Effects of Intracisternal and Intravenous Dexmedetomidine on Ischemia-Induced Brain Inj ury in Rat: A Comparative study

ATİLLA, PERGİN; APAN, Alpaslan; BAKAR, BULENT; KÖSE, Emine Arzu; KILINÇ, Kamer; KASIMCAN, Mustafa Ömür; MÜFTÜOĞLU, Sevda Fatma

Effects of Intracisternal and Intravenous Dexmedetomidine on Ischemia-Induced Brain Inj ury in Rat: A Comparative study

Emine Arzu KÖSE, (Kırıkkale Üniversitesi, Tıp Fakültesi, Anesteziyoloji ve Reanimasyon Anabilim Dalı, Kırıkkale, Türkiye)

BÜLENT BAKAR, (Kırıkkale Üniversitesi, Tıp Fakültesi, Nöroşirürji Anabilim Dalı, Kırıkkale, Türkiye)

Mustafa Ömür KASIMCAN, (Kırıkkale Üniversitesi, Tıp Fakültesi, Nöroşirürji Anabilim Dalı, Kırıkkale, Türkiye)

PERGİN ATİLLA, (Hacettepe Üniversitesi, Tıp Fakültesi, Histolojİ ve Embriyoloji Anabilim Dalı, Ankara, Türkiye)

Kamer KILINÇ, (Hacettepe Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Ankara, Türkiye)

Sevda Fatma MÜFTÜOĞLU, (Hacettepe Üniversitesi, Tıp Fakültesi, Histolojİ ve Embriyoloji Anabilim Dalı, Ankara, Türkiye)

Alpaslan APAN (Kırıkkale Üniversitesi, Tıp Fakültesi, Anesteziyoloji ve Reanimasyon Anabilim Dalı, Kırıkkale, Türkiye)

Turkish Neurosurgery

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Yıl: 2013 Cilt: 23 Sayı: 2 Sayfa Aralığı: 208 - 217 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Effects of Intracisternal and Intravenous Dexmedetomidine on Ischemia-Induced Brain Inj ury in Rat: A Comparative study

Öz:

AMAÇ: Sıçanlarda inkomplet serebral iskemisi üzerine intrasisternal yolla verilen deksmedetomidinin etkilerinin intravenöz yolla verilendeksmedetomidinin etkileriyle karşılaştırılması amaçlandı.YÖNTEM ve GEREÇLER: Ortak sağ karotid arter oklüzyonu ve hemoraj ik hipotansiyon ile 30 dakika süreyle serebral iskemi oluşturuldu. İntrasisternal gruplarda, iskemiden 30 dakika önce sisterna magna içine 0,1 ml % 0.9 NaCl (Grup SIC, n=6) veya 9 µg/kg deksmedetomidin(Grup DIC, n=6) verildi.İntravenöz gruplarda ise 9 µg/kg deksmedetomidin (Grup DIV, n=6) veya % 0,9 NaCl (Grup CONTROL, n=6) 5 ml/kg/hhızla 2 saat süreyle infüze edildi. Yirmi dört saat sonra beyin dokusunda ve plazmada lipid peroksidasyon seviyeleri ölçüldü. Histopatoloj ikincelemeler için hipokampal yapı kullanıldı.BULGULAR: İntravenöz deksmedetomidin ortalama arteriyel basınç ve plazma glukoz seviyelerinde bazale göre bir düşüşe neden oldu. GrupDIV ile Grup DIC, Grup SIC ve CONTROL grupları arasında beyin dokusu lipid peroksidasyon seviyeleri ve piknotik hücre sayıları bakımındananlamlı farklılık vardı (sırasıyla; p<0,001, p<0,001, p=0,001 ve p<0,001, p=0,01, p=0,009). Grup DIV için ortalama plazma lipid peroksidasyonseviyeleri Grup DICınkinden farklı bulundu (p=0,003). SONUÇ: İskeminin indüklediği nöronal hasarda sistemik yolla verilen deksmedetomidinin nöroprotektif etkisi varken santral yolla verilendeksmedetomidinin nöroprotektif etkisi yoktur.

Anahtar Kelime:

Konular: Cerrahi

Sıçanlarda İskemiy le İndüklenen Bey in Hasarı Üzerine İntravenöz ve İntrasisternal Deksmedetomidinin Etkileri: Karşılaştırmalı Bir Çalışma

Öz:

AIM: To compare the effect of dexmedetomidine administered by intracisternal route with by intravenous route on brain tissue of rat afterincomplete cerebral ischemia.MATERIAL and METHODS: Cerebral ischemia was produced by the combination of right common carotid artery occlusion and hemorrhagichypotension during 30 minutes. Thirty minutes before the ischemia, 0.1 ml 0.9% NaCl (Group SIC, n=6) or 9 µg/kg dexmedetomidine (GroupDIC, n=6) was administered into the cisterna magna. For the intravenous groups, 9 µg/kg dexmedetomidine (Group DIV, n=6) or 0.9% NaCl(Group CONTROL, n=6) 5 ml/kg/h was given in 2 hours. After 24 hours, the lipid peroxidation levels were measured in the brain tissue andplasma. Hippocampal formations were used for histopathological examination.results: Intravenous dexmedetomidine produced a decrease in baseline mean arterial blood pressure and plasma glucose concentrations.There was a significant difference between the DIV group and DIC, SIC, CONTROL groups regarding the brain lipid peroxidation levels (p<0.001,p<0.001, p=0.001, respectively), and regarding the picnotic neuronal cell count (p<0.001, p=0.01, p=0.009, respectively). Mean plasma lipidperoxidation levels of the DIV group was different from the DIC group (p=0.003). CONCLUSION: Systemically administered dexmedetomidine had neuroprotective effect in ischemia-induced neuronal damage, but centrallyadministered dexmedetomidine did not.

Anahtar Kelime:

Konular: Cerrahi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	KÖSE E, BAKAR B, KASIMCAN M, ATİLLA P, KILINÇ K, MÜFTÜOĞLU S, APAN A (2013). Effects of Intracisternal and Intravenous Dexmedetomidine on Ischemia-Induced Brain Inj ury in Rat: A Comparative study. , 208 - 217.
Chicago	KÖSE Emine Arzu,BAKAR BULENT,KASIMCAN Mustafa Ömür,ATİLLA PERGİN,KILINÇ Kamer,MÜFTÜOĞLU Sevda Fatma,APAN Alpaslan Effects of Intracisternal and Intravenous Dexmedetomidine on Ischemia-Induced Brain Inj ury in Rat: A Comparative study. (2013): 208 - 217.
MLA	KÖSE Emine Arzu,BAKAR BULENT,KASIMCAN Mustafa Ömür,ATİLLA PERGİN,KILINÇ Kamer,MÜFTÜOĞLU Sevda Fatma,APAN Alpaslan Effects of Intracisternal and Intravenous Dexmedetomidine on Ischemia-Induced Brain Inj ury in Rat: A Comparative study. , 2013, ss.208 - 217.
AMA	KÖSE E,BAKAR B,KASIMCAN M,ATİLLA P,KILINÇ K,MÜFTÜOĞLU S,APAN A Effects of Intracisternal and Intravenous Dexmedetomidine on Ischemia-Induced Brain Inj ury in Rat: A Comparative study. . 2013; 208 - 217.
Vancouver	KÖSE E,BAKAR B,KASIMCAN M,ATİLLA P,KILINÇ K,MÜFTÜOĞLU S,APAN A Effects of Intracisternal and Intravenous Dexmedetomidine on Ischemia-Induced Brain Inj ury in Rat: A Comparative study. . 2013; 208 - 217.
IEEE	KÖSE E,BAKAR B,KASIMCAN M,ATİLLA P,KILINÇ K,MÜFTÜOĞLU S,APAN A "Effects of Intracisternal and Intravenous Dexmedetomidine on Ischemia-Induced Brain Inj ury in Rat: A Comparative study." , ss.208 - 217, 2013.
ISNAD	KÖSE, Emine Arzu vd. "Effects of Intracisternal and Intravenous Dexmedetomidine on Ischemia-Induced Brain Inj ury in Rat: A Comparative study". (2013), 208-217.

APA	KÖSE E, BAKAR B, KASIMCAN M, ATİLLA P, KILINÇ K, MÜFTÜOĞLU S, APAN A (2013). Effects of Intracisternal and Intravenous Dexmedetomidine on Ischemia-Induced Brain Inj ury in Rat: A Comparative study. Turkish Neurosurgery, 23(2), 208 - 217.
Chicago	KÖSE Emine Arzu,BAKAR BULENT,KASIMCAN Mustafa Ömür,ATİLLA PERGİN,KILINÇ Kamer,MÜFTÜOĞLU Sevda Fatma,APAN Alpaslan Effects of Intracisternal and Intravenous Dexmedetomidine on Ischemia-Induced Brain Inj ury in Rat: A Comparative study. Turkish Neurosurgery 23, no.2 (2013): 208 - 217.
MLA	KÖSE Emine Arzu,BAKAR BULENT,KASIMCAN Mustafa Ömür,ATİLLA PERGİN,KILINÇ Kamer,MÜFTÜOĞLU Sevda Fatma,APAN Alpaslan Effects of Intracisternal and Intravenous Dexmedetomidine on Ischemia-Induced Brain Inj ury in Rat: A Comparative study. Turkish Neurosurgery, vol.23, no.2, 2013, ss.208 - 217.
AMA	KÖSE E,BAKAR B,KASIMCAN M,ATİLLA P,KILINÇ K,MÜFTÜOĞLU S,APAN A Effects of Intracisternal and Intravenous Dexmedetomidine on Ischemia-Induced Brain Inj ury in Rat: A Comparative study. Turkish Neurosurgery. 2013; 23(2): 208 - 217.
Vancouver	KÖSE E,BAKAR B,KASIMCAN M,ATİLLA P,KILINÇ K,MÜFTÜOĞLU S,APAN A Effects of Intracisternal and Intravenous Dexmedetomidine on Ischemia-Induced Brain Inj ury in Rat: A Comparative study. Turkish Neurosurgery. 2013; 23(2): 208 - 217.
IEEE	KÖSE E,BAKAR B,KASIMCAN M,ATİLLA P,KILINÇ K,MÜFTÜOĞLU S,APAN A "Effects of Intracisternal and Intravenous Dexmedetomidine on Ischemia-Induced Brain Inj ury in Rat: A Comparative study." Turkish Neurosurgery, 23, ss.208 - 217, 2013.
ISNAD	KÖSE, Emine Arzu vd. "Effects of Intracisternal and Intravenous Dexmedetomidine on Ischemia-Induced Brain Inj ury in Rat: A Comparative study". Turkish Neurosurgery 23/2 (2013), 208-217.