Saving the zone of stasis in burns with melatonin: an experimental study in rats

AVUNDUK, Mustafa Cihad; KAYAPINAR, Muhammed; SEYHAN, Nevra; savaci, nedim

Saving the zone of stasis in burns with melatonin: an experimental study in rats

Muhammed KAYAPINAR, (Necmettin Erbakan Üniversitesi, Tıp Fakültesi, Konya, Türkiye)

Nevra SEYHAN, (Necmettin Erbakan Üniversitesi, Tıp Fakültesi, Konya, Türkiye)

Mustafa Cihad AVUNDUK, (Necmettin Erbakan Üniversitesi, Meram Tıp Fakültesi, Patoloji Anabilim Dalı, Konya, Türkiye)

Nedim SAVACI (Necmettin Erbakan Üniversitesi, Tıp Fakültesi, Konya, Türkiye)

Ulusal Travma ve Acil Cerrahi Dergisi

5 1

Yıl: 2015 Cilt: 21 Sayı: 6 Sayfa Aralığı: 419 - 424 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Saving the zone of stasis in burns with melatonin: an experimental study in rats

Öz:

Anahtar Kelime:

Konular: Cerrahi

Öz:

BACKGROUND: Studies aimed at recovering the zone of stasis are one of the major issues of experimental burn studies. Many drugs including antithrombotics, anticoagulants, anti-inflammatories have been investigated experimentally for saving the zone of stasis. In this study, the effect of the systemic melatonin on the zone of stasis was evaluated.METHODS: Twenty Wistar Albino rats were used in the study. Rats were assigned to two groups (n=10). The metal comb 1x2 cm in size was immersed in boiling water and held for 20 seconds on the back of the rats to create burn wounds.No treatment was given to the control group. Melatonin was given at a dose of 10 mg/kg/d by intraperitoneal injection in the treatment group for 7 days. Daily digital photographs of both groups were obtained. Total necrotic burn areas and the zone of stasis were assessed with Auto CAD and Visual Analyzing computer programs. At the end of one week, rats were sacrificed and skin biopsies were taken for histological examination. Edema, congestion, inflammatory infiltration, vascular proliferation and fibrosis were the parameters evaluated. Data were evaluated statistically by Chi-square test and Student-t test.RESULTS: When histopatologic data and the measured values for total necrotic areas and zone of stasis of the experimental group werecompared to control group, the results were statistically significant (p<0.05).CONCLUSION: According to the results of this study, melatonin is efficient in saving the zone of stasis in burns

Anahtar Kelime:

Konular: Cerrahi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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Yanık staz zonunun melatonin kullanımıyla kurtarılması: Sıçanlarda deneysel çalışma
Dr. Muhammed Kayapınar,1 Dr. Nevra Seyhan,1 Dr. Mustafa Cihad Avunduk,2 Dr. Nedim Savacı1
Necmettin Erbakan Üniversitesi Tıp Fakültesi, Plastik Cerrahi Anabilim Dalı, Konya
Necmettin Erbakan Üniversitesi Tıp Fakültesi, Patoloji Anabilim Dalı, Konya
AMAÇ: Staz zonunun kurtarılmasını amaçlayan çalışmalar deneysel yanık çalışmalarının önemli bir bölümünü oluşturmaktadır. Staz zonunun kurta
rılmasında deneysel olarak antitrombotik, antienflamatuvar, antikoagülan gibi birçok ilaç araştırılmıştır. Bu çalışmanın amacı sistemik melatoninin staz
zonuna etkisini değerlendirmektir.
GEREÇ VE YÖNTEM: Çalışmada 20 adet erişkin Wistar Albino sıçan kullanıldı. Randomize seçilen sıçanlar iki gruba ayrıldı (n=10). Sıçanların sırtları
tıraşlandıktan sonra 1x2 cm ebadında kaynar suda ısıtılmış metal plak 20 sn bekletilerek yanık oluşturuldu. Kontrol grubuna tedavi verilmedi. Tedavi
grubuna yedi gün süreyle 10 mg/kg/gün dozunda intraperitoneal melatonin enjeksiyonu yapıldı. Her iki grubun günlük dijital fotoğrafları çekildi.
Total nekrotik yanık alanları ve staz zonu Auto CAD ve görüntü analizi programlarıyla değerlendirildi. Bir hafta sonunda sıçanlar sakrifiye edilerek
histolojik inceleme için cilt biyopsisi alındı. Ödem, konjesyon, enflamatuvar infiltrasyon, vasküler proliferasyon ve fibrozis değerlendirilen paramet
relerdi. Elde edilen veriler ki-kare ve student t-testleri ile değerlendirildi.
BULGULAR: Experimental gruba ait histopatolojik veriler ile total nekrotik yanık alanları ve staz zonu ölçümlerine ait sayısal veriler kontrol grubu ile
karşılaştırıldığında melatoninin staz zonuna etkinliğini kanıtlayacak şekilde istatistiksel olarak anlamlıydı (p_____0.05).
TARTIŞMA: Bu çalışmanın sonucuna göre yanıkta staz zonunun kurtarılmasında melatonin etkilidir.
Anahtar sözcükler: Melatonin; staz zonu; yanık.
Ulus Travma Acil Cerrahi Derg 2015;21(6):419-424 doi: 10.5505/tjtes.2015.53059

APA	KAYAPINAR M, SEYHAN N, AVUNDUK M, savaci n (2015). Saving the zone of stasis in burns with melatonin: an experimental study in rats. , 419 - 424.
Chicago	KAYAPINAR Muhammed,SEYHAN Nevra,AVUNDUK Mustafa Cihad,savaci nedim Saving the zone of stasis in burns with melatonin: an experimental study in rats. (2015): 419 - 424.
MLA	KAYAPINAR Muhammed,SEYHAN Nevra,AVUNDUK Mustafa Cihad,savaci nedim Saving the zone of stasis in burns with melatonin: an experimental study in rats. , 2015, ss.419 - 424.
AMA	KAYAPINAR M,SEYHAN N,AVUNDUK M,savaci n Saving the zone of stasis in burns with melatonin: an experimental study in rats. . 2015; 419 - 424.
Vancouver	KAYAPINAR M,SEYHAN N,AVUNDUK M,savaci n Saving the zone of stasis in burns with melatonin: an experimental study in rats. . 2015; 419 - 424.
IEEE	KAYAPINAR M,SEYHAN N,AVUNDUK M,savaci n "Saving the zone of stasis in burns with melatonin: an experimental study in rats." , ss.419 - 424, 2015.
ISNAD	KAYAPINAR, Muhammed vd. "Saving the zone of stasis in burns with melatonin: an experimental study in rats". (2015), 419-424.

APA	KAYAPINAR M, SEYHAN N, AVUNDUK M, savaci n (2015). Saving the zone of stasis in burns with melatonin: an experimental study in rats. Ulusal Travma ve Acil Cerrahi Dergisi, 21(6), 419 - 424.
Chicago	KAYAPINAR Muhammed,SEYHAN Nevra,AVUNDUK Mustafa Cihad,savaci nedim Saving the zone of stasis in burns with melatonin: an experimental study in rats. Ulusal Travma ve Acil Cerrahi Dergisi 21, no.6 (2015): 419 - 424.
MLA	KAYAPINAR Muhammed,SEYHAN Nevra,AVUNDUK Mustafa Cihad,savaci nedim Saving the zone of stasis in burns with melatonin: an experimental study in rats. Ulusal Travma ve Acil Cerrahi Dergisi, vol.21, no.6, 2015, ss.419 - 424.
AMA	KAYAPINAR M,SEYHAN N,AVUNDUK M,savaci n Saving the zone of stasis in burns with melatonin: an experimental study in rats. Ulusal Travma ve Acil Cerrahi Dergisi. 2015; 21(6): 419 - 424.
Vancouver	KAYAPINAR M,SEYHAN N,AVUNDUK M,savaci n Saving the zone of stasis in burns with melatonin: an experimental study in rats. Ulusal Travma ve Acil Cerrahi Dergisi. 2015; 21(6): 419 - 424.
IEEE	KAYAPINAR M,SEYHAN N,AVUNDUK M,savaci n "Saving the zone of stasis in burns with melatonin: an experimental study in rats." Ulusal Travma ve Acil Cerrahi Dergisi, 21, ss.419 - 424, 2015.
ISNAD	KAYAPINAR, Muhammed vd. "Saving the zone of stasis in burns with melatonin: an experimental study in rats". Ulusal Travma ve Acil Cerrahi Dergisi 21/6 (2015), 419-424.