar AS: Molecular detection of meat animal species targeting MT 12S rRNA gene. Meat Sci, 88 (1): 23-27, 2011.
70. Sun YL, Lin CS: Establishment and application of a fluorescent polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for identifying porcine, caprine, and bovine meats. J Agric Food Chem, 51 (7): 1771-1776, 2003.
71. Rodríguez MA, García T, González I, Asensio L, Hernández PE, Martín R: PCR identification of beef, sheep, goat, and pork in raw and heat-treated meat mixtures. J Food Prot, 67 (1): 172-177, 2004.
72. Martín I, García T, Fajardo V, López-Calleja I, Rojas M, Pavón MA, Hernández PE, González I, Martín R: Technical note: detection of chicken, turkey, duck, and goose tissues in feedstuffs using species-specific polymerase chain reaction. J Anim Sci, 85 (2): 452-458, 2007.
73. Rasooly A, Herold KE: Food microbial pathogen detection and analysis using DNA microarray technologies. Foodborne Pathog Dis, 5 (4): 531-550, 2008.
74. Al-Khaldi SF, Martin SA, Rasooly A, Evans JD: DNA microarray technology used for studying foodborne pathogens and microbial habitats. J AOAC Int, 85 (4): 906-910, 2002.
75. Kostrzynska M, Bachand A: Application of DNA microarray technology for detection, identification, and characterization of food-borne pathogens. Can J Microbiol, 52 (1): 1-8, 2006.

APA	OZPINAR H, TEZMEN G, GÖKÇE İ, Tekiner İ (2013). Detection of animal species in some meat and meat products by comparatively using DNA Microarray and Real time PCR methods. , 245 - 252.
Chicago	OZPINAR HAYDAR,TEZMEN Gündüz,GÖKÇE İnci,Tekiner İsmail Hakkı Detection of animal species in some meat and meat products by comparatively using DNA Microarray and Real time PCR methods. (2013): 245 - 252.
MLA	OZPINAR HAYDAR,TEZMEN Gündüz,GÖKÇE İnci,Tekiner İsmail Hakkı Detection of animal species in some meat and meat products by comparatively using DNA Microarray and Real time PCR methods. , 2013, ss.245 - 252.
AMA	OZPINAR H,TEZMEN G,GÖKÇE İ,Tekiner İ Detection of animal species in some meat and meat products by comparatively using DNA Microarray and Real time PCR methods. . 2013; 245 - 252.
Vancouver	OZPINAR H,TEZMEN G,GÖKÇE İ,Tekiner İ Detection of animal species in some meat and meat products by comparatively using DNA Microarray and Real time PCR methods. . 2013; 245 - 252.
IEEE	OZPINAR H,TEZMEN G,GÖKÇE İ,Tekiner İ "Detection of animal species in some meat and meat products by comparatively using DNA Microarray and Real time PCR methods." , ss.245 - 252, 2013.
ISNAD	OZPINAR, HAYDAR vd. "Detection of animal species in some meat and meat products by comparatively using DNA Microarray and Real time PCR methods". (2013), 245-252.

APA	OZPINAR H, TEZMEN G, GÖKÇE İ, Tekiner İ (2013). Detection of animal species in some meat and meat products by comparatively using DNA Microarray and Real time PCR methods. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 19(2), 245 - 252.
Chicago	OZPINAR HAYDAR,TEZMEN Gündüz,GÖKÇE İnci,Tekiner İsmail Hakkı Detection of animal species in some meat and meat products by comparatively using DNA Microarray and Real time PCR methods. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 19, no.2 (2013): 245 - 252.
MLA	OZPINAR HAYDAR,TEZMEN Gündüz,GÖKÇE İnci,Tekiner İsmail Hakkı Detection of animal species in some meat and meat products by comparatively using DNA Microarray and Real time PCR methods. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.19, no.2, 2013, ss.245 - 252.
AMA	OZPINAR H,TEZMEN G,GÖKÇE İ,Tekiner İ Detection of animal species in some meat and meat products by comparatively using DNA Microarray and Real time PCR methods. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2013; 19(2): 245 - 252.
Vancouver	OZPINAR H,TEZMEN G,GÖKÇE İ,Tekiner İ Detection of animal species in some meat and meat products by comparatively using DNA Microarray and Real time PCR methods. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2013; 19(2): 245 - 252.
IEEE	OZPINAR H,TEZMEN G,GÖKÇE İ,Tekiner İ "Detection of animal species in some meat and meat products by comparatively using DNA Microarray and Real time PCR methods." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 19, ss.245 - 252, 2013.
ISNAD	OZPINAR, HAYDAR vd. "Detection of animal species in some meat and meat products by comparatively using DNA Microarray and Real time PCR methods". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 19/2 (2013), 245-252.