Objectives: This study was descriptively conducted in order to determine nurses professional values and affecting factors. Methods: A total of 120 nurses who work at a governmental hospital in a city, who met the study inclusion criteria and who accepted to participate in the study were recruited. Data for the research was collected by Information Form and Professional Values Scale. Results: According to results of the research, it was found that 57.5% of the nurses were in the 30-39 age group, 85.8% were women, 79.2% were married and 56.7% had a graduate degree. In this research, the mean score of nurses professional values was found to be 90.7±14.7. It has been determined that nurses professional values in the 40 year and older group included males that were single, had a master s degree, had administrative tasks, were members of an association, participated in scientific meetings and received training in professional values were higher than other groups but this was not statistically significant (p>0.05). Conclusion: It was determined that mean score of nurses professional values was found to be above the middle level. According to these results, it is suggested that training on professional values should be planned for nurses and comprehensive studies should be conducted.