In this study, the growth, development and infectivity of Toxoplasma gondii, an obligatory intracellular parasite, was investigated in human amnion cell cultures, Cells obtained from human amniotic fluid were cultured in flasks that contained a suitable culture medium. Monolayers were infected with T.gondii tachyzoites that had been obtained from peritoneal exudates of infected mice. After 48 hours, T.gondii organisms in groups and in rosette formation were observed. The cultured cells were ruptured by the proliferation of T.gondii tachyzoites and the released organisms infected other cells. Thirty days later tachyzoites obtained from the supernatant of the culture medium were injected into Balb/c mice all of which were infected. At the end of the third month, we found that there still were viable tachyzoites in the cultures. Thus, T.gondii can increase, develop and live for three months in human amnion cell cultures, We believe that this cell culture can be used for in vitro studies of T.gondii if it is standardized.