Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the efects of ozone therapy

Aydos, Tolga Reşat; Kisa, Ucler; BAŞAR, Murad Mehmet; ALPCAN, Serhan; Basar, Halil; KUL, Oğuz

Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the efects of ozone therapy

Serhan ALPCAN, (Kırıkkale Üniversitesi, Tıp Fakültesi, Üroloji Anabilim Dalı, Kırıkkale, Türkiye)

Halil BAŞAR, (Kırıkkale Üniversitesi, Tıp Fakültesi, Üroloji Anabilim Dalı, Kırıkkale, Türkiye)

Tolga Reşat AYDOS, (Başkent Üniversitesi, Tıp Fakültesi, Farmakoloji Anabilim Dalı, Ankara, Türkiye)

Oğuz KUL, (Kırıkkale Üniversitesi, Tıp Fakültesi, Patoloji Anabilim Dalı, Kırıkkale, Türkiye)

Üçler KISA, (Kırıkkale Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Kırıkkale, Türkiye)

Murad Mehmet BAŞAR (Kırıkkale Üniversitesi, Tıp Fakültesi, Üroloji Anabilim Dalı, Kırıkkale, Türkiye)

Türk Üroloji Dergisi/Turkish Journal of Urology

5 0

Yıl: 2014 Cilt: 40 Sayı: 4 Sayfa Aralığı: 199 - 206 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the efects of ozone therapy

Öz:

Amaç: Rat testiküler dokusunda vazektomi sonrası serum ve seminal plazma seks hormonları düzeyi yanısıra endotelyal nitrik oksit sentaz (eNOS) ve indüklenebilir nitrik oksit sentaz (iNOS) ekspresyonu değişiklikleri ve apoptotik indeksi değişikleri ve ozon terapinin (OT) etkisini araştırmak.Gereç ve yöntemler: Yetişkin erkek Wistar sıçanları (n=6 her grupta) kullanıldı. Kontrol (G1), 4 hafta (G2)ve 6 hafta (G3) sham operasyonu, sol vazektomi sonrası 4. hafta (G4) ve 6.haftada (G5) orşiektomi, bilateralvazektomi sonrası 4. hafta (G6) ve 6. haftada (G7) orkiektomi, plasebo ikili vazektomi, sol (G8) ve bilateral(G9) vazektomi sonrası 6 hafta ozon terapi sonrası orşiektomi 6 hafta sonra ve 6 hafta OT (G10) sonrasıorşiektomi grupları oluşturuldu.Bulgular: G4 ve G5de sol testiste eNOS ve iNOS immünoreaktivitesinde ve apoptotik indekslerde artışvarken karşı testiste hiçbir değişiklik gözlenmedi. OT G8in sol testisinde ve 9un her iki testisinde bu parametreleri inhibe ederken, G6 ve G7 artış izlendi. Seks hormon düzeyleri vazektomi ve ozon tedavisi sonrasıherhangi bir değişiklik izlenmedi.Sonuç: OT stres koşulları altında yukarıda bahsedilen bazı parametrelere karşı koruyucu olduğu tespit edilirken, sağlıklı koşullarda uygulandığında bazı zararlı etkilere neden olabileceği gözlendi.

Anahtar Kelime:

Konular: Üroloji ve Nefroloji

Vazektomi sonrası rat testiküler dokusunda apoptois: eNOS, iNOS immünoreaktivitesi ve ozon terapinin etkilerinin incelenmesi

Öz:

Objective: We aimed to investigate the changes in endothelial nitric oxide synthase (eNOS) and induciblenitric oxide synthase (iNOS) expression and apoptotic index in rat testicular tissue, as well as serum andseminal plasma sex hormone levels after vasectomy, and the effect of ozone therapy (OT).Material and methods: Adult male Wistar rats were used (n=6 per group). Control (G1), sham for 4 weeks(G2) or 6 weeks (G3), orchiectomy at the 4 th (G4) or 6 th (G5) week after left vasectomy, orchiectomy at the4 th (G6) or 6 th (G7) week after bilateral vasectomy, orchiectomy after 6 weeks OT following left (G8) orbilateral (G9) vasectomy, orchiectomy after 6 weeks OT (G10).Results: In the left testes, while there were increases in eNOS and iNOS immunoreactivity and apoptoticindexes in G4 and G5, no changes were observed in contralateral testis. These values increased in G6 andG7, while OT inhibited these parameters in the left testis of G8 and both testes of G9. Sex hormone levelsdid not show any changes after vasectomy and ozone therapy.Conclusion: While OT was found to be protective against some parameters mentioned above under stressconditions, it seemed to cause some harmful effects when used in healthy conditions.

Anahtar Kelime:

Konular: Üroloji ve Nefroloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	ALPCAN S, Basar H, Aydos T, KUL O, Kisa U, BAŞAR M (2014). Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the efects of ozone therapy. , 199 - 206.
Chicago	ALPCAN Serhan,Basar Halil,Aydos Tolga Reşat,KUL Oğuz,Kisa Ucler,BAŞAR Murad Mehmet Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the efects of ozone therapy. (2014): 199 - 206.
MLA	ALPCAN Serhan,Basar Halil,Aydos Tolga Reşat,KUL Oğuz,Kisa Ucler,BAŞAR Murad Mehmet Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the efects of ozone therapy. , 2014, ss.199 - 206.
AMA	ALPCAN S,Basar H,Aydos T,KUL O,Kisa U,BAŞAR M Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the efects of ozone therapy. . 2014; 199 - 206.
Vancouver	ALPCAN S,Basar H,Aydos T,KUL O,Kisa U,BAŞAR M Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the efects of ozone therapy. . 2014; 199 - 206.
IEEE	ALPCAN S,Basar H,Aydos T,KUL O,Kisa U,BAŞAR M "Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the efects of ozone therapy." , ss.199 - 206, 2014.
ISNAD	ALPCAN, Serhan vd. "Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the efects of ozone therapy". (2014), 199-206.

APA	ALPCAN S, Basar H, Aydos T, KUL O, Kisa U, BAŞAR M (2014). Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the efects of ozone therapy. Türk Üroloji Dergisi/Turkish Journal of Urology, 40(4), 199 - 206.
Chicago	ALPCAN Serhan,Basar Halil,Aydos Tolga Reşat,KUL Oğuz,Kisa Ucler,BAŞAR Murad Mehmet Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the efects of ozone therapy. Türk Üroloji Dergisi/Turkish Journal of Urology 40, no.4 (2014): 199 - 206.
MLA	ALPCAN Serhan,Basar Halil,Aydos Tolga Reşat,KUL Oğuz,Kisa Ucler,BAŞAR Murad Mehmet Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the efects of ozone therapy. Türk Üroloji Dergisi/Turkish Journal of Urology, vol.40, no.4, 2014, ss.199 - 206.
AMA	ALPCAN S,Basar H,Aydos T,KUL O,Kisa U,BAŞAR M Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the efects of ozone therapy. Türk Üroloji Dergisi/Turkish Journal of Urology. 2014; 40(4): 199 - 206.
Vancouver	ALPCAN S,Basar H,Aydos T,KUL O,Kisa U,BAŞAR M Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the efects of ozone therapy. Türk Üroloji Dergisi/Turkish Journal of Urology. 2014; 40(4): 199 - 206.
IEEE	ALPCAN S,Basar H,Aydos T,KUL O,Kisa U,BAŞAR M "Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the efects of ozone therapy." Türk Üroloji Dergisi/Turkish Journal of Urology, 40, ss.199 - 206, 2014.
ISNAD	ALPCAN, Serhan vd. "Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the efects of ozone therapy". Türk Üroloji Dergisi/Turkish Journal of Urology 40/4 (2014), 199-206.