Investigation of mast cell distribution in the ovine oviduct during oestral and luteal phases of the qestrous cycles

ÖZCAN, Ziya; ÖZEN, Asuman; Karahan, Siyami; KÜRÜM, Aytül

Investigation of mast cell distribution in the ovine oviduct during oestral and luteal phases of the qestrous cycles

Aytül KÜRÜM, (Kırıkkale Üniversitesi, Veteriner Fakültesi, Histoloji Embriyoloji Bilim Dalı, Kırıkkale, Türkiye)

Asuman ÖZEN, (Ankara Üniversitesi, Veteriner Fakültesi, Histoloji Embriyoloji Anabilim Dalı, Ankara, Türkiye)

Siyami KARAHAN, (Kırıkkale Üniversitesi, Veteriner Fakültesi, Histoloji Embriyoloji Bilim Dalı, Kırıkkale, Türkiye)

Ziya ÖZCAN (Ankara Üniversitesi, Veteriner Fakültesi, Histoloji Embriyoloji Anabilim Dalı, Ankara, Türkiye)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

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Yıl: 2014 Cilt: 20 Sayı: 6 Sayfa Aralığı: 915 - 920 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Investigation of mast cell distribution in the ovine oviduct during oestral and luteal phases of the qestrous cycles

Öz:

Mast hücreleri birçok organ ve sistemdeki çeşitli fizyolojik süreçlerde önemli rolleri bulunan heterojen hücre topluluğudur.Bu çalışmada koyun ovidukt mukozasındaki mast hücrelerini boyanma özellikleri ve ince yapı düzeyinde incelemeyi amaçladık.Mezbahanede kesim sırasında östral ve luteal dönemleri Akkaraman koyunlarının oviduct örnekleri alındı. Alınan örnekler %10 formolve IFAA ile tespit edilerek Toluidin blue ve Alcian blue-Safranin O (Ab/SO) ile boyandı. Mast hücrelerinin, kan damarlarının ve oviduktepitelinin bazal membranına yakın olarak yerleştiği görüldü. IFAA ile tespit edilen dokulardaki mast hücre sayısının %10 formolletespit edilen dokulara göre daha fazla olduğu belirlendi (P=0.003). Dikkat çekici olarak mast hücrelerinin tamamının Ab(+) ve SO(-)olduğu görüldü. Luteal ve östral dönemler arasında mast hücre sayısı yönünden fark bulunamadı (P>0.05). Fakat ovidukt bölgeleriarasında istatistiksel olarak önemli fark vardı ve mast hücre sayısı istmusta daha azdı (P=0.006). Elektron mikroskobik incelemelerdemast hücrelerinin elektron açık ve elektron koyu olmak üzere iki tip granül içerdiği ortaya konuldu. Bazı elektron açık granüllerdeeksantrik yerleşimli elektron koyu kristal benzeri bir yapının varlığı gözlendi. İstmusta mast hücrelerinin daha az sayıda görülmesininve granüllerdeki eksantrik yerleşimli kristal benzeri yapının daha sonraki çalışmalarda araştırılması önemlidir.

Anahtar Kelime:

Konular: Veterinerlik

Östral ve luteal dönemlerdeki koyunların oviduktunda mast hücrelerinin incelenmesi

Öz:

Mast cells are heterogeneous cell populations that play significant roles in many organs and systems and involve variousphysiological processes. We aimed to evaluate mast cells in the ovine oviduct mucosa by means of their staining and ultrastructuralcharacteristics. The ovine oviduct samples of Akkaraman breed were collected from the slaughterhouse and they are categorizedas luteal and oestral phases. They were fixed either with 10% formalin or IFAA and stained with Toluidine blue and Alcian blueand Safranin O (Ab/SO). Mast cells were located near blood vessels and basal membrane. Compared to 10% formalin fixed tissues,the number of mast cells were higher in IFAA fixed tissues (P=0.003). Importantly all mast cells Ab(+) and SO(-) so that they werecategorized as mucosal type. The number of mast cells did not difer between luteal and oestral phases (P>0.05). However, there weresignificant diferences among diferent regions of the oviduct with a less count in the isthmus regions (P=0.006). Transmission electronmicroscopy revealed that the oviduct mast cells contained two types of granules: an electron lucent, electron dense. Some electronlucent granules contained an eccentrically located crystal-like structure. The significance of less mast cell counts in the isthmus and theeccentrically located single crystal-like structure should be further investigated in future studies.

Anahtar Kelime:

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	KÜRÜM A, ÖZEN A, Karahan S, ÖZCAN Z (2014). Investigation of mast cell distribution in the ovine oviduct during oestral and luteal phases of the qestrous cycles. , 915 - 920.
Chicago	KÜRÜM Aytül,ÖZEN Asuman,Karahan Siyami,ÖZCAN Ziya Investigation of mast cell distribution in the ovine oviduct during oestral and luteal phases of the qestrous cycles. (2014): 915 - 920.
MLA	KÜRÜM Aytül,ÖZEN Asuman,Karahan Siyami,ÖZCAN Ziya Investigation of mast cell distribution in the ovine oviduct during oestral and luteal phases of the qestrous cycles. , 2014, ss.915 - 920.
AMA	KÜRÜM A,ÖZEN A,Karahan S,ÖZCAN Z Investigation of mast cell distribution in the ovine oviduct during oestral and luteal phases of the qestrous cycles. . 2014; 915 - 920.
Vancouver	KÜRÜM A,ÖZEN A,Karahan S,ÖZCAN Z Investigation of mast cell distribution in the ovine oviduct during oestral and luteal phases of the qestrous cycles. . 2014; 915 - 920.
IEEE	KÜRÜM A,ÖZEN A,Karahan S,ÖZCAN Z "Investigation of mast cell distribution in the ovine oviduct during oestral and luteal phases of the qestrous cycles." , ss.915 - 920, 2014.
ISNAD	KÜRÜM, Aytül vd. "Investigation of mast cell distribution in the ovine oviduct during oestral and luteal phases of the qestrous cycles". (2014), 915-920.

APA	KÜRÜM A, ÖZEN A, Karahan S, ÖZCAN Z (2014). Investigation of mast cell distribution in the ovine oviduct during oestral and luteal phases of the qestrous cycles. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 20(6), 915 - 920.
Chicago	KÜRÜM Aytül,ÖZEN Asuman,Karahan Siyami,ÖZCAN Ziya Investigation of mast cell distribution in the ovine oviduct during oestral and luteal phases of the qestrous cycles. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 20, no.6 (2014): 915 - 920.
MLA	KÜRÜM Aytül,ÖZEN Asuman,Karahan Siyami,ÖZCAN Ziya Investigation of mast cell distribution in the ovine oviduct during oestral and luteal phases of the qestrous cycles. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.20, no.6, 2014, ss.915 - 920.
AMA	KÜRÜM A,ÖZEN A,Karahan S,ÖZCAN Z Investigation of mast cell distribution in the ovine oviduct during oestral and luteal phases of the qestrous cycles. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2014; 20(6): 915 - 920.
Vancouver	KÜRÜM A,ÖZEN A,Karahan S,ÖZCAN Z Investigation of mast cell distribution in the ovine oviduct during oestral and luteal phases of the qestrous cycles. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2014; 20(6): 915 - 920.
IEEE	KÜRÜM A,ÖZEN A,Karahan S,ÖZCAN Z "Investigation of mast cell distribution in the ovine oviduct during oestral and luteal phases of the qestrous cycles." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 20, ss.915 - 920, 2014.
ISNAD	KÜRÜM, Aytül vd. "Investigation of mast cell distribution in the ovine oviduct during oestral and luteal phases of the qestrous cycles". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 20/6 (2014), 915-920.